The Zacks Analyst Blog Highlights: NetEase, New Oriental Education & Technology and JinkoSolar – Nasdaq

For Immediate Release

Chicago, IL November 14, 2019 Zacks.com announces the list of stocks featured in the Analyst Blog. Every day the Zacks Equity Research analysts discuss the latest news and events impacting stocks and the financial markets. Stocks recently featured in the blog include: NetEase, Inc. NTES, New Oriental Education & Technology Group Inc. EDU and JinkoSolar Holding Co., Ltd. JKS.

Here are highlights from Wednesdays Analyst Blog:

3 Chinese ADRs to Gain from a Likely Trade Deal

A trade agreement between the United States and China is still uncertain despite encouraging comments from officials of both nations. However, the recent enthusiasm of the two nations in reaching a deal made investors somewhat optimistic lately.

There has been a decent progress on the trade deal front in the past few weeks. Particularly, comments by President Donald Trump and economic advisor Larry Kudlow earlier this week hinted at a potential trade deal.

Let us, thus, take a look at some U.S.-listed Chinese stocks that will benefit the most if the a deal materializes.

Rising Optimism on U.S.-China Trade Relationship

The U.S.-China trade war has rattled the financial markets for 19 months, as industries reliant on trade suffered because of the high tariffs that the two countries imposed on each others imports. However, there has been some relief since October 2019 after the two countries agreed on a phase one deal.

Two major developments emerged out of the October trade talks. First, Trump decided not to proceed with an escalation in tariffs on about $250 billion worth of Chinese goods from the incumbent 25% to 30% beginning Oct 15. Second, a 15% tariff imposition on about $156 billion of Chinese products (known as List 4B) from Dec 15 was also kept on hold.

While speaking at the Economic Club of New York on Nov 12, Trump blamed previous U.S. leaders who negotiated trade deals with China with scope for manipulation. However, the president repeated that a significant phase-one trade deal with the China could be reached.

Addressing the issue of cancelling punitive tariffs, Trump said, China would like to get somewhat of a rollback, not a complete rollback, because they know I won't do itFrankly, they want to make a deal a lot more than I do.

Trumps comments aptly point toward the trade wars adverse effect on China. Chinas factory activity shrank for the sixth straight month in October, as the Purchasing Managers Index for manufacturing came in at 49.3. A reading below 50 indicates contraction.

Later on the same day, Kudlow said in an interview that there is a possibility of tariffs to be adjusted, but there would be no adjustment until a final deal is reached. Kudlows comments were in response to Chinas demand for the existing tariffs to be removed as part of the phase one trade deal.

He said that the two countries had made progress on issues such as intellectual property theft, financial services, currency stability, commodities and agriculture. Kudlow added that there could be some issues as part of a second-phase agreement with China.

Also, the agreement, which was anticipated to be signed at the APEC Summit in November, has been deferred to December.

Our Choices

We have, therefore, shortlisted three U.S.-listed Chinese stocks that have outperformed the Zacks S&P 500 composite so far this year and are well positioned to do even better if a final deal between the two countries is reached. After all, business growth of these companies will accelerate if the trade barriers are removed.

These three stocks carry a Zacks Rank #1 (Strong Buy) or 2 (Buy).

NetEase, Inc. is an operator of an interactive online community in China. The company offers online gaming services, e-commerce and advertising services etc. The company carries a Zacks Rank #1. The Zacks Consensus Estimate for Coherus BioSciences current-year earnings has risen 1.1% over the past 60 days. The stock has gained 25.9% year to date versus the S&P 500s 21.9% growth. You can seethe complete list of todays Zacks #1 Rank stocks here.

New Oriental Education & Technology Group Inc.is a provider of private educational services in China. The company offers language training and test preparation courses, online education programs and overseas studies consulting services etc. The company carries a zacks Rank #1. The Zacks Consensus Estimate for Coherus BioSciences current-year earnings has risen 0.3% over the past 60 days. The stock has gained 118.4% year to date.

JinkoSolar Holding Co., Ltd.is a designer, manufacturer and marketer of photovoltaic products. The company carries a Zacks Rank #2. The Zacks Consensus Estimate for Coherus BioSciences current-year earnings has risen 7.5% over the past 60 days. The stock has gained 62.3% year to date.

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Past performance is no guarantee of future results. Inherent in any investment is the potential for loss. This material is being provided for informational purposes only and nothing herein constitutes investment, legal, accounting or tax advice, or a recommendation to buy, sell or hold a security. No recommendation or advice is being given as to whether any investment is suitable for a particular investor. It should not be assumedthat any investments in securities, companies, sectors or markets identified and described were or will be profitable. All information is current as of the date of herein andis subject to change without notice. Any views or opinions expressed may not reflect those of the firm as a whole. Zacks Investment Research does not engage in investment banking, market making or asset management activities of any securities. These returns are from hypothetical portfolios consisting of stocks with Zacks Rank = 1 that were rebalanced monthly with zero transaction costs. These are not the returns of actual portfolios of stocks. The S&P 500 is an unmanaged index. Visit http://www.zacks.com/performancefor information about the performance numbers displayed in this press release.

NetEase, Inc. (NTES): Free Stock Analysis Report

New Oriental Education & Technology Group, Inc. (EDU): Free Stock Analysis Report

JinkoSolar Holding Company Limited (JKS): Free Stock Analysis Report

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The Zacks Analyst Blog Highlights: NetEase, New Oriental Education & Technology and JinkoSolar - Nasdaq

There Will Be No Turning Back on Facial Recognition – New York Magazine

Taylor Swift has used facial recognition at concerts to screen for stalkers. Photo: Gareth Cattermole/TAS18/Getty Images for TAS/2018 Gareth Cattermole/TAS18

On Friday, August 16, at around 7 a.m., a pair of suspicious appliances was found on a subway platform at the Fulton Street station in lower Manhattan and, an hour later, a third near a garbage can on West 16th Street. Initially, police thought they might be improvised bombs, like the shrapnel-filled pressure cookers that blew up at the 2013 Boston Marathon and in Chelsea in 2016, but upon inspection they turned out to be harmless empty rice cookers, probably meant to scare but not explode. Trains were delayed during the morning commute, but since that happens often enough without any terrorist help at all, the scariest thing about this episode may have been the way the alleged perpetrator was caught.

Minutes after the discovery, the NYPD pulled images of a man leaving the devices from subway surveillance cameras and gave them to its Facial Identification Section (FIS), which ran them through software that automatically compared his face to millions of mug shots in the police departments database. The program spit back hundreds of potential matches in which officers quickly spotted their person of interest: Larry Griffin II, a homeless 26-year-old the NYPD had arrested in March with drug paraphernalia. FIS double-checked its surveillance pictures against Griffins social-media accounts, and by 8:15 a.m., his name and photos were sent to the cell phones of every cop in New York. He was arrested in the Bronx late that night and charged with three counts of planting a false bomb. (He pleaded not guilty.)

This might seem like a feel-good story: A potentially dangerous person was identified and apprehended with previously impossible speed and no casualties thanks to by-the-book use of new technology (or newish; the NYPD has used facial-recognition software since 2011). But zoom out a little and it looks more like a silver lining on one of this years biggest feel-bad stories: The facial-recognition system that ensnared Griffin is only a small piece of a sprawling, invisible, privacy-wrecking surveillance apparatus that now surrounds all of us, built under our noses (and using our noses) by tech companies, law enforcement, commercial interests, and a secretive array of data brokers and other third parties.

In 2019, facial recognition may have finally graduated from dystopian underdog it was only the fourth- or fifth-most-frightening thing in Minority Report; its never played more than a supporting role on Black Mirror; and in the Terminator movies, it was a crucial safety feature preventing the Terminator from terminating the wrong people to full-grown modern worry.

A brief recap of the year of Face Panic:

This spring, we heard that the FBIs facial recognition database now includes more than 641 million images and the identities of an unsuspecting majority of Americans, which can be searched anytime without warrant or probable cause.

We also heard that spooked lawmakers banned police use of facial recognition in Oakland; Berkeley; Somerville, Massachusetts; and San Francisco, of all places, where Orwellian tech products are the hometown industry.

But everywhere else and in all other contexts, facial recognition is legal and almost completely unregulated and we heard that its already being used on us in city streets, airports, retail stores, restaurants, hotels, sporting events, churches, and presumably lots of other places we just dont know about.

Over the summer, we heard a rumor about a novelty photo app that might be a secret ploy by the Russian government to harvest our selfies for its own facial recognition database. That turned out to be false (or at least we think so), but it mightve made us reconsider all the photos weve given to other companies whose loophole-filled terms of use agreements translate to we own your face including Facebook, which is thought to have one of the worlds largest facial recognition databases and has not exactly proven itself to be the most responsible custodian of our private information.

We barely heard about the Commercial Facial Recognition Privacy Act of 2019, a bill introduced in March by Republican Senator Roy Blunt and Democrat Brian Schatz that would have prohibited companies from using facial recognition to track you in public, and collect or sell your face data, without your consent. It was the kind of common-sense bipartisan proposal that almost everybody can agree with; it was referred to a committee and never made it to a vote.

But we heard a lot about facial recognitionsunreliability, and that misidentifications are common, especially for people of color, and that the London Metropolitan Polices system has an appalling failure rate, and that Amazons software once mistook a bunch of seemingly upstanding congresspeople for criminal suspects. Maybe that meant, we hoped, that the technology was still only half-baked and that our worries were premature, and that one big lawsuit could make it all go away.

And then we heard about Larry Griffin II. And if his story was meant to calm any fears about facial recognition, well, mission not necessarily accomplished. If the software works well enough to identify one bomb-hoax suspect among 8.4 million New Yorkers in under an hour using only a couple of grainy surveillance photos, maybe our worries arent so premature after all. Because even though facial recognition isnt fully reliable yet, and it might never be, its already transforming law enforcement. And it probably wont need to be perfect before it transforms our everyday lives, too.

In a possibly related story, perhaps you have noticed an unusually high demand for pictures of your face over the past several years?

Computerized facial recognition has been in development since the 1960s. Progress was steady but slow, though, until the recent arrival of advanced artificial neural networks i.e., computer systems modeled on animal brains that can recognize patterns by processing examples which have allowed human programmers to feed these networks many photos of faces and then step aside while algorithms teach themselves what faces look like and then how to tell those faces apart and eventually how to decide if a face in one photo is the same face in a different photo, even if that face is wearing sunglasses or makeup or a mustache or is poorly lit or slightly blurry in one image but not the other. The more photos an algorithm has to learn from millions or billions, ideally the more accurate it becomes.

Some companies asked you to upload your vacation photos and tag yourself in them. And others asked for a selfie in exchange for an autogenerated cartoon caricature of you or to tell you which celebrity or Renaissance painting you most resemble. And then some companies got impatient with all this asking, so they acquired the other companies that had already collected your photos, or they scraped public images of you from social media and dating sites, or they set up hidden cameras in public spaces to take their own pictures.

How many algorithms have been trained on your face? Hard to say, because there arent really any laws requiring your consent, but Facebook, Google, Amazon, Apple, Microsoft, IBM, and dozens of start-ups with names like Facefirst, FaceX, and Trueface all have their own facial-recognition algorithms, and they had to have learned somewhere.

Some of those algorithms are pretty accurate, too, at least under optimal conditions. Last year, the U.S. Department of Commerces National Institute of Standards and Technology (NIST) tested 127 facial-recognition algorithms from 40 developers to see how often they could find matches in a large database. With high-quality images to compare, the top-performing algorithms only failed to return the correct match in 0.2 percent of searches, which is 20 times better than the results of a similar test in 2014.

Facial recognition will inevitably improve on its own, but it might be even more accurate when used in combination with other long-range biometrics. In China, gait recognition is already identifying people based on the way they walk, with 94 percent accuracy according to one company offering the service. And the Pentagon claims to have developed a heartbeat laser that is, an infrared beam that can read a persons unique cardiac signature through a shirt or jacket, allegedly with 95 percent accuracy. (The current model can reportedly detect a heartbeat from 200 meters away, but with a slightly better laser, however: I dont want to say you could do it from space, a Pentagon source told the MIT Technology Review, but longer ranges should be possible.)

For facial-recognition software to recognize you in the wild, it needs to be connected to a database with your photo and identifying information in it. (Just because an algorithm has trained with your face doesnt mean it knows who you are.) Unless youre Elena Ferrante or a member of Daft Punk, and maybe even if you are them, theres a good chance youre in a database or two.

If youve ever been tagged in a photo on Facebook or Instagram, for example, you belong to what is by Facebooks own claims the worlds largest facial-recognition database, to which users add hundreds of millions of new images every day. The companys facial-recognition algorithm, DeepFace, which is constantly retraining itself on those new images, is presumed to be more accurate than software used by most law-enforcement agencies.

For now, Facebook says it only uses DeepFace to suggest tags when users upload new pictures. But that didnt help the company in August when a federal appeals court ruled that 7 million Facebook users in Illinois can sue the company for storing their face data without permission, which they claim is a violation of the states BiometricInformationPrivacy Act, currently the only such law in the U.S., which could make Facebook liable for as much as $5,000 per affected user or $35 billion total. (In response, Facebook has stopped suggesting tags for photos by default.)

The FBIs facial-recognition database spans mug shots, the State Departments entire directory of visa and passport pictures, and photos from the Departments of Motor Vehicles in at least 22 states (and counting) that allowed the agency to scan residents drivers licenses without their consent. (In Utah, Vermont, and Washington, where undocumented immigrants can legally drive, DMVs have shared facial recognition data with Immigration and Customs Enforcement.) If youve managed to keep yourself out of the FBIs database so far, that may get harder in October 2020, when Americans in 47 states will be required to show either a passport or a new federally compliant Real ID drivers license to board any domestic flight.

All of this would be bothersome enough in a world with perfect data security or one in which you could get a new face as easily as your bank replaces a stolen debit card. But we live in neither of those worlds, and faces have already been hacked. In June, U.S. Customs and Border Protection announced that hackers had breached the servers of one of its subcontractors and stolen travelers face data, some of which reportedly turned up on the dark web.

Despite plenty of more deserving scandals, though, none caused as much hysteria in 2019 as FaceApp. In July, all of your friends, plus Snooki, the Jonas Brothers, and Lebron James, shared pictures taken with the image-processing tool, which went viral by making users look like elderly people. But panic ensued when they read FaceApps merciless terms of use agreement. (You grant FaceApp a perpetual, irrevocable, nonexclusive, royalty-free, worldwide, fully-paid, transferable sub-licensable license to use, reproduce, modify, adapt, publish, translate, create derivative works from, distribute, publicly perform and display your User Content and any name, username or likeness provided in connection with your User Content in all media formats and channels now known or later developed, without compensation to you.) And then even more panic ensued when they found out FaceApp is headquartered in St. Petersburg, Russia. Had Vladimir Putin tricked America again, this time into handing over the elusive face data of its influencers?

