French professional tennis player Gilles Simon seems quite fascinated with Roger Federer. Even when he doesnt want to talk about the Swiss Maestro, journalists find a way to ask him something about Federer. However, he respects the 20-time Grand Slam winner and has admitted earlier that Federer was his sons hero.

In a recent interview, there was a question about the former World No.3 Grigor Dimitrov. However, the interviewer phrased it in a manner that it also involved Federer. The question was whether Dimitrov had limited himself in his career by replicating Federer. To this, the Frenchman replied in affirmative.

Yes, began a confident Simon. Hes (Grigor Dimitrov) clearly a player who comes close in terms of fluidity of play.

It was clear that Simon felt that Dimitrov was quite close in cloning Roger Federers style. He further believed that it was something that was not doing any good for Dimitrov.

Read More: Gilles Simon racks up Roger Federer again in the debate on tennis role model

Simon further argued that Dimitrov was trying to do something that he simply couldnt do.

In the important moments, it can give up because, in reality, it is not entirely in his DNA, concluded the 35-year-old Simon.

He was blunt and didnt hesitate in saying that Federers style was not there in Dimitrovs DNA. He said that the player could try to copy it but it was futile as it would not help him in the longer run.

It was certain that Simon was criticizing Dimitrov. In a way, he even advised him not to do so in the future.

Both Dimitrov and Simon will begin the upcoming season at the Australian Open 2021. Joining them there will be none other than Roger Federer who will also make a comeback to tennis after a year.

Also Read: Gilles Simon slams French Tennis for wasting energy on replicating Roger Federer

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Not Entirely in His DNA: Gilles Simon Criticizes Grigor Dimitrov for Cloning the Style of Ro ... - EssentiallySports

Rick and Morty season 4's introduction of Space Beth raised the question of which Beth is a clone, and we think Space Beth is the real one.

Rick and Morty season 4brought the original Beth and clone Beth face to face, and even though no one in the show knows who is the real Beth, there is reason to believe "Space Beth" is the real one. The idea of cloning Beth came duringRick and Morty season 3. Beth was in the middle of a breakup with Jerry and sought adventure, but she put her future in Rick's hands.However, it wasn't until season 4 thatRick and Morty provided any exploration of what happened.

Two versions of Beth were featured inRick and Morty's season 4 finale, "Star Mort Rickturn of the Jerri." After a full season of Beth reunited with her family, Space Beth arrived as a cosmic warrior who spent her time battling the New Galactic Federation. Space Beth believed that she wasa clone after she discovered an implant in her neck,prompting her to return back to Earth to kill Rick. However, she learns that both Beths have such implants, adding to the confusion of who is real and who is a clone. Although both Beths were interested in learning who was real and who was the clone at first,Rick and Morty ended season 4with the entire family, except Rick, left in the dark. He begins toreveal his "Mind Blower" of the event (a recorded memory), which shows him cloning Beth, but then removing the labels from the cloning vats and rotate their placements repeatedly so he wouldn't know which Beth was real.

Related: Rick & Morty Theory: Beth Is Smarter Than Rick

SinceRick and Morty didn't provide any answers on which Beth is real and which is a clone,a fanbase built on wild theories has been left to create some more. The truth behind the two versions of Beth is one of the topics that has continued to be debated since season 4 ended, with cases to be made for either Beth being the clone. Since they were exactly the same at the end of the cloning process and Beth's uncertainty about what she wanted,the future for both Beths could just come down to where Rick put them.That said, one of them is definitively a clone, and even if Rick and Morty never answers the question,there's reason tobelieve that Space Beth is the real, or at least original Beth.

Space Beth's trajectory as a character falls in line with what Beth wanted for herself, based on her discussion about cloning with Rick. Sheexpressed a desireto go have more adventures after spending time in Froopyland with Rick and realizing that she's more like her father than she thought. Space Beth being the real Beth means she finally got the chance to break free of her mundane life and find happiness elsewhere. Although this would mean that the real Beth also abandoned her family, it was already fractured at this time. This arc gives Beth some agency in her story, instead of simply coming to a realization that she was happy all along and just didn't realize it. Plus, it's not like this would be the first time a Sanchez left their family behind to pursue their own interests, so this reveal would further illustrate how similar Beth and Rick are.

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Rick & Morty Theory: Space Beth Is Real Beth | Screen Rant - Screen Rant

Jurassic Park's science uses frog DNA is a notable addition to the incomplete genome of a dinosaur, and it also gives them very special abilities.

The originalJurassic Parkfilmreveals that frog DNA was used to help create the dinosaurs, causing a problem with InGen's plan to limit breeding. Released in 1993, Steven Spielberg's classic not onlyboastedground-breaking advancements inCGI technology, but it also explored thescientific phenomena of cloning. While the logistics are not fully explained in the movie, they still provide a compellinglookinto the reemergence of dinosaurs.

When paleontologist Dr. Alan Grantand the team first arrive at the Jurassic Park Visitor Center, they are treated to a tour by John Hammond, founder of the park and of InGen, the company which makes dinosaur cloning possible. Their first stop includes a filmnarrated by Mr. DNA, the animated helix, who explains how the dinosaurs came to be. In simplified terms, scientists were able to extract dinosaur blood from prehistoric mosquitos preserved in amber. Because most of the samples' genomes were incomplete, geneticists needed something to, as Mr. DNA says, "fill in the holes and complete the code." This is where frog DNAcamein.

Related: Jurassic Park: All 6 Dinosaurs That Appear In The First Movie Explained

Frog DNA serves as both an easy, uncomplicated solution to the dinosaurs' genetic sequence and a plot device for later on in the film. It's that DNA solution that is responsible foreverydinosaur in the Jurassic Park series, like the T-Rex and Velociraptor before the later movies move into more overt genetic splicing. It'scombinedwith the dinosaurs' DNA sequence to make a finalized version for fertilization, filling in the gaps from degradation over the thousands of years since their extinction. In the film, it's only frog DNA that was used to serve the plot and explain how some dinosaurs have been able to change sex, while the original book uses a number of additional options. Fundamentally, it serves two other purposes too. Not only does it foreshadow the franchise's genetic tinkering and establishes that Dr Henry Wu's morals come second to his scientific drive,while adding an accessible means to explain how the amber extraction method can possibly lead to full sequence DNA cloning.

