Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. Especially gold nanoparticles are of increasing interest for nanobiotechnology research and applications because of their high acceptance level in living systems and the fact that they are fairly easily conjugated with functional molecules. Ultrashort pulsed laser ablation represents a powerful tool for the generation of pure gold nanoparticles avoiding chemical precursors, reducing agents, and stabilizing ligands. The bare surface of the charged nanoparticles makes them highly available for functionalization and as a result especially interesting for biomedical applications. Starting today, such conjugates are available commercially for the first time.