1 person injured in planned demolition of US 95 bridge – Las Vegas Review-Journal

A U.S. 95 bridge collapsed while under construction Thursday afternoon, briefly trapping and injuring a worker, authorities said.

A Nevada Department of Transportation project had involved a planned demolishment of a bridge, and had closed Eastern Avenue, fire, transportation and highway officials said. Shortly before 4 p.m., authorities received a report of a collapse in the area and that a person was trapped.

The injured contract worker complained of back pain, and workplace safety officials were quickly notified, according to officials.

The construction worker was taken to a nearby hospital, and treated for injuriies.

We do not have details yet as to the extent of those injuries and are working to get updates in real time, said Justin Hopkins, Transportation Department spokesman.

Spokeswoman Adrienne Packer added that the collapse was not due to any structural deficiencies and that the freeway was not damaged. She said the collapse occurred as crews brought down an old girder that buckled.

The workers injuries were very minor, according to Packer.

Transportation Department officials said they did not believe crews did anything wrong, but Packer said she expected an Occupational Safety and Health Administration investigation into the collapse.

Crews have been demolishing the southbound section of the Eastern Avenue overpass as part of its $40 million U.S. 95 viaduct replacement and retrofit work.

Eastern has been closed to vehicle and pedestrians traffic under that bridge since Monday and traffic on U.S. 95 has been shifted.

The road had been scheduled to reopen late Tuesday night. U.S. Highway 95 and Interstate 515 remained open and safe for motorists Thursday night, according to a Transportation Department press release.

NDOT is closely monitoring the situation and working with safety officials to determine the cause of the incident, the release stated.

Contact David Wilson at dwilson@reviewjournal.com. Follow @davidwilson_RJ on Twitter.

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1 person injured in planned demolition of US 95 bridge - Las Vegas Review-Journal

Austrian Nazism – Wikipedia

Political party in Austria

Austrian Nazism or Austrian National Socialism was a pan-German movement that was formed at the beginning of the 20th century. The movement took a concrete form on 15 November 1903 when the German Worker's Party (DAP) was established in Austria with its secretariat stationed in the town of Aussig (now st nad Labem in the Czech Republic). It was suppressed under the rule of Engelbert Dollfuss (193234), with its political organization, the DNSAP ("German National Socialist Workers' Party") banned in early 1933, but revived and made part of the German Nazi Party after the German annexation of Austria in 1938.[14]

Franko Stein, from the town of Eger (now Cheb, Czech Republic) and an apprentice bookbinder Ludwig Vogel, from the town of Brx (now Most, Czech Republic), organised the Deutschnationaler Arbeiterbund (German National Workers' League) in 1893. It was a collection of laborers, apprentices, and trade unionists from the railroads, mines, and textile industries, who upheld nationalism as a result of their conflicts with the non-German speaking portions of the workforce, especially in the railway systems. In 1899, Stein was able to convene a workers' congress in Eger and promulgated a 25-point program.

Another convention was called in April 1902, under the title of "German-Political Workers' Association for Austria" (German: Deutschpolitischer Arbeiterverein fr sterreich), in Saaz. In Aussig, on 15 November 1903, they reorganized under the name of the "German Workers' Party in Austria" (German: Deutsche Arbeiterpartei in sterreich). At further party congresses, Hans Knirsch proposed to call themselves the "Nationalsozialistische" (National-Socialist) or "Deutsch-soziale" (German-social) Workers' Party. The proposal was blocked by the Bohemian groups, who did not want to copy the name of the Czech National Social Party. An early member of this group is Ferdinand Burschowsky, a printer from Hohenstadt (Moravia), who was active in writing and publishing.

At a party congress in Vienna in May 1918, the DAP changed its name to the Deutsche Nationalsozialistische Arbeiterpartei (DNSAP) and produced a National Socialist Program, which is thought to have influenced the later German Nazi manifesto.[citation needed]

The Austrian DNSAP split into two factions in 1923, the Deutschsozialen Verein (German-Social Association) led by Dr. Walter Riehl, and the Schulz-Gruppe. After 1930, most former DNSAP members became supporters of the German NSDAP led by Austrian-born Adolf Hitler, and were one of the chief elements leading the pro-Nazi coup in 1938 that brought about the Anschluss of Austria with Germany.

Leaders of the party, who were dubbed Landesleiter due to the recognition of Hitler as overall Fhrer, included Alfred Proksch (193133), Hermann Neubacher (1935) and Josef Leopold (193638), although real power frequently lay with Theodor Habicht, a German sent by Hitler to oversee Nazi activity in Austria.

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Nazism | Definition, Leaders, Ideology, & History | Britannica

Nazism had peculiarly German roots. It can be partly traced to the Prussian tradition as developed under Frederick William I (16881740), Frederick the Great (171268), and Otto von Bismarck (181598), which regarded the militant spirit and the discipline of the Prussian army as the model for all individual and civic life. To it was added the tradition of political romanticism, with its sharp hostility to rationalism and to the principles underlying the French Revolution, its emphasis on instinct and the past, and its proclamation of the rights of Friedrich Nietzsches exceptional individual (the bermensch [Superman]) over all universal law and rules. These two traditions were later reinforced by the 19th-century adoration of science and of the laws of nature, which seemed to operate independently of all concepts of good and evil. Further reinforcements came from such 19th-century intellectual figures as the comte de Gobineau (181682), Richard Wagner (181383), and Houston Stewart Chamberlain (18551927), all of whom greatly influenced early Nazism with their claims of the racial and cultural superiority of the Nordic (Germanic) peoples over all other Europeans and all other races.

Hitlers intellectual viewpoint was influenced during his youth not only by these currents in the German tradition but also by specific Austrian movements that professed various political sentiments, notably those of pan-Germanic expansionism and anti-Semitism. Hitlers ferocious nationalism, his contempt of Slavs, and his hatred of Jews can largely be explained by his bitter experiences as an unsuccessful artist living a threadbare existence on the streets of Vienna, the capital of the multiethnic Austro-Hungarian Empire.

Adolf Hitler (third from right) participating in a Nazi parade in Munich, c. 1930s.

This intellectual preparation would probably not have been sufficient for the growth of Nazism in Germany but for that countrys defeat in World War I. The defeat and the resulting disillusionment, pauperization, and frustrationparticularly among the lower middle classespaved the way for the success of the propaganda of Hitler and the Nazis. The Treaty of Versailles (1919), the formal settlement of World War I drafted without German participation, alienated many Germans with its imposition of harsh monetary and territorial reparations. The significant resentment expressed toward the peace treaty gave Hitler a starting point. Because German representatives (branded the November criminals by Nazis) agreed to cease hostilities and did not unconditionally surrender in the armistice of November 11, 1918, there was a widespread feelingparticularly in the militarythat Germanys defeat had been orchestrated by diplomats at the Versailles meetings. From the beginning, Hitlers propaganda of revenge for this traitorous act, through which the German people had been stabbed in the back, and his call for rearmament had strong appeal within military circles, which regarded the peace only as a temporary setback in Germanys expansionist program. The ruinous inflation of the German currency in 1923 wiped out the savings of many middle-class households and led to further public alienation and dissatisfaction.

Learn about the rise of Adolf Hitler, the Nazi Party, and the anti-Semitism they fomented in pre-WWII Germany

In 1933 Adolf Hitler's National Socialists were voted into power, and the campaign of terror began. From The Second World War: Prelude to Conflict (1963).

Hitler added to Pan-Germanic aspirations the almost mystical fanaticism of a faith in the mission of the German race and the fervour of a social revolutionary gospel. This gospel was most fully expressed in Hitlers personal testament Mein Kampf (192527; My Struggle), in which he outlined both his practical aims and his theories of race and propaganda.

Posing as a bulwark against communism, Hitler exploited the fears aroused in Germany and worldwide by the Bolshevik Revolution in Russia and the consolidation of communist power in the Soviet Union. Thus, he was able to secure the support of many conservative elements that misunderstood the totalitarian character of his movement.

Hitlers most important individual contribution to the theory and practice of Nazism was his deep understanding of mass psychology and mass propaganda. He stressed the fact that all propaganda must hold its intellectual level at the capacity of the least intelligent of those at whom it is directed and that its truthfulness is much less important than its success. According to Hitler:

It is part of a great leaders genius to make even widely separated adversaries appear as if they belonged to but one category, because among weakly and undecided characters the recognition of various enemies all too easily marks the beginning of doubt of ones own rightness.

Hitler found this common denominator in the Jewish people, whom he identified with both Bolshevism and a kind of cosmic evil. Jews were to be discriminated against not according to their religion but according to their race. Nazism declared Jewswhatever their educational and social achievementsto be forever fundamentally different from and inimical to Germans.

Nazism attempted to reconcile conservative, nationalist ideology with a socially radical doctrine. In so doing, it became a profoundly revolutionary movementalbeit a largely negative one. Rejecting rationalism, liberalism, democracy, the rule of law, human rights, and all movements of international cooperation and peace, it stressed instinct, the subordination of the individual to the state, and the necessity of blind and unswerving obedience to leaders appointed from above. It also emphasized the inequality of humans and races and the right of the strong to rule the weak; sought to purge or suppress competing political, religious, and social institutions; advanced an ethic of hardness and ferocity; and partly destroyed class distinctions by drawing into the movement misfits and failures from all social classes. Although socialism was traditionally an internationalist creed, the radical wing of Nazism knew that a mass base existed for policies that were simultaneously anticapitalist and nationalist. However, after Hitler secured power, this radical strain was eliminated.

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National Bolshevism – Wikipedia

Political ideology combining extreme nationalism and communism

National Bolshevism (Russian: -, romanized:Natsional-bol'shevizm, German: Nationalbolschewismus), whose supporters are known as National Bolsheviks (Russian: -, romanized:Natsional-bol'sheviki) or NazBols (Russian: , romanized:Natsboly),[1] is a radical political movement that combines ultranationalism (or alternatively fascism in some cases) and communism.[2][3]

Notable historical proponents of National Bolshevism in Germany included Ernst Niekisch (18891967), Heinrich Laufenberg (18721932), and Karl Otto Paetel (19061975). In Russia, Nikolay Ustryalov (18901937) and his followers, the Smenovekhovtsy, used the term.

Notable modern advocates of the movement include Aleksandr Dugin and Eduard Limonov, who led the unregistered and banned National Bolshevik Party (NBP) in the Russian Federation.[4]

National Bolshevism as a term was first used to describe a current in the Communist Party of Germany (KPD) and then the Communist Workers' Party of Germany (KAPD) which wanted to ally the insurgent communist movement with dissident nationalist groups in the German army who rejected the Treaty of Versailles.[5] They were led by Heinrich Laufenberg and Fritz Wolffheim and were based in Hamburg. Their expulsion from the KAPD was one of the conditions that Karl Radek explained was necessary if the KAPD was to be welcomed to the Third Congress of the Third International. However, the demand that they withdraw from the KAPD would probably have happened anyway. Radek had dismissed the pair as National Bolsheviks, the first recorded use of the term in a German context.[6]

Radek subsequently courted some of the radical nationalists he had met in prison to unite with the Bolsheviks in the name of National Bolshevism. He saw in a revival of National Bolshevism a way to "remove the capitalist isolation" of the Soviet Union.[3]

During the 1920s, a number of German intellectuals began a dialogue which created a synthesis between radical nationalism (typically referencing Prussianism) and Bolshevism as it existed in the Soviet Union. The main figure in this was Ernst Niekisch of the Old Social Democratic Party of Germany, who edited the Widerstand journal.[7]

A National Bolshevik tendency also existed with the German Youth Movement, led by Karl Otto Paetel. Paetel had been a supporter of the National Socialist German Workers' Party (NSDAP), but became disillusioned with them as he did not feel they were truly committed to revolutionary activity or socialist economics. His 1930-formed movement, the Group of Social Revolutionary Nationalists, sought to forge a third way between the NSDAP and the KPD, emphasising both nationalism and socialist economics.[8] He was especially active in a largely unsuccessful attempt to win over a section of the Hitler Youth to his cause.[9]

Although members of the NSDAP under Adolf Hitler did not take part in Niekisch's National Bolshevik project and usually presented Bolshevism in exclusively negative terms as a Jewish conspiracy, in the early 1930s there was a parallel tendency within the NSDAP which advocated similar views. This was represented by what has come to be known as Strasserism. A group led by Hermann Ehrhardt, Otto Strasser and Walther Stennes broke away in 1930 to found the Combat League of Revolutionary National Socialists, commonly known as the Black Front.[10]

After the Second World War, the Socialist Reich Party was established, which combined neo-Nazi ideology with a foreign policy critical of the United States and supportive of the Soviet Union, which funded the party.[11][12]

As the Russian Civil War dragged on, a number of prominent Whites switched to the Bolshevik side because they saw it as the only hope for restoring greatness to Russia. Amongst these was Professor Nikolai Ustrialov, initially an anti-communist, who came to believe that Bolshevism could be modified to serve nationalistic purposes. His followers, the Smenovekhovtsy (named after a series of articles he published in 1921) Smena vekh (Russian: change of milestones), came to regard themselves as National Bolsheviks, borrowing the term from Niekisch.[13]

Similar ideas were expressed by the Evraziitsi movement and writers such as D. S. Mirsky, and the pro-monarchist Mladorossi. Joseph Stalin's idea of socialism in one country was interpreted as a victory by the National Bolsheviks.[13] Vladimir Lenin, who did not use the term National Bolshevism, identified the Smenovekhovtsy as a tendency of the old Constitutional Democratic Party who saw Russian communism as just an evolution in the process of Russian aggrandisement. He further added that they were a class enemy and warned against communists believing them to be allies.[14]

Ustryalov and others sympathetic to the Smenovekhovtsy cause, such as Aleksey Nikolayevich Tolstoy and Ilya Ehrenburg, were eventually able to return to the Soviet Union and following the co-option of aspects of nationalism by Stalin and his ideologue Andrei Zhdanov enjoyed membership of the intellectual elite under the designation non-party Bolsheviks.[15][16] Similarly, B. D. Grekov's National Bolshevik school of historiography, a frequent target under Lenin, was officially recognised and even promoted under Stalin, albeit after accepting the main tenets of Stalinism.[17]It has been argued that National Bolshevism was the main impetus for the revival of patriotism as an official part of state ideology in the 1930s.[18][19] Although many of the original proponents of National Bolshevism such as Ustryalov and members of the Smenovekhovtsy were suppressed and executed during the Great Purge for national chauvinism, anti-Soviet agitation and other counter-revolutionary activities.[20][21]

The term National Bolshevism has sometimes been applied to Aleksandr Solzhenitsyn and his brand of anti-communism.[22] However, Geoffrey Hosking argues in his History of the Soviet Union that Solzhenitsyn cannot be labelled a National Bolshevik since he was thoroughly anti-Stalinist and wished a revival of Russian culture that would see a greater role for the Russian Orthodox Church, a withdrawal of Russia from its role overseas and a state of international isolationism.[22] Solzhenitsyn and his followers, known as vozrozhdentsy (revivalists), differed from the National Bolsheviks, who were not religious in tone (although not completely hostile to religion) and who felt that involvement overseas was important for the prestige and power of Russia.[22]

There was open hostility between Solzhenitsyn and Eduard Limonov, the head of Russia's unregistered National Bolshevik Party. Solzhenitsyn had described Limonov as "a little insect who writes pornography" and Limonov described Solzhenitsyn as a traitor to his homeland who contributed to the downfall of the Soviet Union. In The Oak and the Calf, Solzhenitsyn openly attacked the notions that the Russians were "the noblest in the world" and that "tsarism and Bolshevism [...] [were] equally irreproachable", defining this as the core of the National Bolshevism to which he was opposed.[23]

The current National Bolshevik Party (NBP) was founded in 1992 as the National Bolshevik Front, an amalgamation of six minor groups.[25] The party has always been led by Eduard Limonov. Limonov and Dugin sought to unite far-left and far-right radicals on the same platform.[26] With Dugin viewing national-bolsheviks as a point between communist and fascists, and forced to act in the peripheries of each group.[citation needed] The group's early policies and actions show some alignment and sympathy with radical nationalist groups, albeit while still holding to the tenets of a form of Marxism that Dugin defined as "Marx minus Feuerbach, i. e. minus evolutionism and sometimes appearing inertial humanism.", but a split occurred in the 2000s which changed this to an extent. This led to the party moving further left in Russia's political spectrum, and led to members of the party denouncing Dugin and his group as fascists.[27] Dugin subsequently developed close ties to the Kremlin and served as an adviser to senior Russian official Sergey Naryshkin.[28][29]

Initially opposed to Vladimir Putin, Limonov at first somewhat liberalized the NBP and joined forces with leftist and liberal groups in Garry Kasparov's United Civil Front to fight Putin.[30] However, he later expressed more supportive views of Putin following the 2014 pro-Russian unrest in Ukraine.[31][32][33]

The Franco-Belgian Parti Communautaire National-Europen shares National Bolshevism's desire for the creation of a united Europe as well as many of the NBP's economic ideas. French political figure Christian Bouchet has also been influenced by the idea.[34]

In 1944, Indian nationalist leader Subhas Chandra Bose called for "a synthesis between National Socialism and communism" to take root in India.[35]

That same year, the new leadership of the Israeli paramilitary organization Lehi declared its support for National Bolshevism, a break from the group's fascist outlook under its previous leader Avraham Stern.[36]

Some have described the Serbian Radical Party, the Bulgarian Attack party, the Slovenian National Party and the Greater Romania Party as "National Bolshevik" for blending much of their respective countries' far-right rhetoric with traditional left-wing stances such as socialised economies, anti-imperialism and defense of historical communist rule.[citation needed] The Serbian Radical Party in particular has given support to leaders such as Muammar Gaddafi,[37] Saddam Hussein[38] and current Venezuelan President Nicolas Maduro.[39] The Greater Romania Party on the other hand was founded by Corneliu Vadim Tudor, described as the "Court Poet of Nicolae Ceauescu",[40] and has been seen as a continuation of the latter's ideology with a right-wing veneer.