No, or at least probably not. FaceApp denied that the company shares data with third parties, and claimed most user images are deleted within 48 hours, which seemed to stem fears. But if FaceApp did decide to pull up stakes and pivot to facial recognition, it would not be the first company like it to do so: The photo-management app EverRoll launched in 2012 and collected 13 billion images in which users had tagged themselves and their friends. EverRolls parent company, which recently renamed itself Paravision, discontinued the app in 2016 and used those images to train its facial recognition software, which is now ranked as the No. 3 most accurate algorithm tested by NIST and is sold to law enforcement.

The worst-casescenario for facial recognition might look like something likeChinas forthcoming social credit system.When the system is fully operational next year, the government will use all surveillance methods at its disposal, including facial recognition and 200 million cameras, to track citizens behavior and assign each of them a social score, which will have a variety of consequences. Infractions such as jaywalking and buying too many video games could make it harder to rent an apartment or get a loan from a bank. That probably isnt likely in the U.S., but a more ordinary kind of surveillance is almost inevitable. Maybe its already here.

Commercial facial recognition has been around for years, but since there arent any laws requiring anyone to disclose that theyre using it on you, its impossible to say how widespread it is. Which means any camera you pass could be recognizing your face.

Among the many vendors offering commercial facial recognition software is Face-Six, a Tel Avivbased company founded by Moshe Greenshpan in 2012 after Skakash, a mobile app he was developing that would have identified actors in movies and TV shows, failed to attract investors. Greenshpan says he now serves more than 500 customers worldwide, offering a variety of custom products, including FA6 Retail (to prevent shoplifting), FA6 Class (to take attendance in schools), FA6 Med (for use in hospitals to verify patients identities and prevent treatment errors), and FA6 Drone (which identifies criminals, missing people, and civilians from a drones camera, and which is available for government and private uses.) But Greenshpans most controversial product has been Churchix, which he sells to churches that want to keep track of which parishioners are showing up to mass.

Churchix attracted negative coverage when it launched in 2015, some of which is linked to on the companys website. In the beginning, all press was good press, says Greenshpan. You really need to explain to people why facial recognition is more good than bad. Churches manage attendance manually, but when we provide an efficient tool that does it automatically, suddenly there are concerns. And if those concerns include transparency? We dont know what each and every customer does with our software, Greenshpan says, but usually churches [that use Churchix] dont tell their members.

But Greenshpan does connect me with David Weil, founder of the Warehouse, an after-school recreation center and skateboarding park in Bloomington, Indiana, which uses Face-Sixs software on two security cameras, for one extremely specific purpose: Our building is five acres under one roof, and there are over 200 registered sexual offenders in the area, says Weil. So with that much space and that many pedophiles, this software really helped us.

As visitors enter the building, the cameras scan their faces, and Face-Sixs software searches them against a database provided by the local sheriffs office. According to Weil, the Warehouse had 72,000 visits in 2018, and facial recognition managed to keep out two sex offenders: We just politely told them, Were a youth center. Youre not allowed to be here, he says. I had one false ID, but the gentleman was very understanding of the situation.

Among the other ways we know facial recognition is already being deployed:

Airlines are using it to replace boarding passes, and the Department of Homeland Security says it plans to use facial recognition on 97 percent of airline passengers by 2022.

And at least three arenas have experimented with the technology, including Madison Square Garden.

On stops of Taylor Swifts recent Reputation tour, fans faces were scanned and searched against a database of her hundreds of known stalkers.

Last year, residents of an apartment complex in Brownsville protested when they found out their landlord wanted to use facial recognition to supplement their key fobs but other buildings in the city have been known to use it for years, including the 12 that make up the Knickerbocker Village complex on the Lower East Side. Some virtual-doorman systems include it, too.

At least eight public-school districts in the U.S. have installed facial-recognition systems to detect suspended students and anyone else banned from school grounds.

Retailers use facial recognition to prevent theft, and some software even comes bundled with databases of known shoplifters, but not many stores will admit to it. Last year, the ACLU asked 20 top retail chains whether they use facial recognition, including Best Buy, Costco, Target, and Walmart, and only received two answers rom the grocery conglomerate Ahold Delhaize, whose brands include Food Lion and Stop & Shop, which doesnt use it, andfrom the hardware chain Lowes, which tested facial recognition in the past but has since stopped.

Retailers dont need to run facial-recognition software on their premises to benefit from it. Apple denies using the technology in its brick-and-mortar locations, but in one confusing incident last year, a New York teenager was arrested and charged with stealing from multiple Apple Stores when police said he was identified by facial recognition. Apple apparently employs an outside firm called Security Industry Specialists in some locations; SIS may have run facial-recognition software on surveillance footage captured inside the Apple Stores the teen was alleged to have stolen from. (Charges were dropped against him when an NYPD detective realized he looked nothing like the suspect in the footage of the robberies. In April, the teen sued Apple and SIS for $1 billion.)

Facial recognition could soon be more valuable to retailers in other ways. One likely possibility is that some will eliminate checkout lines by having customers pay with their faces. Another is using facial recognition to target the people most likely to buy things by tracking their in-person shopping habits the same way cookies track our online ones or maybe, eventually, using what they know about our virtual selves to direct us toward products in the real world. If youve ever been creeped out by an uncannily well-targeted ad served to you on Facebook or Instagram, imagine being helped by a retail employee who knows whats in your web history.

But what if youre not the person facial recognition says you are? Last year, the ACLU used Amazons facial recognition software, Rekognition, to search a database of 25,000 mug shots using photos of members of Congress and found that it misidentified 28 lawmakers as criminals, including six members of the Congressional Black Caucus, and a similar test this summer mistook 26 California state legislators, again with people of color overrepresented in the false matches.

Amazon says the ACLU intentionally misrepresented its software by setting its confidence threshold too low the company recommends that police act only on matches in which the system expresses at least 99 percent confidence but theres nothing to prevent police from doing the same thing. Its toothless, says Jacob Snow, the technology-and-civil-liberties attorney at the ACLU who ran the tests. Amazon could say to law enforcement, Were going to set the confidence threshold at 99 percent, and you cant change it. But theyre not doing that.

Dark skin isnt the only thing facial recognition fails with. In NISTs tests, it found that even top-performing algorithms had trouble identifying photos of the same person at different ages and were often unable to tell the difference between twins not just identical twins but fraternal ones of the same sex, too. And performance depends on the clarity of the photos being used. NIST was primarily comparing high-quality mug shots to other high-quality mug shots, but under real-world conditions, with blurry surveillance photos taken at bad angles by cameras that may have been set up incorrectly, results may vary.

Even a facial-recognition system with low error rates can cause problems when deployed at large scale. During six recent tests of the London polices facial-recognition system, which scanned the faces of people on public streets in search of wanted suspects, 42 matches were made but only eight were verified to be correct (30 matches were eventually confirmed to be misidentifications, and four of the 42 people disappeared into crowds before officers were able to make contact). Because they scanned thousands of faces in total, the London police said their error rate was 0.1 percent, but most headlines begged to differ: LONDON POLICE FACIAL RECOGNITION FAILS 80% OF THE TIME AND MUST STOP NOW, said one.

Police have also been caught taking creative license with the technology. A report published in May by the Georgetown Universitys Center on Privacy and Technology found that six departments in the U.S. allow officers to run composite sketches of suspects through facial-recognition software. That same report tells the story of a suspect whod been caught by surveillance cameras allegedly stealing beer from a CVS in Gramercy Park in 2017, but the video was low quality and no useful matches were returned. A detective noticed, though, that the man bore a resemblance to Woody Harrelson, so he ran a search using an image of the actor which eventually led to an arrest. The person they ended up investigating was the tenth person on the candidate list, says Georgetown Laws Clare Garvie, the author of the report, meaning the algorithm thought nine other people [in the NYPDs mug-shot database] looked more like Woody Harrelson. If you can put person A into an algorithm and find person B, why does that not prove that if youre looking for looking for person B, you might accidentally find person A? They intentionally forced a misidentification as a valid investigative technique.

NYPD spokesperson Devora Kaye notes that this case was just one of more than 5,300 requests to FIS in 2017. The NYPD, she says, only uses facial recognition as an investigative tool and doesnt arrest or detain suspects whose identities havent been corroborated by other means. A facial recognition match is a lead. No one has ever been arrested solely on the basis of a computer match, no matter how compelling.

If Larry Griffin IIs story typifies a best-case use of facial recognition for law enforcement, Kaitlin Jackson, a public defense attorney with the Bronx Defenders, tells me one that exposes its drawbacks. Jackson represented a man whod been arrested for the theft of socks from a T.J. Maxx store in February 2018, supposedly after brandishing a box cutter at a security guard. My client was picked up months after the robbery, and the only way I even found out facial recognition was used was that I just started calling the prosecutor and saying, How in the world did you decide months after that it was my client? There are no forensics, she says. It turned out the police went to T.J. Maxx security and said, We want to pull the surveillance , were going to run it through facial recognition so they were already cluing him in that any suspect will have been picked by facial recognition. And then they texted the security guard a single photo that he knows has been run through facial recognition, and they said, Is this the person? Thats the most suggestive procedure you could possibly imagine. And then they make the arrest and say its on the basis of an [eyewitness] ID, and they try to bury that this is a facial-recognition case. (The NYPD said the defendant had committed a theft at the same store before and said the security guard knew him from prior interactions. The detective on the case showed him an image hoping it would put a name to a face, the department said in a legal filing.)

Jacksons client had at least two lines of defense: He has a twin brother, who could have triggered the facial-recognition match although Jackson doesnt think the twin stole any socks either but more important, his wife was in labor at the time of the theft and he was in the delivery room. We had pictures of them at the hospital, and his name was on the birth certificate, says Jackson. But the prosecution would not dismiss the case partly, she suspects, because they have an undying faith that the software doesnt get it wrong. Their only recourse, she said, would be to argue, Maybe he left a few minutes before his baby was delivered and ran out to get socks and then came back.

Jackson says her client spent half of last year in prison. He was on probation when he was arrested. So our real problem was the way that all these systems interact. Probation lodged a hold, and they would not withdraw the hold because of this case, and the prosecution wouldnt dismiss this case. And then finally [the prosecution] offered him something that would get him out of jail. So he did what a lot of us would he took a plea of something he did not do.

But facial recognition is about more than just who you are, and what youve bought, and the crimes you have or havent committed, and whether your resemblance to a sex offender will make it hard to find places to skateboard its also about how you feel. Because another thing the technology can do, or at least supposedly do, is detect emotions.

Amazon, IBM, Microsoft, and others claim their software can guess which emotion youre feeling based on your facial expressions or in some cases microexpressions, under the logic that even if you attempt to hide your feelings, certain facial muscles are beyond your control.Amazon, for example, says Rekognition can infer whether a face is expressing happiness, sadness, anger, confusion, disgust, surprise, calmness, or fear although the documentation warns that results are not a determination of a persons internal emotional state and should not be used in such a way, and that a person pretending to have a sad face might not be sad emotionally.

Other vendors are more confident in their mood-reading abilities. The London-based start-up RealEyes markets a service to advertisers that scans peoples faces through their computers webcams to measure their attention level and emotions while they watch commercials. The Utah-based company Hirevue claims its software can analyze video job interviews to judge personality traits and eliminate disinterested candidates. (Hirevues clients include Dunkin Donuts, Staples, and Urban Outfitters.)

On its website, the facial-recognition company Kairos brags about the work it did for Legendary Pictures gauging reactions of audience members at movie test screenings: More than 450,000 emotional measurements were recorded per minute over the screening of preview films, for a total of around 100 million facial measurements processed in total. Kairos says Legendary used that data to determine which parts of movies worked best in ads, identify the demographics most likely to share trailers, and ensure Legendarys movies were appealing to mainstream audiences as well as their targeted fan demographic. Neither Kairos nor Legendary Pictures would confirm which movies were involved, but Kairos CEO Melissa Doval says the screenings probably happened in 2013 or 2014, and theres a picture from the 2013 Superman movieMan of Steelon Kairos website.

Your facial tics may not necessarily lose you any jobs or give the wrong impression about your taste in superhero movies, though because emotion-detection software might not really work. In July, a group of five top psychologists published areportinPsychological Science in the Public Interest,which cited over 1,000 other journal articles and found that facial expressions are more complex than the software gives them credit for: It is not possible to confidently infer happiness from a smile, anger from a scowl or sadness from a frown, as much of current technology tries to do when applying what are mistakenly believed to be the scientific facts.

Ultimately, the most worrisome thing about facial recognition might be how accessible it is. Because its not just available to governments and corporationsits also for sale to you, me, your landlord, random perverts, and anyone else with a camera and a computer, and for cheaper than youd probably expect: Amazons Rekognition, for example, offers a free year-long trial that lets you identify faces in 5,000 images or 1,000 minutes of video per month (and after that, its a penny per ten photos or ten cents per minute of video).

If none of the off-the-shelf software packages suit your particular needs, it turns out its pretty easy, if you know what youre doing, to roll your own facial-recognition app using freely available open-source code. I watched one YouTube tutorial in which a programmer built his own facial-recognition system that could distinguish between his face and the cast of Game of Thrones.

Its not hard to imagine more sinister applications. This spring, a developer claimed on Chinese social media that hed used facial recognition and publicly available photos from Facebook and Instagram to identify 100,000 women in amateur porn videos. He didnt share any proof and may have been lying but given all the other seemingly unbelievable things about facial recognition we now know to be true, maybe he wasnt.

I wanted to experiment myself, so I bought a Tend Insights Lynx Indoor 2, a tiny and cheap but well-reviewed home security camera that comes bundled with its own facial-recognition software. It worked well for what it did, which in my case wasnt much: I set the camera on my desk, connected it to my Wi-Fi network, downloaded the companion iPhone app, and uploaded a picture of myself so it knew what I looked like. I left my apartment, and in a few minutes when I came back, the Lynx sent a push notification to my phone to tell me I was home, along with a short video for proof. That may not sound like $60 well spent, but it was a small price for a feeling of control I might never have again: After the inaugural test of my home facial-recognition system, I unplugged it and stuffed it in a drawer.

*This article appears in the November 11, 2019, issue ofNew York Magazine. Subscribe Now!

The one story you shouldn't miss today, selected byNew York's editors.

