Limited Insurance Choices Frustrate Patients In California …

Dennie and Kathy Wright sift through a stack of medical bills at their home in Indian Valley, Calif. Pauline Bartolone for NPR hide caption

Dennie and Kathy Wright sift through a stack of medical bills at their home in Indian Valley, Calif.

Dennie Wright lives in Indian Valley, a tiny alpine community at the northern end of the Sierra, close to the border with Nevada.

Wright works as a meat cutter in a grocery store and lives in a modest home overlooking a green pasture. He also lives in one of the 250 ZIP codes where Blue Shield of California stopped selling individual policies in 2014. As his insurance agent explained it, Wright had only one choice of companies if he wanted to buy insurance on Covered California, the state's health insurance exchange. That lone option was Anthem Blue Cross, so Wright bought one of the Anthem policies.

"That was new to us, you know, Covered California," Wright says. "Anthem Blue Cross was the insurance carrier. Then of course, three months later, I have a heart attack."

More than once, he was flown across the state line to Reno for care. Wright and his wife, Kathy, now have piles of medical bills and insurance paperwork. Though Anthem Blue Cross covers emergency care out of state, it doesn't cover routine doctor care outside a patient's home state. But Wright says traveling from his home to doctors on the California side of the mountains is not as safe or as convenient as going to Reno.

He continues to see the Nevada doctors who put a defibrillator in his chest and saved his life. Anthem Blue Cross will pay some of the bills, but the Wrights still don't know if everything will be covered.

There are other insurance options for Wright, but not through Covered California. Although he didn't need a subsidy, he was left in the same position as people in his area who do need financial help to buy insurance. People with lower incomes can't readily take their business to a competitor, because the state exchange is the only place customers can use federal subsidies to help them buy health insurance. So for these people who are pinched financially, Anthem is the only option.

"I mean, you should have some choices, especially if you're going to have one that's not going to cover you in the places you choose to go," Wright says.

Last July, Covered California Executive Director Peter Lee offered a different impression of choices the marketplace would offer.

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For Qualcomm, health care, autos represent the next …

In an interview with CNET, the mobile-chip maker's president, Derek Aberle, discusses his company's strategy to grow in 2015, as well as its China troubles.

Qualcomm's booth at CES 2015, filled with robots, wearables, VR goggles, a car and plenty more. Sarah Tew/CNET

LAS VEGAS -- The brightly colored Qualcomm booth at this year's Consumer Electronics Show packed in a panoply of gadgets: There was a red-dragon robot, a blue connected car with Qualcomm's logo splashed across the hood and disembodied mannequin hands in glass cases wearing a range of smartwatches.

As thousands of people walked through the loud and sprawling Las Vegas Convention Center -- with Qualcomm's space right in the middle of the chaos -- Derek Aberle, the company's president, sat in a quiet meeting room tucked in the back of the expansive booth to discuss his company's strategies for 2015.

As the booth illustrated, Qualcomm -- already the world's biggest maker of mobile chips -- is hoping to use its wealth of research in mobile devices to become the company powering wearables, robots, cars, medical technologies and more.

Aberle at Qualcomm's CES 2015 press conference, where he discussed the company's plans to expand into a lot more areas this year. Josh Miller/CNET

"We look at it and say, OK, with a relatively small incremental investment and the right go-to-market," Aberle said of Qualcomm's push into new tech sectors, "we can capture a pretty big opportunity, just leveraging this massive investment we made because of the scale of the smartphone business."

Qualcomm's expansion effort comes as tech firms scramble to connect millions of objects to the Web -- an idea known as the Internet of Things -- creating vast new ground for chipmakers like Qualcomm to gain new business. While the Internet of Things area offers huge potential, its already invited plenty of competition from Qualcomm's rivals. For example, fellow chipmaker Intel -- whose booth was stationed right next to Qualcomm's on the convention floor -- constructed a booth just as broad and filled with all kinds of connected devices.