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Netaji Believed Indian Nationalism Was All About The Highest Ideals Of Human Race – Outlook India

In The Indian Struggle, Subhas Chandra Bose had outlined the reasons why he foresaw that communism would never be adopted in India. Firstly, he wrote, communism had no sympathy with nationalism, whereas the freedom movement was a national movement. Lenins thesis on the relation between nationalism and communism had been sidestepped after the failure of the Chinese revolution. The second reason was Russias disinterest in sparking a world revolution, with its focus on internal affairs and a decline in its prestige due to its pacts with capitalist countries and the joining of the League of Nations. Thirdly, the anti-religious and atheistic character of Russian communism would not fit into the Indian environment, where a national awakening is in most cases heralded by a religious reformation and a cultural renaissance. The fifth reason, he felt, was that although communist theory had made certain remarkable contributions in the domain of economics such as state planning, it was weak in other aspects Bose. Later, however, he retracted his argument about the conflict between nationalism and communism at an interview with Rajani Palme Dutt in January 1938, where he said:

I should point out also that Communism, as it appeared to be demonstrated by many of those who were supposed to stand for it in India, seemed to me anti-national, and this impression was further strengthened in view of the hostile attitude which several among them exhibited towards the Indian National Congress. It is clear, however, that the position today has fundamentally altered. I should add that I have always understood and am quite satisfied that Communism, as it has been expressed in the writings of Marx and Lenin and in the official statements of policy of the Communist International, gives full support to the struggle for national independence and recognises this as an integral part of its world outlook.

His later utterances, however, indicate that this statement was more a tactical retreat than a change in belief.

Subhas had urged Jawaharlal to preside over the All-Bengal Students Conference in Calcutta, held on 22 September 1928. Like Subhas, Jawaharlal too sang paeans to the youth, and had similar things to say about empowering different disadvantaged groups in the society, but the thrust of his message was very different. His was a message of socialism in contrast to nationalism. I have placed before you the ideals of internationalism and socialism as the only ideals worthy of the fine temper of the youth, he told them. As far as his attitude to communism was concerned, he said that:

. . . though personally I do not agree with many of the methods of the communists and I am by no means sure to what extent communism can suit the present conditions in India, I do believe communism as an ideal of society. For essentially it is socialism, and socialism I think is the only way if the world is to escape disaster.

Speaking at the same conference, Subhas outlined where he differed with Jawaharlal in the sphere of ideas. He too believed in internationalism, but not in the form which obliterated distinctive characteristics of different nations. The expression of nationalism by Chittaranjan, a humanist, poet, essayist, thinker and a politician fiercely proud about his Bengali heritage rolled into one, was in stark contrast to the critique of nationalism by Tagore. In their quarrel, Subhas was completely on his political mentors side. From Mandalay Jail, he had criticized the shallow internationalism in life and literature of Tagore and his school which did not realize the fundamental truth in nationalism. He would touch upon the topic now and then, but his speeches and writings lacked the lyrical exuberance of his guru. While speaking at the Maharashtra Provincial Conference, Subhas responded to the charge of nationalism being narrow, selfish and aggressive from the perspective of cultural internationalism: his response was more in the nature of a political project that he envisioned rather than at an abstract, conceptual level. He pointed out that Indian nationalism, far from being any of these, was inspired by the highest ideals of the human race, viz., Satyam (the true), Shivam (the good), Sundaram (the beautiful):

Nationalism in India has instilled into us truthfulness, honesty, manliness and the spirit of service and sacrifice. What is more, it has roused the creative faculties which for centuries had been lying dormant in our people and, as a result, we are experiences a renaissance in the domain of Indian art.

In fact, he would return to the philosophical aspects of the independence movement repeatedly in his speeches around this time. Organizing the movement was the immediate problem, but equally important was to impart a character to it. Subhas was not yet advocating a particular form of political and social organization for independent India. Rather, he focused on defining the components which would make up the whole, and wished to lay the ground for post-independence reconstruction. He was preoccupied with questions such as: What was the most desirable political system for Indias development? What were the roles of the students and the youth? How should India balance nationalism and internationalism?

The ideal of the youth was to break the shackles of all oppression, injustices, and malpractices to create a new nation, he told a youth conference at Pabna. Creation of a new order had to follow the destruction of status quo. Subhas invoked Krishnas stern castigation of Arjun in the battlefield of Kurukshetra Klaivyam masma gamah Partha as the message which contained the essence of immortal youth. The youth movement, just like the national movement, was not merely a political movement. It had to weave together varying strands of art, literature, philosophy, science, commerce, and sports for the development of national life.

He refused to stand behind any particular ism at this point. No ism (he referred to anarchism, socialism, communism, Bolshevism, syndicalism, republicanism, constitutional monarchy, and fascism) was adequate to lift humanity out of misery unless individual characters were strengthened. Indians, he said, had every quality except tenacity of purposewhat was needed, therefore, was the ability to sacrifice everything for the sake of an idea. As Vivekananda had pointed out, the basis of nation formation as well as of establishing an effective ism was to create good human beings first. Whatever ism India chose for itself, it had to be moulded in accordance with its traditions and had to answer well to the requirements of present conditions. It was equally important to pay attention to the nationalist aspect of the movement as to the internationalist aspect. The latter was required to be the foundation of lasting global peace based on common understanding, development and exchange of knowledge, and the emphasis on the latter was critical to be able to create a nation based on new ideals. The responsibility for this regeneration was on the shoulders of the youth.

The national awakening would not take place unless the foundations of Indian society were shaken up. For most people, as he pointed out, social oppression was a greater reality than political oppression. The never-changing aim was complete freedom social, economic and political. Most people could relate more immediately to social oppression than to state-led oppression, and it was futile to expect the oppressed sections to join in the political movement. He said that the hypocrisy that is going on in the name of society, religion and state must be crushed ruthlessly. Therefore, Privileges based on birth, caste or creed should go, and equal opportunities should be thrown open to all irrespective of caste, creed or religion.

If it was important to break out of the restrictive traditions of the past, it was equally important to remain connected with the achievements of the past and be proud of ones heritage. The politics of nation-making did not take away anything from his consciousness of being a Bengali. In his presidential address the 1929 Bengal Provincial Conference at Rangpur in North Bengal (now in Bangladesh), Subhas took his audience through the history of the province, stressing its tradition of rebelliousness, the unique identity the region maintained in ancient times, the vivacity of the Bengalis leading to experimentations with social and political forms and their achievements in spreading ideas in other countries, the syncretic culture during the Muslim rule, the reform movements, and lastly the emergence of the nationalist movement. Aware of the increasing influence of Marxist thought, especially amongst the revolutionary groups, he reiterated his conception of Indias traditional forms of socialism. Vivekananda and Deshbandhu were the icons of his variant of socialism, who struggled throughout their lives to uplift the downtrodden. Again, he voiced his opposition to accepting any ism as a package. Every ism had elements of truth what was good in socialism should be accepted; but accepting tenets of socialism did not mean that the discipline, organisation and obedience of fascism were to be disregarded. He referred to how the Soviet Union had to implement the New Economic Policy in contravention to the orthodox communist philosophy to suit its unique needs. He argued that if an ism is imposed on a country by disregarding its history and its present conditions, it would either lead to a revolution or give rise to a contrary ideology like fascism. Most important, however, was the development of personality. No ism could succeed without good human beings.

He was only too aware of the barriers to building up that mass movement in the form of caste and religious divisions, the position of women in the society and the tentative links between the Congress and the industrial labour and peasants. Throughout this period, these themes kept resonating in his speeches as the basis of attaining social, economic and political freedom.

(Excerpt from Bose: The Untold Story of An Inconvenient Nationalist by Chandrachur Ghose, with permission from Penguin Random House)

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What it’s like to be targeted by anti-vaxxers – The New Statesman

This week I met a ghost. Not some movie caricature of a risen spirit but something far more ghoulish and real: the resurrected corpse of tyranny, fundamentalism, and sheer hatred.

Im a columnist for the Toronto Star, one of Canadas largest newspapers and very much the voice of the countrys liberal conscience. On Monday 17 January I wrote about the anti-vaccination movement. Not a deep analysis or a thorough exploration, but a personal account the story of my great-aunt from Eastern Europe, who would care for me when I was a child, and of the death camp numbers tattooed onto her arm, and the slaughter by the Nazis of many of her, and my, family.

How, I asked, could the opponents of the vital and compassionate Covid vaccination programme compare themselves to people ripped from their homes, tortured, humiliated, and murdered? How dare they parade the yellow star at their demonstrations and on their social media platforms, try to hijack the Holocaust, and compare their privileged and self-imposed experience to genocide? It was the world turned upside down, the denial of reality, the desecration of memory and loss.

Thats when the ghost came out to play. The column was intensely personal, difficult to write, and there were numerous people who championed it. But not the anti-vaxx militants, whom I now realise move in dark and fierce regiments.

The tweets and emails began within moments of the column being published. Im an old hand at this sort of thing and have been attacked by all sorts of nutters and nasties over the years, some of them highly motivated and belligerent. But never, ever anything like this. In 48 hours there had been thousands of attacks. Many of them were, of course, bot accounts, meant to destabilise and polarise, and theyre obvious by their style and appearance. But most appeared to be from real people.

As Im a priest, dozens of them alleged that I was a child abuser, a paedophile. They varied in their explicit and gross nature. Then there were the hundreds convinced that I was a Satanist, a devil-worshipper. One had me as a Son of Satin. Hey, its lovely material.

They posted pictures of my body with a twisted, grotesque face, they told downright lies about my life and work, superimposed my face on pictures of Hitler and Goebbels, and libeled members of my family. And they threatened me, some demanding that I be hanged. (Well, they said hung but what does one expect?) Friendly voices recommended contacting the police, but while they meant well they didnt appreciate how pointless this usually is. Unless there is evidence of who is behind such threats, the cops are largely powerless. I made sure the doors were locked, paid more attention to my surroundings, comforted myself that it was all bombast.

They found my website of course, and obtained my personal email. None of this is hidden, and as a cleric who deals with pastoral emergencies and hospital visits my details are out there. I wish I could say it didnt matter and that I didnt care but that would be untrue. Putting on a brave face isnt the same as having no feelings. Eventually it will fade and they will move on to another target but the sobering aspect, the thought that rests permanently in the mind, is that in the end theres actually not very much one can do about it.

So who are they, these acidic warriors in the fight against vaccinations, masks, and lockdowns? Various people, naturally, but broadly speaking hyper libertarians, religious mostly Christian fundamentalists, conspiracy theorists, and the weak and gullible. Its a dangerous brew, as were seeing increasingly in acts of violence and civil disobedience. In North America the situation is more acute, more concerning. The mob that stormed the Capitol Building in Washington, DC on 6 January 2021 was composed of precisely the sort of people Ive just listed. Since then theyve become more radicalised, feel more marginalised, and are certainly more organised.

This jarring disconnect, this politicised mass psychosis, may have its origins in the US but has been replicated internationally; not always as large, and thank God not as well-armed, but its there, like a toxin of hysteria and ignorance pumped into the bloodstream of the body politic. Or even ironically a potentially deadly virus. Most of us find it all somewhat difficult to comprehend, because the belief system behind it is downright deranged, and can so easily be proven wrong. But the same can be said of fascism and racism. The same could be said, was said, but sometimes and with ghastly consequences still failed to win the day.

If the gutter press could influence millions in the 1930s, social media and Fox News can do so just as efficiently almost a century later. As many others have said before, the lie told convincingly and frequently becomes the truth, the fiction becomes the fact. I wasnt attacked by people who thought they were bad, I was attacked by people who knew I was. Unless we grasp this we can never understand the problem.

Contrary to what several thousand people told me in the past few days, the Covid vaccine isnt designed to control world population, sterilise us, or make us instantly identifiable to the new world order. Its not the sign of the beast, not connected to some eschatological fantasy, and President Joe Biden isnt dead and being played in public by a Moscow-trained actor. And no, Im not the anti-Christ.

It all seems so bizarre, so ridiculous, even so irrelevant. Until it isnt.

My bubba, about whom I wrote that column, thought that National Socialism was a circus of inadequate malcontents and that civilized, noble Germany would come to its senses. So we should be moderate in our response but be informed and aware. What is a campaign against vaccinations today could wear a different uniform tomorrow. I wish I could say otherwise, but the people and the beliefs Ive encountered this week arent simply going to disappear.

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What it's like to be targeted by anti-vaxxers - The New Statesman

Oppose the dangerous reopening of universities! Students and youth must take up a fight against the pandemic! – WSWS

This statement will be distributed by the International Youth and Students for Social Equality (IYSSE) club members to students and young people around Australia as universities resume for the 2022 academic year.

The start of 2022 has brought young people around Australia face to face with the cruel reality of the ruling elites criminal policies amid the COVID-19 pandemic.

Australia was once hailed around the world as a success story for keeping case numbers and deaths low relative to countries like the US, Brazil, and those in western Europe. The country is now experiencing 100,000 daily infections spurred on by the highly transmissible Omicron variant. Fatality records are being broken multiple times a week, with 398 deaths in the last seven days alone.

The vertical spike in Australian COVID infections is a result of the murderous live with the virus program promoted by governments and the corporate media around the world. Australian federal and state governmentsLiberal-National Coalition and Labor alikehave proceeded amid the surge in cases with the pro-business reopening of business, schools, and universities in the interests of the profits of the financial oligarchy, no matter the impact on the lives of ordinary people.

Young people and students must take up a fight against this homicidal profit-driven drive!

Youth are being placed on the frontlines of the governments ghoulish experiment on the population. As part of the campaign to reopen, universities and schools plan to return to in-person teaching. Cramming students around the country into crowded classrooms and lecture theatres will cause an uncontrolled spread of the deadly virus, resulting in further mass death and suffering.

The death earlier this month of fit, young, healthy, double-vaccinated James Kondilios in Sydney underscores that young people are not safe from the virus. It cuts across this government-media lieused to justify the profit-driven campaign to live with the virusthat youth are invulnerable to the effects of COVID-19. Child hospitalisations amid the Omicron surge are at record levels in almost every country. Unmentioned in the mainstream media as well are the effects of Long COVID, which are not yet fully understood. What is known, however, is that suffering can persist for many months after initial infection.

Despite the staggering case numbers, and record hospitalisations and deaths due to COVID-19, university campuses around the country are marching in lockstep with the calls by big business to resume face-to-face teaching.

Monash University in Melbourne announced that it will reopen its campus on January 27, while the University of Melbourne will have a blended online and on-campus model for onshore students. Sydneys University of New South Wales is also planning a return to campus for the first term of 2022.

University workers at Melbournes Victoria Universitywhich has a large working-class and immigrant student populationwere advised before Christmas that summer courses would resume in person despite COVID-19 case numbers spiraling out of control.

This reopening has gone ahead with the full support of the pro-business trade unions. National Tertiary Education Union (NTEU) Assistant National Secretary Gabe Gooding penned an article in the unions magazine, Advocate, on November 8, 2021, entitled Reflections on leaving lockdown and the road ahead. While the current surge due to the spread of Omicron had not yet begun, daily case numbers nationally at the time were over 1,000. In the article, Gooding wrote: This period as we transition out of lockdowns is the point where we must seize the moment and ensure that we dont transition back, but instead transition forward.

The NTEU has not written a word since on the exponential rise of cases around the country, let alone opposed the drive to reopen universities. The National Union of Students has similarly remained silent on the reopening of university campuses amid mass infections.

Australian children are being herded into unsafe classrooms. No longer is there any pretence that it is safe for children or their teachers or in the interests of their education. It is to force their parents back to work to produce profits for the financial elite. This is occurring under conditions in which many under 12-years-old remain unvaccinated. Australian Prime Minister Scott Morrison insisted emphatically last week: Our objective is go back, stay back, day one term one.

Young workers employed in hospitality, retail, warehousing, delivery services, and other industries are on the frontline of the dangerous spread of the virus. They often have to work multiple jobs and living in cramped accommodation in order to pay for rent, food, bills, education, and other living expenses. In these sectors, youth are being pressured by bosses and their financial situation to return to work despite the dangers amid mass transmission.

In recent interviews published on the World Socialist Web Site, young workers exposed their unsafe work conditions and the indifference of government and business policies to the dangers that workers confront amid the live with the virus campaign.

But a shift is underway. Young people, students, and workers are not going to sit back and allow this mass death and misery to continue. Internationally, there is a growing movement of the working class against the homicidal big business policy of herd immunity and mass infection.

Teachers aroundtheworldarespeakingout in opposition to the murderous plans to return to face-to-face teaching. In France, an estimated 75 percent of teachers participated in a national strike. In Chicago, 25,000 teachers took part in a powerful protest against the school reopening policies pursued by the Democratic Party on behalf of big business. There is also growing opposition among high school students in Australia to school reopenings while infections continue to rise. Educators and their supporters organised outside the politically bankrupt trade union apparatus in rank-and-file committees in Sri Lanka, Germany, Australia, the UK, Canada and elsewhere have discussed the need to stop reckless reopening of schools during the pandemic.

