Fighting cancer and Ebola with nanoparticles

Story highlights Scientists in the U.S. are applying nanotechnology research to the battle against cancer and Ebola Man-made nanostructures would attach themselves to viruses or cancerous cells, nullifying them Nanostructured surfaces are already in use for medical conditions and implants, reducing the risk of infection

From targeted remedies such as monoclonal antibodies to surgery, cancer has still managed to elude a treatment that discretely and separately attacks it alone.

Nanotechnologies, however - the manipulation of matter at a molecular and even atomic scale to penetrate living cells -- are holding out the promise of opening a new front against deadly conditions from cancer to Ebola.

According to Dr Thomas Webster, the chair of chemical engineering at Northeastern University in Boston, research into medical nanotechnology is gaining pace and the medical establishment is starting to sit up and pay attention.

At the core of the technology is the ability to attach drugs, and in some cases metals and minerals, to nanoparticles that would then bind themselves to life threatening cancer cells or viruses.

In one study, Dr Webster's team is developing methods to attach gold nanoparticles to cancer cells.

Infrared light would then heat up the nanoparticles, killing the cancer cells with heat but leaving the healthy cells alive to do their job.

"This technology has been studied for the better part of a decade, but we're looking at ways of making it better," Dr Webster told CNN. "One that we've created in the lab we've called 'nanostars.'

"A star shape has a lot more surface area, so they can kill cancer cells faster than a nanosphere because they heat up faster.

"Even if it's carrying a drug, a star has a lot more surface area on which to attach it -- it's got a different morphology."

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Fighting cancer and Ebola with nanoparticles

Fluctuation X-ray scattering

IMAGE:In this image, density slices through the center a virus. view more

Credit: Malmerberg et al.

In biology, materials science and the energy sciences, structural information provides important insights into the understanding of matter. The link between a structure and its properties can suggest new avenues for designed improvements of synthetic materials or provide new fundamental insights in biology and medicine at the molecular level.

During standard X-ray solution scattering experiments, molecules tumble around during X-ray exposures, resulting in an angularly isotropic diffraction pattern because of the full orientational averaging of the molecules that scatter X-rays. When X-ray snapshots are collected at timescales shorter than a few nano-seconds, such that molecules are virtually frozen in space and time during the scattering experiment, X-ray diffraction patterns are obtained that are no longer angularly isotropic. These measurements, called fluctuation X-ray scattering, are typically performed on an X-ray free electron laser or on a ultra-bright synchrotron and can provide fundamental insights into the structure of biological molecules, engineered nanoparticles or energy-related mesoscopic materials not attainable via standard scattering methods.

A group of scientists from the Lawrence Berkeley National Laboratory [Malmerberg et al., (2015), IUCrJ, 2, doi:10.1107/S2052252515002535] recently presented an intuitive view of the nature of fluctuation X-ray scattering data and their properties. The scientists have shown that fluctuation scattering is a natural extension of traditional small-angle X-ray scattering and that a number of fundamental operational properties translate from small- and wide-angle X-ray scattering into fluctuation scattering. The authors also show that even with a fairly limited fluctuation scattering dataset, the amount of recoverable structural detail is greatly increased as compared to what can be obtained from standard SAXS/WAXS experiments. Given that the high-quality structural models can be obtained from fluctuation scattering data and the ever-increasing availability of X-ray sources at which these experiments can be performed, the researchers expect that fluctuation scattering experiments will become routine in the future.

"Although fluctuation scattering experiments are not standard or routine at the moment, this work enables us to assess the quality of experimental data and allows us validate our experimental protocols and data reduction routines" Peter Zwart says.

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Fluctuation X-ray scattering

Nanoscale worms provide new route to nano-necklace structures

IMAGE:This schematic shows the synthesis of organic-inorganic shish kebab-like nanohybrids composed of periodic nanodisk-like kebabs. view more

Credit: Credit: Zhiqun Lin

Researchers have developed a novel technique for crafting nanometer-scale necklaces based on tiny star-like structures threaded onto a polymeric backbone. The technique could provide a new way to produce hybrid organic-inorganic shish kebab structures from semiconducting, magnetic, ferroelectric and other materials that may afford useful nanoscale properties.

The researchers have so far made nano-necklaces with up to 55 nanodisks. The template-based process grows amphiphilic worm-like diblock copolymers through a living polymerization technique in which the polymeric structures serve as nanoreactors that form laterally connecting nanocrystalline structures based on a variety of precursor materials. The nanodisks average about ten nanometers in diameter and four nanometers in thickness, and are about two nanometers apart.

"Our goal was to develop an unconventional, yet robust, strategy for making a large variety of organic-inorganic hybrid shish kebabs," said Zhiqun Lin, a professor in the School of Materials Science and Engineering at the Georgia Institute of Technology. "This is a general technique for making these unusual structures. Now that we have demonstrated it, we believe there is a nearly endless list of materials we can use to craft these nano-necklaces."

The research was supported by the Air Force Office of Scientific Research and the National Science Foundation. The results were scheduled to be published on March 27 in the journal Science Advances, published by the American Association for the Advancement of Science (AAAS).

The one-dimensional nano-necklaces could have optical, electronic, optoelectronic, sensing and magnetic applications. The researchers have so far produced structures from cadmium selenide (CdSe), barium titanate (BaTiO3) and iron oxide (Fe3O4), but believe many other materials - including gold--could also be used.

The technique begins with formation of inclusion complexes made of alpha-cyclodextrins, cyclic oligosaccharides composed of six glucose units. The alpha-cyclodextrins, which are hollow in the center, thread themselves onto a polyethylene glycol (PEG) chain in an established self-assembly process. The polymer backbone on which the alpha-cyclodextrins are threaded is capped by a larger stoppering agent to retain the tiny structures.

Each alpha-cyclodextrin has 18 hydroxyl (OH) groups that can be converted into bromine (Br) groups through an esterification process. Diblock polymer "nanoworm" structures are then grown from these bromine groups in solution. Formed from poly(acrylic acid)-block polystyrene (PAA-b-PS), the worm-like diblock copolymers are made up of inner poly(acrylic acid) (PAA) blocks that are hydrophilic, and outer polystyrene (PS) blocks that are hydrophobic. Because so many diblocks grow on each alpha-cyclodextrin, their crowding stretches the polymer backbone.

Finally, metallic ion precursors are preferentially incorporated into the space occupied by inner PAA blocks of worm-like diblock copolymer nanoreactors, forming crystals. These crystals connect the once separate structures, creating the nano-necklaces - which resemble tiny centipedes.

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Nanoscale worms provide new route to nano-necklace structures

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