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There Will Be No Turning Back on Facial Recognition - New York Magazine

USM’s Graham Participating in White House Summit on Partnerships in Ocean Science and Technology – Southern Miss Now

Thu, 11/14/2019 - 17:20pm | By: James Coll

Dr. Monty Graham, Associate Vice President for Research at The University of Southern Mississippi, Coastal Operations, is a participating in todays White House Summit on Partnerships in Ocean Science and Technology in Washington D.C.Graham is joining about 100 national leaders with ocean science expertise and interests in the nations capital for the summit hosted by Dr. Kelvin Droegemeier, Director of the Office of Science and Technology Policy, and Mary Neumayr, Chairman of the Council on Environmental Quality, within the Executive Office of the President. The summit is intended to bring together the private sector, academia, philanthropies, and the Federal government to discuss opportunities for building and sustaining partnerships in ocean-related research and promote technologies and investments.

The nation clearly has more needs than we have resources to meet those needs, Graham said. Partnerships are the key. What we are doing in the Gulf, in particular the Mississippi coast, is serving as a model to the nation on how to use partnerships between academia, industry, government and philanthropy to meet large and critical science and technology needs."

In addition to identifying opportunities to building cross-sector partnerships, the purpose of the summit is to engage the national community in the conversation through academic conferences and regional ocean science and technology centers. The event aligns with a 2018 Trump Administration executive order recognizing and supporting federal participation in regional ocean partnerships to advance the economic, security, and environmental interests of the United States.

Graham has also been recently selected to chair the national Consortium for Ocean Leadership (COL) Board of Trustees based in Washington, D.C. through 2021. He also serves as chair of Mississippi Governor Phil Bryants Ocean Task Force and co-chair of the Gulf Caribbean Oceanographic Consortium, and he is a board member for Ocean Exploration Trust, Inc.

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USM's Graham Participating in White House Summit on Partnerships in Ocean Science and Technology - Southern Miss Now

Virginia Beach Education Foundation using grant money for technology to improve physical education – 13newsnow.com WVEC

VIRGINIA BEACH, Va. It might not be the way you remember gym class.

The Virginia Beach Education Foundation is using $38,902.94 in grant money to transform physical education at two Virginia Beach locations.

The Juvenile Detention Center and Parkway Elementary School were surprised with grant money on Thursday.

The JDC received $18,802.94, which it'll use to buy "Heart Zone Systems." If you haven't heard of them, they're like FitBits. Students will wear them during PE.

The Juvenile Detention Center will use its grant money to buy Heart Zone Systems for students to wear during PE. Pictured, from left to right, Shelley Labiosa, VBCPS employee and grant reviewer; John Mason, math teacher; Superintendent Aaron Spence; Jon Mazach, Virginia Beach Education Foundation treasurer; Jeff McGowan, health and p.e. teacher; Charles Foster, principal; Janene Gorham, director of Professional Growth and Innovation for VBCPS.

Virginia Beach City Public Schools

Parkway students are getting a really cool upgrade.

The school is using its $20,000 grant to buy a "Lu Interactive Playground."

Parkway Elementary School P.E. teacher Casey Hughes was surprised with the grant check.

Virginia Beach City Public Schools

The technologically advanced playground includes light and audio systems and a giant wall projection with 3D cameras for interactive physical activity.

The wall projection can come with apps that focus on different subjects like math, geography, even problem solving among other things all while students stay active.

The grants are two of 79 "Adopt A+ Grants." The 79 grants total $200,000.

The foundation plans to continue to surprise teachers and schools across the city with the grant money.

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Virginia Beach Education Foundation using grant money for technology to improve physical education - 13newsnow.com WVEC

Common Networks bets 5G wireless technology will replace cable internet in your home – CNBC

A Common Networks field technician installing Terragraph technology on a roof in San Francisco

At first blush, inventing a start-up to challenge AT&T, Verizon and Comcast for high-speed broadband dominance seems crazy.

It takes billions of dollars to lay fiber in the ground historically, the only way to deliver ultrafast internet speeds to residential homes. The prohibitive cost has stopped some of the largest companies in the world from making a national dent in constructing high-speed broadband networks, including Verizon and Google. Meanwhile, Verizon and AT&T already plan to roll out 5G fixed broadband services in cities across the U.S., using wireless technology to compete with cable companies to provide home internet.

But Zach Brock, the CEO of Common Networks, a company he founded with three other ex-Square employees, said he believes he has helped design a technology that can upend the U.S. telecommunications market. For about $50 a month, Common Networks is offering 300Mb/sec to 1Gb/sec download speeds for households around Silicon Valley and Alameda (Oakland is coming soon). That's about $20 or $30 less per month than what Comcast charges for about the same speed without promotional pricing.

"Our focus is proving out of technology and showing we can deliver at an affordable cost," Brock said in an interview. "Our vision for the company is that everyone is connected to true broadband internet."

Common Networks, founded about three and a half years ago, leases existing fiber and uses unlicensed 5G microwave and millimeter spectrum to interact with its antenna-like devices that are installed on rooftops. Using open-source software and hardware, through a partnership with Facebook's Terragraph technology, Common Networks developed so-called graph-based technology that can deliver high-speed internet for what Brock said is about one-tenth the cost of what a telecommunications company has historically paid. To date Common Networks claims to reach more than 100,000 customers, though the company doesn't disclose its paying subscriber numbers.

The technology's prospects have been strong enough to bring in $34 million in funding from venture capital investors including General Catalyst, Eclipse Ventures and Lux Capital. On Tuesday the company made the 2019 CNBC's Upstart 100 list of the world's most promising start-ups.

Brock said his initial focus is single-family homes that don't have high-speed broadband options or have to rely on old DSL technology for Internet. Perhaps surprisingly, more than 22% of residents in San Leandro, California one of five cities Common Networks currently serves don't have a home broadband subscription of any kind. Cable companies often choose not to spend the money to connect buildings or regions of the country where they expect minimal customer retention.

"For many it may be likely that residential broadband internet is either not available or is not affordable," Magellan Advisors wrote in a review of the city's internet usage and capabilities.

More from Upstart 100:Amazon has triggered an arms race in this technologyWhy Kevin Durant and other NBA stars are betting millions on a start-up to challenge TV sportsAhead of 2020 presidential election, this start-up is using military-grade AI to stamp out fake news

Getting a foothold into homes that want broadband but can't access it may be the easiest path for the start-up, but the ultimate goal is to compete head-to-head against both cable companies and wireless providers, Brock said. Distributing broadband can be a very high-margin business, as most of the cost is fixed network infrastructure and upkeep. Cable companies, such as Comcast and Charter Communications, the two largest U.S. operators, have shifted their business models to invest billions of dollars in boosting nationwide speeds while spending less effort keeping traditional television subscribers.

Zach Brock, CEO of Common Networks.

Source: Common Networks

"Providing internet service is a very large market," said Kyle Doherty, a managing director at General Catalyst, whose venture capital firm led a $25 million Series B funding round in Common Networks last year. "High-speed broadband still isn't ubiquitous, but it will be in the future. There's a fair share of incumbents, but we're pretty compelled by the approach they're taking."

Common Networks doesn't lay any fiber nor does it need government permits to provide internet. Eschewing the enormous upfront costs of broadband infrastructure gives the company a chance to compete against much larger players if pricing is lower and customer service is dramatically better, said Doherty.

High-speed broadband still isn't ubiquitous, but it will be in the future. There's a fair share of incumbents, but we're pretty compelled by the approach they're taking.

Kyle Doherty

managing director, General Catalyst

Still, it remains to be seen how quickly Common Networks can provide its technology outside of the San Francisco Bay Area. Regulations in certain cities could delay expansion, and being first to market is an important advantage for Common Networks, Doherty said.

Verizon's 5G Home Internet is currently available in limited areas of Los Angeles, Sacramento, Houston and Indianapolis. Verizon is charging the same $50-per-month price for households with a Verizon wireless account and $70 per month for homes that don't. Verizon is also giving away the first three months of home Internet for free.

Still, the technology needs to work reliably in all neighborhoods, said telecommunications analyst Craig Moffett of MoffettNathanson. Architectures based on millimeter wave spectrum suffer from wave-strength issues, he said.

"The signal doesn't travel far enough, or penetrate through obstructions well enough, to make it economically interesting," Moffett said.

But Common Networks "may have a better story to tell than the larger incumbents" because they're "all about keeping costs down," said Moffett, citing the company's use of unlicensed spectrum and limited spending on traditional network facilities.

"It's not clear how successful companies like Common Networks will be competing against the large incumbent ISPs, but there's certainly a place for them in the broadband landscape," Moffett said. "I think you'll see more and more companies like Common Networks."

Brock and his fellow former Square co-workers Grace Chen, Mark Jen and Jessica Shalek were drawn to internet distribution because cable companies have scored poorly in customer service satisfaction surveys for decades.

"There's a clear problem to solve," Brock said.

Part of the company's pitch to consumers is to be the anti-cable company in terms of customer relationships, Doherty said. Stressing the importance of the first installation, Common Networks technicians, who are all full-time employees, are trained to make a positive first impression with customers to spread word-of-mouth buzz about the company. Still, the company's primary selling point will be underpricing its competition because of the company's proprietary technology, Brock said.

Convincing customers to choose a start-up over a widely known incumbent's service will be a challenge. Brock and his team are trying to market Common Networks' service locally, going door-to-door and working with city government officials to participate in local events to spread the word.

"It's very local, and it's effective," Doherty said. "There are a lot of levers to pull, getting positive press in local newspapers and using door hangers. It's old-fashioned, but people are looking for a cheaper experience."

Disclosure: Comcast owns NBCUniversal, the parent company of CNBC.

WATCH: Verizon CEO Hans Vestberg: Half of the US will have 5G phones by 2024

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Common Networks bets 5G wireless technology will replace cable internet in your home - CNBC

Why American Outdoor Brands, International Game Technology, and Dillard’s Jumped Today – Motley Fool

The stock market came under modest pressure on Thursday, although pullbacks were still relatively small and major benchmarks stayed close to their historic highs. Bad news on the earnings front from a major tech company weighed on investor sentiment, and some worried rising unemployment claims were a potential harbinger of economic weakness. Yet even with a slightly downbeat mood on Wall Street, some stocks managed to post strong gains. American Outdoor Brands (NASDAQ:AOBC), International Game Technology (NYSE:IGT), and Dillard's (NYSE:DDS) were among the top performers. Here's why they did so well.

Shares of American Outdoor Brands gained 6% after it announced that it would break itself up into two companies. American Outdoor said it would spin off its firearm business into a company to be known as Smith & Wesson Brands, with the remaining corporate entity keeping its original name and containing the outdoor products and accessories business. Board chair Barry Monheit said he expects the breakup to let each company "better align its strategic objectives with its capital allocation priorities." Given the challenges related to the gun business overall, it'll be interesting to see which stock performs better once the deal closes in the second half of 2020.

Image source: American Outdoor Brands.

International Game Technology saw its stock climb more than 23% after reporting its third-quarter financial results. Revenue for the slot machine and lottery specialistwas essentially flat from year-earlier levels, and adjusted earnings per share plunged 32% year over year. Yet investors seemed pleased with the numbers, in part because of how the company fought against higher taxes in the Italian market and particularly strong performance in the year-ago quarter. Moreover, IGT's outlook was reasonably solid, and those following the stock are excited about the innovative CrystalBetting terminal and how it could be an even bigger draw both for players and for the company.

Finally, shares of Dillard's rose 14%. The department store retailer surprised investors by making a modest profit in the third quarter of 2019, and even though both revenue and net income were down year over year, Dillard's managed to post flat comparable sales. That was an improvement from the previous quarter. CEO William Dillard said that "we were not satisfied with the third quarter," but shareholders seemed happy about the progress that Dillard's has made in fighting back against an extremely difficult environment for department-store retailers.

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‘Better evidence’ needed on immersive technology | News – ArtsProfessional

There is no clear evidence yet that immersive experiences can provide benefits to museums, according to a new report calling for more research into their potential.

The study from the Creative Industries Policy and Evidence Centre (PEC) and Cardiff University says cultural institutions have often been testbeds for immersive approaches to attracting audiences and deepening engagement, such as virtual reality, augmented reality and mixed media. But it argues that attempts to find straightforward connections between investment and outcomes have been inconclusive.

There is a seductive logic that the use of participatory and networked technologies leads to increased interaction between an experience and those [who] take part in it, it says.

The broad conflation between more interactivity or more control over a narrative and more agency should be challenged.

READ MORE:* New realities: exploring the potential of VR/AR*New fund to help develop immersive content* Museums and tourist boards at odds

The report advocates for testing the value of immersive technologies and other interventions in a way that adequately captures the nuances of immersion in museum, gallery and heritage contexts.

While immersive experiences can give greater voice to the stories of disenfranchised or underrepresented groups, the report warns that playing with the line between fact and fiction can be understood as provocative and political.

Debates about authenticity, re-creation and fakery are amplified by digital technologies and are particularly knotty in heritage contexts, it says.

The possibilities of re-creation come with increased responsibilities that designers and institutions need to take seriously, both for quality and ethical reasons.

Although it says immersive initiatives could challenge power relations in museum and heritage projects, the report notes possible tensions between these approaches and the current emphasis on economic returns within the research and policy landscape.

Co-Author Dr Jenny Kidd told ArtsProfessional that there is a need for more authoritative research and policy that underpins ethical concerns already being expressed in the sector. She says accessibility physical and economic environmental issues, privacy, data ownership, and the de-colonisation of museums all must be considered when introducing and implementing immersive technologies.

It seems a bit incongruous that in institutions where there has been so much effort in recent years to break down these barriers to access that we would reinstate them digitally.

There are further logistical challenges to the uptake and effectiveness of immersion experiences in the museum sector: cost, staff training, collaboration with partners, the usability of any immersive technologies, their sustainability beyond their initial use, and a lack of consensus on how to evaluate their impact can undermine these initiatives, the report says.

It recommends institutions consider how best to make [technology] frictionless, or even invisible. Although issues with internet connectivity and software glitches are likely to improve, such issues remain an unavoidable real-world aspect of many immersive encounters at this time.

The value of immersive experiences in museums can be complex to articulate given the levels of investment needed, it adds.

Kidd said their value will naturally be assessed if they are publicly funded, but remains optimistic about the potential of these interventions.

Theres positive work to be done in this space and I want to see us doing that work.

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'Better evidence' needed on immersive technology | News - ArtsProfessional

The emotional and financial cost of working with outdated technology – Ladders

It really is remarkable to think about how quickly computers made their way from revolutionary, clunky novelty, to sleek ubiquitous workplace companion. Of course, this innovation has come with a nearly equal share of benefits and setbacks some concrete, some indicative of larger issues.