Internationally, there have been similar developments among autoworkers, postal workers, transport workers, and in other industries. It is to this social forcethe international working classthat young people and students must turn in order to end the pandemic and save lives.

The COVID-19 pandemic is a symptom of a much more terminal diseasethe decaying capitalist system. The pandemic crisis was predicted by modern science, and scientific knowledge also shows that it was entirely preventable and can still be ended. The suppressionofthevirusinChina since the initial outbreak shows that scientifically-determined public health measures taken togetherstrict lockdowns, mask mandates, proper quarantining facilities, mass vaccination programs, and adequate contact tracingcan eliminate COVID-19. But this cannot be maintained in a single country. The eradication of COVID-19 requires an internationally-coordinated program of elimination.

However, this scientific program has been rejected by the tiny, wealthy minority as the closure of businesses would cut across their ability to make profits through the exploitation of the working class. The financial oligarchy in every country have continued to engorge themselves while workers, their families, small businesses and whole societies made to suffer. The drive to reopen the economy, including the resumption of in-person teaching in schools and universities, is an exercise in social murder at the expense of capitalist profits.

The World Socialist Web Site, published by the world Trotskyist movement of which the IYSSE is the global youth and student movement, has characterised the pandemic as a trigger event in world history, akin to the First World War, which will have a profound impact on social consciousness. Millions of workers and youth internationally are gaining an education outside of schools and universities. That lesson is in the abject criminality of the capitalist system which places profits over lives.

The International Youth and Students for Social Equality insists that the pandemic raises centrally the question of political program. Capitalism has not only produced the COVID-19 pandemic crisis but is also the cause of all the dire social problems confronting humanity: the drive to another catastrophic world war, environmental destruction, growing social inequality, and the threat of fascism.

The latest Oxfam briefing on inequality summed up the state of class relations in the world today. A new billionaire has been created every 26 hours, since the beginning of the pandemic in early 2020 it stated. The report continued: The worlds 10 richest men have doubled their fortunes, while over 160 million people are projected to have been pushed into poverty. Meanwhile, an estimated 17 million people have died from COVID-19a scale of loss not seen since the Second World War.

If the capitalist system cannot prevent misery and mass death for the worlds population, then it must be overthrown and replaced with a socialist system to address the pressing needs of humankind.

As the only genuinely socialist revolutionary club on campus, the IYSSE will fight to mobilise youth and students against the criminal response of capitalism to the pandemic, and for an international revolutionary movement of the working class for socialism. We urge you to take your place in this struggle today by joining the IYSSE club or by forming one at your school or university. Contact the IYSSE to find out more.

Join the fight to defend public education! No unsafe return to schools!

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Oppose the dangerous reopening of universities! Students and youth must take up a fight against the pandemic! - WSWS

Marti’s thoughts applied to daily life keeps him alive in Cuba – Prensa Latina

Every day we must remember the heroes and martyrs, our founders, referents and paradigms, Palacios specified when making the conclusions of the central act for the 33rd anniversary of the movement, held in the Principal Theater, one of the architectural jewels of the city .

Thanks to Sancti Spritus for what it has done all this time, which is why it is the venue for this act, he said, and recognized that being a Martian is feeling his work, loving it and applying it.

He conveyed congratulations on behalf of the National Directorate of the MJM, the National Bureau of the Union of Young Communists (UJC) and the Office of the Mart Program, for being this province the most outstanding in the work of the aforementioned movement for two consecutive years.

He highlighted the importance of showing a true character, which according to his own words goes through ethics, the vocation of justice and being worthy.

That is what Mart calls us to do, he specified, indicating that the 169th anniversary of the birth of the Apostle of independence, the most universal of all Cubans, will be celebrated on the 28th.

He recalled the close friendship that united Mart and the Hero of the three Cuban wars of independence against Spanish colonialism, Serafn Snchez born in Sancti Spiritus.

Cuba must continue to be a headlight, because we are committed to socialism, social justice and equality, with everyone and for the good of all, as Mart expressed, he argued.

The Provincial Council of said movement, with the approval of the UJC Bureau in the territory, awarded the Abdala prize -a dramatic poem written by Mart- to outstanding educational and cultural institutions, and to Arley Rodrguez, a doctor at the Gaviota Complex.

Other institutions were recognized, such as the branch of the Jos Mart Cultural Society, and La Colmena Espirituana Dueos de la Felicidad, as well as the venue for the national act received by Orlando Ernesto Prez, president of the MJM in the province.

Also attending the national event were Deivy Prez, member of the Central Committee of the Party and first secretary in the territory, Teresita Romero, governor of Sancti Spritus, and Alicia Alonso, deputy minister of Higher Education.

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Marti's thoughts applied to daily life keeps him alive in Cuba - Prensa Latina

NH teachers react to proposed bill adding to Cold War-era ‘Teachers’ Loyalty’ law – WMUR Manchester

Republican lawmakers introduced additions to a Cold War-era statute that bans educators from advocating for communism in schools to the House committee on Thursday. The statute dates back to 1949.Rep. Alicia Lekas, R-Hudson was looking to add Marxism and socialism to the ban, as well as any idea that the United States was founded on racism.Lekas said the intent of House Bill 1255 is to ensure teachers are educating and not indoctrinating."When I only accept an answer that says that my political beliefs are right and your beliefs are wrong, thats indoctrination, Lekas said.The intention here is to make certain that in our schools our teachers are doing what has happened for a long time teaching how to think not what to think, said Rep. Erica Layon, R-Derry.People opposed to the bill say teachers will be silenced and scared to cover certain topics in the classroom for fear of punishment."Critical thinking isnt being allowed because theyre afraid theyre going to be reported for something, National Education Association New Hampshire President Megan Tuttle said."The idea that I show up to work every day for low wages in unsafe conditions and Im not loyal I would love to know what your definition of loyalty is if its not that, history teacher Jennifer Given said. The sponsor of the bill said she did not have time to properly draft the bill and she is currently working on an amendment.

Republican lawmakers introduced additions to a Cold War-era statute that bans educators from advocating for communism in schools to the House committee on Thursday.

The statute dates back to 1949.

Rep. Alicia Lekas, R-Hudson was looking to add Marxism and socialism to the ban, as well as any idea that the United States was founded on racism.

Lekas said the intent of House Bill 1255 is to ensure teachers are educating and not indoctrinating.

"When I only accept an answer that says that my political beliefs are right and your beliefs are wrong, thats indoctrination, Lekas said.

The intention here is to make certain that in our schools our teachers are doing what has happened for a long time teaching how to think not what to think, said Rep. Erica Layon, R-Derry.

People opposed to the bill say teachers will be silenced and scared to cover certain topics in the classroom for fear of punishment.

"Critical thinking isnt being allowed because theyre afraid theyre going to be reported for something, National Education Association New Hampshire President Megan Tuttle said.

"The idea that I show up to work every day for low wages in unsafe conditions and Im not loyal I would love to know what your definition of loyalty is if its not that, history teacher Jennifer Given said.

The sponsor of the bill said she did not have time to properly draft the bill and she is currently working on an amendment.

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NH teachers react to proposed bill adding to Cold War-era 'Teachers' Loyalty' law - WMUR Manchester

Jury hears opening statements in federal trial of three former cops involved in the murder of George Floyd – WSWS

Opening statements were heard on Monday in the federal trial of three former Minneapolis Police Department (MPD) officers charged with deprivation of civil rights for refusing to stop Derek Chauvin from killing George Floyd by kneeling on his neck on Memorial Day 2020.

The trial of J. Alexander Kueng, Thomas Lane, and Tou Thao is being presided over by Judge Paul Magnuson of the U.S. District Court in St. Paul, Minnesota.

The three men were charged, along with Chauvin, in an indictment by a federal grand jury in Minnesota nearly one year after the killing of Floyd. The charges of deprivation of civil rights were applied in three counts: the first against Chauvin for keeping his knee on George Floyd after he became unresponsive, the second against Thao and Kueng for willingly failing to intervene to stop Chauvin from using unreasonable force, and the third against all four of the former officers for willfully failing to provide aid to Floyd and acting with deliberate indifference when he was in distress and dying.

Chauvinwho was convicted of murdering Floyd in a state trial on April 10 and is now serving a 22.5-year prison sentencepleaded guilty on December 15 to the federal civil right charges against him. He is expected to receive an additional 2.5 years of prison time for the federal offense.

A jury of five men, seven womenwith an even number of white and non-white jurorsand six alternates was selected from across the state of Minnesota last Thursday. Judge Magnuson repeatedly stressed that the conviction of Chauvin on state murder charges and his guilty plea on federal charges must not influence the proceedings against the trial of the other three. Kueng, Lane and Thao also face a state criminal trial of their own that is scheduled to begin on June 13.

Assistant U.S. Attorney Samantha Trepel opened the prosecutions case saying that the three officers did not lift a finger to help George Floyd even though he said 25 times that he could not breathe. Speaking to the defendants directly, she said that Floyd was in your custody, in your care and then read from the MPDs policy about how people are to be treated once taken into police custody.

Using still images from a city surveillance camera, the prosecutor described the actions of each of the defendants as they made the conscious choice over and over again not to act to protect a man they had in handcuffs and pinned to the pavement. Protecting those in custody is not just a moral responsibility, its what the law requires under the U.S. Constitution, Trepel said.

Trepel singled out the actions of Thao, 36, who was a veteran officer and Chauvins partner. She showed in a bystander video how Thao was trying to keep at bay the angry crowd that was witnessing the murder and demanding that Chauvin get off George Floyds neck. Instead of trying to stop Chauvin from killing Floyd, she explained, Thao cynically told the witnesses, This is why you dont do drugs, kids.

The prosecutor acknowledged that both Kueng and Lane were rookie officers but said this was no excuse for their actions. She said even though it can be awkward and uncomfortable to criticize a fellow officer, the two had received extensive training for 1-1/2 years that use of force and the duty to intervene should have been fresh in their minds. Among the training that Trepel mentioned was the obligation to turn a subject on his/her side when they are having trouble breathing.

As to the third charge, the prosecutor said that the officers were trained in cardiopulmonary resuscitation but took no action to help save George Floyds life as he was rendered unconscious and lost a pulse. Here on May 25, 2020, for second after second, minute after minute, these three CPR-trained defendants stood or knelt next to Officer Chauvin as he slowly killed George Floyd right in front of them, she said.

In conclusion the Assistant US Attorney told the jury, We will ask you to hold these men accountable for choosing to do nothing and watch a man die.

Following the prosecutions opening statement, the attorney for Kueng, Thomas Plunkett, made a motion for a mistrial on the grounds that Trepels presentation to the jury was more argumentative than based on the evidence the prosecutors were planning to use during the trial. Judge Magnuson denied the motion.

In his opening statement of defense, Robert Paule, the attorney for Thao, attempted to blame George Floyd for his own death by saying it was a tragedy but a tragedy is not a crime. Paule told the jury to look beyond the video and see that Floyd was acting erratically and appeared to be on drugs when the officers attempted to arrest him.

According to a report in the New York Times, the defense appeared to be outlining a defense built on several pillars: That by initially resisting arrest, Mr. Floyds actions justified the aggressive police response; that Mr. Lane and Mr. Kueng were rookies, and that Mr. Chauvin was a training officer; and that Mr. Thao was busy keeping bystanders at bay, acting in the words of Mr. Paule as a human traffic cone.

Plunkett also suggested that he would try to blame the MPD for insufficient training of the rookie Kueng, and Earl Gray, attorney for Lane, was also pointing a finger at the city police department for keeping Chauvin on the force when he had a history of abuse and misconduct. Gray said that Lane had been told by superior officers that Chauvin was an excellent field training officer. Attorney Gray also said that his client intended to testify in his own defense, the only one of the three to do so, and said that Lane had two times tried to get Chauvin to roll George Floyd onto his side.

Legal experts have said that obtaining a conviction in the federal trial of Kueng, Lane and Thao is difficult because the prosecution must prove that the defendants are guilty of willingly violating George Floyds constitutional rights by not acting in his in defense rather than doing something directly to him.

The behavior of the then-Minneapolis officers is hardly unknown to working class and poor people in the US or other countries around the world. The actions of Kueng, Lane and Thao is part of an unstated law enforcement code of conduct where extreme brutality is followed by the victim being left unconscious or dying as officers mill around at the scene discussing how they will cover up their crime.

The only thing that is different in the death of George Floydand other similar cases recently, such as the shooting of Ahmaud Arbery in Glynn County, Georgiais the presence of the smartphone phone video shot by Darnella Frazier that was then viewed millions of times, triggering mass protests against police violence which swept across the US and internationally during the summer of 2020.

Additionally, the case of the three officers who cooperated with Derek Chauvin in killing George Floyd shoots a hole in the racialist presentation of police violence as purely a matter of blacks being killed by white police officers. The fact that Kueng is black, Lane is white, and Thao is Asian American makes clear that police violence is rooted in class society and capitalism, and the only way to stop it is by unifying the working class across all national, ethnic, racial and language barriers in the struggle for socialism.

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Jury hears opening statements in federal trial of three former cops involved in the murder of George Floyd - WSWS

Amidst Political Turmoil and Global Hardship, What Path Remains for Conservative Americans? – PR Web

This book details the result of the 2020 elections, the impact of the pandemic, the implosion of the leadership class, and the new coalition being built on the right.

MARION, Iowa (PRWEB) January 24, 2022

Author Tom Donelson offers his solution to pulling America out of the dark in America At The Abyss: Will America Survive? ($16.99, paperback, 9781662836725; $26.99, hard cover, 9781662836732; $7.99, e-book, 9781662836749).

Donelson sees a dark future for America if it continues its current trajectory, but he also believes there is hope. In this book, he explores solutions such as a new GOP coalition of rural Americans, and combining Trump populism with classical conservatism.

This book details the result of the 2020 elections, the impact of the pandemic, the implosion of the leadership class, and the new coalition being built on the right, said Donelson.

Tom Donelson has authored eight books including his most recent book, the Rise of National Populism and Democratic Socialism. Presently Mr. Donelson is the chief political strategist of Americas PAC and research associate and project director of Americas Majority Foundation. He has contributed for the past four decades to various publications, websites and blogs.

###

Liberty Hill Publishing, a division of Salem Media Group, is a leader in the print-on-demand, self-publishing industry. America At The Abyss is available online through amazon.com and barnesandnoble.com.

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Amidst Political Turmoil and Global Hardship, What Path Remains for Conservative Americans? - PR Web

Conflict, consensus, crisis. Three minimum notes on the protests – OnCubaNews

Most of the opinions circulating about the July 11 protests in Cuba, particularly those that reject disorder and violence, as well as those that interpret and propose solutions to the conflict, must be right. Many reflect civic concern and commitment to issues that go beyond personal interest. Seen like this, they would be a sign of social glue, of citizen participation and consensus. At the same time, they are the mirror of a significant conflictiveness.

In this brief space, I will avoid discussing good or bad intentioned interpretations, experiences lived, read or heard, recommendations to the government, etc. I only propose to take a step back, to coldly examine some basic problems, among the many that lie ahead.

What do the protests mean?

If we were to ask mile Durkheim, one of the founders of Sociology, what is the nature of these protests, he could answer that it is a classic case of anomie. Anomie defines a situation where previously established norms and values disintegrate; a typical reaction of periods of drastic and rapid changes in the social, economic or political structures of society. Social groups that experience anomic reactions may feel disconnected, as if they do not belong to their society, and as if their society does not value their identity. Anomie can cause purposelessness, hopelessness, and encourage deviance and crime. I have intentionally underlined a few keywords in this classic definition, which is the ABCs of sociology.

In Cuba, we have been going through a transition process for more than two decades, characterized by profound changes in the social structures and in the economic life of the people, but also in the relations between civil society and political power. Among other changes, lets say, there is the very idea of socialism, which now incorporates conceptions different from those defended for half a century, as well as unprecedented policies. This transition has made visible a crisis of norms and values, widely debated in various public spaces and media. Likewise, the weakening of the sense of belonging has been pointed out; and the reproduction of marginality and its typical behaviors, within subordinate neighborhoods and social groups, but also the proliferation of crime in other social and institutional spaces, where corruption grows. As for despair, the art and literature disseminated on the island are a good mirror.

In other words, what is happening in Cuba is an anomie that should not catch us by surprise, because its factors and manifestations have not remained hidden or muzzled, as anyone can see without having to read social networks or anti-government newspapers. It has been there, in front of everyone, analyzed and discussed for too long, to ask ourselves now where the protests come from, as if they were thunder in a clear sky. Rather, one should ask why they have not happened before.

How is it that the Cuban opposition, on the island and in Miami, using the fashionable unconventional war manuals, and the CIA itself, have not managed to unleash something like this until now? And why precisely now? Durkheim would resort to another concept shared by social sciences and civil engineering: fatigue. After a year and a half of COVID-19 and six months of queues to buy basic products as Dr. Durn would say we are all more vulnerable.

What is happening to the new government?

I have pointed out before that the consensus has become more heterogeneous and contradictory in Cuba, that it has incorporated dissent, and that the Cuban government knows it. Before taking office as president, Ral recognized that the leadership of the founder of the Revolution, Fidel, was not inherited. Daz-Canel, who was already in the Political Bureau in Fidels time, was also able to know this; and in any case, he has experienced it firsthand since he took office in 2018. In fact, continuity has entailed different ways than the historical ones did before. Circumstances, which are the benchmark of politics, had already been imposed on them before they retired.