It's clear why these companies are so keen to stake a claim in this area: the market for Internet of Things is expected to grow to $3.04 trillion by 2020, according to market research firm IDC.

While Qualcomm tries to capture a piece of all these new markets, the maker of Snapdragon mobile chips has been distracted by an ongoing investigation by Chinese regulators. Officials there have spent more than a year looking into whether the chipmaker's lucrative licensing business runs afoul of the country's anti-monopoly laws. Aberle couldn't say when the investigation might be resolved, but he said it was unlikely any negative outcome out of China would -- as some analysts predict -- create a domino effect, with fines or new limitations spreading to more countries.

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For Qualcomm, health care, autos represent the next ...

Medicare Chief Steps Down, Ran Health Care Rollout – ABC News

Medicare's top administrator unexpectedly resigned Friday, becoming the latest casualty in the turmoil over the president's health care law, which is still struggling for acceptance even as millions benefit from expanded coverage.

Marilyn Tavenner's departure underscores the uncertainty overshadowing President Barack Obama's health care law nearly five years after its party-line passage by a then-Democratic-led Congress. The Supreme Court will hear a challenge to the legality of the law's financial subsidies this spring, and a new Republican Congress is preparing more repeal votes.

A former intensive care nurse with a businesslike approach to a divisive area of public policy, Tavenner told staff in an email that she's stepping down at the end of February with "sadness and mixed emotions." Her chief of staff is also leaving.

Tavenner, 63, survived the technology meltdown that initially paralyzed HealthCare.gov. She remained in place even as her boss, former Health and Human Services secretary Kathleen Sebelius, left office following signals of White House unhappiness.

But Tavenner was embarrassed last fall when she testified to Congress that 7.3 million people were fully enrolled for private coverage under the health law. That number turned out to be an over-count that exaggerated the total by about 400,000 people. The error, discovered by Republican congressional staff, was termed "unacceptable" by new HHS Secretary Sylvia M. Burwell.

Tavenner had a played a key role in the 2013 decision to go live with HealthCare.gov, signing a required cybersecurity clearance after technology professionals under her balked because testing was incomplete. The website later passed security tests and received full authority to operate.

In her farewell message, Tavenner termed the health law's online insurance markets "a success." But she also said her job, which involves oversight of Medicare and Medicaid as well, was a "huge and complex responsibility" and "we had many additional challenges put before us" because of Obama's health law. Roughly 1 in 3 Americans are covered by health insurance programs run by the Centers for Medicare and Medicaid Services.

Despite Tavenner's close association with "Obamacare," some senior Republicans in Congress said they were sorry to see her leave.

"She has proven herself to be a strong leader and a straight shooter who brought in much-needed private sector sensibility into the agency," Sen. Orrin Hatch, R-Utah, said in a statement. "I truly appreciate her service and wish her the very best in her next adventure."

But former House oversight chairman Darrell Issa, R-Calif., said, "Tavenner had to go." The over-count, discovered by his staff, "was a deplorable example of an agency trying to scam the American people," Issa said. The administration insists it was only a mistake, resulting from a double-count of people with dental coverage.

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Medicare Chief Steps Down, Ran Health Care Rollout - ABC News

Health care benefits in police contract could be budget buster

A revised contract that would provide health care benefits to retired Scranton police officers could be a future budget buster that would cripple the city financially for decades to come, a Times-Tribune analysis shows.

Mayor Bill Courtrights administration has touted the deal as a significant breakthrough that will save the city up to $4.5 million over the life of the contract. Those savings dont account for the possibility the city could be obligated to pay costly health care benefits for decades to come, however.

The debate centers on how many people will qualify for the benefits. The citys analysis is based on six people being eligible for the benefits, but that assumes the city will be able to remove the perk after the contract expires in 2021. If the city cannot, it would be obligated to pay the benefits to 99 current employees in the ensuing decades, the newspapers review found.