What has been coined as the Technology Gap, or alternatively The Digital Divide or App gap if you like, locates the disparity between citizens that successfully interact with computers or mobile devices on a daily basis and those that do not. All the way back in 1942, political economist Joseph Schumpeter identified the broad implications of this imbalance as the most relevant feature of capitalism; Creative destruction.

With this in mind, our friends over at ZenBusiness conducted a survey to learn about the many complications that employees face when they are forced to work with outdated technology or a dearth of necessary office supplies, writting,

In 2019, the size of computers has shrunk, but their capabilities have exponentially grown. Despite advancements in this technology, though,accessibility continues to be a growing issueas the tech gap widens. As a result of this gap and other issues, many American offices are stuck with outdated technologies, low stocks of supplies, and a lack of integrated communication among teams.

More than 50% of the respondents say that their workplace technology was either moderately or completely outdated. According to the survey, computers caused workers the most difficulty, followed by printers, digital delays, cloud-based programs, cybersecurity, and general internet hiccups.

Employees reported that their workplace technology was moderately or completely outdated, a fairly low standard for workers. Specifically, computers (83.1%) and software (70.5%) were the most commonly outdated technology at work. As computers get older, they might not be able to support the newest iterations of a devices operating system, while outdated software inhibits an office from taking advantage of the latest developments in business technology, the authors explain.

As a consequence the average employee loses about 40 minutes of productivity a day, which further results in a $3,930 annual lost for employers. A routine lack of supplies additionally impacted productivity, leading to a median lost of $1,800 per week. Moreover,93% of workers say that trying to operate outdated technology severely affects their overalljob satisfaction. Thirty three percent of all of the employees surveyed say that they would even look for a new job due to their workplaces outdated technology.

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The emotional and financial cost of working with outdated technology - Ladders

Your Biggest Health and Fitness Questions, Answered! Join Our AMA With Katie Dunlop of Love Sweat… – POPSUGAR

Have a burning fitness question you'd like answered by a top health and fitness influencer? You're in luck: on Friday, November 22, at 1 p.m. ET/10 a.m. PT, certified personal trainer, fitness instructor, and entreprenuer Katie Dunlop of Love Sweat Fitness will be hosting a live Q&A session in our fitness Facebook Group, Healthy Living For Busy People With POPSUGAR Fitness. Our group is a space for our community to share their best tips for keeping personal health and wellness a top priority while facing the time constraints of a busy lifestyle.

As one of our featured fitness experts on our Instagram, Katie knows what it takes to build a healthy lifestyle whether it's in your dorm room or in between working, commuting, and living life. So join us! Our moderator and fitness editor Christina Stiehl will start a fresh chat thread within Healthy Living with Katie on standby to offer her advice and answer your questions.

The only catch? You'll have to join the Healthy Living For Busy People group for access. Click the "Visit Group" button ahead to get in the mix.

Image Source: POPSUGAR

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Your Biggest Health and Fitness Questions, Answered! Join Our AMA With Katie Dunlop of Love Sweat... - POPSUGAR

Would Kourtney Kardashian Be As Obsessed With Healthy Living If She Wasn’t Famous? – Showbiz Cheat Sheet

If youve been following Kourtney Kardashian for a while now, you know that she enjoys living a wholesome lifestyle.

Over the years, the reality star has been very vocal about healthy living and continues to maintain a clean diet as well as a regular workout schedule.

While being a prominent figure in the industry has a lot to do with Kardashians obsession with health and fitness, is it possible that shed still be into healthy living if she wasnt famous?

Even before Kourtney Kardashian become a world-famous reality star, her life fully revolved around wholesome living.

While Kardashian has taken her healthy lifestyle more seriously in recent years, the reality star comes from a family thats always embraced fitness and health.

During a 2011 interview with Shape, Kardashian revealed that she grew up living healthy all thanks to her family members.

My family has always been into exercise, she shared. My dad [the late Robert Kardashian Sr.-famous for defending O.J. Simpson] used to tape episodes ofSeinfeldandFriendsand watch them in the morning while he was on the treadmill.

When Kris Jenner married former Olympian Bruce Jenner, he encouraged the family to take Tae Bo classes as a way to exercise regularly.

Kim and I went almost every day after school, the reality star recalled. Sometimes wed do two classes in a row because we had so much energy.

As Kardashian got older, she continued putting in work when it came to her work out regimes.

She even discovered her love for running, which she continued doing right up until she was seven months pregnant with her first child. But carrying an extra 40 pounds started to bother my knees, she said, so I had to stop.

Although exercising and healthy eating are things Kardashian has always loved doing, she didnt embrace a completely healthy lifestyle until her oldest son, Mason, was born.

Before she even gave birth, the reality star was adamant on raising her family on a strictly organic diet and putting for that lifestyle for her child, Kardashian soon found herself adopting the same eating habits.

My mom gave me the Beaba baby food maker that steams and purees fruits and veggies, she shared.

I use only organic foods for him, and it made me think about what I put into my body too, she continued. I couldnt sit around eating cookies and expect him to eat vegetables. I fell in love with salmon, which I hardly ever ate before. And I used to eat salads, but now Im having side dishes like spinach and carrots too. It wasnt just because its good for me-Ive discovered I really liked eating that way.

Ever since then, Kardashian has enjoying living a healthy lifestyle and even shares some of her health tips and recipes with fans on her website, Poosh.

Back an April, the reality star launch the site and it quickly became the central hub for all things health and wellness, life and style, interior design, beauty, motherhood, and more.

Although she frequently talks about healthy living on social media as well as Keeping Up with the Kardashians, Kardashian wanted to create a space where people could really educate themselves on the topic.

I decided to launch Poosh because I felt that there was something missing in the healthy lifestyle space, she wrote on her site. Healthy living gets a bad rap; its as though if you careaboutwhat you put in or on your body, then youre not sexy or cool. But this just isnt true, and Poosh is here to prove just that.

Though Kardashian has made a very lucrative career out of her healthy lifestyle, many of us cant help but wonder if shed still be this invested in health and wellness if she wasnt a famous reality star?

While being a world famous celebrity has given Kardashian the tools shes needed to continue living healthy, were pretty sure she still would have adopted the same lifestyle even if she wasnt in the public eye.

Before she was even a well-known reality star, Kardashian was just a Calabasas native who loved all things health and fitness.

She would constantly work out and enjoyed eating things that were good for her body.

If Kardashian never became a reality star, were certain she would have still adopted her healthy lifestyle and continued to put in the work to maintain it.

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Would Kourtney Kardashian Be As Obsessed With Healthy Living If She Wasn't Famous? - Showbiz Cheat Sheet

Toms River Pediatrician, Dr. Charita Y. Csiky Featured in 2019 Top Doctor Edition of Healthy Living Magazine – Benzinga

SCOTCH PLAINS, N.J., Nov. 14, 2019 /PRNewswire-PRWeb/ --Dr. Charita Y. Csiky of Pediatric Affiliates in Toms River, New Jersey was reviewed and approved by NJ Top Docs for 2019. Dr. Csiky is a board certified pediatrician and was recently featured in NJ Top Docs' 2019 Top Doctor edition of Healthy Living.

Found on page 46 in Healthy Living's Highlighted Directory, Dr. Csiky is featured among other reviewed and approved pediatricians across New Jersey.

The goal of NJ Top Docs' magazine, Healthy Living is to provide New Jersey residents with a complete informational resource to assist them in choosing a local highly qualified healthcare provider at no cost.

Dr. Csiky has been featured in each Top Doctor edition of Healthy Living since 2015. 2019 marks Dr. Csiky's fifth consecutive year of being reviewed and approved as a NJ Top Doc.

To learn more about Dr. Csiky and her practice, please visit: https://njtopdocs.com/nj-doctors/charitacsikymd/.

About Us NJ Top Docs a comprehensive, trusted and exclusive healthcare resource featuring reviewed and approved Top Doctors and Dentists in New Jersey online in an easy to use format. NJ Top Docs only reviews and approves providers based on merit after they have been extensively vetted.

NJ Top Docs is a division of USA Top Docs which allows patients to meet providers online before making their appointment.

For more information, please click here to contact us or visit http://www.NJTopDocs.com.

You can also follow us on Facebook and Twitter.

SOURCE NJ Top Docs

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Toms River Pediatrician, Dr. Charita Y. Csiky Featured in 2019 Top Doctor Edition of Healthy Living Magazine - Benzinga

Southern Cancer Center Thanksgiving Food Drive | Healthy Living – FOX10 News

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Family finds fitness and much more at YMCA – GazetteNET

Published: 11/13/2019 8:17:10 AM

Healthy living is a core value in our family. When we relocated, from Boulder, Colorado, we considered a number of communities in New England before deciding to move to Northampton. We were attracted by the extensive network of bike paths, abundance of local food production, access to forests and trails, a culture of higher learning and a multitude of resources for raising a family.

Still, during our early years in Northampton, Lori and I struggled with the reality that raising two young children, Zoe and Adam, in the absence of local friendships or family, was challenging. In particular, finding ways to remain physically active something Lori and I had come to take for granted while living in the outdoor recreational mecca of Boulder was essential to our well-being and sanity.

Fortunately, Northampton is home to the Hampshire Regional YMCA. Once we learned that Y membership included access to free child care through the Child Watch program and its experienced, well-trained providers we quickly decided the cost of membership was a worthy addition to our frugal family budget.

It was not long before Lori and I came to appreciate that the Y focused more broadly on healthy living, including spirit, mind and body, and was not just a place to exercise. So, over the years, in addition to regular cardio workouts in the gym and laps in the pool, we have enjoyed yoga and meditation classes, family mindfulness retreats at the Ys summer camp facility, and blissful sits in the steam room during long New England winters. As a bonus, since Y memberships include reciprocal benefits with many YMCAs in the U.S., Lori and I have exercised for no additional cost at other Ys in Massachusetts as well as in Connecticut, New Hampshire, Florida and Illinois, when traveling for work or visiting family.

As Zoe and Adam, teen and tween respectively, have aged, they have increasingly benefited from the Ys focus on youth develop, including swim lessons, open gym, open swim, the youth lounge and gymnastics program. These activities have offered a positive, social setting to develop their growing bodies, build friendships, and be part of a larger community committed to wellness.

One of the greatest and unexpected benefits of the Y, for Lori and me, is the way the center acts as a hub for social connection. Every visit to the Y offers an opportunity for brief yet enriching connection with friends, kids of all ages, other parents, professional colleagues, and acquaintances, reminding us that we are part of a larger community committed to healthy living. And for many years, Lori has taught Pilates classes at the Y, offering her both a circle of colleagues and connection to many throughout the community.

Recently our family participated in the Ys 8th Annual 5k Run. The weather was glorious, mid-40s and sunshine, with colorful, fallen leaves underfoot as we paced our way along the bike path and looped around picturesque Look Park. Lori and Zoe helped set up for the event. Zoe, along with friends from the Y gymnastics team stationed themselves on the course to cheer runners and walkers. Lori, who has recently returned to running, logged her best 5k time in decades. Adam and I ran together, and he out sprinted me at the end to log a personal best time.

After the course cleared, we gathered inside to hear race results. More importantly, the race director shared words about the Y community and the importance of the event. I was astonished to learn that in the prior year, the Y had devoted over $270,000 toward scholarships and discounted memberships, in order to help make the Y accessible to even more children and families.

I left the event with even greater appreciation for all the Y has to offer. Initially we joined the Y as a place to workout, but weve chosen to stay for the many ways it helps us sustain a healthy lifestyle, including connection to the community we now call home.

John Engel of Florence can be reached through his website fatherhoodjourney.com.

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Family finds fitness and much more at YMCA - GazetteNET

Moderate Wine Consumption Linked to Lower Risk of Lung Disease – Wine Spectator

When it comes to respiratory illnesses, there are few studies exploring the link between alcohol consumption and lung health. However, new research from Sweden appears to breathe life into this field: Its findings suggest that moderate alcohol consumption may lower the risk of lung disease in men.

Chronic Obstructive Pulmonary Disease (COPD) is a respiratory illness that restricts airflow into and out of the lungs, making breathing difficult. The illness advances over time, increasingly diminishing pulmonary performance, often with fatal results.

The leading cause of COPD is smoking, followed by asthma and environmental factors. Symptoms of COPD include a cough that produces a lot of mucus, shortness of breath, especially during physical activity, wheezing and chest tightness, according to the U.S. National Library of Medicine. The disease impacts an estimated 16 million people yearly in the U.S. alone. "According to investigators in the Global Burden of Disease Study, COPD was the third leading cause of loss of life in the United States and the fourth leading cause in the United Kingdom in 2016," the study authors write.

The study, conducted by a team from Sweden's Karolinska Institute and the U.K.'s University of the West of England, Bristol, and published in the American Journal of Epidemiology, involved over 44,000 men between the ages of 45 to 79. Researchers began tracking the men, starting in 1998, to the moment they were diagnosed with COPD or until the end of 2014. The study took into account the subjects' health, age, weight, body mass index, level of education, economic class and various other factors.

The median age of the participants was 60. Of those, 24.4 percent were smokers, 38.5 percent were ex-smokers, and 35.8 percent had never smoked. Participants were also asked how much they drank per week. The researchers defined 1 standard drink as 12 grams of ethanol, approximately 5 ounces of wine. (That's slightly lower than the 14 grams defined as a standard glass of wine by the U.S. Office of Disease Prevention and Health Promotion.)

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The study found that moderate drinkers had a lower incidence of COPD than both abstainers and heavy drinkers. In fact, the individuals who didn't consume alcohol had a 21 percent higher incidence of the disease than individuals who drank moderately, roughly 7 to 14 drinks per week. Heavy drinkers (those consuming more than 20 drinks per week) had a 34 percent higher incidence of COPD than moderate drinkers.

The researchers were careful to make sure they adjusted their results to take into account possible confounding factors. The data revealed that wine drinkers are more likely to have higher incomes as opposed to liquor drinkers, and liquor drinkers are also more likely to be smokers. Also, those who consumed one or more glasses of wine per week tended to have a college education. Income and smoking are both factors that affect health outcomes and the incidence of COPD. However, even after adjusting for these confounding factors, the researchers still found that moderate drinkers had lower risk factors for COPD than non-drinkers and heavy drinkers.