I underline what I say about a new government, because if it is postulated that this is the same Cuba as Fidel and Ral, witty literary metaphors can be constructed, but the countrys political and social process is difficult to understand. This government has sought to build its own consensus from the beginning, instead of resting on what some call the political capital of the Revolution. However, the yardstick to measure change is already another.

In effect, the new government has proposed unprecedented reforms since 1960, beginning with a new Constitution, which admits a mixed economy, with markets and the private sector, and which grants unprecedented autonomy to local powers. Its new style, learned by holding leadership positions in the provinces, emphasizes the interaction between the central and local level; and it has ministers under the age of 60 explaining problems and answering questions on television. Unlike previous periods, citizens can identify them by their names, judge them, praise them or openly mock them.

There has never been a moment like this before in terms of freedom to criticize the government, on social networks, but neither in public media, nor to access information from very diverse sources, including those of the opposition; nor is there greater freedom to enter and leave the country. Article 56 of the Constitution approved in 2019 establishes the right of association and public demonstration. In fact, a demonstration law was scheduled in the legislative calendar for October 2020 postponed, along with a dozen other bills due to the coronavirus. Regardless, the prevailing yardstick dictates that this government has done far less than it should. According to that rod, its glass would be almost empty.

As if that were not enough, after a year and a half focused on a formidable global human security crisis called a pandemic, without resources or protective alliances like those of yesteryear, this government has had to deal with the largest manifestations of discontent that occurred since 1959 Going down the streets of San Antonio de los Baos, President Daz-Canel must have remembered, like all of us who lived the summer of 1994, Fidel followed by a sea of people, going down San Lzaro street, to control that outbreak of anomie on the Malecn, without weapons or specialized forces to deal with riots. In a certain way, he did exactly the same thing as Fidel: to appear at the scene of the events, and summon the revolutionaries to take to the streets and face violence, by force, if necessary.

The same media, however, may produce different results in other circumstances. It took him a few hours to realize it. But his first slogan was carried out to the letter, not only by the police, but by the summoned organizations, first of all, the Party. Across the street, the opposition, as on November 27, 2020, capitalized on the discontent and increased the tensions. The classic escalation of violence studied by experts in conflict resolution1 was immediate.

A more complicated scenario could not be imagined to maintain the route outlined in the 8th Party Congress, just 90 days ago.

What violence and how?

In a model country for many in terms of stability, citizen respect and internal order, such as Japan, protests against police brutality against foreigners or racism are not uncommon. A group of foreign (that is, Korean) protesters can gather around them a cloud of police officers dressed as characters from Star Wars, with polycarbonate helmets and armor, armored shields and tonfas.

We are used to seeing images of violent demonstrations in other countries. Those who throw stones are part of the people, who rebel against injustice; those who shoot jets of water from riot control vehicles, tear gas, rubber bullets, or real ones, are the repressive forces. These global images do not discriminate between countries such as Chile, South Africa, Kyrgyzstan or the United States, with hundreds of wounded and dozens of deaths that are their balance.

The photos and videos that circulate in the media such as BBC Mundo above all suspicion of collusion with the Cuban regime reveal that neither the National Revolutionary Police (PNR), which here in Cuba is the only police institution, nor the special troops from the Ministry of the Interior (MININT) or the Revolutionary Armed Forces (FAR) deploy those resources. Surely the Institute of Police Sciences of the MININT teaches how to face violent scenarios. But no class or exercise is equivalent to dealing with 700 angry people marching down the street under the summer sun or doing it by force if necessary even if its instructions say to avoid injuring them or using lethal means.

This is not a technical or circumstantial detail. Among the images of the protests that went viral on social networks, a dozen protesters turned police cars upside down, and even other vehicles, jumped on them and destroyed them. Compared to any capital in Latin America, there are no forces that prevent these attacks on authority, and that repress them at that time. At the same time, some police officers and civilians, summoned to mobilize in the theater of the confrontation, incurred excesses.

Among the few data available to measure physical violence is the looting of stores, in freely convertible currency and in Cuban pesos. There were none in San Antonio de los Baos; nor in Havana until after the television appearance of President Daz-Canel (4:30 pm). Of the 28 assaults registered up to that time, 68% (19) occurred in Matanzas, the province most affected by the pandemic; almost all of them in Crdenas (13), where the combination of the drop in tourism in Varadero plus the quarantine has hit a relatively higher standard of living than in other places in the province. In that period, there was only significant looting (4) in Coln (Matanzas), and Gines (Mayabeque); and others scattered in Holgun, Bayamo, Gira (1). After the Presidents intervention, 13 stores were raided, including 4 in Havana.

The social polarization that this violence shows is inversely proportional to unity, that is, to the construction of consensus. In addition, it has a negative impact on the image of the country, which works in favor of the cornerstone of U.S. policy: isolation. Preventing the battle won at the UN from being lost in the streets of Crdenas or Paseo del Prado is also a national interest.

After having tried everything with Fidel and Ral, and 25 years after the end of the Cold War, Barack Obama and his government considered that this policy was ineffective, according to their national interest. However, although Joseph Biden, vice president of that government, supported normalization, things have changed for them. What if Daz-Canel, without the wisdom and experience of the Castros, were not able to deal, at this moment of vulnerability, with the Cuban crisis? They might reason that it is better not to lower the heat on the blockade right now, but to let it continue to simmer the island. As you would say in Cuban: whats the rush?

The protests are lessons for all who want to read them. They could teach some economists that the success of the reforms does not depend only on technically solving planning, the market, the socialist state enterprise or the private sector, but on tackling problems such as income redistribution, consumption stratification, the adjoining economically brilliant or dark spaces, the inequalities and territorial and local setbacks, the state of the productive forces called workers. They have also shown politicians that the problem of national unity is that of consensus, and that it is not solved only with summoning mobilizations of revolutionaries, but through sustained dialogue with all citizens. They have shown the Party apparatuses, once again, that the effectiveness of a public media system is not ideological, but political, and that it is measured by its credibility and capacity to convince (the unconvinced, of course). They have confirmed that law enforcement agencies can provide first aid to outbreaks of violence, but at the cost of other damage, and that it is not they who should deal with the social and political problems where dissent takes root. Finally, it has shown U.S. politicians that its alliances with this bellicose opposition reinforces the hard line on both sides, and damages the real exercise of freedom and human rights in Cuba.

The common denominator of these lessons is Cuban society, with its lights and shadows. Knowing how to decipher its present, without bipolar roadmaps, will decide what will come.

Note:

1 Violencia y solucin de conflictos, in Temas magazine # 53, January-March, 2008.

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Conflict, consensus, crisis. Three minimum notes on the protests - OnCubaNews

Who will win the battle for ‘based’? – Spectator.co.uk

Earlier this week, a pair of right-libertarian journalists announced the launch of their new site, BASEDPolitics. All hell promptly broke loose on right-wing Twitter.In the first editorial for their new site, co-founders Brad Polumbo and Hannah Cox define 'based' as 'upfront, on point, or rooted in true principles.' That fits pretty well with my understanding of the term, but it leaves something out.

That 'something' accounts for the pushback they received from the post-liberal, national conservative crowd. According to them, libertarians like Polumbo and Cox are nothing more than Koch-funded shills who fight for tax cuts and weaker antitrust laws while drag queens read to our children. They are not 'based' and have no right to refer to themselves as such.

'Our culture is not your costume,' one popular right-wing Twitter personality quipped. Sohrab Ahmari, an American Conservative editor who advocates for 'political Catholicism,'tweeted'Im never using based again, now that these corporate schmucks have appropriated it.'

Ahmari also threw in some disparaging comments about Polumbos 'gigantic' head and Coxs 'tackily overspilling dcolletage' as they appeared in the announcement graphic.

I asked Polumbo (full disclosure: he and I are both affiliated with Young Voices) whether he expected this kind of reaction. Heres what he had to say:

Oh yeah, we anticipated pushback from the very-online nationalist crowd. Im not particularly bothered by it, although some of it has been ad-hominem and juvenile, and thats pretty pathetic, fan behaviour. We wont stoop to that. But the pushback is kind of the point. The nationalists want to redefine what it means to be conservative so that it resembles big government, socially conservative Elizabeth Warren economics more than Ronald Reagan. We still believe the future of the Right should be rooted in free markets, individual liberty, the Constitution, (and)limited government.

The term 'based' originated as slang for being high on crack. Perhaps theres some connection with 'freebasing', or smoking cocaine. From there, the terms definition expanded to cover all the strange and erratic behaviours typically associated with 'crackheads'. A modern equivalent might be to say someone is 'trippin' or 'tweakin'. These dont necessarily mean the person is under the influence of psychedelics or meth, only that theyre acting like it. 'Youre crazy,' spoken either with total dismissiveness or with a hint of admiration, would convey the same meaning.

'Based' began to take on its current connotation with rapper Lil B the Based God, who released his first album, Based Boys, in 2007. According to Lil B, 'Based means being yourself. Not being scared of what people think about you. Not being afraid to do what you wanna do.'

According to one online dictionary, the term, now a 'signal of power and swagger,' became associated with the online right in the 2010s as a synonym for 'politically incorrect'. Donald Trump was 'based' because he was willing to say things that annoyed the libs and then laugh at their outrage.

'Can you believe OrangeMan said X?!' the outraged soycuck shrieks. 'Lol, based,' the gigachad responds.

It seems to me that at this point, an earlier definition of 'based' unrelated to West Coast drug culture began to influence the terms use by the new right. 'Based' retained its sense of the manic, un-self-conscious energy of Trumps Twitter, but it also took on the the sense of being 'based on' or 'based in' something older and sturdier than the endless flux of liquid modernity. ric Zemmour is based. So is Viktor Orbn. Jailing pornographers, seizing the assets of the Ford Foundation, and going to Latin Mass with your nine kids and tradwife are all based. On the darker corners of right-wing Twitter, Rhodesia, Mussolini and overt expressions of sexism are also 'based'.

The prevailing definition of 'based' sits somewhere near the intersection of troll and trad.

Polumbo told me hes fully aware that hes going against the usual meaning of the term:

'While not our only mission, a crucial part of our project is to explicitly combat the nationalist conservative movement in a substantive and ideas-based way. We are redefining what it means to be based, whether they like it or not. Freedom is based. Catholic integralism and other forms of lite-theocracy are authoritarian and un-American.'

The post-liberal response, of course, would be that right-libertarianism, like progressivism, is a dominant ideology masquerading as a scrappy resistance. They are two faces of the same beast called liberalism. They divide the world between them: freedom in the boardroom and freedom in the bedroom.

Im not nearly the libertarian Polumbo is, but Im not a fully convinced post-liberal yet either. I think wokeness is a far greater threat than socialism. At the same time, I worry that any sort of post-liberal political project could lead to tyranny. The levels of social conservatism and, frankly, religiosity it demands simply dont have enough buy-in to win national elections.

Imagine the average Joe Rogan listener. Not the alt-right white nationalist monster sketched out in thinkpiece after thinkpiece, but the representative of the American median, the 'barstool conservative'.Hes economically agnostic, an admirer of entrepreneurship who distrusts large corporations. Socially, hes slightly to the left of centre. The idea of giving puberty blockers to kids freaks him out, but he has no interest in outlawing gay marriage or no-fault divorce. Hed be more likely than Polumbo to support trade protectionism and breaking up big tech, but Ahmari would have a hard time selling him on porn bans and blue laws.

Polumbo and Cox are betting that this voting bloc will be more open to Friedrich Hayek than to Thomas Aquinas. Their plan seems to be to stan capitalism while casting wokeness as a collectivist distortion of individual liberty rather than its natural outgrowth. Its possible, they promise, to combat the excesses of progressivism to be 'based' without throwing out many of the fundamental assumptions of American politics and culture.

It might work. If their attempt to reclaim 'based' succeeds, well know it has.

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Who will win the battle for 'based'? - Spectator.co.uk

Is Ron DeSantis Shaping Up to Be Trump 2.0 in 2024? – Truthout

A common refrain in the Trump era was that despite all of Donald Trumps racist, xenophobic ambitions, he was too incompetent to carry out a wholescale remaking of U.S. society. Theres no question his four years in office had a profound, disastrous effect on the people and communities his administration targeted immigrants, Muslims and trans people, just to name a few. Plus, his openly fascist rhetoric and sometimes actions significantly emboldened the far right. However, both his legislative agenda, and to a lesser extent his exercise of executive authority, were likely hampered by his lack of experience in elected office and overall laziness, lack of discipline and inattention to detail.

Many advocates understandably fear that Trumps eventual Republican presidential successor, whoever they are, could combine the most toxic elements of Trumpism with a greater degree of technocratic skill. A more-competent Trump could do even greater damage to U.S. political infrastructure, like the refugee resettlement system that Trump dismantled, that could take decades to rebuild. A more-competent Trump could also potentially shepherd through a legislative agenda that far exceeded Trumps, which consisted primarily of a standard-fare Republican tax cut for the wealthy.

Currently, the most likely GOP successor to Trump seems to be Florida Gov. Ron DeSantis, who regularly ranks as Republicans top choice in the 2024 presidential primary if Trump declines to run. Trumps polling lead over DeSantis appears to be narrowing in recent months, though any surveys this far out should be taken with a grain of salt. Everything in DeSantiss public record suggests that he poses a serious threat to democracy, the working class and marginalized communities. DeSantis recently signed into law some of the nations most stringent anti-critical race theory legislation, and ordered a review of the states university system to determine if professors were indoctrinating students into a stale ideology, clearly a reference to ideas ranging from anti-racism to socialism. Hes also pushing a bill that would prevent teachers and private businesses from making white people feel discomfort when learning about the history of racist oppression in the United States.

DeSantis has also recently staked out his position as more anti-vaccine, anti-booster than Trump himself. In December, the Florida governor refused to say if hed gotten a COVID booster when asked by Fox Newss Maria Bartiromo. Ive done whatever I did, the normal shot, DeSantis said. DeSantis got the Johnson & Johnson single dose in April, and stonewalled in October when asked if he would get a booster.

These statements, as well as DeSantiss rising stature in the national Republican Party, seem to have rankled Trump. In comments that were widely understood to be directed at DeSantis, Trump criticized politicians who wouldnt say if they had gotten a COVID booster shot. Many Republican politicians had in fact gotten the booster, but they dont want to say it. Because theyre gutless, Trump said on the far right One America News Network. You gotta say it whether you had it or not. (At a rally in December, Trump told the crowd that hed gotten the booster. They booed him in response.)

Several days later, Axios reported Trump has been calling DeSantis a dull personality in private meetings. Also, in that report, Trump aides claimed the former president was angry that DeSantis hasnt pledged not to challenge Trump in the 2024 GOP primary, should he run again. The New York Timess Maggie Haberman tweeted that shed heard similar frustrations from those in Trump world, who said Trump thought DeSantis should be showing him more deference.

DeSantis, for his part, has taken shots at Trump as well. On the conservative Ruthless podcast, the governor said one of his biggest regrets since taking office was not being much louder in opposing Trumps calls for soft lockdowns as COVID initially spread throughout the country and world.

DeSantis also appears to be shoring up support from conservative media stars to build a parallel track of public support, separate from his standing with Trump. On the anniversary of the January 6 storming of the Capitol, the Florida governor gathered nine prominent social media stars in Tallahassee, Politico reported. Blaze TVs Sara Gonzales told Politico it would be a mistake for DeSantis not to run, and that many conservatives were hungry for someone with the guts to speak for them without fear of repercussions, but also without the obvious baggage that Trump carries.

However, despite the simmering tensions between the two men, neither appears eager to engage in an all-out attack on the other. Last week, Trump told reporters that he has a very good relationship with DeSantis. He reminded them of his early support for the Florida politician when he was a relatively unknown congressman making a longshot bid for governor. DeSantis won the [gubernatorial] election the day I announced that I was going to give him my endorsement, Trump said on the call. He made similar comments to New York Times reporter Jeremy Peters in a forthcoming book, underscoring the degree to which Trump believes hes responsible for DeSantiss success. Look, I helped Ron DeSantis at a level that nobodys ever seen before, Trump told Peters.

Despite the emerging personal rivalry between DeSantis and Trump, the two are ideologically nearly identical, and also govern in a similar manner. DeSantis reportedly rules Florida with an iron fist and demands total fealty and loyalty, just as Trump does. Ron DeSantis is essentially the speaker of the House, the president of the Senate and the chief justice of the Supreme Court right now, one Republican legislator recently told Politico. Another GOP state legislator said in the same report that its well known you cant go against him. If you cross him once, youre dead.

In another clear echo of Trumpism, DeSantis is pushing for the creation of a new, so-called election police force. The new sub-agency, whose proposed official name is the Office of Election Crimes and Security, would operate under the Department of State, which is controlled by the governor. Voting rights advocates are understandably alarmed, citing the extremely low levels of deliberate voter fraud and arguing that the goal of the new sub-agency is to depress turnout, particularly among Black voters and other groups historically targeted for harassment.

Also worrisome is a new proposed congressional district map released by DeSantiss office, an incredibly rare phenomenon. DeSantis map would cut in half the number of African American districts from four on current proposed congressional maps to two, while boosting the number of seats Donald Trump would have won in 2020 to 18 from the 16 on the map currently being considered by the GOP-led Florida Senate, Politico reported.

The 2024 primary will semi-officially start this year, after the November midterms. Trump hasnt announced whether hell run, but all signs suggest that he will. DeSantis is a young, popular, far-right politician who doesnt command the base of the party like Trump does, but comes closer than any other potential challenger at the moment. Ever since Trumps upset victory in 2016, those on both the right and the left have wondered what Trumpism without Trump might look like. In DeSantis, we could be seeing an answer.

