Public release date: 3-Oct-2013 [ | E-mail | Share ]
Contact: Dan Ferber dan.ferber@wyss.harvard.edu 617-432-1547 Wyss Institute for Biologically Inspired Engineering at Harvard
A team at the Wyss Institute for Biologically Inspired Engineering at Harvard University has been awarded a special $3.5 million grant from the National Institutes of Health (NIH) to develop an inexpensive and easy-to-use new microscopy method to simultaneously spot many tiny components of cells.
The grant, called a Transformative Research Award, is part of an NIH initiative to fund high-risk, high-reward research, and in 2013 the agency funded just 10 of these projects nationally.
The DNA-based microscopy method could potentially lead to new ways of diagnosing disease by distinguishing healthy and diseased cells based on sophisticated molecular details. It could also help scientists uncover how the cell's components carry out their work inside the cell.
"If you want to study physiology and disease, you want to see how the molecules work, and it's important to see them in their native environments," said Peng Yin, Ph.D., a core faculty member at the Wyss Institute and Assistant Professor of Systems Biology at Harvard Medical School. Yin will lead the project, and he will collaborate with Samie Jaffrey, M.D., Ph.D., a Professor of Pharmacology at Weill Cornell Medical College, and Ralf Jungmann, Ph.D., a postdoctoral scholar in Yin's Wyss Institute lab, among others.
Biologists have used microscopes to reveal how tiny structures inside cells prop them up and help them move, reproduce, activate genes, and much more. But although microscope makers have honed the technology for centuries to get ever-clearer images, they have been limited by the laws of physics. When two objects are closer than about 0.2 micrometers, or about one five-hundredth the width of a human hair, the scientists can no longer distinguish them using traditional light microscopes. As a result, the viewer sees one blurry blob where in reality there are two objects. This occurs because of the way light rays bend around objects, and is known as the diffraction limit.
Molecules such as enzymes, receptors, RNA and DNA that do most of the work of the cell are typically far smaller than 0.2 micrometers, and to visualize them, microscopists have struggled to overcome the diffraction limit. They have developed several clever methods that accomplish this, but some of them require special microscopes that tend to be very expensive, and others require cumbersome procedures. What's more, today's methods can only reveal a handful of distinct molecule species at a time, and the images remain blurrier than many scientists would like.
The Wyss Institute-led team plans to overcome these challenges by combining single-molecule imaging methods with molecular tools from DNA nanotechnology. Using an imaging method called DNA-PAINT, they created so-called "imager strands" by tagging small pieces of DNA with a fluorescent dye. Each of these imager strands binds transiently to a matching DNA strand that is attached to a target molecule, which makes the target appear to blink. Such blinking, when done right, enables scientists to beat the diffraction limit and obtain sharper images of the targets than otherwise possible.
"The powerful thing about using DNA lies in its amazing programmability," Yin said. "We plan to use that capability to make molecules in cells blink in a programmable and autonomous way. This will allow us to see things that were previously invisible."
Go here to see the original:
DNA nanotechnology opens new path to super-high-resolution molecular imaging
- Electronic Materials Company Cambrios Announces Closing $14.5 Million Round - November 8th, 2009 [November 8th, 2009]
- Europe's 'Extreme light infrastructure' project gears up for launch - November 8th, 2009 [November 8th, 2009]
- Industrial nanotechnology processes getting closer - November 8th, 2009 [November 8th, 2009]
- Konsultation zum Austria Nano-Aktionsplan - November 8th, 2009 [November 8th, 2009]
- Bewegungen von Viren auf Membranen live verfolgen - November 8th, 2009 [November 8th, 2009]
- Thailand Nanotechnology Conference on health, energy, environment - November 8th, 2009 [November 8th, 2009]
- Samsung Intensifies Advanced Foundry Logic Process Development with New Semiconductor Research Center - November 8th, 2009 [November 8th, 2009]