"We can hypothesize that the protective association for moderate alcohol consumption, especially beer and wine consumption, relates to the antioxidant impact of polyphenols present in alcoholic beverages," the authors write. However, because the researchers had little information on other COPD causes (like chemical fumes, pollution, etc.), aside from smoking, they were not able to conclusively say that moderate drinking alone was the only factor in these positive outcomes. Further research on the antioxidant qualities of wine and beer may help bolster these findings.

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Moderate Wine Consumption Linked to Lower Risk of Lung Disease - Wine Spectator

I Want to Be Healthier: Q&A with Traverse City Behavioral Health Specialist Mandy Remai – MyNorth.com

Are you ready to make a healthy lifestyle change but dont know where to start? Advice can come from all directions, and it can easily become overwhelming. Heres a simple solution: Start small. We consult with Traverse City Behavioral Health Specialist Mandy Remai, who works at Munson Healthcares Healthy Weight Center and also owns a private practice, Seasons of Life Counseling, about how to make healthy changes to your environment and your mindset.

As a counselor who specializes in weight and eating issues, can you tell us a bit about your background?

Munsons Healthy Weight Center is a comprehensive program which includes nutrition, exercise, behavioral health and medical components. As the Behavioral Health Specialist for the program, I help clients incorporate long-term behavioral changes by developing strategies and tools that fit with their individual situations.

I have had my practice in Traverse City for 15 years. Helping people with weight and eating issues continues to be my speciality, but my practice has grown. My strength as a counselor is helping people feel better by showing up for themselves the same way they show up for everyone else. So many of us are good at taking care of other peoples needs, meeting our responsibilities, but we end up neglecting our own needs. This leaves us feeling empty and drained. When we start prioritizing our own health and well-being in all areas of our lives, everything improves. We do better when we feel better and everyone benefits from this!

How do you implement healthy living in your daily life?

Exercise and nutrition are important to me. So is journaling, reading and having quiet time each day to check in with myself. When my eating or mood is off, its usually an indication that something in my life needs attention. Maybe its a difficult conversation with someone, more sleep or just some time to relax and recharge. We pay attention to whats going on with others we care about, and being healthy means we also pay attention to whats going on with ourselves.

Read Next: 6 Great Healthy Lifestyle Apps for Food, Exercise and Sleep

For those striving to be healthy, would you recommend changing their environment?

Changing our environments can make a big difference. But I strongly believe that the starting point is changing our thinking. If we want to generate different external results, we need an internal shift. Changing the way we think about food, exercise, self-care. Taking good care of ourselves so we can be healthy and feel good versus using will power and discipline to lose weight.

What tips do you have for people on changing their environment for success?

I would start with deciding why you really want to make some changes. People will say they want to get healthy. What does that mean specifically to you? Do you want to have more energy for your life? Do you want to get off some medications? Do you want to be able to complete a 5k? Do you want to feel more comfortable in your body? Do you want to enjoy your life more? What do you want?

And the next question is: What are you willing to do to get it? What changes are you willing to make? Not what should you do, what are you willing to do. Pick one behavior you know you are willing to do and start with that. Be reasonable with your expectations and set yourself up for success.

How about when it comes to screen time?

Screen time can be a problem for a lot of us. I think its important that we start thinking about how we really want to be spending our time. What do we enjoy doing? What gives us energy and helps us feel excited? What do we have fun with? Are we really enjoying the screen time or are we just using it to zone out because we are feeling tired and worn out?

Making conscious choices about what really matters and what we can let go of. And again, being reasonable. What can work for you?

Read Next: Healthy, Fun Ways to Reduce Screen Time for Kids and Adults

Do you have suggestions for families?

I think each family can be a little different depending on their personal preferences. Get input from the kids about what sounds fun to them. Bundling up and going for a walk or going sledding might not sound like fun but once you get out there and do itit usually is! Getting started is often the hardest part.

To take baby steps toward implementing these changes, what would be the first step youd recommend people try?

Again, what are you willing to do? Drink more water? Take a walk most days of the week? Take time to meal plan and prep for the week? Have a family outdoor adventure each week? Have limited screen time each day? Pick one.

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I Want to Be Healthier: Q&A with Traverse City Behavioral Health Specialist Mandy Remai - MyNorth.com

No, e-bikes arent cheating – The Verge

A common complaint you hear about electric bikes is that they basically amount to cheating. Cycling, especially mountain biking, is supposed to be about exercise and promoting healthy living, but how healthy can you be when the bikes motor and battery are doing most of the work for you? A new study out last month the first to investigate the health effects of pedal-assist electric bikes puts to rest many of these misconceptions.

Researchers from Brigham Young University recruited 33 subjects, mostly men between the ages of 18 and 65, to ride both regular mountain bikes and electric, pedal-assist mountain bikes on a rolling six-mile, single-track course through the Utah countryside. Afterward, they compared their heart rates and found that riding an e-bike is no effortless fling. In fact, it requires almost as much physical exertion as riding a traditional mountain bike.

Riding both types of bikes placed the vast majority of participants in the vigorous-intensity heart rate zone, the study authors concluded. The average heart rate of a test subject riding an e-bike was 93.6 percent of those riding conventional bikes. Moreover, electric bikes appear to be an excellent form of aerobic or cardiovascular exercise, even for experienced mountain bikers who regularly engage in this fitness activity.

The researchers also surveyed their test subjects, both before and after riding, to determine their attitudes toward e-bikes. Some said their preconceived notions were confirmed, while others admitted the experiment subverted their beliefs. Most were positive toward e-bikes before the test, with only 18 percent saying they were opposed. Some attitudes changed, though, with fewer participants willing to admit after the test that e-bikes were just a passing fad.

Most importantly, the vast majority of the test subjects said they didnt feel like they got a workout while riding an e-bike despite heart rate monitors and fitness trackers indicating that most participants experienced vigorous levels of exercise. This raises the possibility that e-bikes could be well suited in helping both experienced cyclists and more sedentary individuals to meet their physical fitness goals.

Exercise that doesnt really feel like exercise seems like a pretty major breakthrough, especially if the goal is to get more sedentary individuals off the couch and into a more active lifestyle.

One area of concern identified by the BYU team was speed. Rider speeds on the e-bikes were four miles per hour faster on average. But the higher speeds achieved on an e-bike could impact peoples negative perceptions of them. For example, an e-bike rider who rudely passes other cyclists on a bike path could ultimately harden some opinions toward e-bikes. More research will be needed before making any determinations, though.

There have been a handful of smaller studies on the health effects of e-bikes, but the BYU study, published in the Journal of Medical Internet Research, is notable for its larger sample size. A small study in Boulder, Colorado, in 2016 found that a month of commuting on an e-bike improved fitness and blood sugar levels. Most participants also said they spent more time in the saddle than the study authors required, mostly because they were having so much fun.

Overall, participants in the BYU study were more accepting of e-bikes after riding one. The adage dont knock it until you try it, the study authors conclude, appears applicable with pedal-assist technology.

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No, e-bikes arent cheating - The Verge

How to eat and live healthy in a Swiggy-Zomato-Uber Eats world – BusinessLine

A few years ago, mom told us that everyone at work was talking about it. So, I also gave the GM diet a try. Soon, it made me weak and I was not enjoying it. Over the years, juggling between not eating carbs or eating only fruits and soup, I fainted a few times due to low sugar or low blood pressure, says Aishwarya Bhosale (name changed), a 24-year-old post-graduate student. The GM diet, also known as the General Motors diet, promises rapid results for those looking to lose body fat rapidly, by having them eat select foods. It was apparently devised by the carmaker to help its employees lose weight.

Heena Parmar, a Chennai-based professional in the event management industry, has tried numerous diets because of her sedentary lifestyle. I saw a social media influencer posting her food habits, swapping rice with cauliflower rice, whole-wheat chapatis with coconut-flour rotis... A lot of other influencers were doing the same. Without doing much research I just followed her. A few days into the diet, I was ill and unhappy. The story is pretty much the same with Riya, Shruti and Rohan, all of whom have been wrestling with food and lifestyle changes.

While junk food has proliferated and is just a keypad touch away from ones doorstep, the same is the case with healthy food. The only problem: what exactly should one eat? Awareness about the need to eat and live healthy has grown but there are millions of advisers out there and it is hard to know which one to follow. A simple Google search can get you in touch with the diets of skinny models and actors. Hashtags such as #cleaneating, #detox and #diet will lead you millions of pictures on Instagram. Millions world-over watch lifestyle and diet videos on YouTube the latest fad is watching various What I eat in a day videos. The ample number of diets veto (vegan keto), paleo, Atkins, vegan, raw food, only-fruit, gluten-free and so on has created a sumptuous cocktail of myth and confusion.

According to a study by management consultancy RedSeer, entitled Indian habit of being healthy, India is home to 90 million Health Conscious Individuals (HCI). And, it says, this figure will touch 130 million by 2020.

The RedSeer report also states that a large segment of the HCIs consist of people who are highly concerned and aware of fitness needs but only make partial efforts.

Vicky Sinha, running on the corporate hamster wheel, says eating healthy food is a task. With everything being delivered at the doorstep, I keep slipping in and out of my diet plans. I think I might now start an intermittent fasting diet; I saw it on Instagram a few days ago, he says.

These short-term, quick-result diets give instant gratification. But they can also have adverse effects, both mentally and physically. Riya Chauhan, a college student, says she tried the only soup for dinner diet method. It gave me instant results. But once I stopped it, I gained double the weight I lost, in no time.

Vicky says that his mundane, desk job gives him no time to exercise. That is why he keeps trying the fancy diets. These diets help me feel a little less guilty of being in an unhealthy life situation, he says.

Kannan Raman, Nutritionist and co-founder of Daily9, a lifestyle coaching digital entity, says the most important aspect of living healthy is to sleep well at least for eight hours; eat well focussing on quality rather than quantity; and exercise regularly, in that order.

And if one wants to follow a diet, he says, simple and boring wins every time. Start slow and do not stop. Kannan has a basic thumbrule for anyone who wants to go on a diet. Do you see yourself sustaining this for a year? If the answer is no, then maybe this is not for you. Anyone who wants to go on a diet, he says, should be mentally prepared for change and be ready to sustain that change over the long term.

Dr M Meenakshi Sundaram, a Chennai-based general physician, says jumping on to the crash-diet bandwagon may not necessarily be the best choice for a person. Dietary restrictions are not required for youngsters, he says. People who are young and active need foods that will fuel their energy, and that has to come from carbs, he says.

Another myth that is being followed is the no-whites diet, which he says is not good for health, especially for youngsters. Salt, sugar and rice are required by the body. The brain needs sugar to function and the body needs some amount of salt. Youngsters should not avoid these completely.

Kannan and Dr Meenakshi both recommend that people stay close to their roots and eat the food from their culture rather than venturing out, in a manner of speaking, to distant places such as the Mediterranean to follow fancy diets alien to them. Any change in the diet should happen gradually, Kannan says. For instance, by adding an extra vegetable in meals, or gradually increasing the portion size of vegetables.

In the end, says Aishwarya Bhosale, who tried the GM diet: It is best to consult an expert and get a tailor-made diet, to avoid repercussions.

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How to eat and live healthy in a Swiggy-Zomato-Uber Eats world - BusinessLine

Erythrocyte leveraged chemotherapy (ELeCt): Nanoparticle assembly on erythrocyte surface to combat lung metastasis – Science Advances

Abstract

Despite being the mainstay of cancer treatment, chemotherapy has shown limited efficacy for the treatment of lung metastasis due to ineffective targeting and poor tumor accumulation. Here, we report a highly effective erythrocyte leveraged chemotherapy (ELeCt) platform, consisting of biodegradable drug nanoparticles assembled onto the surface of erythrocytes, to enable chemotherapy for lung metastasis treatment. The ELeCt platform significantly extended the circulation time of the drug nanoparticles and delivered 10-fold higher drug content to the lung compared with the free nanoparticles. In both the early- and late-stage melanoma lung metastasis models, the ELeCt platform enabled substantial inhibition of tumor growth that resulted in significant improvement of survival. Further, the ELeCt platform can be used to deliver numerous approved chemotherapeutic drugs. Together, the findings suggest that the ELeCt platform offers a versatile strategy to enable chemotherapy for effective lung metastasis treatment.

Cancer has been one of the leading causes of mortality over the last few decades (1). While early detection of tumor cells in specific tissues or the blood has improved the survival of patients with cancer, current standard-of-care interventions, including surgery, radiation therapy, or chemotherapy, have limited efficacy if cancer is not detected early (14). Early detection, however, is not often feasible, and in most patients, tumors have metastasized to secondary locations by the time of diagnosis (2, 4).

According to the National Cancer Institute, the most common site of metastasis for a variety of primary cancers is the lung, owing to its high vascular density. Lung metastasis is highly fatal if not treated, and currently, there is no specific treatment for it (5, 6). Systemic chemotherapy is one of the standard treatment options for lung metastasis (7, 8). However, its efficacy has been far from desirable due to ineffective targeting and poor accumulation in the lungs. Nanotechnology has played a pivotal role in enhancing the treatment of advanced metastatic cancers (911) and therefore can be applied in the case of lung metastasis as well. However, traditional nanoparticle (NP) delivery often fails to accumulate at the desired site of action due to the existence of biological barriers that impede the intravascularly injected NPs (1217). Active targeting using tissue-specific ligands has often been explored as a strategy to improve tissue accumulation but has only resulted in modest improvement of therapeutic efficacy and decreased translational capability due to increased cost of production (1826).

To achieve efficient drug delivery to enable chemotherapy for effective lung metastasis treatment, we used the unique physiology of the target site and developed a two-pronged strategy [erythrocyte leveraged chemotherapy (ELeCt)]biodegradable drug NPs assembled on the surface of erythrocyte (Fig. 1A). Erythrocytes act as a primary drug delivery system, capable of responsively dislodging the particles in the lung endothelium and tumor nodules in response to the high shear stress experienced by erythrocytes in narrow lung capillaries (27, 28). The biodegradable NPs themselves are capable of encapsulating large amounts of chemotherapeutics and having a characteristic controlled-release mechanism (29, 30). They act as a secondary drug delivery system enabling sustained delivery of the cargo. In this study, superior accumulation and therapeutic efficacy of this lung physiology-assisted NP strategy were demonstrated using a model chemotherapeutic doxorubicin (DOX). This concept was successfully used to combat lung metastasis and improve survival in early- and late-stage melanoma lung metastasis models. The ability to incorporate a plethora of current clinical chemotherapy drugs and drug combinations in the biodegradable NPs and subsequently assemble onto the erythrocytes was demonstrated. The particles also readily assembled to human erythrocytes and dislodged in a shear-dependent manner. Together, ELeCt offers a versatile, potent, and translatable platform to combat lung metastasis.