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Is Ron DeSantis Shaping Up to Be Trump 2.0 in 2024? - Truthout

H+ 3: Radical Life Extension? Cybernetic Immortality? Or Resurrection of the Body? – Patheos

Which do you prefer: radical life extension? Cybernetic immortality? Resurrection of the body? Or, just death as endless oblivion?

Transhumanism also known as Humanity Plus or H+ plans to solve all human problems with advances in AI (Artificial Intelligence). One H+ goal is to cure death. Without the threat of death, we could elect to live forever. Either in your body or in disembodied form. Are you interested?

Here are four options. Check the box you prefer.

1 Natural death as the end of all conscious existence.

2 Radical Life Extension (RLE) in your present body.

3 Cybernetic Immortality (CI): your consciousness lives on in the computer cloud.

4 Resurrection of the body as promised in 1 Corinthians 15.

In this post the public theologian will explore the contrast between radical life extension and resurrection in light of transhumanist promises. Whether via Radical Life Extension in the body or Cybernetic Immortality in disembodied form, enhanced human intelligence and consciousness have an unending future. So we are promised. Should we trust this techno-promise? Or, should we trust Gods promise of resurrection from the body, the soma pneumatikon? What does it take for us greet the end of life with contentment?

Tracy Trothen and Calvin Mercer are the indefatigable chroniclers of the interaction between Religion and the Technological Future.

Here, in this post, I offer the third in a series of column posts tackling the religious, ethical, and social concerns raised by genetic engineering, AI (artificial intelligence), IA (intelligence amplification), transhumanism, and posthumanism. First, we asked: H+ 1: Is AI a shortcut to virtue? Or to holiness? Then, secondly, we examined varieties of religious transhumanism in the post: H+ 2: The Transhuman, the Posthuman, and the Truly Human. Here we take up one specific issue: how do we compare multiple scenarios for life extension and immortality?

Death is natural, right? Death is the irreversible cessation of the processes that keep the body working, observes physicist and theologian John Polkinghorne. Death really is the end. Its annihilation (Polkinghorne 2019, 49). With death comes oblivion.

Yet, the prospect of falling from existence into non-existence at the moment of death issues forth in anxiety. In the anxiety of having to die nonbeing is experienced from the inside, says theologian Paul Tillich. This anxiety is potentially present in every moment. It permeates the whole of mans being; it shapes soul and body and determines spiritual life; it belongs to the created character of being quite apart from estrangement and sin(Tillich 1951-1963, 1:193-194).

Is death a disease that needs to be cured? Bioethicist Gilbert Meilaender denies that aging and death belong in the disease category. Unlike disease, he says, aging is a natural stage of life that seems built in (Meilaender 2013, 2). We should live within the limits of death and aging by cultivating the virtue of patience.

If you check this first option, you elect to end your embodied consciousness and to drop from being into nonbeing. Can you be patient with this?

Transhumanists define death as a disease. Like other diseases, medical science will cure death. Then, we can live in our present bodies indefinitely.

One clarification. Lets distinguish between the aim of longevity research (LR) and radical life extension (RLE). LR is being pursued by the Buck Institute, Geron Corporation, Michael D. Wests Therapeutics, and other laboratories. Both LR and RLE belong to the prolongevity club. Note that LR is modest while H+ RLE is dramatic. Big bucks finance both. (Photo: Aubrey de Grey)

Oxford transhumanist Nick Bostrom sells stock in both LR and RLE because of the potentially enormous returns, in terms of human welfare, to investment in biogerontological research if it could lead to a further significant extension of the human health span.

Cambridge geneticist and biogerontologist Aubrey deGrey proclaims that science must triumph over what has hitherto been natures province, namely, aging and natural death. There is a good chance aging can be entirely defeated within the next few decades, de Grey writes. The speed of speed is speeding up, meaning that the relevant laboratory research is advancing at an increasingly rapid pace. The longevity escape velocity or LEV is spiraling upward(DeGrey 2009, 21).

In the race for a life of a thousand years, the runner still needs to dodge incidental death threats. Referring to RLE as practical immortality, Calvin Mercer and Tracy Trothen alert us to remain alert. Practical immortality means that one will not die from internal biological causes that have been associated with aging, but one could still die of such things as accidental causes, a new infectious disease, natural disasters, or a cosmic event(C. a. Mercer 2021, 72).

Radical life extension consists of the prolongation of terrestrial embodied living as we have known it minus the deterioration of aging and the portent of an end to life. Unless we get run over by a bus or shot in a war, we can expect over the centuries an unending number of stints on the altar guild or picnics with the PTA. Are you ready for this?

RLE is embodied immortality. Cybernetic immortality is disembodied.

The aim of the cybernetic prolongevists is to dislodge our consciousness and our intelligence from our biological substrate and upload it into a computer. As long as the computer battery remains charged, we will live in a disembodied electronic cloud virtually forever. Immortality can be attained here by uploading the information stored in our brain, a process called whole brain emulation.

How might this work? Cybernetic immortality could be attained, according to computer whiz Ray Kurzweil, because intelligence is not dependent upon our biological substrate. Kurzweil, transhumanist in mind-set even if not in name, defines intelligence as an information pattern and, as an information pattern, our intelligence could be transferred from our brain to a computer. The current substrate that houses this information pattern is our body, the eighty to ninety billion neurons in our brain. Could this neuronal information be transferred to a disembodied digital substrate? Yes, say transhumanists.

Our intelligence can live on in an enhanced form even when extricated from our bodies and placed in a computer. Uploading a human brain means scanning all of its salient details and then reinstantiating those details into a suitably powerful computational substrate. This process would capture a persons entire personality, memory, skills, and history (Kurzweil 2005, 198-199). From Kurzweils forecast of the Singularity by the year 2045, todays 2045 Initiative has begun to organize the research.

Kurzweil, like others in the H+ movement, plans to derive Homo cyberneticus from modifying Homo sapiens. Once our minds are uploaded, we will become a new species, a posthuman species. The present generation giving birth to the future posthuman species is made up of the transhumanists.

Have we seen this before? Remember Ren Descartes (1596-1650)? Descartes was a substance dualist. Our body is a physical substance while are mind or soul is a spiritual substance. By performing a soulechtomy, God could extract the soul from its physical housing, said Descartes. Now the transhumanist can do what before only God could do, allegedly.

Would you like to live indefinitely bodiless in the computer cloud?

Is it realistic to anticipate the defeat of death and unending finite life? This question must be addressed on two levels, one psychological and the other scientific.

A curious argument has broken out over the psychological acceptance of living indefinitely. Transhumanists are convinced that nobody wants to die. The transhumanists are probably right on this point.

But, does it follow that everybody wants to life forever? This, it turns out, is a matter of debate. University of Liverpool philosopher Michael Hauskeller believes the transhumanists commit the Immortality Fallacy here. If you ask people whether they want to die, most of them will indeed deny it. However, if you as, the same people whether they want to go on living forever, you may find that most will deny this too. It is quite possible that a person does not want to die and still does not want to live forever(Hauskeller 2013, 89).

In short, the question of living forever is independent of the question of death. That a person does not normally want to die does not imply that the same person does not ever want to die (i.e. in the future), adds Pablo Garca-Barranquero at Universidad de Mlaga(Garcia-Barranquero 2021, 183).

Now we ask scientifically, is RLE realistic? Probably not. LR is not likely to lead to RLE, at least according to Michael Shermer, writing in Scientific American, Radical Life Extension Is Not around the Corner(Shermer 2016, 84).

If RLE is not realistic, might cybernetic immortality be realistic? Scientifically speaking, probably not. Kurzweils presupposed theory that the human mind is an information pattern extractible from the brain does not square with what is known about mind, intelligence, and consciousness.

First, human intelligence is not limited to the brain. It is distributed throughout the body by the nervous system.

Second, all thinking includes biological stimulus and interaction, much of it with the surrounding world(Peters, Where Theres Life Theres Intelligence 2017). Intelligence is relational. dependent on the relationship of the body to the world. Our intelligent minds are inextricably embodied and relational.

Computer scientist and theologian Noreen Herzfeld, for example, emphasizes that we cannot be who we are except in relationship to others and to our natural world (Herzfeld 2002, 86-95). In short, disembodied consciousness or mental activity does not make scientific sense. (Photo: Noreen Herzfeld)

The acknowledgment of embodiment makes neuroscientists critical of the concept of cybernetic immortality. Kurzweils knowledge of neuroscience is imply inadequate to the task at hand.He mistakes the striatum for cortex and apical dendrites for axons, belies the cognitive contributions of the basal ganalia.Yet he [Kurzweil] has the unerring belief of the prophet or the fool.(Koch 2013, 760). Are transhumanists fools?

Would you be a prophet or a fool to check the cybernetic immortality box?

Caution. Some in faith communities react spasmodically: H+ should immediately be shunned! Why? Because H+ represents human hubris. Because H+ constitutes playing God like Frankenstein.

Rather than simply kicking our H+ friends, however, I recommend pausing to take a deep breath. Then allot a few moments to compare and contrast RLE and CI with the biblical promise of resurrection.

When we turn to biblical Christianity, the question of immortality does not stand alone. Standing right alongside of resurrection is the forgiveness of sin and redemption from evil. Death constitutes a cleansing, so to speak. We become washed in the blood of the lamb.

Before approaching omega, lets return for a moment to alpha. Lets return to the expulsion of Adam and Eve from the Garden of Eden in Genesis 2 and 3. Why did God expel Adam and Eve from the garden and place an angel with the fiery sword next to the No Admittance sign? Was God throwing a temper tantrum in response to the eating of the forbidden fruit? No. Adam and Eve had certainly eaten from the Tree of the Knowledge of Good and Evil, but they had not yet tasted the fruit from another important tree in the garden. That was the Tree of Life. Had they eaten from the Tree of Life, then they would live forever (Genesis 3:22). That is, we human beings would live forever in our sin, never able to escape the vicissitudes of betrayal, victimization, suffering, agony, and anxiety anticipating all of this. Evil would then have to be endured everlastingly. Here is the point: death is Gods gift. By sending Adam and Eve to their death, God was graciously bestowing escape from everlasting distress and misery.

What about sin and evil? Our transhumanist friends are as cognizant of sin as a public monument is cognizant of the pigeon excreta that adorns it. That is, they ignore it.

Here is the implication: RLE and CI would perpetuate unendingly human malevolence and victimization. New Testament historian, N.T. Wright reminds us: The kingdoms of the world rage against the kingdom of God; the problem of evil grows teeth and claws, leaping out with a snarl from the debating halls of the philosophers and on to the stage of the real world, turning gardens into deserts and human lives to dust and ashes.Evil is alive and powerful(Wright, Evil and the Justice of God 2006, 38).

It is decisive, then, in Christian theology that when we die we really die to this world. Death is total. No soulecthtomy. No mind extraction. No residuals from this life are carried on. Or, more precisely, the sins and sufferings of this life die with us.

The purpose and meaning of our individual and social lives, to be sure, come to fulfillment in our resurrection. We undergo both discontinuity and continuity as we pass through death into resurrection.

In order to convey this point, Saint Paul employs the seed analogy. A seed in the hand looks dead. Its dry. Inert. But, when we plant it in the garden with fertilizer and water, it springs up with flower or fruit, with beauty or nourishment. This describes, at least in part, what it is like for you and me to die and be raised by God.

So it is with the resurrection of the dead. What is sown is perishable (corrupt, pthora), what is raised is imperishable (incorrupt, aphtharsia).It is sown in dishonour (atimia), it is raised in glory (doxa). It is sown in weakness (astheneia), it is raised in power (dynameis).It is sown a physical body (soma psychikon), it is raised a spiritual body (soma pneumatikon). If there is a physical body, there is also a spiritual body. (I Corinthians 15:42-44).

Note Pauls term for physical body, soma psychikon. That is an ensouled body, a body with a mind. Body and soul along with our intelligence die. Kaput. Kafinished. Over. No cybernetic immortality here.

Paul stresses that what is redeemed is healed, renewed, transformed. In the resurrection we will be made ready by Gods grace for eternal life. Thats what soma pneumatikon indicates, a spiritual body.

To be spiritual does not mean to be ethereal. The resurrected body is still a body, only healed.

Let me mention again that I am editing a book with Arvin Gouw and Brian Patrick Green,Religious Transhumanism and Its Critics(Roman & Littlefield, 2022). Watch for it.

Now, which box did you check?

Our transhumanist friends will attempt to make us immortal so we can live forever in this world, this fallen world. The biblical eschatological vision, in contrast, anticipates a transformation of the world coincidental with our resurrection. Cosmic eschatology and personal eschatology coincide. Eschatology has always to be both universal and individual, says Wolfhart Pannenberg rightly (Pannenberg 1991-1998, 3:543). The consummation of Gods redemptive work for the whole of the cosmos provides the backdrop for our personal resurrection from the graves oblivion. Jesus Easter resurrection is a prolepsis of your and my resurrection.

Reformed theologian and bioethicist Ronald Cole-Turner, sets up the parallel between H+ and the biblical promise. Technology offers to give us what we want, or at least what most of us think we wantlonger life, youthful bodies, greater health, and mental ability. Christianity invites us to give up what we want, indeed to give up life itself, as the one condition for real life(Cole-Turner 2009, 58).

The New Testament message is that the God who raised Jesus from the dead on the first Easter will greet us on the other side of deaths darkness. The light beyond deaths darkness is the light of the world, the brilliance of a redeemed cosmos. This is the gospels call to trust, to trust when we cannot be in control.

Ted Peters directs traffic at the intersection of science, religion, and ethics. Peters is a professor at the Graduate Theological Union (GTU), where he co-edits the journal, Theology and Science, on behalf of the Center for Theology and the Natural Sciences (CTNS), in Berkeley, California, USA. He is author of Playing God? Genetic Determinism and Human Freedom (Routledge, 2nd ed., 2002) and editor of AI and IA: Utopia or Extinction? (ATF 2019). Watch for Teds forthcoming book, The Voice of Public Christian Theology (ATF 2022). Visit his website: TedsTimelyTake.com.

Cole-Turner, Ronald. 2009. Extreme Longevity Research: A Progressive Protestant Perspective. In Religion and the Implications of Radical Life Extension, by ed Calvin Mercer, 50-62. New York: Macmillan Palgrave.

DeGrey, Aubry. 2009. Radical Life Extension: Technological Aspects. In Religion and the Implications of Radical Life Extension, by eds. Derek F. Mather and Calvin Mercer, 13-24. New York: Macmillan Palgrave.

Garcia-Barranquero, Pablo. 2021. Transhumanist Immortality: Understanding the Dream as a Nightmare. Scientia et Fides 9:1 177-196.

Hauskeller, Michael. 2013. Better Humans? Understanding the Enchantment Project. London: Routledge.

Herzfeld, Noreen. 2002. In Our Image: Artificial Intelligence and the Human Spirit. Minneapolis MN: Fortress.

Koch, Christoph. 2013. The End of the Beginning for the Brain. Science 339:6121 759-760.

Kurzweil, Ray. 2005. The Singularity if Near: When Humans Transcend Biology. New York: Penguin.

Meilaender, Bilbert. 2013. Should We Live Forever? The Ethical Ambiguities of Aging. Grand Rapids MI: Wm B Eerdmans.

Mercer, Calvin. 2015. Whole Brain Emulation Requires Enhanced Theology, and a Handmaiden. Theology and Science 13:2 175-186.

Mercer, Calvin, and Tracy Trothen. 2021. Religion and the Technological Future: An Introduction to Biohacking, Artificial Intelligence, and Transhumanism. New York: Macmillan Palgrave.

Pannenberg, Wolfhart. 1991-1998. Systematic Theology, 3 Volumes. Grand Rapids MI: Wm B Eerdmans.

Peters, Ted. 2017. Where Theres Life Theres Intelligence. In What is Life? On Earth and Beyond, by ed Andreas Losch, 236-259. Cambridge: Cambridge University Press.

Polkinghorne, John. 2019. What can we hope for? Cambridge : Cambridge University Press.

Shermer, Michael. 2016. Radical Life Extensiion is NOT around the Corner. Scientific American 316:10 84.

Tillich, Paul. 1951-1963. Systematic Theology. 1st. 3 Volumes: Chicago: University of Chicago Press.

Wright, NT. 2007. Cosmic Future: Progress or Despair? In From Resurrection to Return: Perspectives from Theology and Science on Christian Eschatology, by Christine Ledger, and Stephen Picard, eds James Haire, 5-31. Adelaide: ATF Press.

. 2006. Evil and the Justice of God. London: SPCK.

Young, Simon. 2006. Designer Evolution: A Transhumanist Manifesto. Amherst NY: Prometheus Books.

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H+ 3: Radical Life Extension? Cybernetic Immortality? Or Resurrection of the Body? - Patheos

Tuning protein half-life in mouse using sequence-defined biopolymers functionalized with lipids – pnas.org

Significance

Functionalization of proteins and biopolymers with chemical modifications can be utilized to alter their chemical and biophysical properties. In contrast to traditional chemical functionalization strategies, the use of nonstandard amino acids enables precise positioning of functional groups. Here, we report that multisite conjugation of fatty acids, at precise sites harboring genetically encoded nonstandard amino acids with bioorthogonal chemical handles, can be employed to tune the half-life of proteins in a mouse model. This programmable approach could offer a technical foundation for the modification of protein and peptide therapeutics to improve their efficacy or pharmacokinetic profile (e.g., to prevent rapid clearance and reduce frequency of administration).