- Infineon and TSMC to Jointly Develop 65nm Embedded Flash Process Technology For Automotive and Chip Card Applications - November 8th, 2009 [November 8th, 2009]
- Abraxis Health Dedicates State of the Art Nanotechnology and Biologics Facility in Phoenix, Arizona - November 8th, 2009 [November 8th, 2009]
- Beneq Announces The First Continous Mode Atomic Layer Deposition Research Tool - November 8th, 2009 [November 8th, 2009]
- An electron microscope with glasses - November 8th, 2009 [November 8th, 2009]
- Give your opinion on the Nanotechnology Research Code of Conduct - November 8th, 2009 [November 8th, 2009]
- Presentations from the OECD Conference on the Potential Environmental Benefits of Nanotechnology - November 8th, 2009 [November 8th, 2009]
- DFI Nanotechnology Expands Into China With Strategic Alliance in Hong Kong - November 8th, 2009 [November 8th, 2009]
- Pre-announcement: Call for proposals within 'Energy Efficiency with Nanotechnology' - November 8th, 2009 [November 8th, 2009]
- This week in nanotechnology - November 8th, 2009 [November 8th, 2009]
- New funding for development and deployment of UAlbany nanofabrication strategies - November 8th, 2009 [November 8th, 2009]
- Applied Materials Acquires the Assets of Advent Solar - November 8th, 2009 [November 8th, 2009]
- Advanced Researcher Grant 2009 an Nanowissenschaftlerin Luisa de Cola - November 8th, 2009 [November 8th, 2009]
- Ocean Optics Launches Dedicated OEM Website - November 8th, 2009 [November 8th, 2009]
- New synthetic molecules trigger immune response to HIV and prostate cancer - November 8th, 2009 [November 8th, 2009]
- Computer predicts reactions between molecules and surfaces, with 'chemical precision' - November 8th, 2009 [November 8th, 2009]
- Austria showcases its nanotechnology initiative - November 8th, 2009 [November 8th, 2009]
- Nanocapsules for artificial photosynthesis - November 8th, 2009 [November 8th, 2009]
- Work begins on laboratory for world's strongest microscope - November 8th, 2009 [November 8th, 2009]
- New cancer detection method with fluorescent silica beads - November 8th, 2009 [November 8th, 2009]
- Die Magnetisierung einzelner Atome manipulieren - November 8th, 2009 [November 8th, 2009]
- Novel nanoparticles could become a safer alternative to gene therapy delivered by viruses - November 8th, 2009 [November 8th, 2009]
- DOE awards Argonne, partners more than $7 million for solar energy-related research projects - November 8th, 2009 [November 8th, 2009]
- Magnetic nanoparticles to simultaneously diagnose, monitor and treat - November 8th, 2009 [November 8th, 2009]
- Study of ultracold atoms proves theory about universal quantum mechanism - December 13th, 2009 [December 13th, 2009]
- RUSNANO Supervisory Council approves nanostructured non-metallic coatings project - December 13th, 2009 [December 13th, 2009]
- Julian Wess Award for outstanding achievements in elementary particle and astroparticle physics - December 13th, 2009 [December 13th, 2009]
- New Pegasus300 Sold to Leading French Research Institute for CMOS Packaging - December 13th, 2009 [December 13th, 2009]
- Soitec Expands Into the Fast Growing Solar Energy Market With the Acquisition of Concentrix Solar - December 13th, 2009 [December 13th, 2009]
- Students send microbe nanobiotechnology experiment on Space Shuttle Atlantis - December 13th, 2009 [December 13th, 2009]
- Photovoltaic cells to power biological nanorobots inside the body - December 13th, 2009 [December 13th, 2009]
- Environmental and human health impacts of nanotechnology - December 13th, 2009 [December 13th, 2009]
- Elusive 'hot' electrons captured in ultra-thin solar cells - December 13th, 2009 [December 13th, 2009]
- Light-generating transistors to power labs on chips - December 13th, 2009 [December 13th, 2009]
- Combining rare-earth clusters with traditional metal catalysts reveals secrets of chemical transformation - December 13th, 2009 [December 13th, 2009]
- Hexagon Expands Its Offering in the Vision Metrology Business - December 13th, 2009 [December 13th, 2009]
- Azaya Therapeutics Receives FDA Authorization to Start Phase I Cancer Trial - December 13th, 2009 [December 13th, 2009]
- Researchers engineer bacteria to turn carbon dioxide into liquid fuel - December 13th, 2009 [December 13th, 2009]