The mindset is the union will give it back. Thats just not the way it works, said Councilman Wayne Evans, one of three council members who voted Thursday to table a vote on the memorandum of understanding that would alter the current contract. Its very hard to get any kind of concession. Thats a very, very attractive benefit. ... Once its in there, thats a budget buster and something the city could not sustain.

The health care provision is among several enhanced benefits the city offered in exchange for concessions from police that Police Chief Carl Graziano estimated could save $4.5 million. The citys labor attorney, Edwin Abrahamsen, previously estimated the savings at $750,000 to $1 million a year. That analysis did not include all costs and revenue, however.

Mr. Grazianos savings include $1.3 million from eliminating a minimum manning clause, roughly $846,000 from other staffing changes and $2.5 million in increased revenue from parking violations that will result from having two civilians dedicated to writing tickets.

The analysis, which was provided to council on Thursday, did not include a deduction for the cost of the health care benefits. Mr. Abrahamsen on Friday said he estimated that cost would be $445,000 over a 10-year-period, which would start in 2020 and 2021 the first year anyone would qualify for the benefit.

That calculation is based on the assumption only two of the six officers who are eligible will actually retire, he said. Even if all six retire, the cost would be $1.3 million over 10 years.

Mr. Evans and councilmen Joe Wechsler,Bill Gaughan and Bob McGoff said that cost, if accurate, might be manageable. The question, they said, is whether the city can afford to gamble that it will be able to halt the health care benefits in the future.

The contract would provide the benefits to officers who were hired after Jan. 1, 1994 and retire with 25 years of service. The benefit would extend to those employees spouses. The city requires officers be age 55 with 25 years before they can receive their pension. The age requirement was removed for the health care benefits, however, which means an officer could retire before age 55. He or she would not receive their monthly pension until 55, but the city would be obligated to immediately pay their health care until they reach Medicare age, currently 65.

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Health care benefits in police contract could be budget buster

New privacy concerns over government's health care website

AP Photo/Don Ryan, File FILE - This Nov. 12, 2014 file photo shows the HealthCare.gov website, where people can buy health insurance, on a laptop screen, shown in Portland, Ore. A little-known side to the governments health insurance website, HealthCare.gov, is prompting renewed concerns about privacy, just as the White House is calling for stronger cybersecurity protections for consumers.

WASHINGTON A little-known side to the government's health insurance website is prompting renewed concerns about privacy, just as the White House is calling for stronger cybersecurity protections for consumers.

It works like this: When you apply for coverage on HealthCare.gov, dozens of data companies may be able to tell that you are on the site. Some can even glean details such as your age, income, ZIP code, whether you smoke or if you are pregnant.

The data firms have embedded connections on the government site. Ever-evolving technology allows for individual Internet users to be tracked, building profiles that are a vital tool for advertisers.

Connections to multiple third-party tech firms were documented by technology experts who analyzed HealthCare.gov, and confirmed by The Associated Press. There is no evidence that personal information from HealthCare.gov has been misused, but the number of outside connections is raising questions.

"As I look at vendors on a website...they could be another potential point of failure," said corporate cybersecurity consultant Theresa Payton. "Vendor management can often be the weakest link in your privacy and security chain."

A former White House chief information officer under President George W. Bush, she said the large number of outside connections on HealthCare.gov seems like "overkill" and makes it "kind of an outlier" among government websites.

The privacy concerns come against the backdrop of President Barack Obama's new initiative to protect personal data online, a highlight of his State of the Union message scheduled for Tuesday night. The administration is getting the health care website ready for the final enrollment drive of 2015, aiming to have more than 9 million people signed up by Feb. 15 for subsidized private coverage.

Medicare spokesman Aaron Albright said outside vendors "are prohibited from using information from these tools on HealthCare.gov for their companies' purposes." The government uses them to measure the performance of HealthCare.gov so consumers get "a simpler, more streamlined and intuitive experience," he added.

