Multiplex qPCR: Criteria for Success – Video

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Multiplex qPCR: Criteria for Success
Current best practices for gene expression analysis by qPCR recommend the use of multiple normalizers and, potentially, a second gene of interest (GOI) assay. For samples with limited nucleic acid content, such an experiment may only be achieved by performing multiplexed qPCR assays. Even when working with abundant nucleic acids, multiplexing can save time and money. Achieving successful multiplex assays depends upon several criteria, including using robust assays, correct reagent and target concentrations, and appropriate cycling parameters. Using data from several assays, we demonstrate how PrimeTime® qPCR Assays simplify a 4-plex assay system, and discuss factors that influence the success of using these assays in a multiplex format. We also compare the use of master mixes assembled de novo to commercially available reagents and discuss their effects on multiplex assays. About the Speaker: Scott Rose, PhD, is the Director of Molecular Genetics at Integrated DNA Technologies. He leads a group of scientists involved in numerous projects and research collaborations in areas such as qPCR, RNAi, antisense, RNase H2--based detection, and synthetic biology. The focus of his postdoctoral research was transcription-based gene regulation using the pancreas as a model system. For his PhD in Biochemistry, he studied RNA processing, concentrating on RNA splicing and polyadenylation.From:idtdnabioViews:5 0ratingsTime:01:09:12More inScience Technology

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Multiplex qPCR: Criteria for Success - Video

Rachel — from Raleigh, North Carolina — Chemistry Major at College of Charleston – Video

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Rachel -- from Raleigh, North Carolina -- Chemistry Major at College of Charleston
No other city beats the beauty, rich history and energetic vibe of Charleston. With easy access to inspiring arts and cultural affairs, world-renowned festivals, outdoor recreation and great beaches, this place has it all. You #39;ll be glad you #39;re here. About Rachel #39;s major -- Chemistry: In addition to serving the needs of chemists and biochemists, Chemistry and Biochemistry courses serve as the foundation for many other disciplines, including but not limited to Atmospheric Science, Biology, Dental School, Environmental Science, Forensics, Geology, Material Science, Medical School, Medicinal Chemistry, Molecular Biology, Patent or Environmental law, Pharmaceutical Sciences, Pharmacology, and Veterinary School. Our students learn fundamental concepts, facts and theories needed to investigate chemical and biochemical problems. Our students also gain insight into how those concepts, facts and theories are relevant to contemporary life. The study of chemistry has been part of the curriculum at the College from its earliest days. One of the earliest versions of the periodic chart, "Synoptic Tables", similar to the periodic tables adopted and used today, was developed by Dr. Lewis Reeve Gibbs (1810-1894, Professor of Mathematics and Natural Philosophy at the College of Charleston).From:CollegeofCharlestonViews:22 1ratingsTime:01:16More inEducation

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Rachel -- from Raleigh, North Carolina -- Chemistry Major at College of Charleston - Video

Dato’ Dr. Halo-N’ (MALAYSIA) receives Gusi Peace Prize 2012 – Video

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Dato #39; Dr. Halo-N #39; (MALAYSIA) receives Gusi Peace Prize 2012
Dato #39; Dr. Haji Ahmad Laksamana Bin of Malaysia Omar receives Gusi Peace Prize 2012 For his contributions as the first Al-Quranic Scientist of the World in the Field of Research(Biochemistry). By interpreting the meaning of the Noble Quran, he made a significant breakthrough in the area of Biochemistry by successfully establishing five formula and theories to the field of natural products.From:gusipeaceprizeViews:2 0ratingsTime:03:42More inPeople Blogs

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Dato' Dr. Halo-N' (MALAYSIA) receives Gusi Peace Prize 2012 - Video

Education of the Heart and Mind: Santa Ono at TEDxEmory 2012 – Video

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Education of the Heart and Mind: Santa Ono at TEDxEmory 2012
Santa J. Ono is a biomedical scientist and university administrator. He currently serves as Senior Vice President for Academic Affairs and University Provost at the University of Cincinnati. Prior to his move to UC, Santa served as Vice Provost for Academic Initiatives and Deputy to the Provost and then Senior Vice Provost for Undergraduate Academic Affairs at Emory University. He was educated at the University of Chicago, McGill, and Harvard, and was a Helen Hay Whitney Fellow in Biochemistry Molecular Biology at Harvard. He has taught at Johns Hopkins, Harvard, University College London, and Emory. In this talk, Ono examines the path to Global Citizens through American Higher Education. AboutTEDx, x = independently organized event In the spirit of ideas worth spreading, TEDx is a program of local, self-organized events that bring people together to share a TED-like experience. At a TEDx event, TEDTalks video and live speakers combine to spark deep discussion and connection in a small group. These local, self-organized events are branded TEDx, where x = independently organized TED event. The TED Conference provides general guidance for the TEDx program, but individual TEDx events are self-organized.* (*Subject to certain rules and regulations)From:TEDxTalksViews:3 2ratingsTime:20:30More inEducation

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Education of the Heart and Mind: Santa Ono at TEDxEmory 2012 - Video

Allosteric regulation/ Sequential Allostery / Allosteric model / allosteric regulation of enzyme – Video

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Allosteric regulation/ Sequential Allostery / Allosteric model / allosteric regulation of enzyme
In biochemistry, allosteric regulation is the regulation of an enzyme or other protein by binding an effector molecule at the protein #39;s allosteric site (that is, a site other than the protein #39;s active site)From:Biotech ReviewViews:0 0ratingsTime:00:27More inScience Technology