Michael Crichton's novel, on which the movie is based, provides a complex analysis of the differentchicken and amphibian genomes used in creating and boosting the growth of dinosaurs.Jurassic Park'sfilmmakers likely wanted to makethe process as simple as possible for audiences to understand, sofrog DNAbecame the sole additionalinsertion into the dinosaurs' biological makeup. Frog DNAis mentioned again in a later scene, when Dr. Grant, Lex, and Tim,discover freshly-hatched eggs outside of the lab. According to Dr. Wu, the head geneticist of the park's lab, all dinosaurs are programmed to be female, so breeding should be impossible. Upon finding the eggs, Dr. Grant remarks that some West African frogscan alter their sex in a single-sex space.Bymarrying a frogs genetic code with the dinosaurs', scientists gave dinosaursthe frogs ability to change sex and, therefore, mate. However, what type of frog DNA was actually used and whether the scientists knew about this trait from their research remains unclear.

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Jurassic Park: Why Frog DNA Was Used to Create The Dinosaurs - Screen Rant

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More time at home means many Australians now have the opportunity to re-assess their lifestyle and spending habits and set some serious saving goals. So how can you actually go about it?

In this episode of The nestegg Podcast, host Grace Ormsby is joined by Melbourne-based Reventons Billie Christofi to talk all things finance and the impact of isolation on our hip pockets.

In stage 4 lockdown, Billie shares the importance of going back to basics when it comes to budgeting, methods to re-assess spending habits, and some of the less conventional ways people can boost their wealth at this time.

Thanks Billie, for sharing your insights with the nestegg podcast team!

Have an investment story to share?Get in touch at[emailprotected]and you might get featured on an upcoming episode!

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Critical steps outlined with property transfers and trust cloning | nestegg - nestegg.com.au

Introduction: Global Hard Drive Cloning Software Market, 2020-26

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Vendor Landscape

AcronisMacrium ReflectTodo BackupDAEMON ToolsMiniToolNovaBACKUPClonezillaAOMEI BackupperIperius BackupActive Disk ImageOandO DiskImageAOMEI Image Deploy

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Large EnterprisesSMEs

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Global Hard Drive Cloning Software Market Expected To Reach Highest CAGR By 2026: Acronis, Macrium Reflect, Todo Backup, DAEMON Tools, MiniTool etc. -...

The colt is a clone of a male Przewalski's horse and the first successful cloning of the species, San Diego Zoo officials said in a news release on September 4. It was born August 6 to a domestic surrogate mother. Przewalski's horse are known as the last wild horse, according to the National Zoo. They were originally native to Europe and Asia, but the expansion of humans and environmental changes depleted their numbers.

Scientists said that the horse was formally extinct in the wild, and has been surviving for the last 40 years primarily in zoos. Some herds have been found in Mongolia.

"This birth expands the opportunity for genetic rescue of endangered wild species," said Ryan Phelan, executive director of Revive & Restore, a wildlife conservation organization promoting biotechnologies that's partnering with San Diego Zoo Global and ViaGen Equine on the cloning project.

"Advanced reproductive technologies, including cloning, can save species by allowing us to restore genetic diversity that would have otherwise been lost to time."

The DNA used for the colt was cryopreserved in 1980 at the San Diego Zoo Global (SDZG) Frozen Zoo. The cloned stallion was born in 1975 in the United Kingdom and was transferred to the US in 1978. The zoo said he lived until 1998.

"As the new clone matures and successfully breeds, he can provide a valuable infusion of genetic diversity for the Przewalski's horse population," the news release said.

The baby horse was named "Kurt," in honor of Kurt Benirschke, who was instrumental in founding the Frozen Zoo and the conservation research program at San Diego Zoo Global.

Kurt will be moved to the San Diego Zoo Safari Park when he is older to be integrated into a breeding species.

"This colt is expected to be one of the most genetically important individuals of his species," said Bob Wiese, chief life sciences officer at San Diego Zoo Global.

"We are hopeful that he will bring back genetic variation important for the future of the Przewalski's horse population."

More here:

A clone of the endangered Przewalski's horse is born of DNA saved for 40 years - CNN

The colt represents the first time this species has been cloned, and scientists indicate it could provide an important model for future conservation efforts.

The birth of Kurt means there is hope for endangered wild species, said Dr. Veneklasen, rzewalski horses were close to extinct in the early 1900s when there were less than 30 in the world. The wild population is estimated to be around 2,000 nowadays, all lacking genetic diversity.

The new cloned foal, who will be moved to the San Diego Zoo Safari Park to be integrated into a breeding herd of his species once he is older, represents a major milestone for Przewalskis horse conservation. He was cloned from a cell line stored in the Frozen Zoo since 1980. That stallion was born in 1975 in the UK, was transferred to the US in 1978 and lived until 1998. As the new clone matures and successfully breeds, he can provide a valuable infusion of genetic diversity for the Przewalskis horse population.

This colt is expected to be one of the most genetically important individuals of his species, said Bob Wiese Ph.D., chief life sciences officer at San Diego Zoo Global. We are hopeful that he will bring back genetic variation important for the future of the Przewalskis horse population.

The colt has been named Kurt, in honor of Kurt Benirschke, M.D., who was instrumental in founding the Frozen Zoo and the conservation research program at San Diego Zoo Global.Kurt was born fully healthy and reproductively normal.

Formerly extinct in the wild, the Przewalskis horse has survived for the past 40 years almost entirely in zoos around the world, and all of the surviving horses are related to 12 Przewalskis horses born in the wild. Intensive breeding programs have helped recover the species, yet losses of genetic diversity have occurredbut those losses can now be halted, using cellular technologies such as cloning, based on the availability of living cells stored in the Frozen Zoo.