The administration did not explain how it ensures that privacy and security policies are being followed.

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New privacy concerns over government's health care website

Health care jobs spike with Medicaid expansion

Through the first 11 months of 2014 the first full year of Medicaid expansion in Ohio statewide employment in health care and social assistance, which includes jobs at hospitals, doctors offices and other facilities providing medical care, rose by about 7,000 positions, according to figures from the Ohio Department of Jobs and Family Services.

Thats up about 1 percent from job growth in the health sector for the same period a year ago, and just slightly below the full-year forecast. The figures, however, do not reflect jobs created in other sectors that benefit indirectly from increased health care spending.

The influx of federal dollars into Ohio to fund the expansion of Medicaid was projected to boost employment in health care and other industries by at least 9,000 jobs by the end of last year, according to economic impact studies from the Urban Institute and Regional Economic Modeling Inc.

When additional funds are coming in for any particular type of programyou would expect to see job growth tied to additional expenditures in those areas, said Richard Stock, director of the Business Research Group at the University of Dayton. That would be as true for something like the Affordable Care Act and increases in Medicaid spending as for (U.S. Department of Defense) spending.

Still, its hard to say exactly what percentage of job growth can be attributed to the $2.5 billion in extra Medicaid funds Ohio accepted to expand Medicaid eligibility.

An improving economy has boosted overall job growth and is at least partly responsible for growth in the health care sector.

But the addition to Ohios Medicaid rolls of more than 450,000 residents, who became newly eligible last year under guidelines granting coverage to most adults earning up to 138 percent of the federal poverty level, has created a huge group of new health care consumers who are driving hiring at local medical practices.

Over the past two years, our Medicaid volume has increased steadily.and we have services in place that required additional staffing, said Holly Card, director of oncology services at Dayton Physicians Network, the largest oncology practice in the local area.

Robin Arthur, a recent hire, said she left Five Rivers Medical Surgical Health Center in Dayton last September because of a surge in new Medicaid patients that placed increased demands on staff.

We enrolled a lot of Medicaid patients last year at Five Rivers because of expansion, and I became more of a case manager than a specialist, Arthur said. I came here (Dayton Physicians) because I wanted to be able to focus on oncology infusion.

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Health care jobs spike with Medicaid expansion

Report: Health care supports other jobs in Kansas, Shawnee County

Health care in Kansas supports far more jobs than just doctors and nurses, according to a report from the Kansas Hospital Association.

About 4,403 people worked for Stormont-Vail HealthCare as of the end of the fiscal year in September, spokeswoman Nancy Burkhardt said. Ben Bauman, spokesman for St. Francis, said about 1,600 people are employed at St. Francis Health.

KHAs last report on Shawnee County, in 2013, found every 100 health care jobs generate an average of an additional 55 jobs, though not all health care jobs had the same impact. By that calculation, employees at Stormont and St. Francis would support about 3,300 additional jobs.

Hospital jobs generate more additional employment than most other health care jobs, possibly reflecting the relatively high level of pay their workers receive compared with people in fields like home health care.

Stormont paid out $268,349,385 in salaries throughout its system in fiscal year 2014, Burkhardt said. A 2013 KHA report showed each $1 in health care salaries generates roughly an additional 40 cents in other economic activity in Shawnee County as workers spend their salaries on other services and goods. If that multiplier is still valid, workers at Stormont would generate about $106 million in additional economic activity.

Recent salary data for St. Francis wasnt available Monday.

Statewide, 221,501 people worked in health care. KHA found health care supported 357,408 other jobs, or about 61 additional jobs for every 100 health jobs. Employees in the health sector also drove about $5.5 billion in retail sales and $57.4 million in other economic activity.

Health care was the fourth-largest sector by employment in Kansas, behind services other than health, retail and government, and accounted for 11.6 percent of all jobs in the state. Health care tends to have a bigger impact in rural communities, where local government and schools may be the only entities that employ more people, according to KHA.

