Evolutionary biologist Stephen Jay Gould once pondered what would happen if the cassette tape of life were rewound and played again. Synthetic biologists have tested one aspect of this notion by engineering chromosomes from scratch, sticking them into yeast and seeing whether the modified organisms can still function normally.
They do, according to seven papers published today inSciencethat describe the creation, testing and refining of five redesigned yeast chromosomes. Together witha sixth previously synthesized chromosome, they represent more than one-third of the genome of the bakers yeastSaccharomycescerevisiae.An international consortium of more than 200 researchers that created the chromosomes expects to complete a fully synthetic yeast genome by the end of the year.
The work the team has already done could help to optimizethe creation of microbes to pump out alcohol, drugs, fragrances and fuel. And it serves as a guide for future research on how genomes evolve and function.
The amazing thing here is that they are figuring out how to tweak the genomenot just synthesize itthrough a design-build-test-learn cycle, says Jack Newman, co-founder of Amyris Biotechnologies in Emeryville, California. The approach is similar to one that computer scientists might take when trying to understand a computer code written a decade ago, he adds, although the task is much harder with genomes that have undergone millions of years of evolution. Yeast originated more than 50 million years ago, when theSaccharomyceslineage branched off from other fungi.
In 2010, geneticist Craig Venter and his team revealedthe first synthetic genome, a stripped-down version of the genetic code from a bacterial parasite,Mycoplasma mycoides. Four years later, a team led by Jef Boeke, a yeast geneticist at New York University Langone Medical Center in New York City, synthesizeda chromosome from yeast, a more complex organism that is classified as a eukaryotea group that also includes plants, worms and people.
Venters goal was to realizethe smallest genome needed to sustain life, but Boeke sought to explore fundamental questions about evolution, such as whether yeasts could have evolved through alternate routes. He turned the query into a hypothesis testable with synthetic biology: how much can you change a genome and still have a working organism?
To look for an answer, Boeke assigned each ofS. cerevisiaes 16 chromosomes to teams of collaborators, spread across the United States, United Kingdom, China, Singapore and Australia. Each was to create a chromosome that was stable yet evolvable, and would keep yeast functioning as usual.
The teams used computer programs to design the codes of their respective chromosomes. They omitted some sequences found in naturally occurring yeast chromosomes, such as repetitive parts of the genome, in hopes of increasing the stability of the synthetic versions. And they endowed their creations with a mechanism that mimics the random variation that drives evolution. When this scrambling system is triggered, it can shuffle, duplicate and delete genes at random.
A team led by researchers at the Pasteur Institute in Paris documented dramatic structural changes in the nucleus of the synthetic yeasteven as it continued to thrive, making proteins and reproducing. It seems like we can really kind of torture the genome in complicated ways and frequently the yeast shrugs its shoulders and grows like normal, Boeke says.
Some teams in the consortium invented techniques to rapidly identify errors in synthetic chromosomes. Another group, led researchers at Tianjin University in China, optimized techniques to remove bugs in the genetic sequences of the chromosomes, in one instance by using the gene-editing tool CRISPRCas9.
Considering that they synthesized 536,024 base pairs in that chromosome and only used CRISPR to mess around with 45 of them is kind of refreshing, says George Church, a geneticist at Harvard University in Cambridge, Massachusetts. It makes you feel like maybe this is the next big thing.
Genome synthesis is unlikely to displace tools such as CRISPR, which allow scientists to add or subtract a limited number of genes in an organism, he says. But it may become the favoured method for applications that require complicated genetic changes. This includes engineering yeast and other microbes to produce fragrances and other materials; manufacturers that rely on such microbes could use synthetic genomes to make those organisms more resilient to harmful viruses, for example.
If you took those [microbe] strains offline and reprogrammed their code, then put them back in, the viruses would be so far out of touch they couldnt come back, Church says. It would be like going back to the Middle Ages and giving one country hydrogen bombs.
Several groups have launched efforts to synthesize genomes from species such as the bacteriumEscherichia coliand from people. Boeke is confident that his consortium will create a fully synthetic yeast genome by the years end. The team has already created several additional chromosomes, and is debugging and testing them.
The groups latest results will encourage others to dream big, Church says: Theyve been able to induce radical changes in the code, so it emboldens you to be even more radical.
This article is reproduced with permission and wasfirst publishedon March 9, 2017.