While ongoing reintroductions since the 1990s have established several wild herds on grasslands in China and Mongolia,maintaining genetic variation is likely to be an important part of ensuring the species survival in the future.

Advanced reproductive technologies are relatively standard for domestic horses and cattle. However, there have been few attempts to work with endangered species. The successful birth of this foal demonstrates how these techniques can be used for conservation efforts, today and into the future.

Timber Creek Veterinary has worked with ViaGen Equine for over 15 years, foaling hundreds of cloned horses for clients around the world.

Przewalski's Facts from Wikipedia:

Przewalski's horse is stockily built in comparison to domesticated horses, with shorter legs.

Typical height is about 1214hands(4856inches, 122142cm), length is about 2.1m (6ft 11in).

It weighs around 300 kilograms (660lb).

The coat is generallydunin color withpangarfeatures, varying from dark brown around the mane (which stands erect) to pale brown on the flanks and yellowish-white on the belly and around the muzzle.

The legs of Przewalski's horse are often faintly striped, also typical ofprimitive markings.

The tail is about 90cm (35.43in) long, with a longerdockand shorter hair than seen in domesticated horses.

The hooves of Przewalski's horse are longer in the front and have significantly thicker sole horns than feral horses. This is beneficial, as it improves the performance of the hooves.

The Przewalski's horse has 66 chromosomes, compared to 64 in all other horse species.

More:

Meet Dr. Gregg Veneklasen, the vet behind the worlds only cloned Przewalski horse - Jill Lopez

SAN DIEGO, Calif. - About 70 companies worldwide are working on therapies for COVID-19 using cloned antibodies, according to an estimate by the International AIDS Vaccine Initiative. A few of these treatments, known officially as monoclonal antibody therapies, have advanced to clinical trials.

Most require an injection, but San Diego-based Diomics and its partner Active Motif, based in Carlsbad, are developing a once-a-day spray that could be easily self-administered.

Early research suggests the coronavirus primarily enters the body through the nose. The spray, called Dioguard, is designed to coat the lining of the nasal cavity with cloned antibodies that are held in place for 24 hours or more using a proprietary polymer material developed by Diomics.

Diomics CEO Anthony Zolezzi said he believes the spray holds the key to allowing many aspects of life to resume until the day comes when theres an effective vaccine in widespread use.

Diomics is also developing two tests for COVID-19 antibodies using its polymer beads, including a device that looks like a nicotine patch that is designed to monitor for infections for a week or more.

Active Motif is providing the cloned antibodies for the nasal spray. With a lab in Shanghai and other relationships in China, the Carlsbad company was able to clone antibodies from 11 Chinese survivors in February, before the World Health Organization declared a pandemic.

When the company started the cloning project, they thought the virus would probably disappear in three to six months, said Active Motif CEO Ted DeFrank. Then people started realizing, no this is going to be with us for a while.

The plasma from the 11 Chinese patients contained thousands of antibodies, and scientists with Active Motif set about selecting the one that was most effective, dubbed 414-1. The company says it can neutralize SARS-CoV-2 virus particles with 98 percent effectiveness.

Monoclonal antibodies have some similarities to convalescent plasma, which is a complex cocktail of antibodies and other immune molecules drawn from the blood of recovered patients. One of the key differences is that cloned antibodies can be mass produced in a lab.

Cloned antibodies have been used in treatments for more than 30 years, primarily for cancer. One such treatment famously helped former U.S. President Jimmy Carter beat melanoma.

But of the more than 100 monoclonal antibody therapies licensed for use, only seven are for communicable diseases, according to IAVI.

Historically, the treatments have been expensive and difficult to produce, but Diomics said its targeting a price of about $2 a spray for Dioguard, roughly $45 per bottle.

We do not want to have huge profits from a pandemic, thats just wrong, Zolezzi said. Were going to price this as effectively as we can for the masses. We want to get this out to the masses.

Animal testing is about to begin and the companies said they hope to progress to human trials soon. Their goal is to release the spray in early 2021, when a vaccine may be on the market but not yet widely available.

If the spray works, it could be adapted to other viruses to help fight future pandemics, Zolezzi said.

"Thats our real goal," Zolezzi said. "That we never get caught flat-footed like this time."

This story was first reported by Derek Staahl at KGTV in San Diego, California.

See the article here:

Biotechs in California developing COVID-blocking nasal spray with cloned antibodies - kjrh.com

The railways have decided to let 20 pairs of clone trains run on busy routes, these trains will run on busy routes. The Tclone trains will run from September 21. These cloning trains are fully reserved and run at pre-arranged times. Railways have decided to run clone trains to eliminate the mess of waiting tickets. The full list of these cloned trains has been released.

The train number of the cloned trains running through the railway will be the same as the main train number. The speed of these cloned trains will be higher than the main trains. At the same time, the stops of these trains will also be less than those of the main train, that is, these trains will stop at limited stations. At the same time, the main train and its cloned train will arrive at the last station at approximately the same time. Reservations on these trains start from September 19.

The fare of the cloned trains is the same as the main train. According to information from the Ministry of Railways, of the 20 pairs of clone trains, 19 pairs of clone trains will run in the Humsafar class, the fare of which will also be the same as the Humsafar train. At the same time, 1 train pair will be operated as Janashtabdi Express from Lucknow Delhi, whose fare will be equal to Janashtabdi Express. These trains have a reservation period of 10 days.

The list of cloned trains issued by the railway includes several trains for Bihar. According to the list of railway cloned trains, train number (02563) will run from Saharsa to New Delhi. The Clone train (02564) runs daily from New Delhi to Saharsa. Apart from this, there will also be a lot of clone trains running to Darbhanga, Muzaffarpur, Rajgir and Rajendranagar stations of Bihar. Apart from this, trains will run from Amritsar-Jayanagar, Bangalore-Danapur, Ahmedabad-Darbhanga, Patna-Ahmedabad.