Health care spending in Kansas in 2013 was about $21.7 billion, including about $11.9 billion in salaries. That averages out to about $53,859 per worker, though an average conceals the broad discrepancy between pay for a home health aide and an elite surgeon, for example.

The largest employers within health care were hospitals, where 84,210 people worked. Nursing and residential care facilities were a distant second, with 45,228 employees, followed by doctors offices, 23,787; health and personal care stores, 10,977; and dentists offices, 9,271.

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Report: Health care supports other jobs in Kansas, Shawnee County

Analysis: The next big health-care shift is coming

(Bloomberg View) -- The next big shift is coming in U.S. health care, and Republicans are doing their best to speed it up. What's not clear is how carefully they've thought through the consequences.

A defining feature of U.S. health-care policy is that most Americans get insurance through their employers. That has frustrated liberals' attempts to rally voters behind Obamacare's modest reforms, let alone the single-payer model that works so well in most developed countries: The average person has no incentive to support changes that might jeopardize their situation.

But judging by the trend of the past few years, Americans with job-based coverage could soon become a minority. The chart below shows the percentage of Americans younger than 65 with health insurance provided by their employer. (Almost everyone 65 and older is covered by Medicare.) There has been a gradual but steady decline, from 59.2 percent in 2009 to 57.1 percent in 2013.

The shift away from employer-based insurance has been pronounced for most age groups. Among those 55 to 64, for example, the share with employer-based coverage fell almost four percentage points from 2009 to 2013. There's one exception: young adults, who benefited from an Obamacare provision that forces insurers to keep children on their parents' plan until age 26.

Other parts of Obamacare will accelerate the trend. The Congressional Budget Office estimates that in 2018, 8 million fewer people will have employer-based coverage than would have without the law. If 8 million fewer people 64 or younger had been covered by their employer in 2013, the share of non-elderly Americans with job-based health insurance would have been 54.1 percent.

That drop will be even greater if Republicans succeed at narrowing the category of workers to whom companies must offer affordable health insurance or pay a fine. The protection now extends to anyone at a company with 50 or more employees who works at least 30 hours a week; Republicans want to raise that threshold to 40 hours.

A still greater change would come from repealing the employer mandate entirely, which Republicans may try to pursue if they get more leverage. The latest Supreme Court challenge for Obamacare may give them that opportunity.

It's debatable whether Republicans have considered the consequences of that push. In the short term, it would reduce the regulatory exposure of U.S. businesses, which explains why Chamber of Commerce President Thomas Donohue highlighted the campaign in a speech last week.

Over the long term, who wins and who loses gets a lot murkier. Republicans who support decoupling insurance from employment, on the grounds that doing so would free people to change jobs more easily, may see the decline of employer-based insurance as something to cheer.

But there's another view, one that's less happy for Republicans. The sooner employer-based coverage becomes something that only a minority of Americans enjoy, the greater the challenge to the psychological barricades against government-sponsored health care -- whether that's Obamacare, as my colleague Megan McArdle has noted, or something more sweeping.

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Analysis: The next big health-care shift is coming

'Molecular scissors' could prevent genetic diseases before conception

20 hours ago by Vicky Just The technique can edit DNA at the point of fertilisation, speeding up the process of genetic research

Scientists from our Department of Biology & Biochemistry have developed a new technique that will streamline biomedical research and could in the future prevent genetic diseases before the moment of conception.

In a study published in the Nature Group journal Scientific Reports, the scientists used 'molecular scissors' that can edit the DNA of either the egg or sperm of mice during fertilisation.

The researchers used the enzyme Cas9 to cut a precise point in the genome, enabling them accurately to inactivate a specific gene. This allows scientists to study specified gene function in mice by creating a 'knock-out' in closer to one month rather than the six required using conventional techniques.