The use of biologics in the treatment of numerous diseases has increased steadily over the past decade due to their high specificities, low toxicity, and limited side effects. Despite this success, peptide- and protein-based drugs are limited by short half-lives and immunogenicity. To address these challenges, we use a genomically recoded organism to produce genetically encoded elastin-like polypeptideprotein fusions containing multiple instances of para-azidophenylalanine (pAzF). Precise lipidation of these pAzF residues generated a set of sequence-defined synthetic biopolymers with programmable binding affinity to albumin without ablating the activity of model fusion proteins, and with tunable blood serum half-lives spanning 5 to 94% of albumins half-life in a mouse model. Our findings present a proof of concept for the use of genetically encoded bioorthogonal conjugation sites for multisite lipidation to tune protein stability in mouse serum. This work establishes a programmable approach to extend and tune the half-life of protein or peptide therapeutics and a technical foundation to produce functionalized biopolymers endowed with programmable chemical and biophysical properties with broad applications in medicine, materials science, and biotechnology.

A major goal of synthetic biology is to harness biological systems to produce valuable products, such as new therapeutics, renewable chemicals, and functionalized materials. In the case of proteins, the native translation process uses information encoded in DNA to guide their template-directed production at monomeric precision, albeit limited to the chemistry of the 20 natural amino acids. Work in genetic code expansion with nonstandard amino acids (nsAAs) has expanded the chemical palette of biology through the template-directed biosynthesis of proteins with synthetic chemistries (1). To date, such work has been limited to only one or a few instances of site-specific incorporation of nsAAs per protein, constraining biopolymer synthesis to tag-and-modify approaches or simple protein decorations. Recent advances include an increase in the number and chemical diversity of nsAAs (1), the development of highly active translation machinery for efficient incorporation of nsAAs into proteins (2, 3), and engineered strains of Escherichia coli with recoded genomes possessing open coding channels that can be dedicated to the incorporation of nsAAs (46). Together, these advances enable multisite incorporation of nsAAs to endow proteins and sequence-defined biopolymers with new chemical and biophysical properties.

An active area of interest for the use of nsAAs is to enhance the functionality of protein and peptide pharmaceuticals. They represent a versatile and fast-growing class of biological therapeutics (7, 8) that are particularly attractive as potential pharmaceuticals due to their high specificity, high activity and, in the case of peptides, rapid tissue penetration (7). However, major barriers prevent the widespread clinical use of many peptide or protein-based therapeutics (7): 1) the need to administer them by injection, 2) their rapid clearance by the kidneys, and 3) their rapid proteolytic degradation. As a result, these pharmaceuticals must be frequently administered at high doses, leading to a peak-and-valley pharmacokinetic profile. These characteristics can negatively affect therapeutic efficacy, can cause undesirable side effects with reduced patient compliance (912), and can trigger an immune response, including the induction of a neutralizing antibody response (13, 14).

To address these challenges, proteins and peptides are frequently functionalized to extend their half-life and improve immunotolerance. A widely adopted strategy is the conjugation of poly(ethylene glycol) (PEG), which increases the radius of the protein and reduces proteolytic cleavage, and consequently reduces clearance (15). However, the development of alternatives to PEGylation remains important, as PEGylation does not always offer the desired effect on pharmacokinetics, and in certain cases, safety concerns about its immunogenicity and accumulation in tissues have been raised (1618). An alternative strategy is to conjugate or fuse the therapeutic protein or peptide to serum proteins with long half-lives, such as serum albumin, antibodies (e.g., full-length or fragments of IgG), or blood components, such as red blood cells (17, 18). Similarly, many approaches use chemical moieties or peptides to promote noncovalent binding interactions to the same serum proteins and complexes in order to extend half-life (1720). One effective and safe option is the use of fatty acids (FAs) to promote binding to serum albumin. For example, insulin and glucagon-like peptide-1 (GLP-1) conjugated with a single FA are clinically used to treat diabetic patients (2123).

A major hurdle to the development of functionalized therapeutics is to selectively and predictably modify the protein while maintaining bioactivity. Conventional strategies for PEGylation and functionalization with chemical moieties utilize chemistries that modify the target protein at their termini, or at residues with reactive side-chains (24). The functionalization at C or N termini can be highly selective and predictable, but it can reduce bioactivity and is thus incompatible with many proteins. In contrast, modifications at reactive side-chains (e.g., cysteine or lysine) is less restrictive, but it can be difficult (or practically impossible) to identify unique reactive sites in the peptide sequence for site-specific conjugation. To address this problem, amino acids in the protein are typically mutagenized, which can result in reduced bioactivity. Recently, nsAAs have been successfully employed for modification of proteins and peptides, offering bioorthogonal chemistries for functionalization at predetermined positions within the protein (2426). For example, human growth hormone (hGH) and fibroblast growth factor 21 (FGF21) were site-specifically PEGylated, prolonging their function through extended serum half-life in clinical trials (27, 28). In other cases, site-specific lipidation at a single nsAA was shown to extend half-life in mouse models (29, 30). Although these approaches have improved protein half-life, typically these designs are constrained to a single instance of the nsAA, which limits the versatility and tunability (e.g., customized range of half-lives) of these functionalized peptides and proteins.

In this study, we present a synthetic biology platform to biosynthesize proteinpolymer fusions with sequence-defined conjugation sites for multisite lipidation in order to extend and tailor the half-life of proteins invivo. Specifically, we encoded up to 10 instances of the nsAA para-azidophenylalanine (pAzF) in elastin-like polypeptide (ELP) fusion proteins at high yields in an engineered bacterium with a recoded genome (4), and demonstrate the ability to precisely control the position and number of FAs per biopolymer. We found that the number of FAs per protein is strongly correlated with the binding affinity to serum albumin, enabling us to tune the invivo serum half-life of proteins without accumulation in organs or eliciting an inflammatory response in mouse. These advances could be applied to extend and tune the half-life of protein or peptide therapeutics and establish a technical foundation to produce sequence-defined programmable biopolymers endowed with bespoke chemical and biophysical properties with broad applications in medicine, materials science, and biotechnology.

To enable the biosynthesis of sequence-defined synthetic biopolymers with template-directed conjugation sites, we utilized a recently described synthetic biology expression system that allows efficient incorporation of nsAAs (e.g., pAzF) at UAG codons (Fig. 1A) (2). This system possesses two unique properties. First, the expression host is the genomically recoded organism (GRO) (4), an E. coli MG1655 derivative, in which all instances of UAG stop codons were recoded to synonymous UAA codons, followed by the deletion of release factor 1 (RF1). This GRO establishes an open codon by eliminating competition between an orthogonal tRNACUA/aminoacyl tRNA synthase (aaRS) pair and termination at UAG codons by RF1. Second, aaRSs evolved for aminoacylation with nsAAs typically have significantly reduced activities compared to native enzymes, resulting in low levels of nsAA-tRNA and low yields for proteins with multiple instances of an nsAA (31). Here, we use a tRNACUA/aaRS derived from Methanocaldococcus jannaschii that was evolved for enhanced activity, enabling efficient multisite incorporation of nsAAs into proteins (2). Together, this expression system enables the biosynthesis of polypeptide polymers with multiple pAzF residues at high yields and accuracy.

Biosynthesis and functionalization of genetically encoded biopolymers for half-life extension of proteins. (A) Site-specific multisite incorporation of pAzF at UAG codons in the GRO. All 321 TAG codons in E. coli were genomically recoded to TAA. To create the GRO, RF1 was deleted. The canonical amino acids and pAzF are shown as black and red circles, respectively. The TAG codon is converted into a sense codon for multisite incorporation of pAzF. (B) Schematic of the ELP-protein with 10 pAzF residues. The chemical structure of pAzF and the sequence of a single ELP repeat are shown. (C) Functionalization of azido groups in ELPs through copper(I)-mediated click chemistry with alkynyl palmitic acid. (D) Functionalized biopolymers are characterized in mice to study impact on half-life.

To study the effect of the number of FA conjugates on the invivo serum half-life, we chose to introduce the nsAA in an ELP with 10 consecutive pentadecapeptide repeats for functionalization. ELPs have previously been fused to active therapeutic peptides for a variety of indications, including type 2 diabetes and cancer (32), and serve as a versatile module to alter their pharmacokinetics. Within each repeat of the ELP, we encoded either a tyrosine or a pAzF residue at a designated guest residue position (henceforth named the target residue) (Fig. 1B), such that the genetic template controls the number and position of pAzF residues in the ELP-GFP. In turn, the bioorthogonal copper(I)-catalyzed azide-alkyne Huisgen cycloaddition (click-chemistry) reaction between pAzF residues and palmitic acid alkynes (with an terminal alkyne, such that the carboxyl group is exposed) ensures site-specific conjugation (Fig. 1 C and D). Alkynyl palmitic acid was used for functionalization because it had previously been shown to strongly promote binding to albumin (23).

To evaluate if we could produce proteins with a genetically controlled number of FAs, we expressed ELP-GFP with 0, 1, 5, or 10 UAG codons at a yield of 70 mg/L (Fig. 2A and SI Appendix, Fig. S1). To carefully examine the fidelity and efficiency of each step in our system, we performed quantitative mass spectrometry (MS) analysis of the ELPs digested with thermolysin, which liberates each of the 10 constituent ELP units. To account for differences in ionization efficiency between the different peptide species, ion counts were quantified using a standard curve for each peptide (SI Appendix, Fig. S2). We first evaluated the efficiency of pAzF incorporation and found that the abundance of ELP units with pAzF was directly proportional to the number of UAG codons in the construct (SI Appendix, Fig. S3). Consistent with prior work (2), when all 10 ELP units contained a UAG codon, we detected minor (<5%) tyrosine misincorporation.

Analysis of the purity and bioactivity of multisite lipidated biopolymers. (A) Schematic representation of ELP-GFP reporter constructs with 1, 5, or 10 pAzF residues. Target positions for pAzF are indicated in red. (B) Relative abundance of detected nonstandard amino acids at target residues of ELP units based on quantitative MS. Treatment with ISAz selectively converts reduced pAzF residues, pAF, back into pAzF. (C) Click-chemistry with FA alkynes functionalizes all pAzF, but not pAF, residues (n = 3, error bars: mean SD). (DG) Intact MS of full-length ELP(FA)-GFP after click-chemistry with (blue) or without (black) ISAz treatment. (H) Activity of recombinant trx, trx-ELP(10pAzF), and trx-ELP(10FA) at protein quantities ranging from 25 to 150 pmol per well. (I) Effect of HSA on the activity of recombinant trx, trx-ELP(10pAzF) and trx-ELP(10FA) using 100 pmol of each protein and 500 pmol HSA. Data are normalized to the activity of each protein without HSA (n = 3, error bars: mean SEM). *P < 0.05, **P < 0.01.

While examining the fidelity of pAzF incorporation, we observed significant levels of para-aminophenylalanine (pAF) (Fig. 2B), the reduced form of pAzF, which cannot participate in click-chemistry. In our system, pAF is the result of pAzF reduction (2, 33), and causes significant impurities and heterogeneity in the final preparations if left unresolved. To overcome this impurity, we developed a method to selectively recover pAzF from pAF with the diazotransfer reagent imidazole-1-sulfonyl azide (ISAz) (Materials and Methods) (34). We previously demonstrated that this approach enabled effective conversion of pAF residues to pAzF, without introducing azides at other primary amines found in ELP-GFPs (34). Consistent with this prior work, after treatment with ISAz we observed less than 5% of pAF via quantitative MS in each of the digested ELP-GFP constructs (Fig. 2B).

We then used click-chemistry to attach alkynyl palmitic acid at the precise positions where pAzF was encoded and assessed the purity of each ELP-GFP construct. These functionalized constructs are denoted as ELP(nFA)-GFP, where n indicates the number of UAG codons encoding pAzF in the template. We observed that all pAzF residues were converted to FA conjugates and no further reduction to pAF was detected during this reaction (Fig. 2C), emphasizing the high efficiency of this conjugation strategy. To complement the quantification at the peptide level, we used MS of the intact protein to evaluate the purity of the products (Materials and Methods). We consistently observed one dominant peak at the expected mass after ISAz treatment, whereas untreated samples demonstrated heterogeneous modification of the ELP-GFP (Fig. 2 DG). For example, the ELP(10FA)-GFP without ISAz treatment showed multiple distinct peaks corresponding to an impure biopolymer with variable number of FAs. The peak profile correlates with a binomial distribution determined by the availability of pAzF residues (SI Appendix, Fig. S4) and suggests pAzF reduction is probabilistic.

Finally, we evaluated if the addition of multiple FAs per protein would impair solubility of the resulting constructs. The solubility of unmodified ELP-GFP and ISAz-treated proteins with 1, 5, or 10 FAs were determined by dynamic light scattering (DLS) analysis. All constructs, before and after FA conjugation, were soluble (>99% by volume) and did not self-assemble in solution (SI Appendix, Fig. S5). Together, these results demonstrate that the genetically controlled placement of pAzF and chemical regeneration of reduced pAF residues enable the programmable and robust functionalization of biopolymers at multiple sites.

We evaluated the effect of ELP and FA conjugation of peptide bioactivity on the activity of two proteins. We first quantified the effect of FA conjugation on GFP fluorescence, and found that it is reduced by 14 to 25% (SI Appendix, Fig. S6). In addition, to examine the effect of ELP fusion and FA conjugation on enzymatic activity, we produced and characterized thioredoxin (trx)-ELP fusion proteins. We evaluated the activity of recombinantly produced trx, trx-ELP(10pAzF), and trx-ELP(10FA), after ISAz treatment. Similar bioactivity was observed for trx and trx-ELP(10pAzF), while trx-ELP(10FA) retained greater than 50% bioactivity (Fig. 2H). In addition, we evaluated the effect of human serum albumin (HSA) binding on the activity of these proteins. To this end, we performed an activity assay in the presence of fivefold excess HSA concentration, and found that HSA binding reduced the activity of trx-ELP(10FA) by 50%, while activity of trx and trx-ELP(10pAzF) were not significantly reduced (Fig. 2I). Although FA conjugation and HSA binding may partially reduce bioactivity, these analyses demonstrate that posttranslational functionalization with FAs is compatible with bioactive proteins.

Since prior work with single FA conjugations of insulin showed that serum half-life is correlated with the binding affinity to albumin (23), we hypothesized that multisite lipidation of ELP-GFPs would significantly enhance binding affinity to mouse serum albumin (MSA), and consequently extend serum half-life, compared to a single conjugated FA. To study the impact of increasing the number of FAs, we analyzed ELP-GFP constructs (both with and without ISAz treatment) with surface plasmon resonance (SPR). The KD values of our constructs were estimated based on the steady-state binding (Table 1). There was no detectable binding between MSA and the negative control without conjugated FAs [ELP(0FA)-GFP]. For untreated biopolymers, we found that the KD with a single FA, ELP(1FA)-GFP (KD = 126 32 M), was lowered 12- to 45-fold for ELP(5FA)-GFP (KD = 10.4 4.0 M) and ELP(10FA)-GFP (KD = 2.8 0.2 M), respectively. For the ISAz-treated biopolymers, we observed much stronger binding overall: treated ELP(1FA)-GFP presented a KD of 25.9 7.1 M, and an increase to 5 and 10 FAs per protein further lowered the KD to 4.0 1.6 M and 2.22 0.03 M, respectively. These data indicate that the affinity for MSA is strongly enhanced by conjugation of multiple FAs per protein and confirm that the binding affinity is correlated with the number of FAs.

Binding affinity of ELP-GFP constructs for serum albumin

We next determined if the tighter binding affinity is translated to prolonged half-life in C57BL/6J mice. A total of 50 g of each protein variant (10 M in phosphate-buffered saline [PBS]) was injected intravenously and blood was collected after 1, 4, 8, 16, and 24 h, followed by daily collections for 7 d. The blood levels of ELP(nFA)-GFP constructs were measured using a GFP-specific ELISA, and their pharmacokinetic profiles were calculated (SI Appendix, Fig. S7 and Table S1). We observed a striking 16- to 19-fold increase in half-life from 1.7 h for ELP(0FA)-GFP to 28 to 33 h for ISAz-treated ELP(5FA)-GFP and ELP(10FA)-GFP, as well as for untreated ELP(10FA)-GFP (Fig. 3A). Notably, when the same constructs were injected subcutaneously, we observed a delayed peak concentration, but the half-lives were equivalent to intravenous injections (SI Appendix, Figs. S8 and S9). Furthermore, these data show that the half-life of tight binding ELP-GFP constructs with multiple FA conjugates approaches the half-life of MSA in mice (35 h) (35), and is similar to the half-life of 28 h reported for proteinMSA fusion proteins (36).

In vivo characterization of lipidated biopolymers in mouse. (A) Serum half-life measurements of lipidated biopolymers with or without treatment with ISAz. Measurements were collected after a single intravenous injection of 50 g biopolymer in C57BL/6J mice. ELP(0FA)-GFP after ISAz treatment was not measured (n = 4, error bars: mean SD). (B) Correlation between the KD and half-life of lipidated biopolymers with or without ISAz treatment. The horizontal, black dotted line shows the half-life of MSA, the dashed gray line shows model predictions (n = 4 to 8, error bars: mean SD, n.d. = not detected). (C) Distribution of biopolymers in mouse organs, 3 and 48 h after intravenous injection of Alexa Fluor 648labeled ELP(0FA)-GFP or ELP(10FA)-GFP. The biopolymers were treated with ISAz. The data are representative of four independent measurements. (D) Quantification of average Alexa Fluor 648 intensity for organs shown in C (n = 4, error bars: mean SEM). For each organ separately, a one-way ANOVA was used to determine whether the differences between the means of the five treatment groups were statistically significant. After multiple testing correction, *P < 0.05 and **P < 0.005. (E) Serum concentration of select inflammatory cytokines at 3 and 48 h after injection. Endotoxin (100 g) and PBS were used as positive and negative controls, respectively. Measurements below the lower limit of detection (20 pg/mL) or above the upper limit of detection (5,000 pg/mL) are plotted at their limit of detection (n = 3, error bars: mean SD).