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Indian Railways: 20 pairs of clone trains running from September 21st, no waiting tickets, see full list of trains here. IRCTC last update: Indian...

| 09/10/2020 | ionicons-v5-c10: 16 | Francisco Trejo |

San Diego.- Scientists from the zoo of San Diego succeeded in cloning the first horse of Przewalski, a horse endangered wild native to the steppes of central Asia.

Breeding horse was baptized as Kurt and now symbolizes hope for his species that was on the brink of extinction.

Which makes that Kurt even more exciting is that it was cloned from genetic material cryopreserved 40 years ago, reviving genetic diversity that was believed to have been lost decades ago.

This foal is expected to be one of the most genetically important individuals of its species, said zoologist Bob Wiese, director of biological sciences at San Diego Zoo Global.

We are hopeful that it will recover important genetic variation for the future of the Przewalski horse population.

The last confirmed sighting of a horse of Przewalski in the wild it was in 1969.

Fortunately, some of the horses still remained in zoos. But not many. A total of 12 horses formed the ancestors of a captive breeding program: 11 horses of Przewalski captured in freedom between 1899 and 1902, and another captured in 1947. Thanks to this breeding program, today there are about 2,000 specimens.

(With information from Science Alert)

Originally posted here:

Heir to the first domestic horse is cloned - Explica

The first successfully cloned endangered Przewalskis horse was born on Aug. 6 in a veterinary facility in Texas, San Diego Zoo Global announced on Friday. The horse was cloned from DNA of a male Przewalskis horse cryopreserved by the zoo in 1980.

Przewalskis horses are critically endangered animals that are found in Mongolia, per Smithsonians National Zoo. Theyre considered the last species of truly wild horses and are distant cousins of modern day domestic horses, having likely split from a common ancestor around 500,000 years ago, per the Smithsonian.

Przewalskis horses were once extinct in the wild, and while intensive breeding programs helped revive the species and reintroduce them into the grasslands of China and Mongolia, nearly all can be traced back to 12 Przewalskis horses that were born in the wild, the San Diego Zoo said in its press release. The successful cloning of DNA collected 40 years is meant to introduce key generic diversity into the species that could benefit its survival. The zoo said the cloned Przewalskis horse will eventually be transferred to the San Diego Zoo Safari Park and integrated into a herd of other Przewalskis horses for breeding.

The work to save endangered species requires collaborative and dedicated partners with aligned goals, Paul A. Baribault, president of San Diego Zoo Global, said in a statement. We share in this remarkable achievement because we applied our multidisciplinary approach, working with the best scientific minds and utilizing precious genetic material collected and stored in our wildlife DNA bio bank.

The cloned Przewalskis horse was named Kurt after Dr. Kurt Benirschke, the creator of the San Diego Zoo Global Frozen Zoo, which has been collecting and preserving genetic material of endangered animals since 1975.

San Diego Zoo Global partnered with Revive & Restorea wildlife conservation group that aims to incorporate biotechnology into conservation effortsand ViaGen Equinea company that clones horses and petsto successfully clone the Przewalskis horse.

This birth expands the opportunity for genetic rescue of endangered wild species, said Ryan Phelan, executive director of Revive & Restore, in a statement. Advanced reproductive technologies, including cloning, can save species by allowing us to restore genetic diversity that would have otherwise been lost to time.

Przewalskis horses are not the only species Revive & Restore is trying to revive via biotechnology. The group is attempting to revive at least six endangered or extinct species, including the Wooly Mammoth, which went extinct around 4,000 years ago. Other organizations and research facilities are also attempting to revive extinct or endangered species with biotechnology, including the International Islamic University Malaysia, which plans to revive the recently extinct Sumatran rhino.

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Write to Madeleine Carlisle at madeleine.carlisle@time.com.

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First Clone of Endangered Przewalskis Horse Born in Conservation Effort to Save the Species - TIME

By Erik Gudris

The US Open is known for its often raucous, vocal fans who make themselves heard everywhere.

But when it was announced that this years US Open would not have spectators on site, many tennis watchers wondered how that might impact not only the players, but the overall experience of the event.

More: Ashe Experience Special for Tsitsipas, Auger-Aliassime

Tournament organizers turned to IBM to help them provide a solutionAI (artificial intelligence) generated crowd noise. Using technology called AI Sounds, engineers digested video from last years US Open and then ranked the excitement level of various video clips. These clips were then turned into a reel and then classified to create a crowd reaction score.

From a player hitting an ace, to saving a break point, or winning a match, all these various sounds were then made available to in-stadium and television production crews covering this years event to use the sounds depending on whats happening in a match during real-time.

COVID-19 brought disruption to sports as a whole, and the ability of fans to experience live sporting events has been heavily impacted in 2020. At the same time, the pandemic accelerated the need for engaging technologies using AI and underpinned by a scalable hybrid cloud," said Noah Syken, Vice President of Sports & Entertainment Partnerships, IBM. "As the technology partner to the USTA, we transformed our offerings to meet tennis fans where they are this yearexperiencing the sport through the US Open digital properties everywhere.

These sounds are definitely noticeable in matches being played at the site two biggest courtsArthur Ashe Stadium and Louis Armstrong Stadium. Without them, it sometimes feels like the players are competing inside an empty parking lot, rather than inside one of the sports most prestigious stages.

Using digital sounds in sporting events is nothing new; even before the current pandemic prompted many sports leagues to not allow fans at their respective matches or games.

According to the New York Times, 20 years ago CBS used taped nature sounds during a PGA golf event that drew criticism from bird experts over the use of such sounds. Nowadays, the practice is more common.

European football leagues when they returned to action this summer are employing virtual stadium noises created for FIFA video games. The National Football League (NFL) is considering using artificial crowd sounds during football games this fall.