This powerful tool should accelerate biomedical research and promises to reduce the number of animals used in experiments to answer fundamental medical questions.

Dr Tony Perry, the study's senior author at the University's Laboratory of Mammalian Molecular Embryology, explained: "We're really excited about this research. Previously, this technique had been demonstrated with established embryos but we've shown that we can accurately edit genes in the sperm or egg around the time of fertilisation, just as the embryo is starting to develop.

"Cas9 works by cutting the DNA at a precise point in the genome. The cell repairs this cut but chews the frayed ends before rejoining them, destroying the function of the gene.

"The technique has many exciting potential applications. It could help to provide disease resistance to livestock or perhaps provide a method for preventing serious genetic conditions in humans at the point of conception - for example by allowing carriers of life-threatening genetic conditions such as cystic fibrosis to conceive healthy babies without the risk of passing on the disease."

Researchers anticipate that the method could also be used to enable the transplant of organs of some large animals into humans without the problem of rejection, by making the organs immunologically invisible.

Dr Perry added: "This is a dream for transplant surgeons and patients awaiting immunologically matched organs. It means that one day it may be possible to transplant these engineered organs - even if only until a suitable human one is found - and save lives."

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'Molecular scissors' could prevent genetic diseases before conception

Gene Therapy – Preferable sites and orientations of …

Gene Therapy advance online publication 15January2015; doi: 10.1038/gt.2014.124

First-generation (E1 deleted) adenovirus vectors (FG AdVs), which lack the E1 and E3 regions, are popularly used in basic studies to elucidate gene functions, and have been employed for gene therapy.1, 2, 3, 4 Because the DNA fragments of up to about 7 kilobases (kb) in total can be inserted into the AdV genome, the AdVs are frequently used to produce two proteins simultaneously from two independent transgenes expressing both the target gene and the reporter gene, for example. In the studies using the cultured cells and in the animal experiments, the GFP and luciferase are used as the reporters. Recently, positron emission tomography has clinically been used in patients for diagnoses and in experimental animal models. Therefore, the AdVs containing both the therapeutic gene and the positron emission tomography reporter gene would be valuable in the gene therapy fields, because the therapeutic effects, the vector duration and distribution can simultaneously be monitored.5, 6, 7, 8 Probably one would wish for high-titer AdVs with the highest expression for the therapeutic gene and with the second highest for the reporter gene not causing any trouble, if the insertion sites and orientations in the AdV genome can be chosen. However, the titers and the expression levels of the AdVs may considerably be influenced by the sites and orientations of the transgenes. Such information may be very valuable for construction of the best vector, especially in the vector containing both the therapeutic gene and the reporter gene.

The simultaneous expression of two genes could be achieved by inserting the two genes into the E1 site under the control of a single prompter using the internal ribosomal entry sites or using porcine teschovirus-1 2A.9, 10 In the former approach, the expression of the second gene might be influenced by the sequences between internal ribosomal entry sites and its initiation codon, and in the latter, the manipulation is necessary to remove the stop codon of the first gene and to adjust the frames of the two genes. When two genes driven by the independent promoters are inserted into the E1 site, they might interfere with each other. However, when two independent expression units are inserted in different sites in the AdV genome, no interference occurs. Moreover, the advantage of this approach is that the main target gene can easily be changed using the AdV cassette that already contains the reporter gene.

There are three insertion sites and two orientations: a transgene can be inserted into the AdV genome by substitution of the E1 or E3 gene and by simple insertion at a position upstream of the E4 gene. Therefore, there are six different possible sites/orientations for any given transgene. Moreover, not only the potent promoters such as EF1 but also tissue-specific promoters such as -fetoprotein (AFP) can also be employed. Although the studies examining which sites/orientations are superior to others are practically important, they have been very limited11, 12 and systematic analyses have not been reported so far.