Read more:
Synthetic Yeast Chromosomes Help Probe Mysteries of Evolution - Scientific American
- History of Evolution | Internet Encyclopedia of Philosophy - December 9th, 2016 [December 9th, 2016]
- Evolution - Bulbapedia, the community-driven Pokmon encyclopedia - December 12th, 2016 [December 12th, 2016]
- What is Evolution - explanation and definitions - December 21st, 2016 [December 21st, 2016]
- Evolution (2001 film) - Wikipedia - January 28th, 2017 [January 28th, 2017]
- EvolutionM.net - Mitsubishi Lancer Evolution | Reviews, News ... - February 1st, 2017 [February 1st, 2017]
- YMCA evolution continues at lake - Gaston Gazette - February 7th, 2017 [February 7th, 2017]
- Ivanka Trump's Beauty Evolution, From 1998 to Today Watch - Us Weekly - February 7th, 2017 [February 7th, 2017]
- Lumpy, hairy, toe-like fossil could reveal the evolution of molluscs - The Guardian - February 7th, 2017 [February 7th, 2017]
- How Evolution Alters Biological Invasions - ScienceBlog.com (blog) - February 7th, 2017 [February 7th, 2017]
- Cultural evolution and the mutilation of women - The Economist - February 7th, 2017 [February 7th, 2017]
- Late-night hosts on the evolution of Trump: 'Dickish to dictatorish' - The Guardian - February 7th, 2017 [February 7th, 2017]
- Gold's Gym Regina rebrands to become Evolution Fitness - Regina Leader-Post - February 7th, 2017 [February 7th, 2017]
- Incremental Versus Radical Innovation: A Response to Josh Swamidass on Evolution and Cancer - Discovery Institute - February 7th, 2017 [February 7th, 2017]
- Blockchain: Investment (R)Evolution For Developing Markets - Forbes - February 7th, 2017 [February 7th, 2017]
- See the Evolution of the Famed Porsche 911 in 7 Photos - WIRED - February 7th, 2017 [February 7th, 2017]
- Exhibition charts 500 years of evolution of robots - Phys.Org - February 7th, 2017 [February 7th, 2017]
- How evolution turned ordinary plants into ravenous meat-eaters - Wired.co.uk - February 7th, 2017 [February 7th, 2017]
- Are Evolution Fresh Drinks 'Poison'? - snopes.com - February 7th, 2017 [February 7th, 2017]
- Non-Chromosomal DNA Drives Tumor Evolution - The Scientist - February 8th, 2017 [February 8th, 2017]
- Chimpanzee feet allow scientists a new grasp on human foot evolution - Phys.Org - February 8th, 2017 [February 8th, 2017]
- 'Goldilocks' genes that tell the tale of human evolution hold clues to variety of diseases - Science Daily - February 8th, 2017 [February 8th, 2017]
- Pac-Man is Coming to 'The Sandbox Evolution' Next Week - Touch Arcade - February 9th, 2017 [February 9th, 2017]
- Chimpanzee feet allow scientists a new grasp on human foot ... - Science Daily - February 9th, 2017 [February 9th, 2017]
- Bacteria sleep, then rapidly evolve, to survive antibiotic treatments - Phys.Org - February 9th, 2017 [February 9th, 2017]
- Orangutan squeaks reveal language evolution, says study - BBC ... - BBC News - February 9th, 2017 [February 9th, 2017]
- Evolution gives rhyme its reason - Aurora News Register - February 9th, 2017 [February 9th, 2017]
- Deeper origin of gill evolution suggests 'active lifestyle' link in early vertebrates - Science Daily - February 9th, 2017 [February 9th, 2017]
- From Tara Palmer-Tomkinson to Cara Delevingne: the evolution of the It girl - The Guardian - February 10th, 2017 [February 10th, 2017]
- Banned TED Talk: Rupert Sheldrake The Science Delusion - Collective Evolution - February 10th, 2017 [February 10th, 2017]
- VOTD: Watch the Evolution of Keanu Reeves' Acting Career - /FILM - February 10th, 2017 [February 10th, 2017]
- Pokmon Go Eevee evolution: How to evolve Eevee into Vaporeon, Jolteon and Flareon with new names - Eurogamer.net - February 10th, 2017 [February 10th, 2017]
- Horse evolution bucks evolutionary theory - Science News - February 10th, 2017 [February 10th, 2017]
- Samsung's Chromebook Pro highlights the category's continued evolution - TechCrunch - February 10th, 2017 [February 10th, 2017]
- Scientists solve fish evolution mystery - Phys.Org - February 10th, 2017 [February 10th, 2017]
- Wildfire evolution forces Forest Service into new thinking - The Daily Progress - February 11th, 2017 [February 11th, 2017]
- How the horse can help us answer one of evolution's biggest questions - Raw Story - February 11th, 2017 [February 11th, 2017]
- A primer on Darwin Day: Some religious groups embrace 'Theistic evolution' - LancasterOnline - February 11th, 2017 [February 11th, 2017]
- Apple: Evolution of in-car audio tech moving at 'speed of sound ... - Times of India - February 11th, 2017 [February 11th, 2017]
- Mariska Hargitay's Evolution from '80s Glam to Law & Order: Special Victims Unit - TVOvermind - February 12th, 2017 [February 12th, 2017]
- Evolution of baseball from power to speed has left SBs behind ... - Chicago Sun-Times - February 12th, 2017 [February 12th, 2017]
- More order with less judgment: An optimal theory of the evolution of cooperation - Science Daily - February 12th, 2017 [February 12th, 2017]