Getting back to tennis, fans watching at home are divided over the non-natural sounds being used in New York. Some fans like that it adds some atmosphere for them as they cheer on their favorite pros. Others dont like the artificial sounds and would prefer experiencing the court as it is.

For the players themselves, although they would prefer to have fans on-site, not having fans this year is just one more thing they have to adjust to during this very different U.S. Open.

It's digital fans. It's 2020, Serena Williams said when asked about not having fans on site. Im all about the future. I'm glad we're able to have the Open.

"It's really cool that we can have it. It's so important that this is justwe don't know what the new normal is going to be. So it's just good that we can have virtual fans and be able to have sport.

Photo credit: Andrew Ong/USTA

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Cloning Crescendo: US Open Computer Crowd Noise Fills The Void of No Fans - Tennis Now

Why chase after an ideal or a lost love? Each love is unique and cannot be cloned, no matter what!

You can never love any two or more people in the same manner and with the same intensity. Love is an emotion of a myriad hues, never experienced in exactly the same way by different people. And never again even by the same two people in an unchanged form. This is because love is dependent on so many variable factors that it cannot possibly be replicated.

The love that two people share stems from their individual needs, mutual chemistry, and also from their own capacity to give and receive the emotion. It is dependent on individual personal characteristics, and each persons experiences and unique circumstances also have a deep bearing on the outcome. This accounts for the rather changeable nature of love.

Not all loves, nor indeed all relationships, can feed the same need in you. Physical need is just one aspect; we also have emotional needs, spiritual and intellectual needs, and some needs that we ourselves may not understand. Hence you get attracted to other people and at any given time, could theoretically have loving relationships with multiple people. Needs vary, and so a relationship based on need, would vary too.

The only people who can love everyone in exactly the same manner, repeating the same experiences, are almost certainly psychopaths, or at least sociopaths, using their bookish understanding of the emotion to exploit others. In short, these are people just acting out the emotion.

We waste a lifetime in the impossible pursuit of an ideal love or the recreation of a love we once had and lost but cloning is not part of the Love DNA! You cannot ever experience the love of a Romeo and Juliet however hard you try, because that love was the product of their circumstances and the interplay of their individual characteristics and chemistry at a certain time in history, placed in a unique geographical setting. How can you possibly love like them, when you are not them? Heck, even if Romeo and Juliet were themselves to step out of Shakespeares pages, with changed variables, they cannot possibly love the same way again! This time round they might end up happily married, growing old together or splitting paths along the way!

You can certainly love several times in your lifetime, sometimes simultaneously too, but never ever in the exact same manner, clocking the same experiences and emotion. That is impossible because we change and evolve as we go along in life, and our needs and dependencies change too. Time and experience teach us better control of our emotions, patience, forgiveness and self-sufficiency. Conversely, bad experiences at love and relationships may lead to bitterness and cynicism in some.

So, if people change so much and so do their needs, how then can our experience of love remain the same? Love changes too. And that is what makes it so precious, so unique to each. Why look for a clone when you can create your own unique love?

DISCLAIMER : Views expressed above are the author's own.

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Can love be replicated? - The Times of India Blog

Persistence Market Research recently published a market study that sheds light on the growth prospects of the global Fosmid Cloning market during the forecast period (20XX-20XX). In addition, the report also includes a detailed analysis of the impact of the novel COVID-19 pandemic on the future prospects of the Fosmid Cloning market. The report provides a thorough evaluation of the latest trends, market drivers, opportunities, and challenges within the global Fosmid Cloning market to assist our clients arrive at beneficial business decisions.

The recent published research report sheds light on critical aspects of the global Fosmid Cloning market such as vendor landscape, competitive strategies, market drivers and challenges along with the regional analysis. The report helps the readers to draw a suitable conclusion and clearly understand the current and future scenario and trends of global Fosmid Cloning market. The research study comes out as a compilation of useful guidelines for players to understand and define their strategies more efficiently in order to keep themselves ahead of their competitors. The report profiles leading companies of the global Fosmid Cloning market along with the emerging new ventures who are creating an impact on the global market with their latest innovations and technologies.

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Global Fosmid Cloning Market by Companies:

The company profile section of the report offers great insights such as market revenue and market share of global Fosmid Cloning market. Key companies listed in the report are:

Key Participants

The key participants in fosmid cloning Market are Bio S&T, Illumina, Inc., Lucigen, and others. The companies are mainly focusing on intense marketing to convey benefits of fosmid cloning.

The report covers exhaustive analysis on:

Regional analysis for Market includes

Report Highlights:

Global Fosmid Cloning Market by Geography:

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Some of the Major Highlights of TOC covers in Fosmid Cloning Market Report:

Chapter 1: Methodology & Scope of Fosmid Cloning Market

Chapter 2: Executive Summary of Fosmid Cloning Market

Chapter 3: Fosmid Cloning Industry Insights

Chapter 4: Fosmid Cloning Market, By Region

Chapter 5: Company Profile

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Growing Focus on Product Innovation Likely to Impact the Growth of the Fosmid Cloning Market 2017 2025 - StartupNG

John Boyega may have walked away disappointed with his experience working on the new Star Wars trilogy, but that hasnt turned him off sci-fi in general. The actor is slated to star in the Netflix original sci-fi mystery movie They Cloned Tyrone, and hes finally getting some company in front of the camera. Jamie Foxx and Teyonah Parris (Chi-raq, Candyman) have joined the cast of the movie.

The Hollywood Reporter has news on the They Cloned Tyrone cast expanding with Jamie Foxx and Teyonah Parris, but it hasnt been specified who their characters are. John Boyega is leading the film, and it seems that these two new additions to the cast will fill out the trio at the center of the movie who investigates a series of eerie events, alerting them to a nefarious conspiracy lurking directly beneath their hood. We dont know the entire conspiracy, but were betting it has something to do with cloning someone named Tyrone.