(A) Schematic illustration of the composition and mechanism of the biodegradable drug NP assembling on the erythrocyte platform (ELeCt) for lung metastasis treatment. (B to D) Average size (B), zeta potential (C), and drug loading contents (D) of plain and drug-loaded NPs. (E) SEM images showing the morphological features of the NPs. Scale bars, 200 nm. (F) Size distribution of plain and drug-loaded NPs. (G) Drug release kinetics from the biodegradable NPs in a complete medium (n = 4). (H and I) Flow cytometry histogram plots (H) and CLSM images (I) showing the interaction of drug-loaded NPs with B16F10-Luc melanoma cells. In (I), cell nuclei were stained using 4,6-diamidino-2-phenylindole (DAPI). (J and K) Dose-response curve (J) and median inhibitory concentration (IC50) values (K) of B16F10-Luc cells after being treated with different formulations for 24 hours (n = 6). n.s., not significantly different (Students t test).

We used DOX as a model drug and prepared drug-loaded biodegradable polymeric [poly(lactic-co-glycolic acid) (PLGA)] NPs using the nanoprecipitation method. The drug-loaded PLGA NPs had a diameter of 136.0 2.7 nm, which was slightly larger than the plain NPs (Fig. 1B). The encapsulation of DOX made the surface of the drug-loaded NPs slightly positive (10.45 0.84 mV) (Fig. 1C), and this can be attributed to the presence of DOX on the NP surface. The drug-loaded PLGA NPs exhibited a high drug loading capacity (196.7 5.8 mg/g) (Fig. 1D). We characterized the morphology of the NPs using scanning electron microscopy (SEM). SEM images shown in Fig. 1E revealed that both the plain and the drug-loaded PLGA NPs were spherical and relatively monodispersed. The dynamic light scattering data (Fig. 1F) confirmed the uniform size distribution of the prepared NPs. To test whether the drug could be released from the PLGA NPs, we assayed their release profile in a complete medium. A burst followed by a sustained-release profile was observed, and most of the drug was released within the first 6 hours (Fig. 1G). Efficient interaction of drug NPs with the target cancer cells is critical for successful drug delivery and efficacy. In this study, we used B16F10-Luc melanoma cells as a model to evaluate the interaction between the drug-loaded biodegradable PLGA NPs and the target cancer cells. As shown in Fig. 1H, the drug-loaded PLGA NPs appeared to be internalized by B16F10-Luc cells quickly and efficiently. Within 20 min of the incubation, a substantial portion of the cells had drug-loaded NPs in them. The confocal laser scanning microscopy (CLSM) images shown in Fig. 1I confirmed the efficient interactions between the NPs and the B16F10-Luc cells. Noticeably, the increase in DOX fluorescence within the cell nucleus suggested an effective intracellular delivery and sufficient release of the loaded drug. We further evaluated the in vitro antitumor efficacy of the drug-loaded PLGA NPs in a two-dimensional culture of the same cell line. As indicated by the dose-response curve (Fig. 1J) and IC50 (median inhibitory concentration) values (Fig. 1K), the drug-loaded PLGA NPs exhibited a slightly weaker cell killing efficacy compared with the free drug. However, the difference between them was not significant.

We first evaluated whether the drug-loaded PLGA NPs could efficiently assemble onto the mouse erythrocytes. To do this, we incubated mouse erythrocytes with the NPs at a range of NP-to-erythrocyte ratios (50:1 to 800:1) and detected the binding of NPs using flow cytometry. As shown in Fig. 2 (A and B), the drug-loaded PLGA NPs indeed assembled onto the mouse erythrocytes efficiently. Particularly, 81.6% of erythrocytes were found to carry NPs when being incubated with NPs at a ratio of 200:1, and this number increased to >96% on further increasing the incubation ratio. The binding efficiency of the NPs to the erythrocytes was also quantified. Unexpectedly, a substantial portion (39.3 to 54.5%) of the incubated NPs assembled onto the mouse erythrocytes, depending on the feed ratio of the NPs to the erythrocytes (Fig. 2C). Because of this high binding efficiency and the high drug loading capacity of the NPs, the mouse erythrocytes were able to carry a high drug dose (as high as 294.1 g per 3 108 erythrocytes) (Fig. 2D). In addition, the drug dose on the mouse erythrocytes could be easily tuned by manipulating the feed ratio of the NPs to the erythrocytes. Next, we visualized the assembly of drug-loaded PLGA NPs onto the mouse erythrocytes using CLSM and SEM. As shown in Fig. 2 (E and F), both the CLSM and SEM data confirmed the efficient assembly of the NPs onto the mouse erythrocytes. Meanwhile, the mouse erythrocytes maintained their biconcave shapes after being hitchhiked by the drug-loaded PLGA NPs (Fig. 2 (E and F)), indicating the assembly of the NPs had caused minimal damage to the carrier erythrocytes. To test the translational potential of the erythrocyte hitchhiking platform, we evaluated the assembly of the drug-loaded PLGA NPs onto the human erythrocytes. Both the CLSM and SEM images shown in Fig. 2 (G and H) suggested that the drug NPs could efficiently assemble onto the human erythrocytes as well. In addition, we also evaluated the assembly of drug-loaded PLGA NPs to human erythrocytes at different NP-to-erythrocyte feed ratios (200:1 to 1600:1). Similar to the murine counterparts, the drug-loaded PLGA NPs assembled onto the human erythrocytes with high efficiency (38.7 to 45.7%) at various NP-to-erythrocyte feed ratios (Fig. 2 (I and J)). Moreover, the drug dose on human erythrocytes could be tuned by changing the incubation ratio, and a very high drug dose (209.1 g per 1.5 108 erythrocytes) could be hitchhiked to human erythrocytes when being incubated at a 1600:1 NP-to-erythrocyte ratio (Fig. 2K).

(A) Flow cytometry analysis of assembly of DOX-loaded PLGA NPs to mouse erythrocytes at different NP-to-erythrocyte ratios (left to right: 0:1, 50:1, 200:1, 400:1, and 800:1). (B) Percentage of mouse erythrocytes carrying at least one NP. (C) Nanoparticle binding efficiency and (D) drug dose on mouse erythrocytes at different NPtomouse erythrocyte ratios. (E) CLSM and (F) SEM images of mouse erythrocytes with drug-loaded NPs assembled on them. Scale bars in (F), 2 m. (G) CLSM and (H) SEM images of human erythrocytes with drug-loaded NPs assembled on them. Scale bars in (H), 2 m. (I) Flow cytometry assay of the assembly of drug-loaded NPs to human erythrocytes at different NP-to-erythrocyte ratios (left to right: 0:1, 200:1, 800:1, and 1600:1). (J) Nanoparticle binding efficiency and (K) drug dose on human erythrocytes at different NP-to-erythrocyte ratios.

We first conducted a pharmacokinetic study to examine the blood circulation time of different drug formulations. As shown in Fig. 3A, by assembling drug NPs to erythrocytes, a higher drug concentration in the blood was achieved at all the tested time points, indicating an extended circulation time of the hitchhiked formulation. Mouse lung capillaries have an average diameter of 5 m, narrowing down up to sizes as small as 1 m, three to four times smaller than the mouse erythrocyte diameter (27). Upon intravenous administration, the drug-loaded NPs assembled onto erythrocytes are expected to detach from the carrier erythrocytes because of the high shear stress and be deposited in the narrow lung capillaries. To test this hypothesis, we first performed an in vitro shear study in which the erythrocytes carrying the drug-loaded NPs were sheared for 20 min at a low (~1 Pa) or high (6 Pa) shear stress. As shown in Fig. 3B, detachment of the drug NPs from the mouse erythrocytes was evidently shear dependent, providing a basis for specific delivery of drug NPs to the diseased lungs. Particularly, 76% of the hitchhiked drug NPs were sheared off at the lung-corresponding shear stress (6 Pa), using a rheometer. Moreover, this shear-dependent detachment of drug NPs was also observed with the human erythrocytes, bolstering the translational potential of this ELeCt platform. To test whether the drug NPs could be sheared off and deposited in the lungs that bear metastasis in vivo, we conducted a biodistribution study in mice bearing B16F10-Luc melanoma lung metastasis and quantified the amount of drug, in this case DOX. As shown in Fig. 3 (C and D), by assembly onto erythrocytes, the drug-loaded NPs delivered 16.6-fold higher drug content to the diseased lungs as compared with their free NP counterparts, 20 min after administration. Even at a longer time point (6 hours), erythrocyte hitchhiking deposited 8.7-fold higher drug content in the lungs as compared with their unhitchhiked counterparts. In addition, erythrocyte hitchhiking delivered a 6.9-fold higher drug content to the lungs with melanoma metastasis as compared with the free drug injection, 20 min after administration. Next, we investigated the distribution of the drug NPs sheared off from the carrier erythrocytes within the lungs bearing metastasis. As shown in Fig. 3E, consistent with the biodistribution data, more drug NPs were found in the lung section being treated with erythrocytes with NPs assembled on them compared with that being treated with the NPs alone. Evidently, a substantial portion, although not all, of the deposited NPs went deep into the tumor metastasis nodules, suggesting the biodegradable drug NP assembling on erythrocyte was able to precisely deliver the payload chemotherapeutic agents to their desired site of action.

(A) Pharmacokinetics of intravenously administered DOX formulations. Extended blood circulation time of DOX was achieved by erythrocyte hitchhiking compared with using free drug or NPs alone (n = 3). Significantly different [one-way analysis of variance (ANOVA)]: *P < 0.05 and **P < 0.01. (B) Hitchhiked drug-loaded NPs could specifically detach from mouse and human erythrocytes under the lung-corresponding shear stress. Samples were sheared for 20 min (n = 3). Low shear indicates rotary shear (~1 Pa), while high shear was at 6 Pa. Significantly different (Students t test): ***P < 0.001. (C) Drug accumulation in the lungs of mice bearing B16F10-Luc lung metastasis at 20 min and 6 hours after intravenous administration of different DOX formulations (n = 3). Significantly different (one-way ANOVA): *P < 0.05 and ***P < 0.001. (D) Comparison of the drug concentration in the lungs of erythrocyte hitchhiking group to that of the free drug and NP-alone groups (n = 3). (E) Drug distribution in the diseased lungs 20 min after intravenous administration of DOX formulations. Dashed lines indicate the edge of metastasis nodules.

To evaluate the efficacy of the biodegradable drug NP assembly on the erythrocyte platform, we established a B16F10-Luc melanoma lung metastasis model and tested the antimetastatic efficacies in both the early and the late stages of the same model. We first tested the efficacy of the developed platform in controlling early-stage lung metastasis. As shown in Fig. 4A, the lung metastasis model was established by intravenously injecting B16F10-Luc cells via the tail vein. Four doses of treatments were given every other day with the first dose being administered 1 day after the tumor cell injection. The lung metastasis burden was measured by the bioluminescence intensity in the lung. As indicated by the bioluminescence images (Fig. 4B) and lung metastasis burden growth curve of individual mouse (Fig. 4C), a significantly better inhibition of the lung metastasis progression was achieved by the ELeCt as compared with using the free drug or NPs alone. Two mice remained completely free of lung metastasis after being treated with the drug NPs assembled on erythrocytes for up to day 31 after tumor inoculation. We also calculated the overall lung metastasis burden based on the bioluminescence intensity in the lungs. As shown in Fig. 4D, in the first 23 days after tumor inoculation, lung metastasis was almost completely inhibited in all mice being treated with the drug NPs assembled on erythrocytes. Particularly, as shown in Fig. 4E, on day 16, free drug and drug NPs alone resulted in a 17.2- and 1.8-fold lower average bioluminescence intensity compared with the control, respectively. In a sharp contrast, ELeCt achieved a 204.8-fold lower average bioluminescence intensity compared with the control. Similar finding was also observed on day 23. As shown in Fig. 4F, compared with using the drug NPs alone, the treatment using drug NPs assembled on erythrocytes led to a 302-fold lower average bioluminescence intensity. The Kaplan-Meier survival analysis (Fig. 4H) further confirmed the significantly improved survival benefit of the ELeCt approach over using the NPs alone. The use of the free drug or NPs alone only improved survival slightly, increasing the median survival time from 29 to 32 days. In a sharp comparison, by the treatment with drug NPs assembled on erythrocytes, the animal median survival time was extended from 29 to 61 days. Moreover, one of seven mice continued to survive for at least 70 days. We also monitored the body weight change of mice during the entire treatment period. No significant body weight loss was detected for any of the treatments, compared with a sharp decline in the body weight during the free drug treatment (Fig. 4G), indicating that only the free drug administration caused obvious toxicity at the current drug dose.

(A) Schematic chart of the treatment schedule. (B) Bioluminescence images of lung metastasis at different time points. EXP indicates Expired. (C) Lung metastasis progression curve as depicted from in vivo bioluminescence signal intensity. (D) Quantification of lung metastasis burden at different time points (n = 7). (E) Scatter plot comparing the lung metastasis burden in different treatment groups as depicted from bioluminescence signal intensity on day 16 (n = 7). Significantly different (Kruskal-Wallis test): *P < 0.05, **P < 0.01, and ****P < 0.0001. (F) Scatter plot comparison of the lung metastasis burden on day 23 (n = 7). Significantly different (Kruskal-Wallis test): *P < 0.05, **P < 0.01, and ****P < 0.0001. (G) Body weight change of mice during the treatment period (n = 7). (H) Survival of mice under different treatments as displayed by Kaplan-Meier curves (n = 7). Significantly different (log-rank test): *P < 0.05 and ***P < 0.001.

Next, we investigated the antimetastatic activity of the developed therapies in late-stage lung metastasis. As shown in Fig. 5A, after intravenous tumor cell injection, mice received four doses of therapies every other day with the first dose being administered a week after inoculation (day 7). According to the bioluminescence images (Fig. 5B) and lung metastasis growth curve (Fig. 5C) of individual mice, using the drug NPs alone did not lead to significant inhibition of lung metastasis progression. However, the drug NPs assembled on erythrocytes (ELeCt) were able to slow down the lung metastasis progression, although not as notably as in the early-stage metastasis model. The overall lung metastasis burden data shown in Fig. 5D confirmed the better efficacy of the hitchhiked drug NPs over using the free NPs alone. In particular, on day 16 after tumor inoculation, the hitchhiked drug NPs exhibited a 2.4-fold better efficacy in terms of inhibiting metastasis growth. On day 16, the lungs were excised, and the surface metastatic nodules on the lungs were counted. The surface nodules data shown in Fig. 5E were consistent with the bioluminescence metastasis burden data evaluated with bioluminescence. A 2.3-fold better efficacy in reducing surface nodules was achieved by assembling the drug NPs to the erythrocytes. The hematoxylin and eosin (H&E) analysis of the lungs of mice confirmed this result (fig. S1). In addition, the body weight change data shown in Fig. 2F and the H &E analysis data shown in fig. S2 suggested that no significant toxicity was associated with any of the treatments. We then conducted a separate study to evaluate the efficacy of the therapies in terms of extending the animal survival time. As shown in Fig. 5G, unlike in the early-stage metastasis model, the use of drug NPs alone did not provide any survival benefit. However, the treatment using drug NPs assembled on erythrocytes (ELeCt) significantly improved the animal survival, extending the median survival time from 28.5 to 37 days. In particular, one of eight mice that received the hitchhiked drug NPs continued to survive for at least 48 days.