- J. Albert C. Uy speaks on evolution, biodiversity in bellied flycatcher population - The College Reporter - February 12th, 2017 [February 12th, 2017]
- See the Evolution of Movie Magic With Every Oscar Winner for Visual Effects in History - Gizmodo - February 12th, 2017 [February 12th, 2017]
- Numerology: Here's What Your Name Says About You - Collective Evolution - February 13th, 2017 [February 13th, 2017]
- The Evolution of Valentine's Day - Inside Science News Service - February 13th, 2017 [February 13th, 2017]
- Why evolution may be tech billionaires' biggest enemy - The Week Magazine - February 13th, 2017 [February 13th, 2017]
- Community Viewpoint: Evolution, like gravity, is much more than theory it is a fact - Kdminer - February 13th, 2017 [February 13th, 2017]
- How the horse can help us answer one of evolution's biggest questions - Phys.Org - February 13th, 2017 [February 13th, 2017]
- How evolution alters biological invasions - Science Daily - February 13th, 2017 [February 13th, 2017]
- Cockeyed squid shines light on deep sea evolution - Christian Science Monitor - February 13th, 2017 [February 13th, 2017]
- Eye Evolution: A Closer Look - Discovery Institute - February 14th, 2017 [February 14th, 2017]
- Evolution always wins: University of Idaho video game uses mutating aliens to teach science concepts - The Spokesman-Review - February 14th, 2017 [February 14th, 2017]
- Geneticists track the evolution of parenting - Phys.Org - February 14th, 2017 [February 14th, 2017]
- How this cockeyed squid shines a light on deep sea evolution - Christian Science Monitor - February 14th, 2017 [February 14th, 2017]
- 4 Possible Roadmaps For macOS and iOS Evolution - The Mac Observer (blog) - February 15th, 2017 [February 15th, 2017]
- The Evolution of the Energy Capital of the World - Texas Monthly - February 15th, 2017 [February 15th, 2017]
- Humons presents an atypical dance evolution - Detroit Metro Times - February 15th, 2017 [February 15th, 2017]
- Pokemon Go Adds 80 Generation 2 Pokemon, New Evolution Items This Week - IGN - February 15th, 2017 [February 15th, 2017]
- Fossil discovery rewrites understanding of reproductive evolution ... - Science Daily - February 15th, 2017 [February 15th, 2017]
- 'X-Men: Evolution' Is the Gateway Drug of Comic Book Shows - Geek - February 16th, 2017 [February 16th, 2017]
- A cultural catch: Evolution of wooden halibut hooks carved by native ... - Science Daily - February 16th, 2017 [February 16th, 2017]
- Bremerton's Fitness Evolution now Planet Fitness - Kitsap Sun (blog) - February 16th, 2017 [February 16th, 2017]
- Eye Evolution: The Waiting Is the Hardest Part - Discovery Institute - February 16th, 2017 [February 16th, 2017]
- Evolution Of The Yeezy: 2009-2017 - HotNewHipHop - February 16th, 2017 [February 16th, 2017]
- Prebiotic evolution: Hairpins help each other out - Science Daily - February 16th, 2017 [February 16th, 2017]
- This 'Live Birth' Fossil Could Change Humanity's Understanding Of Evolution - Daily Caller - February 16th, 2017 [February 16th, 2017]
- Mysterious Ancient Stonehenge-Like Circles Found in Amazon Rainforest - Collective Evolution - February 16th, 2017 [February 16th, 2017]
- 'Pokemon Go': How to Evolve Poliwhirl Into Politoed - Heavy.com - February 17th, 2017 [February 17th, 2017]
- 'Pokemon Go': How to Evolve Slowpoke Into Slowbro or Slowking - Heavy.com - February 17th, 2017 [February 17th, 2017]
- 'Pokemon Go': How to Evolve Gloom Into Bellossom - Heavy.com - February 17th, 2017 [February 17th, 2017]
- Pokmon Go Dragon Scale - how to evolve Seadra into Kingdra and how to get the Dragon Scale - Eurogamer.net - February 17th, 2017 [February 17th, 2017]
- Pokmon Go Eevee evolution: How to evolve Eevee into Umbreon, Espeon, Vaporeon, Jolteon and Flareon with new ... - Eurogamer.net - February 17th, 2017 [February 17th, 2017]
- University of Pittsburgh guest speaker discloses evolution findings - UTA The Shorthorn - February 17th, 2017 [February 17th, 2017]
- 'Pokemon Go' Special Items: Drop Rates for Evolution Items & Berries at Pokestops - Heavy.com - February 17th, 2017 [February 17th, 2017]
- How Vedic Philosophy Influenced Nikola Tesla's Idea of 'Free Energy' - Collective Evolution - February 18th, 2017 [February 18th, 2017]
- Migration to America took long enough for evolution to happen on the way - Ars Technica - February 18th, 2017 [February 18th, 2017]
- How To Choose Your Eevee Evolution In 'Pokmon GO:' Umbreon And Espeon Edition - Forbes - February 18th, 2017 [February 18th, 2017]
- Evolution Items - IGN - February 18th, 2017 [February 18th, 2017]
- Congo River fish evolution shaped by intense rapids: Genomic study ... - Science Daily - February 18th, 2017 [February 18th, 2017]
- Pokmon Go - How to evolve, use Special Items, when to evolve or Power Up your Pokmon - Eurogamer.net - February 18th, 2017 [February 18th, 2017]