As it is known that the expression level of a transgene varies considerably depending on the site in the cell chromosome of the human genome, the phenomenon is called the position effect.13, 14 Although CG-methylation in the cell chromosome is clearly one reason, it is not observed in the AdV genome. Therefore, it would be of interest to examine whether the position effect might also be observed similarly in the AdV genome for the potent promoter and for the tissue-specific promoter.

FG AdVs retain almost all viral genes. They are normally not expressed in the target cells, because E1A protein, the essential transactivator for expression of all other viral genes, is not present. However, there is one report of splicing of aberrant mRNAs from the inserted foreign genes to a viral gene.15 In this case, the aberrant mRNAs are transcribed by strong foreign promoters and produce transgene-viral gene fusion proteins, which elicit strong immune responses. However, it is not known whether the production of the aberrant gene product between the inserted transgene and viral gene is rare or not.

In this study, we examined the AdV titers and expression levels of an identical transgene inserted at the E1, E3 and E4 sites. We used three transgenes, namely, GFP, LacZ and Cre, and two promoters, namely, the potent EF1 promoter and the cancer-specific AFP promoter, and attempted to construct AdVs using all combinations, that is, 18 AdVs, and succeeded in constructing 17 of them. We found that insertion at the E1 and E4 sites yielded mostly high titers, whereas the one at the E3 yielded variable titers. Surprisingly, four aberrantly spliced mRNAs between the transgenes and viral genes were found in the vector obtained by insertion at the E3 site, which was probably the reason for the very low titers. As for the expression levels, clear differences were observed among the vectors obtained with insertion at the E1, E3 and E4 sites despite using the identical transgene, indicating that the position effect was certainly present for the AdV genome and that aberrant splicing may, at least in part, explain this effect. We also propose a strategy to avoid generation of the aberrantly spliced mRNAs.

We first examined whether the vector titers were influenced by the site/orientations of the transgenes containing a potent EF1 promoter. Towards this end, we attempted to construct six GFP-expressing (EF-GFP) and six LacZ-expressing (EF-LacZ) vectors in all possible combinations, that is, the E1, E3 and E4 insertion sites and the two orientations ( Figure 1), and measured the vector titers (Figure 2a) (hereinafter, the vectors will be designated as per the following; the vectors containing the GFP gene and LacZ gene at the E1 insertion site and in the left orientation shall be denoted as G-E1L and Z-E1L vectors, respectively). Among the GFP-expressing vectors, high titers were obtained for G-E1L, G-E3L, G-E4L and G-E4R vectors (Figure 2a, bars 1, 3, 5 and 6), while the titer for the G-E1R vector was lower (bar 2). Notably, the G-E3R vector, that is, vector with the GFP transgene inserted in the E3 site in the rightward orientation, could not be obtained despite three independent attempts (bar 4, denote ). Therefore, although exactly the same EF1-GFP expression unit was inserted in these vectors, the sites and orientations exerted considerable influence on the vector titers and even determined whether the vector was available or not. Similar results were obtained for vectors expressing LacZ: the titers of the Z-E1L, Z-E4L and Z-E4R vectors (bars 7, 11 and 12) were high, and that of the Z-E1R vector was also low (bar 8). However, the results of insertion at the E3 site differed for GFP and LacZ. The titer ratio of Z-E3L was significantly lower than that of G-E3L (compare bars 3 and 9, described later), and the Z-E3R vector was available, although its titer was extremely low (bar 10). Therefore, the GFP gene and LacZ gene themselves influenced the vector titers.

The FG AdV structures of six different site/orientations in all possible combinations. The box containing pro, gene and pA represents the expression unit and the arrows show the orientation of transcription. pro, EF1 and AFP promoter; gene, GFP, LacZ and Cre; pA, rabbit -globin polyadenylation signal. For example, the vector containing the transgene at the E1 insertion site and in the left orientation is denoted as E1L.