- Argonne creates green home for world-class nanotechnology research - December 13th, 2009 [December 13th, 2009]
- The mechanism behind superinsulation may lead to new types of electronics - December 13th, 2009 [December 13th, 2009]
- Biosensors change color when they detect specific neural messages - December 14th, 2009 [December 14th, 2009]
- First measurement of cancer biomarkers in whole blood - December 14th, 2009 [December 14th, 2009]
- Nanoprobes hit targets in tumors, could lessen chemo side effects - December 14th, 2009 [December 14th, 2009]
- EYP/energy group joins UAlbany NanoCollege's National Institute for Sustainable Energy - December 14th, 2009 [December 14th, 2009]
- Scientists isolate new antifreeze molecule in Alaska beetle - December 14th, 2009 [December 14th, 2009]
- Ocean Optics XR-Series Spectrometers Cover 200-1050 nm Wavelengths - December 14th, 2009 [December 14th, 2009]
- All-in-one cancer-killing nanoparticle can be tracked in real time with MRI - December 14th, 2009 [December 14th, 2009]
- Nanobodies modifizieren die Form und Funktion von Proteinen - December 14th, 2009 [December 14th, 2009]
- Elias A. Zerhouni, M.D. to Join Leica Microsystems' Parent Company Board of Directors - December 14th, 2009 [December 14th, 2009]
- France to invest billions in nanotechnology - December 14th, 2009 [December 14th, 2009]
- AlphaRx Appoints President for its China Operations - December 14th, 2009 [December 14th, 2009]
- Vistec Enters Into Electron-Beam Lithograpy Project With Moscow University - December 14th, 2009 [December 14th, 2009]
- Record-breaking ultrasensitive spectrometer identifies trace gases in real time - December 14th, 2009 [December 14th, 2009]
- NanoMech Receives Navy Contract to Develop Nanotechnology Lubricant - December 14th, 2009 [December 14th, 2009]
- Heart cells display a behavior-guiding 'nanosense' on new lab-on-a-chip - December 14th, 2009 [December 14th, 2009]
- New curriculum mixes nanotechnology and skiing - December 14th, 2009 [December 14th, 2009]
- Rexahn and TheraTarget Form Nanotechnology Research Collaboration - December 14th, 2009 [December 14th, 2009]
- Unidym Enters Agreement to Integrate Carbon Nanotube Films Into LCDs - December 14th, 2009 [December 14th, 2009]
- Carl Zeiss Meditec Closes Financial Year on a High - December 14th, 2009 [December 14th, 2009]
- The Scott Partnership Appointed to Launch Innovative New Printed Electronics Business - December 14th, 2009 [December 14th, 2009]
- New Centre for Molecular Epidemiology to put bacteria on the world map - December 14th, 2009 [December 14th, 2009]
- New nanotechnology association established to address 21st century natural resource and energy security challenges - December 14th, 2009 [December 14th, 2009]
- Analysis of information gathering initiatives on manufactured nanomaterials - December 14th, 2009 [December 14th, 2009]
- OECD publishes manufactured nanomaterials roadmap 2010 - December 14th, 2009 [December 14th, 2009]
- New imaging technique allows quick evaluation of graphene sheets - December 14th, 2009 [December 14th, 2009]
- National Institute of Standards and Technology invests up to $71 million in new manufacturing, infrastructure research and development - December 16th, 2009 [December 16th, 2009]
- Southwest Nanotechnologies Recieves Two Prestigious Research Grants To Deveop A New Generation of Carbon Nanotubes - December 16th, 2009 [December 16th, 2009]
- Nanolithographic technique allows multiple chemicals on a single chip - December 16th, 2009 [December 16th, 2009]
- Study documents puzzling movement of electricity-producing bacteria near energy sources - December 16th, 2009 [December 16th, 2009]
- Erstmals Daten aus lebenden Zellen: Optisches Verfahren misst molekulare Reaktionszeiten - December 16th, 2009 [December 16th, 2009]
- New Constant Diameter NeedleProbes with Unique Ability to Measure Liquid Surface Properties by AFM - December 16th, 2009 [December 16th, 2009]
- Chemical Nanotechnology Talks X: Green nano - challenges of sustainability - December 16th, 2009 [December 16th, 2009]
- Patents Issued for Licensed PAL-M Super-resolution Technology - December 16th, 2009 [December 16th, 2009]
- International Network CONTACT researches the use of carbon nanotubes in various applications - December 16th, 2009 [December 16th, 2009]