Finally, we note that the observed KD values can be approximated by dividing the KD of ELP(1FA)-GFP (ISAz-treated) by the average number of conjugated FAs (SI Appendix, Fig. S10). This correlation has two key implications. First, biopolymers with high numbers of FA conjugates [i.e., ISAz-treated ELP(5FA)-GFP, and both treated and untreated ELP(10FA)-GFP] will have similar binding affinities to albumin, and therefore also similar half-lives. Second, tuning of the binding affinity and half-life is most pronounced at lower numbers of conjugated FAs. In the case of ISAz-treated ELP(10FA)-GFP, we found a small decrease (although not statistically significant) in half-life compared to treated ELP(5FA)-GFP and untreated ELP(10FA)-GFP. We hypothesize that denser packing of the 10 FAs does not improve, or may even reduce, the availability of FAs for albumin binding, highlighting the value of being able to precisely control the number of FAs per protein.

We computationally modeled the system to gain a deeper understanding of the correlation between the binding affinity and half-life (SI Appendix, Supplementary Information Text and Fig. S11). In brief, a set of ordinary differential equations describes the binding and release of ELP-GFP from albumin as a function of the KD, as well as the clearance of both bound and unbound ELP-GFP. Here, unbound ELP-GFP has a half-life of 1.7 h, as empirically determined, and bound ELP-GFP is cleared at the same rate as albumin (35 h for mice) (35). Importantly, the half-life of the protein is determined by three parameters in this model: 1) the half-life of unbound protein, 2) the half-life of serum albumin, and 3) the binding affinity between the protein and albumin. By simulating the kinetics over time, we were able to calculate the overall clearance rate, and predictions made by the model were in good agreement with the empirical measurements for KD and half-life (Fig. 3B and SI Appendix, Fig. S11). This suggests predictive capability for the half-life based on empirically determined KD values, or the model can provide a target KD based on the desired half-life. Together, our results confirm that titrating the number of FAs allows predictable tuning of the protein half-life by modifying the binding affinity to albumin.

To evaluate the biocompatibility of the ELP-GFP constructs, we assessed the biodistribution and inflammatory response in mouse. ELP(0FA)-GFP and ELP(10FA)-GFP labeled with Alexa Fluor 647 dye were administered intravenously, and after 3 or 48 h, the brain, lungs, heart, spleen, liver, kidneys, and blood were collected and imaged for far-red fluorescence (Fig. 3 C and D and SI Appendix, Fig. S12). In the case of ELP(0FA)-GFP, most of the reporter had cleared from the blood after 3 h, and a strong signal was observed in the kidney, whereas ELP(10FA)-GFP was clearly observed in the blood, and to a lesser extent in the kidney. For both samples, a small increase (20 to 30%) in signal was observed in the liver. After 48 h, the Alexa Fluor 647 signal was only observed in the blood of ELP(10FA)-GFPinjected mice, whereas the intensity in all other organs had returned to the basal level seen in the PBS injection control. These results are consistent with the rapid clearance of ELP(0FA)-GFP, which is likely to occur mostly through excretion from the kidneys. The blood from each of these conditions was further analyzed for signs of inflammation. We did not detect any elevation of proinflammatory cytokine levels after injection of ELP-GFP constructs compared to PBS injection, whereas injection with lipopolysaccharide (LPS) as positive control gave a clear inflammatory response at both 3 and 48 h (Fig. 3E). Together, these results show that FA conjugation enables half-life extension without long-term accumulation in organs, or eliciting an inflammatory response after intravenous injection.

Considering applications of this technology for peptide and protein drug delivery in humans, we evaluated if the use of multiple FAs per protein conveyed similar increases in binding affinity to HSA. We observed KD values of 19.3 3.9 M, 3.2 0.6 M, and 1.6 0.2 M for ELP-GFP constructs with 1, 5, and 10 FAs per protein, respectively (Table 1). These binding affinities closely mirror the values observed for MSA, suggesting that multisite lipidation of proteins could be a promising strategy to tailor protein half-life in humans.

In this study, we describe the design and production of sequenced-defined synthetic biopolymers conjugated with a programmable number of FAs to tailor the serum half-life of proteins. Specifically, the genetically encoded pAzF residues facilitate precise and programmable functionalization with FAs, which enables titration of the binding affinity to both MSA and HSA. We determined that the binding affinity to albumin was predictive of the serum half-life in mice, suggesting that the protein clearance can be tuned by controlling the number of conjugated FAs per protein. Notably, we measured serum half-lives of up to 33 h, which is 94% of the 35-h half-life of MSA. Importantly, with similar binding affinities for MSA and HSA, we hypothesize that the half-life of these same constructs will be higher in humans, given that HSA has a significantly longer half-life (19 d) (37). Furthermore, activity analysis of a trx fusion protein, trx-ELP(10FA), indicate at most a 50% activity loss (in the absence or presence of HSA), as compared with free trx. This compares favorably with other carriers reported to cause an 30- to 500-fold reduction in the activity of other peptides (3842). Although, as is true for any carrier, the effect of ELP fusion and FA conjugation on activity is expected to vary for each individual peptide, protein, or molecule, our proposed fusion partner is highly tunable, in ELP size, sequence, and FA number and position, which should enable future optimization of both pharmacokinetics and bioactivity of each drug candidate.

Lipidation is an appealing alternative to PEG, which has come under scrutiny due to concerns about immunogenicity (43, 44), and uncertainty about its degradation and clearance from the body (45). The use of FAs has clinical precedence, offers greater tunability than direct fusion to albumin, and has a well-established safety profile (46). However, the utility of current lipidation strategies is constrained by two factors. First, typically only moderate half-life extensions are achieved due to weak binding of pharmaceuticals with single FAs to albumin. Second, the ability to identify uniquely reactive residues without impacting bioactivity remains challenging with conventional labeling strategies. Our work addresses both limitations with a general methodology that enables tuning the half-life extension by titrating the number of FAs per protein, and the ability to design conjugation sites at monomeric precision enables facile screening of permissive residues to maintain bioactivity.

Unique to this work is the multisite and programmable placement of nsAAs to produce a biopolymer with tunable properties, enabled by sequence-defined insertion of multiple FAs per biopolymer for functionalization. The ELP can be placed at either of the termini, or pAzF residues can be positioned in the primary sequence of the protein, permitting the optimization of both bioactivity and half-life extension, which highlights the flexibility of this approach. Furthermore, bioorthogonal conjugation sites, such as pAzF residues, allow the attachment of a wide variety of chemical moieties to expand the palette of biological chemistry far beyond FAs at genetically encoded positions throughout the protein to enhance its functionality. This establishes a foundation for a new class of synthetic, sequence-defined biopolymers comprised of a combination of natural and synthetic monomers that unites the diversity of the chemical world with the monomeric precision of translation in biological systems. These biopolymers are uniquely enabled by recoded organisms with open coding channels dedicated to the template-directed incorporation of synthetic monomers. We envision that this work, together with further recoding efforts to open up additional coding channels dedicated for multiple distinct nsAAs (46), establishes the basis for novel and programmable biopolymers (47, 48) with broad utility in biological research, pharmaceuticals, materials science, and biotechnology.

All proteins were expressed in the GRO (E. coli C321.A, CP006698.1, GI:54981157) (4) containing a previously described OTS plasmid pAcFRS.1.t1 with a p15A origin of replication and chloramphenicol acetyltransferase selection marker (2). The ELP-GFP genes were expressed from a plasmid with colE1 origin of replication and a kanamycin resistance marker (SI Appendix, Fig. S13). Each ELP-GFP construct had 10 repetitive units of 15 amino acids (VPGAGVPGXGVPGGG), where residue X is either tyrosine or pAzF (SI Appendix, Tables S2 and S3). The gene for trx was chemically synthesized (IDT) and cloned using EcoRI and PpuMI restriction enzymes into an expression vector containing to create trx-ELP(10UAG) (SI Appendix, Table S4). The same trx gene was cloned into an empty-expression vector for expression of unfused trx.

All cultures were grown at 34C under shaking (220 rpm). Before expression, the expression strains were grown to confluence in 50 mL 2xYT media. This culture was used to inoculate 1 liter of 2xYT, containing 30 g/mL chloramphenicol, 20 g/mL kanamycin, 0.2% arabinose, and 1 mM pAzF. After 4 h, expression of ELP-GFP was induced, using a final concentration of 60 ng/mL anhydrotetracycline. Cells were harvested 24 h after inoculation by centrifugation at 4,000 g for 15 min at 4C.

The cell pellet was resuspended in PBS, pH 7.4, and lysed by sonication (12 cycles of 10-s sonication separated by 40-s intervals, 40% amplitude). Poly(ethyleneimine) was added to each lysed suspension to a final concentration of 1.25%, after which the soluble fraction was separated from the cell debris by 15 min of centrifugation at 4,000 g. ELP-GFP proteins were then purified by phase transition triggered by sodium citrate, followed by centrifugation at 15,000 g for 3 min to eliminate contaminant proteins that did not precipitate. Finally, native E. coli proteins were denatured at 75C, and removed by centrifugation. After three purification cycles, the ELP-GFP proteins to >95% purity as judged by Coomassie staining of SDS/PAGE gels.

When stated, pAF residues from ELP-GFP proteins were regenerated using ISAz, as previously described. In brief, diazotransfer reactions were performed for proteins at a concentration of 20 M using 200 equivalents of ISAz in 10 PBS (1.4 M NaCl, 0.1 M phosphate, 0.03 M KCl) pH 7.2 at room temperature. After 72 h, reactions were stopped by exchanging the buffer to PBS (1, pH7.4).

The ELP-GFP proteins were reacted with palmitic acid alkyne using copper(I)-catalyzed azide-alkyne Huisgen cycloaddition (click-chemistry). For this reaction, proteins were diluted to a final azide concentration of 30 M, 35% DMSO, 0.16 mM palmitic acid alkyne, 0.1 mM CuSO4 and 0.5 mM THPTA (premixed for 30 min), 5 mM aminoguanidine hydrochloride, and 5 mM sodium ascorbate. The click-chemistry reaction was incubated for 1 h at room temperature under constant, gentle mixing. After the reaction, the protein was buffer exchanged to PBS (pH 7.4) using Amicon filters (10 kDa molecular weight cutoff [MWCO]).

Proteins for biodistribution studies were further labeled at primary amines with an Alexa Fluor 647 succinimidyl ester. Proteins were diluted to 0.1 mg/mL, and mixed with 5 g/mL fluorophore in PBS for mild labeling. Excess dye was removed using Amicon filters (10 kDa MWCO).

Endotoxins were removed from all protein preparations used for animal experiments, using Pierce high-capacity endotoxin removal columns following the manufacturers protocol (Thermo Fisher Scientific, catalog # 88274). Prior to injection, endotoxin levels were confirmed to be under 0.1 endotoxin unit (EU) per injection using Gel-Clot LAL reagent with sensitivity of 0.06 EU/mL (Charles River, catalog #R12006).

The purity at the target residue was determined by quantitative MS. The ELP-GFP proteins were buffer exchanged and diluted to 15 M in digestion buffer (50 mM Tris, pH 8.0, and 0.5 mM CaCl2), and were digested with 1.5 M thermolysin (Promega) for 6 h at 80C. The resulting ELP-peptides were quantified using standard curves based on synthetic peptides (SI Appendix, Fig. S2). High-resolution MS data were collected using an Agilent iFunnel 6550 quadrupole time-of-flight (TOF) MS with an electrospray ionization (ESI) source, coupled to an Agilent Infinity 1290 ultrahigh-performance liquid chromatography system with an Agilent Eclipse Plus C18 1.8 m, 4.6 50-mm column. Solvents used were (solvent A) water 0.1% formic acid and (solvent B) CH3CN 0.1% formic acid. Mass spectra were gathered using Dual Agilent Jet Stream ESI in positive mode. The mass range was set from 110 to 1,700 m/z with a scan speed of three scans per second. The capillary and nozzle voltages were set to 5,500 and 2,000 V, respectively. The source parameters were set with a gas temperature of 280C and a flowrate of 11 liters/min, nebulizer at 40 psig, and sheath gas temperature at 350C at a flow of 11 liters/min. MS data were acquired with MassHunter Workstation Data Acquisition (version B.06.01, Agilent Technologies) and analyzed using MassHunterQualitative Analysis (version B.07.00, Agilent Technologies).

For MALDI-TOF analysis, 2 L of the protein samples were mixed in a ratio of 1:1:1 with 2% trifluoroacetic acid solution and then with the matrix solution (375 L of 20 mg/mL solution of 2,5-DHAP [2,5-dihydroxy acetophenone] in ethanol and 125 L of 18 mg/mL of aqueous DAC [diammonium hydrogen citrate solution]) by pipetting, until crystallization of the mixture. Then 0.5 L of the protein sample was loaded on MALDI steel target plate and analyzed after solvent evaporation.

MALDI-TOF MS spectra were acquired using an MALDI-TOF/TOF autoflex speed mass spectrometer (Bruker Daltonik), equipped with a smartbeam-II solid-state laser (modified Nd:YAG laser, = 355 nm), at the Ilse Katz Institute for Nanoscale Science and Technology (Ben-Gurion University of Negev, Beer-Sheva, Israel). The instrument was operated in positive ion, linear mode within a mass range from m/z 10 kDa to 50 kDa. Laser fluence were optimized for each sample. The laser was fired at a frequency of 1 kHz and spectra were accumulated in multiples of 500 laser shots, with 1,500 shots in total. Calibration was performed using protein calibration standard from Bruker. Spectrum analysis was performed by the Flexanalysis software.

Protein solubility and self-assembly was analyzed using a Zetasizer Nano ZS (Malvern Pananalytical). For each sample, 11 to 15 acquisitions (determined automatically by the instrument) were obtained at 25C for 10 M protein solutions in PBS. Three separately prepared samples were analyzed, and the analysis for each sample was repeated three times. Populations comprising less than 1% of the total mass (by volume) were excluded from the analysis.

Binding assays were performed on a Biacore T200 instrument. HSA (Sigma, catalog #A3782) or albumin from mouse serum (Sigma, catalog #A3139) were immobilized by amine coupling to research grade CM5 chip (GE Healthcare, catalog #BR100530) from 20 g/mL solutions in 10 mM acetate pH 5.0. High-density surfaces were created ranging from 1,300 to 12,800 RUs to minimize nonspecific binding of ELP-GFP derivatives. Binding was measured with 60-s association phase and 600-s dissociation phase with either no regeneration, or surfaces were regenerated with two 30-s pulses of 50 mM NaOH. ELP-GFP derivatives were injected in duplicates from twofold dilution series with at least six different concentrations ranging from 0.28 to 60 M (depending on the polymer and its expected Kd); PBS was used as running buffer. Data were doubly referenced against the signal collected on the reference cell and responses generated on the active cells during buffer injections. Data were analyzed using Evaluation software and fit into a steady-state affinity binding model. Each reported affinity is an average from four to eight independent measurements.

The activity of recombinant trx and trx fusion proteins were determined using the Proteostat thioredoxin-1 activity assay (Enzo). Trx catalyzed reduction of insulin and consequent aggregation of insulin in the presence of dithiothreitol (DTT) was monitored by a fluorescent dye. Trx activity was determined using a standard curve using a concentration range of bacterial trx, and the activity of samples were determined to be within the linear range of the assay. Fluorescence emission was monitored using a Biotek spectrophotometric plate reader.

All experiments were performed in C57BL/6J mice in accordance with the guidelines of the Animal Care and Use Committee of Yale University. Recommendations from the Guide for the Care and Use of Laboratory Animals (49) were followed during these experiments.

The half-lives of ELP-GFP constructs were calculated from concentrations measured from blood samples collected over the course of a week. The experiments were initiated by injecting 120 L of 10 M ELP-GFP intravenously or subcutaneously. At indicated times, 2 L blood was collected from a tail puncture, and diluted 1:25 in heparin tubes. The blood sample was vortexed briefly and cells were pelleted by centrifugation (2 min at 14,000 g). The soluble fraction was collected and frozen at 20C until analysis. ELP-GFP concentrations of the samples were determined using a GFP ELISA Kit (Abcam, catalog #ab171581). The samples were diluted in PBS as needed, to ensure that the concentration fell within the quantifiable range of the standard curve.

To study the immunogenicity and biodistribution of ELP-GFP, 120 L of 10 M Alexa Fluor 647 labeled constructs were injected, and blood and organs were collected at indicated times. As positive control for an inflammatory response, 100 g LPS was injected, and an injection of PBS was performed as negative control. Organs were imaged using Amersham Imager 600 RGB, and signal visualization and quantification were performed with FIJI (https://imagej.net/software/fiji/). For cytokine quantification, blood was allowed to coagulate, and serum was collected. Cytokines were quantified from the serum samples using the BD CBA Mouse Inflammation Kit (Fisher Scientific, catalog #BD 552364).