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Allosteric regulation/ Sequential Allostery / Allosteric model / allosteric regulation of enzyme - Video

Nucleosome Rock Star Scientist Karolin Luger at Colorado State University – Video

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Nucleosome Rock Star Scientist Karolin Luger at Colorado State University
Karolin Luger, University Distinguished Professor and Investigator for the Howard Hughes Medical Institute at Colorado State University, can add another award to her list of accomplishments: National Lecturer for the Biophysical Society, which is one of its most prestigious honors. Her research focuses on the structure, function and assembly of the nucleosome, using a wide variety of biophysical approaches. Her work is now cited in nearly every modern textbook of biochemistry and molecular biology. For more videos about CSU, check out Colorado State University #39;s YouTube channel at http://www.youtube.comFrom:ColoradoStateUnivViews:2 0ratingsTime:02:50More inScience Technology

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Nucleosome Rock Star Scientist Karolin Luger at Colorado State University - Video

Lefkowitz Responds to Winning Nobel Prize in Chemistry – Video

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Lefkowitz Responds to Winning Nobel Prize in Chemistry
At a press conference Oct. 10, 2012, at the Duke University School of Nursing, Dr. Robert Lefkowitz reacts to the announcement that morning that he was awarded the Nobel Prize in Chemistry along with Stanford #39;s Brian Kobilka. Lefkowitz is a professor of biochemistry, immunology and medicine at Duke, and also a basic research cardiologist in the Duke Heart Center. He is a Howard Hughes Medical Institute investigator. Learn more here: spotlight.duke.eduFrom:DukeViews:1 0ratingsTime:47:24More inEducation

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Lefkowitz Responds to Winning Nobel Prize in Chemistry - Video

Brian Malkin discusses some differences in orphan drug regulations in us vs europe – Video

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Brian Malkin discusses some differences in orphan drug regulations in us vs europe
Brian J. Malkin is a partner in the Washington, DC office of Frommer Lawrence Haug LLP. Mr. Malkin worked as a regulatory counsel at the US Food and Drug Administration in both the Center for Drug Evaluation and Research and the Office of the Commissioner, specializing in the areas of intellectual property and exclusivity related to the Hatch-Waxman Act, new product evaluations, and compliance issues related to clinical investigations. In private practice, Mr. Malkin works with pharmaceutical and biotechnology companies to develop strategies regarding regulatory requirements and clinical development plans. Mr. Malkin has a bachelor #39;s degree in biochemistry from the University of Maryland, and a JD from George Washington University. Brian is also editor and contributor to http://www.fdalawyersblog.com, an excellent blog on the inner workings of the regulatory process.From:rarediseasereport2Views:1 0ratingsTime:00:59More inPeople Blogs

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Brian Malkin discusses some differences in orphan drug regulations in us vs europe - Video

Freeman Hrabowski and Michael Summers: Increasing Underrepresented Minorities in Science Research – Video

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Freeman Hrabowski and Michael Summers: Increasing Underrepresented Minorities in Science Research
Drs. Hrabowski and Summers discuss why programs that support and graduate minority students in STEM subjects are necessary. They also describe their Meyerhoff Scholars Program at UMBC, a program that has been very successful and which could be replicated at other schools. About the speakers: Dr. Hrabowski has been President of the University of Maryland, Baltimore County for 20 years. During his tenure, UMBC has become one of the top schools in the country for graduating STEM majors of all races, including African Americans. Dr. Summers is a Professor of Chemistry and Biochemistry at UMBC and a long-time supporter of the Meyerhoff Scholars Program. His lab uses NMR to study the structure of HIV proteins and undergraduate students, including many Meyerhoff Scholars, have been key contributors to his research.From:ibiomagazineViews:50 0ratingsTime:12:13More inScience Technology

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Freeman Hrabowski and Michael Summers: Increasing Underrepresented Minorities in Science Research - Video

Mindfulness

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Mindfulness Ayurveda Retreat with Mudita Institute in Byron Bay
Mindfulness Ayurveda Health Retreat with Mudita Institute in Byron Bay. For more information see: http://www.muditainstitute.com. At the Mudita Mindfulness Ayurveda Health Retreats you will enjoy a transformative week of teachings, rest, relaxation, healing Ayurvedic food, cooking demonstrations, daily meditation and yoga and therapeutic treatments and consultations. Learn how to cook, eat and live in a way that supports a strong metabolism and immune system. Learn how what we eat directly affects our state of mind as well as our state of health. Learn simple mindfulness practices for improving your capacity to manage stress, anxiety, anger and other difficult emotions. And, most importantly, learn how to be kinder to yourself in your approach to health and happiness. If you are interested in your health and happiness it is more than likely you #39;re a little confused. Why? Because you #39;re bombarded with huge amounts of information every day that is often contradictory or constantly changing based on the latest research. At this retreat, we #39;ll clear up some of the confusion. The things we teach just make sense. They are based on the fundamental laws of nature and these laws do not change. Ever. So the recommendations do not change. There will be no talk of superfoods and no bombarding you with micronutrient guff and biochemistry. The things we teach are easy, understandable, enjoyable, do-able and sustainable. No fuss. No stress. Nothing freaky... just grandmother wisdom that ...From:MuditaInstituteViews:2 0ratingsTime:02:06More inEducation

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Mindfulness