(A) Schematic illustration of the treatment schedule. (B) Bioluminescence images of lung metastasis progression at different time points. (C) Lung metastasis growth curve in mice treated with different DOX formulations. (D) Quantitative analysis of lung metastasis burden as depicted from bioluminescence signal intensity (n = 7). Significantly different (one-way ANOVA): *P < 0.05 and **P < 0.01. (E) Quantification of metastasis nodule numbers on excised lungs from mice in different treatment groups on day 16 (n = 7). Significantly different (one-way ANOVA): **P < 0.01 and ***P < 0.001. (F) Body weight change of mice during the treatment period (n = 7). (G) Kaplan-Meier survival curves of mice in different treatment groups. Significantly different (log-rank test): **P < 0.01 and ***P < 0.001.

To test the feasibility of using the ELeCt platform for the delivery of other chemotherapeutic agents, we selected six other common chemotherapeutic agents or their combinations, including camptothecin, paclitaxel, docetaxel, 5-fluorouracil, gemcitabine, methotrexate, and the combination of 5-fluorouracil and methotrexate, and loaded them into the biodegradable PLGA NPs. Despite having diverse physicochemical properties (shown in fig. S3 and table S1), the different chemotherapeutic agentloaded NPs were able to assemble onto erythrocytes (Fig. 6). These data supported that the biodegradable drug NP assembling onto erythrocytes approach (ELeCt) can potentially be a versatile platform to deliver selected chemotherapies to lung metastasis that originated from different primary tumors.

The tested chemotherapeutic agents include camptothecin, paclitaxel, docetaxel, 5-fluorouracil, gemcitabine, methotrexate, and the combination of 5-fluorouracil and methotrexate. Scale bars, 1 m.

Because of its unique physiological features like high blood throughput and high density of narrow capillaries, lung is one of the major organs into which the evaded tumor cells from primary tumor sites can spread (31). Patients with advanced cancer (30 to 55%) have lung metastasis (32). Treating lung metastasis is more challenging than treating the primary tumors because it typically progresses more aggressively (33). Systemic chemotherapy is one standard treatment option for lung metastasis. However, its efficacy is usually far from desirable, attributed to its ineffective targeting and poor accumulation in the lungs. Conventional NP-mediated drug delivery also fails to achieve good localization with the desired site of action (34). Here, we report an erythrocyte hitchhiking platform, ELeCt, consisting of drug-loaded biodegradable NPs assembled on erythrocytes for promoting chemotherapy for effective lung metastasis treatment. Excellent studies have shown NPs hitchhiking on erythrocytes to accumulate in lungs, including recently in metastatic lungs (35); however, the ability of such a mechanism to yield survival benefits has not been known. To that end, we successfully demonstrate the ability of ELeCt to slow down the progression and improve the survival in early- and late-stage experimental melanoma metastasis models, resembling early detection and mid-to-late detection clinical scenarios, respectively.

Conventional nanomedicines use the attachment of active targeting ligands to enhance the targeted delivery of chemotherapeutic payloads (10, 11, 3640). The ELeCt platform developed in this work exploits a completely new paradigm, taking advantage of the unique physiology of the target sites (high shear stress) and responsive dislodging of the chemotherapeutic payloads. Our in vitro drug-release data showed that the biodegradable NPs were able to have burst followed by relatively sustained drug release. Our pharmacokinetic and biodistribution data suggested that the ELeCt platform has two important features compared with the free drug and NPs aloneextended blood circulation time and improved accumulation to lung metastasis. Actually, both features are favorable for lung metastasis treatment. The extended circulation time is consistent with previous reports (27, 41). By hitchhiking to erythrocytes, NPs experience less immune recognition by the reticuloendothelial system organs, enabling them to stay in circulation for a longer time (27, 28, 41). The higher concentration of payload drug in the blood endowed by the ELeCt would allow more drug to interact with and kill the circulating tumor cells. Our in vitro shear study data evidently proved that the detachment of drug NPs from erythrocytes is shear dependent, and this is the basis for using the platform to precisely deliver payload chemotherapeutics to the target lung metastasis sites. It should be noticed that a substantial portion of the drug NPs were also detached at the low shear stress. This factor emphasized the need for investigating the surface modification of the drug NPs to modulate the binding strength of drug NPs to erythrocytes for future explorations with this technology. Our biodistribution data suggested that the biodegradable NP assembly on erythrocyte (ELeCt) platform was able to deliver a high concentration of payload chemotherapeutics to the lung metastatic sites in a short period of time. Impressively, the ELeCt platform delivered 16.6-fold more drug to the lungs bearing metastasis in 20 min compared with using the drug NPs alone. In comparison, the conventional targeted nanomedicine approach using targeting ligands can rarely achieve such high delivery enhancement (17, 42). Moreover, it usually shows a maximum tumor accumulation at a significantly longer time point (12 to 24 hours), depending on the properties of the nanomedicine (43). The quick and targeted delivery of drug NPs by the ELeCt platform would bring benefits for inhibiting tumor growth. For instance, typical nanomedicines, independent of their material origins, usually have an initial burst drug release and thus cause premature drug leakage (44), potentially attenuating the therapeutic efficacy and often leading to toxicity. The quick and targeted delivery achieved by the ELeCt platform has the potential to circumvent this issue. In addition, not unexpectedly, the lung section imaging suggested that the deposited NPs were distributed throughout the lung sections, both the inside and the outside of the lung metastatic nodules. The NPs deposited outside of the metastasis nodules have the potential to serve as a drug reservoir to release drug that can relocate to the metastatic nodules within close proximity.

Our in vivo efficacy data suggested that the enhanced and targeted delivery of chemotherapeutics by the ELeCt platform could bring benefits for inhibiting both the early-stage and the late-stage lung metastasis growth. In the early-stage lung metastasis model, the treatments using free drug or drug NPs alone exhibited some slowdown of the progression of lung metastasis. However, their antimetastatic efficacy was not potent enough to significantly extend the animal survival. In comparison, the ELeCt platform was able to provide a 100- to 300-fold better antimetastatic efficacy compared with using the free drug or drug NPs alone. Its improved antimetastatic efficacy led to a significantly extended animal survival, extending the median survival time of mice bearing lung metastasis by 32 days, compared with the control group. The data suggested that the ELeCt platform has the potential to enable chemotherapy for effective treatment of early-stage lung metastasis. In the late-stage metastasis model, the administration of drug NPs alone failed to significantly inhibit the lung metastasis growth and to improve the survival time. The ELeCt platform was able to significantly slow down the lung metastasis progression and modestly improved animal survival. Evidently, the antimetastatic efficacy of the therapies is closely related to the start time of the therapies. The efficacy of the developed therapies to treat in an even later-stage lung metastasis has not been shown yet. In addition, future studies may also need to be done to unveil the effect of drug dose and schedule of the therapies on their antimetastatic efficacy.

The exact mechanism of the drug-loaded biodegradable NP assembling on erythrocytes is not clear. Previous studies from our laboratory and others have attributed the assembly of NPs to erythrocytes to the noncovalent interactions such as electrostatic interactions, hydrophobic interactions, and H-bonds between the polymeric NPs and domains on the red blood cell (RBC) membrane (27, 28, 35). The assembly is most likely a result of balance between surface tension forces caused by the NP-induced membrane stretching and the noncovalent interactions between the cell membrane and NPs. The balance of the two factors drives stable assembly of the particles onto erythrocytes (27). However, details of this mechanism need future investigation. Our drug NP binding data suggested that the model drugloaded NPs, in this case, DOX, could assemble onto the mouse erythrocytes at a very high binding efficiency. This feature is critical for making the ELeCt platform work. The number of erythrocytes that can be administered has an upper limit, and only having a high drug dose on individual erythrocytes can achieve the therapeutic concentration of chemotherapeutics. In addition, our data also suggested that the drug dose on erythrocytes could be tuned by changing the feed incubation ratios of drug NPs to erythrocytes, thus providing the possibility of changing drug dosage according to specific lung metastasis conditions. Other than DOX, we were able to load different commonly used chemotherapeutic agents or their combinations to the biodegradable NPs. Moreover, these drug-loaded NPs could assemble onto the mouse erythrocytes as well. This opens a new window to use the ELeCt platform to treat lung metastasis originating from different primary sites. Lung metastasis can have different primary tumor origins like breast cancer, bladder cancer, melanoma, and many others. The metastasis derived from different origins is preferably treated by specific chemotherapeutic agents (45, 46). The ELeCt platform has the potential to be a versatile platform to treat different lung metastasis by loading optimal chemotherapeutic agents according to their primary tumor origins. The impact of the chemotherapeutics properties on the performance of the ELeCt platform should be further investigated in future studies. Our data also suggested that the drug-loaded biodegradable NPs efficiently assembled onto human erythrocytes and were detached from them under lung-corresponding shear stress. In addition, the material used to prepare the biodegradable NPs (PLGA) is part of several FDA-approved products (47). Therefore, this platform technology has a translational potential. However, this needs to be explored further in the future.

In summary, the ELeCt platform, drug-loaded biodegradable NP assembling on erythrocyte, was developed, which enables lung physiologyassisted shear-responsive targeted delivery of chemotherapeutic agents to treat lung metastasis. The drug NPs assembled on erythrocytes could be precisely dislodged in the lungs bearing metastasis in response to the intrinsic mechanical high shear stress. Various commonly used chemotherapeutic agents could be loaded into the biodegradable NPs and further made to successfully assemble onto the erythrocytes. This platform successfully delivered one-order-of-magnitude-higher content of the model drug (DOX) to the diseased lungs as compared with using the NPs alone. This platform enabled chemotherapy to effectively inhibit lung metastasis growth and significantly improve the survival. All in all, the ELeCt platform can be a versatile strategy to treat lung metastasis originating from different primary tumors, with a strong translational potential.

PLGA NPs encapsulating DOX were prepared using a nanoprecipitation method. Briefly, 5 mg of DOX was dissolved in 500 l of methanol and 5 l of triethylamine. This was added to 1 ml of acetone containing 20 mg of PLGA. The mixture was then injected into 10 ml of 1% polyvinyl alcohol solution under constant stirring using a syringe pump at 1 ml/min. The particles were kept under constant stirring overnight before removing the organic solvents using rotary evaporation. The formed particles were centrifuged at 12,000g for 15 min, and the supernatant was analyzed to quantify drug loading. The particles were then resuspended in deionized water and assessed for their size, zeta potential, and polydispersity index using dynamic light scattering (Malvern Zen3600) and SEM (Zeiss FESEM Supra 55VP, Zeiss FESEM Ultra 55). The NPs were washed for a total of two washes with deionized water before their final resuspension in phosphate-buffered saline (PBS). Nanoparticles containing other chemotherapeutic drugs were prepared using the similar nanoprecipitation technique described above with minor modifications (details are shown in the Supplementary Materials).

Murine whole blood was collected via cardiac puncture using a heparin precoated syringe and stored in BD Microtainer blood collection tubes prior to use. Whole blood was centrifuged at 1000g for 10 min at 4C to remove the serum and the buffy coat layers from the erythrocyte compartment. The isolated erythrocytes were further washed three times with cold PBS and centrifuged at 650g for 15 min at 4C before their final resuspension at a concentration of 10% hematocrit in PBS (erythrocyte stock solution). Human whole blood obtained from BioIVT (NY, USA) was processed and stored using the same procedure as murine blood. Freshly processed erythrocytes were used for every experiment in this study.

Equal volumes of erythrocyte stock solution and drug NP suspension were mixed in Axygen 1.5-ml Self-Standing Screw Cap Tubes and further thoroughly mixed by inversion and pipetting. The tubes were then allowed to rotate on a tube revolver (Thermo Fisher Scientific) for 40 min. The hitchhiked erythrocytes were then pelleted by centrifugation at 100g for 5 min at 4C, unabsorbed particles were carefully removed, and the pellet was washed again with 1 ml of 1 PBS to remove loosely bound particles. The hitchhiked erythrocytes were finally resuspended at 10% (v/v) in 1 PBS and used for further characterization or in vivo studies.

Hitchhiking efficiency and the drug loading on erythrocytes were determined using fluorescence measurements. For quantification using fluorescence, 25 l of erythrocytes was lysed using deionized water, and the drug content was quantified using DOX fluorescence [excitation (Ex)/emission (Em), 470/590 nm] on a plate reader (Tecan Safire 2, NC, USA). The percentage of erythrocytes carrying NPs for different NP-to-erythrocyte ratios was determined using flow cytometry (BD LSR Analyzer II, CA, USA) using DOX fluorescence (Em/Ex, 470/590 nm) and confirmed by confocal microscopy (Upright Zeiss LSM 710 NLO ready, Germany). Nanoparticle assembly to erythrocytes was confirmed using SEM (Zeiss FESEM Supra 55VP, Zeiss FESEM Ultra 55). Briefly, the hitchhiked erythrocytes were fixed using 2.5% glutaraldehyde solution and washed in an increasing ethanol gradient before being chemically dried using hexamethyldisilazane. Last, the samples were sputter coated (EMT 150T ES metal sputter coater, PA, USA) prior to imaging.

For serum stability studies, hitchhiked murine and human erythrocytes were incubated in 1 ml of fetal bovine serum (FBS) or human serum (from BioIVT) on a tube revolver at 12 rpm at 37C. These conditions simulate low shear physiological environment. After incubation for 20 min, the cells were pelleted by centrifugation at 250g for 5 min and resuspended to 10% (v/v) in 1 PBS. Twenty-five microliters of erythrocytes was then lysed using deionized water, and the remaining drug content was quantified using DOX fluorescence (Ex/Em, 470/590 nm) on a plate reader (Tecan Safire 2).