They Cloned Tyrone will mark the directorial debut of Creed II writer Juel Taylor, who co-wrote the script for this new movie with Tony Rettenmaier. Theyll both also be producing with Charles King and his Macro production banner, along with Stephen Dr. Love and Kim Roth. Poppy Hanks and Mark R. Wright are also executive producing for Macro.

Back in 2019, They Cloned Tyrone was one of the films named on The Black List, a collection of the best unproduced screedplays in Hollywood. Thankfully, Netflix snatched up the movie that has been described as a pulpy, sci-fi mystery caper that is like a combination of Friday and Get Out.

John Boyega has already shown his strengths in comedy and sci-fi action thanks to Attack the Block and the Star Wars franchise. Foxx is just coming off another Netflix sci-fi movie, Project Power, and we know he can handle comedy very well. And finally Teyonah Parris has proven to be quite the diverse actress too, with supporting roles in Mad Men, They Came Together, If Beale Street Could Talk, and Empire. Plus, shes also playing a grown Monica Rambeau in Marvels upcoming WandaVision series on Disney+. So having all three of them together for a pulpy movie like this should be something worth watching whenever it arrives on Netflix.

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'They Cloned Tyrone' Adds Jamie Foxx and Teyonah Parris to the Netflix Sci-Fi Mystery - /FILM

Global Voice Cloning Market Report from AMA Research highlights deep analysis on market characteristics, sizing, estimates and growth by segmentation, regional breakdowns& country along with competitive landscape, players market shares, and strategies that are key in the market. The exploration provides a 360 view and insights, highlighting major outcomes of the industry. These insights help the business decision-makers to formulate better business plans and make informed decisions to improved profitability. In addition, the study helps venture or private players in understanding the companies in more detail to make better informed decisions.

Major Players in This Report Include,

Google (United States), Microsoft (United States), IBM (United States), AWS (United States), AT&T (United States), Baidu (China), Nuance Communications (United States), iSpeech (United States), NeoSpeech (United States), CereProc (Scotland), Cepstral (United States), Lyrebird (Canada), Kata.ai (Indonesia), Alt.ai (Japan), Aristech GmbH (Germany), Acapela Group (Belgium), VocaliD (United States), Voicery (United States) and Voctro Labs (Spain).

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Definition

Voice cloning, is a technique that helps in audiobook narration, highly desired feature for personalized speech interfaces. It is based on copying and reusing of recorded voice or speech with the help of cloning software. In the future, Artificial Intelligence powered voice cloning technology provides enhanced conversational ability of voice assistants and chatbots. Of late, according to Chinese internet giant Baidus AI research team, revealed that AI software can learn and replicate the way a person speaks and communicates with it. Such a positive technological advancement is the major driving agent for the voice cloning market globally

Global Voice Cloning Market Report offers a detailed overview of this market and discusses the dominant factors affecting the growth of the market. The impact of Porters five armies on the market over the next few years has been discussed for a long time in this study. We will also forecast global market size and market outlook over the next few years.

Types of Products, Applications and Global Voice Cloning Market Report Geographical Scope taken as the Main Parameter for Market Analysis. This Research Report Conducts an assessment of the industry chain supporting this market. It also provides accurate information on various aspects of this market, such as production capacity, available production capacity utilization, industrial policies affecting the manufacturing chain and market growth.

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Market Drivers

Market Trend

Restraints

Opportunities

The Global Voice Cloning is segmented by following Product Types:

by Application (Accessibility, Chatbots and assistants, Digital games, Interactive games, Others), Component Type (Solutions (Software Tools, Platforms), Services (Managed Services, Professional Services)), Verticals Type (Healthcare and life sciences, Education, Media and entertainment, Telecom, Travel and hospitality, Banking, Financial Services, and Insurance (BFSI), Others), Deployment Model (Cloud, On-premises)

.

In this research study, the prime factors that are impelling the growth of the Global Voice Cloning market report have been studied thoroughly in a bid to estimate the overall value and the size of this market by the end of the forecast period. The impact of the driving forces, limitations, challenges, and opportunities has been examined extensively. The key trends that manage the interest of the customers have also been interpreted accurately for the benefit of the readers.

The Voice Cloning market study is being classified by Type, Applicationsand major geographies with country level break-up that includes South America (Brazil, Argentina, Rest of South America), Asia Pacific (China, Japan, India, South Korea, Taiwan, Australia, Rest of Asia-Pacific), Europe (Germany, France, Italy, United Kingdom, Netherlands, Rest of Europe), MEA (Middle East, Africa), North America (United States, Canada, Mexico).

The report concludes with in-depth details on the business operations and financial structure of leading vendors in the Global Voice Cloning market report, Overview of Key trends in the past and present are in reports that are reported to be beneficial for companies looking for venture businesses in this market. Information about the various marketing channels and well-known distributors in this market was also provided here. This study serves as a rich guide for established players and new players in this market.

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Current Scenario Analysis for Decision Framework

Key Strategic Developments in Voice Cloning Market:

The research includes the key strategic activities such as Research & Development (R&D) initiatives, Merger & Acquisition (M&A) completed, agreements, new launches, collaborations, partnerships & (JV) Joint ventures, and regional growth of the key competitors operating in the market at global and regional scale to overcome current slowdown due to COVID-19.

Key Market Features in Global Voice Cloning Market

The report highlights Voice Cloning market features, including revenue size, weighted average regional price, capacity utilization rate, production rate, gross margins, consumption, import & export, demand & supply, cost bench-marking in Voice Cloning market share and annualized growth rate (Y-o-Y) and Periodic CAGR.

Extracts from Table of Contents

Global Voice Cloning Market Research Report

Chapter 1 Global Voice Cloning Market Overview

Chapter 2 Global Economic Impact on Industry

Chapter 3 Global Market Competition by Manufacturers

Chapter 4 Global Revenue (Value, Volume*) by Region

Chapter 5 Global Supplies (Production), Consumption, Export, Import by Regions

Chapter 6 Global Revenue (Value, Volume*), Price* Trend by Type

Chapter 7 Global Market Analysis by Application

.continued

This report also analyzes the regulatory framework of the Global Markets Voice Cloning Market Report to inform stakeholders about the various norms, regulations, this can have an impact. It also collects in-depth information from the detailed primary and secondary research techniques analyzed using the most efficient analysis tools. Based on the statistics gained from this systematic study, market research provides estimates for market participants and readers.