Data supporting the findings of this work are available within the paper and its supporting information files. The strains and plasmid (sequences) have been deposited in GenBank or Addgene: Genetically recoded organism C321.deltaA (RRID: Addgene_48998), aminoacyl tRNA synthetase (RRID: Addgene_73545), ELP-GFP reporter (Genbank: KT996142). All other study data are included in the article and/or SI Appendix.

We thank Jesse Rinehart, Michael Grome, and members of the F.J.I. laboratory for discussions and feedback on the manuscript; Terence Wu (Yale West Campus Analytical Core) for technical support; and Dr. Mark Karpasas from the Ilse Katz Institute for Nanoscale Science & Technology at Ben-Gurion University for professional help with the intact mass spectrometry experiments. We acknowledge support from the Gruber Foundation (K.V.), NIH Grant K99EB019501 (to M.A.), the Dahlia Greidinger Anti Cancer Fund (M.A.), Ben-Gurion University (M.A.), NIH Grant R01GM117230 (to F.J.I.), NSF Grant MCB-1714860 (to F.J.I.), the Arnold and Mabel Beckman Foundation (F.J.I.), DuPont Inc. (F.J.I.), and Yale University (F.J.I.) for funding.

Author contributions: K.V., P.A.-G., D.H., W.M.S., M.A., and F.J.I. designed research; K.V., P.A.-G., M.K., D.H., A.G., F.Y., E.F.-S., and M.A. performed research; K.V., P.A.-G., D.H., E.F.-S., and M.A. contributed new reagents/analytic tools; K.V., P.A.-G., M.K., D.H., E.F.-S., W.M.S., M.A., and F.J.I. analyzed data; and K.V., W.M.S., M.A., and F.J.I. wrote the paper.

Competing interest statement: K.V., P.A.-G., M.A., and F.J.I. have filed patents describing this work. F.J.I. is a cofounder of Pearl Bio, Inc.

This article is a PNAS Direct Submission.

This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.2103099119/-/DCSupplemental.

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Bill extending life of VISIT FLORIDA breezes through House tourism committee – Florida Politics

A bill to extend the sunset date of VISIT FLORIDA, the state-funded nonprofit tourism marketing corporation, breezed through the House Tourism, Infrastructure & Energy Subcommittee Tuesday.

VISIT FLORIDA would cease to exist after Oct. 1, 2023, under current law. The bill, HB 489, sponsored by St. Pete Beach Republican Rep. Linda Chaney, would extend its scheduled repeal date to Oct. 1, 2028.

Chaney said VISIT FLORIDA uses a targeted approach to attract visitors into Florida.

They run their ads and they know where the consumers are that theyre going to convince to come to the state of Florida, she said. By giving them an extended horizon, theyre going to be able to do that more cost effectively. If you are somebody whos engaging a vendor and they think you may only be a customer for a year, youre not going to get the same rate as somebody whos got a longer horizon.

The bill received some pushback. Critics have accused the organization of being a form of corporate welfare and a misuse of taxpayer money.

However well intentioned some government assistance may be, the fact remains that the practice of states picking winners and losers benefits a favorite few and leaves everyone else to pay the price, said Phillip Suderman, policy director for Americans for Prosperity. Too often the winners are special interests who lobby for special favors while those who bear the cost are everyday citizens and small businesses who have to pick up the tab.

Suderman was joined by other members of Americans for Prosperity in Tuesdays meeting. They, in turn, were joined in opposition by a few small business owners. And Rep. Rick Roth said some of the points being brought up were absolutely valid. But those issues, he said, were already addressed about five years ago.

Those things were taken care of by amending Enterprise Florida and VISIT FLORIDA because of people on the board of Enterprise Florida, Roth said. These arguments were targeted mainly at Enterprise Florida because people on the board were getting benefits. So they were picking winners and losers, but thats all been changed.

About five years ago, VISIT FLORIDA saw some massive housecleaning. The organization had been criticized by both Democrats and Republicans for misspending, ineffectiveness, sketchy bonuses and a lack of accountability. The agency was put on a short leash and only given year-by-year renewals. But last year, on the heels of the COVID-19 pandemic virtually shutting down tourism in Florida, the agency reported major returns.

According to data released by Floridas Office of Economic & Demographic Research, for every $1 of taxpayer money spent on the program, it returned $3.27. And the latest projections from the state show an average of $65.4 million invested into the program over the five-year extension. VISIT FLORIDA said it helped bring a record 131.42 million visitors to Florida in 2019 when it had a budget of around $80 million. And while Rep. Randy Fine pointed out about 99% of those people wouldve likely come either way, he also said far less than that extra 1% is needed for the organization to pay for itself.

What percentage of the visitors of Florida, if they come, make that $80 million worth it? Fine asked. It turns out that if less than one-quarter of 1% of the visitors to the state of Florida wouldnt have come anyway, if one out of every 400 people who came to the state only came because we offered the program, the program pays for itself. Thats the right way to think about a program like this.

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Canadian military’s new search and rescue aircraft wont be operating this summer – Ottawa Sun

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The military cant say at this point when C-295 fixed-wing aircraft will be available for missions.

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The Canadian militarys new fleet of search-and-rescue aircraft wont be operating this summer as originally planned because of delays caused by the pandemic and other technical issues.

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The military cant say at this point when C-295 fixed-wing aircraft will be available for missions.

National Defence spokesperson Dan Le Bouthillier said the plan was to have the new aircraft reach an initial operational capability in the summer. However this timeline is shifting to allow for aircraft certification and qualification which has been running longer than anticipated as a result of the volume and complexity of the work associated with developing the CC-295 capability, and the global COVID-19 pandemic situation, he explained in an email.

A revised schedule is currently being subjected to an extensive analysis that itself will take some time to complete because of its complexity, Le Bouthillier added.

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Sources told this newspaper there are also technical concerns regarding a centre-of-gravity issue due to the cabin configuration of the CC-295, commonly referred to as C-295, as well as problems involving search-and-rescue technicians parachuting from the planes back ramp.

Were aware of the parachute and gravity issues, which is why we conduct testing and trials, Le Bouthillier confirmed. In short, these are problems that will be addressed as we work towards ensuring the fleet is safe for operational use.

Canada announced in December 2016 that it was purchasing 16 C-295 aircraft for fixed-wing search and rescue as part of a $2.2-billion project. Initial operational capability for the new fleet was supposed to be mid-2021. That was later revised to summer 2022.

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Le Bouthillier said there are currently four C-295 aircraft in Canada and five at the manufacturers facility in Spain. Aircraft qualification work was ongoing in both locations, he added.

Earlier this month, the Royal Canadian Air Force removed from service its aging Buffalo search-and-rescue planes. Until the C-295s become operational, Hercules aircraft from Winnipeg will be used for search-and-rescue response on the west coast. Some of those aircraft will be based out of Comox, B.C., during the high-tempo period in the summer and will hold response posture from their home base at 17 Wing Winnipeg during periods of low operational tempo, according to the RCAF. In addition, up to five Cormorant search-and-rescue helicopters will be available.

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But that type of interim solution is dangerous, says the United Fishermen and Allied Workers Union-Unifor, which represents those involved in the west coast fishing industry.

We are working all-year-round, union president James Lawson said. Emergencies just dont happen during the busy season.

Lawson noted that fixed-wing search and rescue aircraft were essential to any response to an emergency and were able to cover large search areas. If a Hercules has to travel from Winnipeg to get to the west coast rescue location, that puts those seeking help at risk, he added.

Lawson said the union would raise the issue with members of Parliament and hope they respond. It doesnt seem right to begin with that we have to ask MPs to make sure were treated properly, he said. We shouldnt have to argue for safety.

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The Hercules aircraft, which are already widely used in a search-and-rescue role, are capable of responding quickly to emergencies such as ships in distress in challenging weather, the RCAF says. They can be used to parachute search-and-rescue technicians, emergency gear and food, life rafts, bailing pumps, arctic survival gear and illumination flares to support rescue missions.

The interim capability involving the Hercules will be in place until the new C-295 fleet is fully operational. That could happen in 2024 or 2025.

The RCAF says it had no choice but to remove the Buffalo aircraft from service. The fleet underwent a life extension program, which ensured the planes were able to safely and effectively support search-and-rescue operations up to Jan. 15, 2022. With the life extension program now concluded, the Buffalo was no longer able to support operations, the RCAF noted.

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Lesson of the Day:In a First, Man Receives a Heart From a Genetically Altered Pig – The New York Times

Lesson Overview

Featured Article: In a First, Man Receives a Heart From a Genetically Altered Pig by Roni Caryn Rabin

On Jan. 7, doctors in Maryland successfully transplanted a pigs heart into a human. The breakthrough may lead one day to new supplies of animal organs for transplant into human patients, Roni Caryn Rabin writes.

In this lesson, you will learn about the groundbreaking procedure and consider its place within the greatest medical advancements in history. In Going Further activities, we invite you to research other recent medical breakthroughs and to speculate on how far the field of medicine might extend life.

What, in your opinion, are the greatest medical achievements of all time?

With a partner or small group, brainstorm medical innovations and advances throughout history, such as aspirin, Band-Aids, X-rays, contraceptives and the recent coronavirus vaccine. Which do you think have made the biggest impact, whether by reducing pain, suffering and disease; giving comfort; or extending human life?

Then, choose one advancement from your list and make a one-minute case to the rest of the class for why it could be the single greatest medical achievement of all time.

Read the article, then answer the following questions:

1. Why was an eight-hour surgery in Baltimore on Jan. 7 global news? In your own words, describe what happened and why you believe it is making headlines around the world.

2. Understand the importance of organ replacement by finding some numbers in this article: How many Americans received a transplanted organ last year? How many people received human donor hearts last year? How many people are waiting for kidneys and other organs? And how many people on lists waiting for organs die each day? Taken together, what do these numbers tell you about the need for organ replacements in this country?

3. Who is David Bennett Sr. and why did he decide to gamble on the experimental treatment? Would you ever consider participating in a risky and experimental trial like Mr. Bennett did?

4. The article states that xenotransplantation, the process of grafting or transplanting organs or tissues from animals to humans, has a long history. Which examples from the article did you find most fascinating or significant?

5. Why a pig heart? What advantages do pigs offer over other animals for organ procurements?

6. Dr. David Klassen, the chief medical officer of the United Network for Organ Sharing and a transplant physician, called the news of the successful transplant a watershed event. Do you agree? How significant do you think it is? Return to your list from the warm up. Where would you place this pioneering surgery, or organ transplants more generally, on your list of greatest medical achievements?

7. Do you think that the use of animal organs for human transplants will become commonplace in the next 10 or 20 years? Should they? What ethical questions and concerns, if any, does the article raise for you?

Option 1: Learn more about other medical advances.

A mechanical womb to grow mouse embryos. A drug that brings drastic weight loss to patients with obesity. An unexpected key to understanding hair loss. These are just a few of the recent medical innovations and breakthroughs covered by The Times.

Choose one of the articles below or search for one that grabs your interest on the Timess Health topic page. Then, write or discuss with a partner: What is your reaction to the article? What was the most fascinating, surprising, provocative or memorable thing you learned? What questions do you still have about the scientific breakthrough you read about?

Option 2: Share your thoughts and opinions: How long can medical advancements extend life?

Last century, the average human life expectancy doubled. Medical and social advances such as the development of antibiotics and vaccines reduced childhood deaths, mitigated diseases of old age and vastly prolonged life. In Can We Live to 200?, Nicholas St. Fleur, Chloe Williams and Charlie Wood presented 43 advances that could radically extend life spans over the next 100 years. Look at the interactive timeline, then respond to the following prompts:

Which scientific advancements and breakthroughs in the article do you most look forward to? Which do you think will most likely come to fruition?

By 2100, how long might people be able to live? Do you think humans will reach the ages of 130, 150 or even 200?

Does the possibility of radical life extension intrigue, surprise, excite or even scare you? Would you want to live to 200? How long would you want to live, if you could choose your life span?

If you are interested in joining a conversation with other students, share your thoughts in our related Student Opinion prompt.

Additional Teaching and Learning Opportunities:

Learn more about the science behind the story: Read Heres How Scientists Pulled Off the First Pig-to-Human Heart Transplant from Science.org, which details how the effort involved genetic engineering, an experimental drug and cocaine. How did the article add to or change your understanding of the first successful transplant of a pigs heart into a human? What was most interesting or surprising? What questions do you still have?

Explore bioethical issues further: The Times later reported that Mr. Bennett Sr. had a criminal record stemming from an assault 34 years ago in which he repeatedly stabbed a young man, leaving him paralyzed. The victims brother and people on social media expressed outrage and questioned the choice to select Mr. Bennett as the recipient of the pioneering transplant procedure. However, Karen J. Maschke, a research scholar at the Hastings Center and the editor of the journal Ethics & Human Research, said, Theres a longstanding standard in medical ethics that physicians dont pick and choose who they treat. Read the article and give your reaction: How should we decide who receives a lifesaving treatment? Should a patients history affect the decision? Why or why not?

Want more Lessons of the Day? You can find them all here.

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Lesson of the Day:In a First, Man Receives a Heart From a Genetically Altered Pig - The New York Times

Fierce JPM Week: AstraZeneca cancer R&D head marks Imfinzi as ‘core backbone’ of oncology strategy with the right comboincluding treme – FiercePharma

In the increasingly crowded cancer immunotherapy field, AstraZenecas Imfinzi has mostly been used in stage III non-small cell lung cancer. But with the right combination strategy, the PD-L1 inhibitor has promise in other tumor types, AZs oncology R&D head said.

Imfinzi is a core backbone component of AZs overall strategy for oncology, Susan Galbraith, Ph.D., AZs executive vice president of oncology R&D, said during the Fierce JPM Week virtual conference. The drug just hit gold-standard life extension goals in two phase 3 trials when used alongside chemo in newly diagnosed biliary tract cancer and as part of a cocktail with AZs own CTLA4 inhibitor tremelimumab in first-line liver cancer.

Those trial wins fit AZs philosophy of developing treatments that can improve long-term survival and cure more patients by targeting earlier lines of treatment. But that ambition requires different components and mechanisms, such as the approach with Imfinzi and treme, Galbraith said.

In the first-line liver cancer trial dubbed Himalaya, the Imfinzi-treme pairing slashed the risk of death by 22% over Bayers aging standard-of-care Nexavar. That result marked a rare win for treme after multiple failures in non-small cell lung cancer (NSCLC), head and neck cancer and bladder cancer.

By incorporating lessons from previous trials, AZ's scientists homed in on a regimen called Stride, Galbraith said, which involves a single high dose of treme given in tandem with Imfinzi at the beginning of treatment, followed by Imfinzi alone.

That single punch of CTLA4 inhibition functions as priming the immune system. Previous research has found that with the proper priming, the bodys immune response against cancer can be very long-lasting, delivering a durability effect with an improvement particularly in the tail of the survival curve, Galbraith explained.

In the Himalaya trial, 40.5% of patients on the Stride regimen were still alive after two years, compared with 39.6% for the Imfinzi monotherapy group and 32.6% in the Nexavar arm. At three years, the survival rate stood at 30.7% for Stride, while Imfinzi monotherapy dropped faster to 24.7% and Nexavar to 20.2%.

The dose and schedule refinement, particularly with the Stride regimen, really gives us some insight into how we can improve the therapeutic index, Galbraith said.

Separately, by posting a 20% reduction in the risk of death previously untreated unresectable biliary tract cancer, Imfinzis chemo combo was the first immunotherapy regimen to improve survival in those patients.

RELATED:AstraZeneca climbs a Himalaya to find ill-fated treme a place alongside Imfinzi in liver cancer

Meanwhile, following its approach to target earlier lines of treatment with combos, AZ is running the phase 3 Emerald-1 trial to see whether adding Imfinzior Imfinzi and Roches VEGF inhibitor Avastinon top of transarterial chemoembolization could help patients with locoregional liver cancer. That data could read out later this year. A separate Emerald-2 trial is examining Imfinzi alone or the Imfinzi-Avastin pairing as a post-surgery adjuvant therapy in early liver cancer patients at high risk of recurrence.

Roche won an FDA go-ahead in 2020 for its PD-L1 inhibitor Tecentriq alongside Avastin in newly diagnosed liver cancer. Given that success, Galbraith said shes optimistic about the two Emerald trials.

Beyond treme, AZ recently turned in positive phase 2 results for Imfinzis combination with oleclumab, an anti-CD73 antibody, or monalizumab, an anti-NKG2A antibody, over solo Imfinzi in stage III non-small cell lung cancer. Now AZ is moving both new therapies into phase 3 testing.

AZ gained full rights to monalizumab from a collaboration with Innate Pharma originally signed in 2015. Last week, AZ paid Scorpion Therapeutics $75 million upfront to develop drugs against transcription factors, which have historically been hard to drug.

RELATED:AstraZeneca names Galbraith as Baselga successor in cancer R&D

The British pharma is also known for two billion-dollar licensing deals with Daiichi Sankyo featuring antibody-drug conjugates Enhertu and datopotamab deruxtecan. BTK inhibitor Calquence also came to AZ while it was in late-stage development at Acerta Pharma.

While AZ has a productive internal R&D engine, the company doesn't have a monopoly on all innovation, Galbraith said. Moving forward, AZ is very open to deals across the spectrum of drug development stages for products that can deliver value, she said.

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Fierce JPM Week: AstraZeneca cancer R&D head marks Imfinzi as 'core backbone' of oncology strategy with the right comboincluding treme - FiercePharma