Measuring the force of a single synthetic small molecule

Previously, synthetic molecular machines have been used to perform mechanical tasks collectively, such as move liquid droplets uphill against the force of gravity, rotate microscale objects using liquid crystals doped with synthetic motor-molecules, and bend cantilevers. However, all these tasks are achieved by the collective action of billions and billions of molecular machines. Observing the mechanical behavior of an individual molecule is much more difficult. Synthetic molecular machines are often ten times smaller in each dimension than motor proteins and previously no one has managed to use single molecule techniques to look at how the components move in synthetic molecular machines. By using very sensitive atomic force microscopy experiments, researchers now were able to address the movement of the ring in individual rotaxane molecules.

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