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Voice Cloning Market Expected to Boost the Global Industry Growth in the Near Future - The News Brok

Tahlequah has plenty of medical marijuana dispensaries for those with state medical cards, but patients could save money in the long run and enjoy the fruits, or buds, of their labor by growing their own.

A patient with an Oklahoma Medical Marijuana Authority license can legally possess six mature marijuana plants, and six seedling plants. The start up for those new to "gardening" depends on how much cannabis they would like to grow, and which method they would like to use. A grower could plant seeds or clone indoors using the traditional soil method, or could take the hydroponic route by growing it in a reservoir of water - or take the clone or seeds and throw them in the dirt in the backyard.

Jackie Guinn, owner of Jus Grow Me in Tahlequah, said she would start beginners with a few pots, lights to use inside their homes, and soil for under $500. However, if people want to take it further, they could get a setup that costs upward of $1,000.

"The price is going to depend on how many plants you're going to do," said Guinn. "It depends on what lights you want to get. I've got lights that start at $130 and on up. I would probably start them off with a little clone in a 1-gallon pot."

Whether it's a potted plant or using the hydroponic method, Guinn said a grower should give the plant 18 hours of light and six hours of darkness while it is in its vegetative state. She would water it about every other day and give it a variety of nutrients. Once the plant reaches about knee high, it can be transplanted into a 5-gallon bucket.

"You would keep that in its vegetative state until it reaches about 4 feet high," said Guinn. "When it starts to flower, it's going to double in size, and if you're growing indoors, that's kind of hard for most people."

Once a plant reaches about 4 feet, the grower should transition it to its flowering stage. That requires a different set of nutrients, which would help the plant yield more buds and avoid starvation.

"When you go into flowering, you need your blooming nutrients to help produce the buds, the THC, the CBDs, and your trichomes," said Guinn. "Those are always important, because the higher CBD and THC are going to eliminate your pain."

Miracle-Gro should not be used on cannabis. It contains to much nitrogen, which would give the plant issues once it reaches flowering stage. Guinn said another factor is making sure the plant's water has the appropriate PH balance.

"Tap water is the worst for your plants," she said. "It can burn them up. The PH needs to be between 6.5 and 7. If you use distilled water, you've got to make sure you add Cal-Mag, because distilled water does not have it and your plants need that. Rain water is the best for them."

Those who plant their marijuana outside should make sure the plants don't get too much water, or else they will be susceptible to root rot. Guinn recommends raisers a tarp to cover the plants if it rains too much.

Growers need to look out for mites and other bugs. There are a variety of organic pesticides or other natural ways of getting rid of pests, such as using lady bugs. Cultivators should also be on the lookout for mold and mildew.

"If that happens, you need to just throw them all away. It's not healthy to make any kind of medicine out of that at all," said Guinn. "You've got to keep your humidity in your tent or your room just right. That's a very important step, because if the humidity gets too high, it can cause mold or mildew on your plants."

After the plant reaches flowering stage, the grower can start to harvest anywhere from 60 to 90 days. It depends on what he would like to get out of the plant. Guinn said examining the plant's trichomes, which are small resin glands found on the flowers, will help one decide when it's time to harvest.

"Your trichomes will start out [in the flowering stage] as clear," said Guinn. "Then, it goes to cloudy, and then it can go to ember. When it goes to cloudy, you can harvest. It's higher in THC when it's cloudy. You get higher CBD when it turns to the ember color. The trichomes are the little crystals all over your leaves."

Jus Grow Me offers free classes for people looking to start their own small grow operations. Students can learn about cloning, hydroponics, starting a plant in soil and more. While it may seem complicated at first, Guinn said it takes little time to get the hang of it.

"Every day check on your plant," she said. "Once you start growing your plant, it gets pretty easy. You get to know your plant and you can tell if you have a deficiency. All they've got to do is call me and we'll get them through this. We're all about people growing their own natural medicine. It's the way to go."

Learn more

To find out more information about growing cannabis or classes at Jus Grow Me, call 918-822-3634.

We are making critical coverage of the coronavirus available for free. Please consider subscribing so we can continue to bring you the latest news and information on this developing story.

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5Ws+1H: How It's Done: Card holders can save money by growing their own - Tahlequah Daily Press

Comprehensive quantitative and qualitative high-end research on the Voice Cloning Market. According to the current market state, this report has been prepared in accordance with continuous observation of the global market. This report has been formulated to give our clients the most up to date data on the Voice Cloning Market.

Top Companies are covering This Report:-

IBMGoogleLyrebirdNuance CommunicationsBaiduMicrosoftAWSAT&TNeoSpeechSmartbox Assistive TechnologyexCloneLumenVoxKata.AiAlt.AiCereProcAcapela GroupVocaliDVoiceryAristechCepstral

Reports Intellect projects Voice Cloning Market based on elite players, present, past, and forecast data for the coming years which will act as a profitable guide for all the market competitors. The study includes growth trends, micro- economic and macro-economic indicators in detail and the report has been assessed with the help of PESTEL analysis and other essential analyses operating in the Voice Cloning Market.

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The report offers all the essential data for players to secure a position of strength in the market all while creating a comprehensive action plan. Our analysts here at Reports Intellect have used advanced primary and secondary research techniques to create the most up to date assessment of data on the Voice Cloning Market which opens up a plethora of new opportunities to create new strategies to gain leverage over the competition.

Product Type SegmentationCloudOn-premises

Industry SegmentationHealthcare and life sciencesEducationMedia and entertainmentTelecomTravel and hospitality

Market Segment by Regions, regional analysis covers

North America (United States, Canada, Mexico)

Asia-Pacific (China, Japan, Korea, India, Southeast Asia)

South America (Brazil, Argentina, Colombia, etc.)