Titers of the virus vectors containing identical expression units. (a) Virus titers of the AdVs containing the EF1 promoter. The AdV genomes transduced into the HuH-7 cells were measured 3 days post infection. The virus titers were calculated relative to the copy numbers of the AdVs.16 The titer of the E1L vector was set as 1; G-E1L, 8.3 108 relative virus titer (rVT)/ml, L-E1L, 5.0 109 rVT/ml. indicates that G-E3R could not be obtained. (b) The titers of the virus vector containing Cre gene driven by the AFP promoter. E1L vector was used as the control. *P<0.05, **P<0.01.

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Gene Therapy - Preferable sites and orientations of ...

Impatient Consumers and the Hotel Industry – Futurist Speaker – Video


Impatient Consumers and the Hotel Industry - Futurist Speaker
Consumer impatience and the impact on the hotel industry. Most customers will leave a website if it takes more than 5 seconds to load, the same principle app...

By: Patrick Dixon Futurist Keynote Speaker for Industry Conference

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Impatient Consumers and the Hotel Industry - Futurist Speaker - Video

Faking Murders And Stealing Bitcoin: Why The Silk Road Is The Strangest Crime Story Of The Decade

Illustration of Day 1 of the Ross Ulbricht trial. (Illustration by Susie Cagle)

To his family, he was former Eagle Scout Ross Ulbricht. To his housemates, he was Joshua Terrey. To the U.S. government, hes The Dread Pirate Roberts the criminal mastermind who created and operated the billion dollar black market website Silk Road. His trial began on January 13.

The prosecutions story of an exceptionally bright and smart kid who grew up to become the criminal head of a global black market is in itself interesting enough to provide Hollywood with a half a dozen movies. But even if the government proves these allegations, this character arc would actually be one of the more normal and mundane things about this story.

Here are a few of the bizarre twists and turns in the investigation of The Dread Pirate Roberts, Silk Road, and Ross Ulbricht:

An Undercover Agent Basically Worked The Helpdesk For Silk Road

Illustration by Susie Cagle

In July of 2013, Jared Der-Yeghiayan, a special agent with Homeland Security Investigations, took over the account of a Silk Road moderator under the alias cirrus. By the time of Ulbrichts capture, Der-Yeghiayan had been undercover for about two months.

During his time undercover, Der-Yeghiayans worked for 10 to 12 hours each day, and was paid about one thousand dollars in bitcoin from DPRs Silk Road account every week.

Essentially, he was a helpdesk worker for an online black market. But it was his contact with Dread Pirate Roberts that led to a sting operation to arrest Ulbricht. The Federal Government Faked A Hit Ordered By The Dread Pirate Roberts

Curtis Clark Green, a semi-pro poker player in his late 40s, had a second job that his family knew nothing about he was an administrator for Silk Road who was known for frequently shared personal anecdotes and detailed advice on using and dealing illegal substances.

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Faking Murders And Stealing Bitcoin: Why The Silk Road Is The Strangest Crime Story Of The Decade

Silhouette Man Running Light In A Tunnel Escape Freedom Concept. Stock Footage – Video


Silhouette Man Running Light In A Tunnel Escape Freedom Concept. Stock Footage
"This is a preview video. Download high quality footage at: http://www.pond5.com/stock-footage/27819849?ref=silverstepper More stock media here: http://www.pond5.com?ref=silverstepper Editing...

By: Agata Dendrow

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Silhouette Man Running Light In A Tunnel Escape Freedom Concept. Stock Footage - Video

Arun Jaitley speaks on media freedom and responsibilities in JS Verma memorial lecture in Delhi – Video


Arun Jaitley speaks on media freedom and responsibilities in JS Verma memorial lecture in Delhi
Central Minister Arun Jaitley said that the the age of ban on media organisations was over. Jaitley said this while delivering the first Justice J S Verma memorial lecture in New Delhi. Watch...

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Arun Jaitley speaks on media freedom and responsibilities in JS Verma memorial lecture in Delhi - Video