For shear studies, hitchhiked murine and human erythrocytes were incubated in 10 ml of FBS or human serum. A rotatory shear (6 Pa) was applied to erythrocytes in serum using a cylindrical coquette viscometer (1 mm gap, AR-G2 rheometer, TA instruments, DE, USA) for 20 min. The samples were maintained at 37C during the application of shear using a water jacket. These conditions simulate lung-corresponding high shear physiological environment. After 20 min, the cells were pelleted by centrifugation at 250g for 10 min and resuspended to 10% (v/v) in 1 PBS. Twenty-five microliters of erythrocytes was then lysed using deionized water, and the remaining drug content was quantified using DOX fluorescence (Ex/Em, 470/590 nm) on a plate reader (Tecan Safire 2).

Female C57BL/6 mice (7 to 9 weeks of age) were purchased from Charles River Laboratories (MA, USA). All experiments were performed according to the approved protocols by the Institutional Animal Care and Use Committee of the Faculty of Arts and Sciences, Harvard University, Cambridge.

For the pharmacokinetics study, healthy female C57BL/6 mice were used. Free DOX, DOX-loaded NPs, and drug NPs assembled on erythrocytes (RBC-NPs) (n = 3 for all groups) were injected intravenously into the tail vein at a dose of 5.2 mg/kg. Blood samples were collected from the mice by submandibular bleed at 2 min, 15 min, 30 min, 2 hours, and 5 hours after the injection. The plasma was separated from the cellular component by centrifuging at 5000 rpm for 10 min. DOX was extracted from both the compartments (30 l) using 150 l of acetonitrile. The drug content was quantified using reversed-phase liquid chromatographymass spectroscopy (LC-MS; Agilent 1290/6140 UHPLC, CA, USA) ran through an Agilent C-18 column (Poroshell 120, EC-C18, 3.0 mm by 100 mm, 2.7 m) using a gradient mobile solvent.

For the biodistribution studies, 1 105 B16F10-Luc cells were injected intravenously into the tail vein of female C57BL/6 mice. Fourteen days after inoculation, mice were intravenously injected with free DOX, DOX-loaded NPs, and drug NPs assembled on erythrocytes (RBC-NPs) (n = 3 for all groups) into the tail vein at a dose of 5.2 mg/kg. Mice were euthanized at 20 min and 6 hours after the injection, and organs were harvested for further processing. Organs were rinsed using cold PBS three times to remove the residual blood. One milliliter of cold deionized water was added to each organ, and the organs were homogenized using a high shear homogenizer (IKA T 10 Basic ULTRA-TURRAX, NC, USA). DOX was extracted from the homogenates using acetonitrile (1:4 homogenate:acetonitrile), and the drug content was quantified using DOX fluorescence (Em/Ex, 470/590 nm) on a plate reader (Tecan Safire 2). The data are expressed as drug content (micrograms) normalized to the organ weight.

For NP distribution within the diseased lungs, 1 105 B16F10-Luc cells were injected intravenously into the tail vein of female C57BL/6 mice. Twenty-eight days after inoculation, mice were injected with DOX-loaded NPs and drug NPs assembled on erythrocytes (RBC-NPs). Twenty minutes after the injection, the mice were euthanized, and the intact lungs were collected. Lungs were washed twice with cold 1 PBS before being fixed in a 4% paraformaldehyde solution overnight. The fixed lungs were then frozen in Tissue-Tek OCT compound (Sakura Finetek) and sectioned using a cryostat (Leica CM1950, IL, USA). The sectioned tissue was mounted using Fluroshield to stain for DAPI (4,6-diamidino-2-phenylindole) (Ex/Em, 340/488 nm) and was analyzed using a confocal microscope (Upright Zeiss LSM 710 NLO ready).

An experimental lung metastasis model was established by intravenous injection of 1 105 B16F10-Luc cells into the tail vein of female C57BL/6 mice. Efficacy for the treatment groups was evaluated in early-stage and late-stage metastatic models. Mice were randomized on the basis of the bioluminescence intensity in the lungs 1 day before the first injection of therapies. A control (saline) group and three treatment groups (DOX-NPs, RBC-NPs, and free DOX) at a dose of 5.2 mg/kg were evaluated for their efficacy (n = 7 for all groups, unless otherwise specified).

For the early-stage metastatic model, treatments were given starting the day after the inoculation. Four injections were given over 6 days, i.e., days 1, 3, 5, and 7 after inoculation. On days 6, 8, 10, 12,18, 23, and 31 after inoculation, the mice were imaged using in vivo imaging (PerkinElmer IVIS Spectrum, MA, USA). Briefly, mice were injected intraperitoneally with 150 l of XenoLight-d-luciferin (30 mg/ml) in saline. Fifteen minutes after the injection, mice were imaged using in vivo imaging. The average radiance (bioluminescence intensity) was evaluated using the software Living system. The animals were further monitored for their survival.

For the late-stage metastatic model, treatments were given 1 week after the inoculation. Four injections were given over 6 days, i.e., days 7, 9, 11, and 13 after the inoculation. The mice were imaged on days 6, 8, 10, 12, and 16 using in vivo imaging as described above. The average radiance was evaluated using the software Living system. On day 16, the mice were euthanized, and the lungs were excised and fixed using 10% formalin. The fixed lungs were used for counting of the surface nodules and H&E analysis. Survival in the late-stage model was evaluated by having the injection schedule as described above (n = 8 for the control and treatment groups).

All data are presented as means SEM. Comparison between two groups was conducted using unpaired two-tailed Students t test. Comparisons among multiple groups were conducted using one-way analysis of variance (ANOVA) or Kruskal-Wallis test. Kruskal-Wallis tests were performed for data that were determined to be nonparametric by the normality test. All statistical analyses were carried out using GraphPad Prism 8 software. For the analysis of Kaplan-Meier survival curves, log-rank (Mantel-Cox) analysis was used. P values represent different levels of significance: *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. All the flow cytometry analyses were carried out using the FlowJo software.

Supplementary material for this article is available at http://advances.sciencemag.org/cgi/content/full/5/11/eaax9250/DC1

Supplementary Materials and Methods

Fig. S1. Representative H&E staining images of lungs of mice.

Fig. S2. Representative H&E staining images of organs of mice treated with different drug formulations.

Fig. S3. Size distribution of different chemotherapeutic agentloaded biodegradable PLGA NPs.

Table S1. Physicochemical properties of different chemotherapeutic agentloaded biodegradable PLGA NPs.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

Acknowledgments: Funding: This work was financially supported by Wyss Institute at Harvard University. We acknowledge funding from NIH (1R01HL143806-01). Author contributions: Z.Z., A.U., and S.M. conceived the project. Z.Z. and A.U. performed the experiments. Y.G. and J.K. helped with the LC-MS and histology analysis. Z.Z. and A.U. analyzed the data. Z.Z. prepared the graphs. Z.Z., A.U., and S.M. wrote the manuscript. All authors read and approved the manuscript. Competing interests: S.M., A.U., and Z.Z. are inventors on a patent application related to this work filed by Harvard University (no. 62/858,478, filed in June 2019). The authors declare that they have no other competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.

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Erythrocyte leveraged chemotherapy (ELeCt): Nanoparticle assembly on erythrocyte surface to combat lung metastasis - Science Advances

Fruits of youth: Consider this your guide to an anti-aging diet – Iosco County News Herald

There are several well-known factors that contribute to aging: smoking, stress, sun exposure and genetics, among them. But what you may not realize is what you put on your plate can also cause you to look beyond your year.

Everything that goes into your mouth affects your aging cycle, says Tina Martini, chef and author of "Delicious Medicine: The Healing Power of Food." Among the offenders are alcohol, refined sugar, and over-processed and fried foods.

When you eat fresh foods, as close to nature as you can get, you can slow down the effects of damage to your cells, thus slowing the aging process, she says.

Fried Foods

One of the main damaging factors is acrylamide, an organic compound that is produced during the frying process of some starchy foods. This has led the American Cancer Society to recommend limiting French fries, potato chips, foods made from grains breakfast cereals, cookies, toast because they tend to have higher levels of this chemical.

Acrylamide can affect the skin in the same way that sun damage does, says Martini.

Reduce your exposure by soaking raw potato slices in water for 15 to 30 minutes and draining before frying or roasting. Also, when cooking, choose boiling or steaming to stop acrylamide from forming.

Refined Sugar

Sugar is the main source of energy for our bodies, but it can also contribute to a process called glycation. The excess sugar molecules attach to proteins, creating "advanced glycation end products" or AGEs, which are linked to the loss of collagen. Losing collagen can lead to wrinkles and creepy skin.

Remove processed sugary foods from your diet and eat fresh vegetables and fruits, says Lorraine Kearney, BASc, NDTR, an adjunct lecturer at the City University of New York. To get the maximum nutrients from fruits enjoy them whole instead of dried, blended or pureed.

Snack on organic peaches, cherries, apples and strawberries as they can help to fight the signs of aging.

Enriched and Fortified Foods

You will often find the words enriched and fortified on labels of prepackaged foods. Those words generally mean that all the nutrients were removed during processing, and the manufacturer put back what they deemed important. A diet high in processed foods can lead to oxidative stress, which occurs due to an imbalance of free radicals and antioxidants in your body and may lead to cell damage.

Eating a diet rich in colorful foods increases the amount of nutrients the body needs to heal on the cellular level, Kearney says.

Stock up on fresh and antioxidant-rich foods like blackberries, blueberries, beets, bell peppers and radishes as they mitigate the effects of oxidative stress.

Alcohol

While drinking some alcohols in moderation is known to have beneficial health effects, overuse can cause wrinkles, puffiness, inflammation and dehydration.

While you should always remain hydrated, be extra mindful when consuming spirits; and instead of having a full glass of wine, enjoy a spritzer made with seltzer. Staying hydrated not only keeps your skin supple but also flushes toxins from the body.

Skin care is very important in protecting our skin, but its really the moisture in our bodies that keeps us radiant, says Martini.

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Fruits of youth: Consider this your guide to an anti-aging diet - Iosco County News Herald

SANGHA Retreat by OCTAVE Institute Announces 21 New Wellness Programs – Benzinga

SANGHA Retreat by OCTAVE Institute announces 21 new wellness programs to help guide guests on their personal journeys of awakening to achieve mind, body and spiritual wellness.

SUZHOU, China (PRWEB) November 12, 2019

SANGHA Retreat by OCTAVE Institute announces 21 new wellness programs* to help guide guests on their personal journeys of awakening to achieve mind, body and spiritual wellness. Ranging from two to seven nights, each program includes:

The themes of the programs are:

Ancient Chinese Wisdom

(4 days/3 nights or 7 days/6 nights): Experience deep healing through acupuncture, traditional Chinese medicine, and seasonal nutrition, incorporating more than 2000 years of Chinese medical wisdom combined with Western science.

Traditional Spinal Alignment

(4 days/3 nights or 7 days/6 nights): Customized vertebral therapy corrects stress, tension, poor posture and inflammation in conjunction with rehab coaches.

Seasonal Gut Management

(4 days/3 nights or 7 days/6 nights): Significantly improve health--including your immune system, allergies, fatigue -- through the ancient Chinese practice of eating seasonally.

Relax and Reconnect

(4 days/3 nights or 7 days/6 nights): Destress, reconnect with your self and your life path, and learn to be at one with your environment.

Age Renewal

(4 days/3 nights or 7 days/6 nights): Our anti-aging doctor teaches how to promote inner health for outer beauty, to look and feel younger.

Sleep Recovery

(7 days/6 nights): Discover the roots of sleep disruption through traditional Chinese medicine, Sleep Lab study, and massage.

Welcome to Wellbeing

(2 days/1 night or 4 days/3 nights): The perfect add-on to an Asian trip, this health awareness program helps you to unwind and restore positive energy to welcome abundance.

Cleanse and Reset

(7 days/6 nights): Improve the digestive system through traditional Chinese medicine and scientific testing, combined with a cleansing diet.

Fearless Fitness

(7 days/6 nights): Gain mental strength and understand the exercises that best suit you in this active retreat for optimal fitness performance.

Mindful Weight Management

(7 days/6 nights): Break unhealthy habits with food and learn to eat mindfully with the help of a nutritionist and life coach.

Heart to Heart

(7 days/6 nights): Designed to enhance cardiology health.

Men 360

(7 days/6 nights): A medical-focused retreat to improve overall health through traditional medicine, bioenergetic science, and naturopathy.

Women 360

(7 days/6 nights): A medical-focused retreat designed for a woman's unique physical and emotional wellbeing.

Ayurvedic Healing Journey

(4 days/3 nights or 7 days/6 nights): Instill lifestyle changes through the ancient practice of Ayurveda and Yoga to reboot and prevent future health conditions.

*For additional information or to book a program, please visit the link below (first select dates, then click check availability' to view programs: http://www.sangharetreat.com/booking

SANGHA Retreat by OCTAVE Institute

Located west of Shanghai on the outskirts of Suzhou, China, SANGHA Retreat combines Eastern philosophies backed by Western science to help people awaken to a life of greater purpose and personal wellbeing. With a focus on raising consciousness, it has been created for the need of our time by visionary Frederick Chavalit Tsao, fourth-generation steward of a family business that started in The Shanghai Bund before 1906.

The 47-acre retreat, designed by Tsao & McKown Architects, includes AT ONE guest suites; AT ONE Clinic, offering comprehensive wellness assessments and wellbeing programs; AT ONE Healing Spa with extensive spa treatment menu and hydrotherapy circuit; THOUGHT FOR FOOD restaurant; the Meditation Dome; and a seasonal schedule of fitness activities and mindfulness classes designed around the body's circadian rhythm.

For more information and reservations, visit sangharetreat.com. Reservations can also be made through Hidden Doorways Travel or a preferred travel agent.

OCTAVE Institute

More than a school of life, OCTAVE Institute is a platform to help people find clarity, harmony and a new level of consciousness and freedom. It is comprised of SANGHA Retreat, THE VILLAGE, and AITIA in Suzhou; and THE LIVING ROOM, an urban support center in Shanghai. THE VILLAGE is a live-work-learn community for personal and communal learning, executive retreats, conferences, summits and festivals including the AT ONE International Festival, an annual, international festival that brings together thought leaders and holistic practitioners to address current issues. AITIA powers the communal programs at OCTAVE Institute. Together they create the foundation for growth towards a purposeful life, mindfully lived. http://www.octaveinstitute.com/

Media Contacts:

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For the original version on PRWeb visit: https://www.prweb.com/releases/sangha_retreat_by_octave_institute_announces_21_new_wellness_programs/prweb16714379.htm

Read more here:
SANGHA Retreat by OCTAVE Institute Announces 21 New Wellness Programs - Benzinga