Europe, Middle East and Africa

Germany, France, UK, Russia and Italy, Saudi Arabia, UAE, Egypt, Nigeria, South Africa

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Voice Cloning Market Future Growth Analysis, Business Demand and Opportunities to 2026 | Google, Lyrebird, Nuance Communications, Baidu, Microsoft -...

Global Voice Cloning Market was value US$ 460Mn in 2018 and is expected to reach US$ 1,812Mn by 2026 at a CAGR of 18.69%.Global Voice Cloning Market, by Deployment ModeVoice cloning is a highly preferred feature for personalized speech interfaces. Speaker coding is based on training a separate model to directly assume a new speaker embedding from cloning audios and to be used with a multi-speaker propagative model.

Increasing in the introduction of new initiatives in voice cloning market will further push voice cloning market trends in the upcoming years. Though, complexities in recreating natural speech will obstruct the growth of the market during forecast period. But, integration of artificial intelligence technologies with voice cloning personalization and services in human-equipment interaction will offer profitable avenues for the players across the voice cloning market in the nearby future.

Cloud deployment mode segment is predicted to grow at a higher CAGR over the forecast period. Many vendors have started offering cloud-based voice cloning market. The demand for cloud-based voice cloning deployment mode is projected to grow due to the various benefits offered by the cloud, for instance, scalability, flexibility, and cost-effectiveness.

Chatbots and assistants are becoming vital to delivering a seamless experience & are becoming fundamental in conversational interfaces. So, vendors around the globe are focusing on enhancing the conversational capabilities of chatbots and assistants by integrating the AI-powered voice cloning technology market. Voice cloning solutions would aid enterprises to add voice cloning capabilities to make chatbots or assistants sound more natural.

Region-wise, North America is dominating the global voice cloning market. An enormous number of vendors and industry participants in North America are involved in new product development and product innovation. These vendors are also aiming at integrating AI capabilities into voice cloning solutions to develop more natural sounding voice cloning samples. Reputed manufacturers are founding new units for the production of consumer electronics goods using voice cloning technologies. Many research initiatives across the region are predictable to fuel the adoption of voice cloning solutions across some business verticals and domains.

Global voice cloning market report includes PESTLE analysis, competitive landscape, and Porters five force model. Market attractive analysis wherein all the segments are benchmarked based on the market size, growth rate, and general attractiveness.

Key players operating in the global voice cloning market, Google, Microsoft, IBM Corporation, AT&T Corp., Baidu, Nuance Communications, CereProc.Scope of Global Voice Cloning Market

Global Voice Cloning Market, by Component

Solutionso Software Toolso Platforms ServicesGlobal Voice Cloning Market, by Application

Chatbots & Assistants Accessibility Digital Games Interactive learningGlobal Voice Cloning Market, by Deployment Mode

Cloud On-PremisesGlobal Voice Cloning Market, by Region

North America Europe Asia Pacific Middle East and Africa South AmericaKey players operating in Global Voice Cloning Market

Google Microsoft IBM AWS AT&T Baidu Nuance Communications iSpeech NeoSpeech CereProc Cepstral# Lyrebird Kata.ai Alt Inc. Aristech GmbH Acapela Group VocaliD Voicer Voctro Labs exClone CandyVoice LumenVox rSpeak Smartbox Assistive Technolog VivoTex.

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Global Voice Cloning Market : Industry Analysis and Forecast (2019-2026) by Component, Application, Deployment mode, and Region. - Galus Australis

Vida Mirzaie,1 Touba Eslaminejad,2 Homayoon Babaei,3 Seyed Noureddin Nematollahi-Mahani4,5

1Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; 2Pharmaceutics Research Centre, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; 3Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran; 4Neuroscience Research Centre, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; 5Afzal Research Institute (NGO), Kerman University of Medical Sciences, Kerman, Iran

Correspondence: Seyed Noureddin Nematollahi-Mahani Tel +983 433257343Email nnematollahi@kmu.ac.ir

Purpose: Human butyrylcholinesterase (BChE) serves as a bio scavenger to counteract organophosphate poisoning. It is also a potential drug candidate in several therapeutic fields. Therefore, in the present study, we constructed a new dual-promoter plasmid consisting of Cytomegalovirus (CMV) and human elongation factor 1 (EF-1) promoters and transfected that into HEK-293 cells using Lipofectamine to enhance the BChE secretion.Methods: The new dual-promoter construction (pBudCE dual BChE) including two copies of the BChE gene was designed and transfected into cells by liposomal structures. The cloned plasmids were evaluated by enzyme digestion and gel electrophoresis analysis. Experimental groups were categorized into the cells transfected by pBudCE dual BChE (treatment), pCMV (positive control) vectors, and nontransfected cells (negative control). BChE gene expression was evaluated by qRT-PCR and the enzyme activity was assessed using modified Ellmans method. The freeze-thaw process was carried out for analyzing the stability of the pBudCE dual BChE vector.Results: Validation examination of the cloned plasmids confirmed the successful cloning process. The gene expression level and Ellmans method value in pBudCE dual BChE was higher than the other groups. CMV promoter has also increased the enzyme activity, although the difference was not significant compared with the control group. Interestingly, freeze-thaw cycles followed by several passages did not affect the enzyme activity.Conclusion: The designed construction with CMV and EF-1 promoters could increase BChE gene expression and the activity of the BChE enzyme in HEK-293 cell line. Large-scale production of BChE enzyme can be achieved by using dual-promoter plasmid construction compared to a single-promoter vector to be used in clinical trials.

Keywords: dual-promoter, vector, recombinant protein, drug delivery, Ellmans method, HEK-293

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License.By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

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Enhancing the Butyrylcholinesterase Activity in HEK-293 Cell Line by D | DDDT - Dove Medical Press