Woburn, MA (PRWEB) October 09, 2013
PathoGenetix, Inc., a commercial-stage developer of an automated system for rapid bacterial identification, will present new research today demonstrating the use of Genome Sequence Scanning (GSS) technology to confirm and identify multiple serovars of Salmonella in enriched food samples in less than five hours. The data, included in a poster presentation at the 4th American Society for Microbiology (ASM) Conference on Salmonella in Boston, add to a growing body of research demonstrating the use of PathoGenetixs proprietary genotyping technology to reliably identify pathogens of public health and food safety significance, including Salmonella and Shiga toxin-producing E. coli (STECs).
The study evaluated the use of GSS in molecular serotyping and sub-typing of Salmonella, and as a tool for simultaneous detection of multiple serovars of Salmonella in complex mixtures. The study results show that GSS can be used to infer the serotype of an unknown Salmonella strain, based on the location of the strain on the GSS tree and the identity of its neighbors. The results also demonstrate the ability of GSS to shorten the time to just five hours for pathogen subtyping and serotype determination from an enriched food sample, including those containing multiple serovars.
Because Genome Sequence Scanning is culture independent, and fully automated from sample preparation to final report, the technology greatly reduces the time, complexity and skill required when compared to other molecular and next generation sequencing (NGS) identification approaches. The strain-type information provided by GSS is comparable to pulsed field gel electrophoresis (PFGE), the current standard for pathogen typing in foodborne outbreak investigation and response. As a result, GSS offers a powerful new tool for epidemiological investigations and outbreak monitoring that can enable quicker decisions affecting food safety and public health. The GSS technology will be commercially available in 2014 in the RESOLUTION Microbial Genotyping System.
According to the American Society of Microbiology, Salmonella infections continue to be a major public health problem in many parts of the world. In the U.S., Salmonella is the leading cause of foodborne illnesses leading to hospitalization and death. The Salmonella genus has more than 2,500 serotypes or serovars, based on the antigens that the organism presents on its surface. In the U.S., Salmonella Enteritidis and Salmonella Typhimurium are the most common serotypes, accounting for half of all Salmonella infections in people.
The 4th ASM Conference on Salmonella brings together scientists from a variety of backgrounds to present the most recent research and discoveries in the field, including new approaches in diagnosis, treatment and prevention of infections. PathoGenetixs research, entitled Evaluation of Genome Sequence Scanning technology for molecular (sub)-serotyping of Salmonella and simultaneous detection of multiple Salmonella serovars in complex mixtures is being presented in a poster session on October 9.
The research tested the strain typing capability of GSS using more than 400 strains and genome sequences representing the most frequently encountered Salmonella serovars from food products associated with human illness. The results show that Genome Sequence Scanning clustered Salmonella strains into serovar-specific branches on the GSS tree, clearly demarcating the major serovars from each other. Polyphyletic lineage serovars like S. Newport and S. Saintpaul formed more than one distinctly separated branch on the tree, reflecting the genetic heterogeneity within these serovars. GSS assigned correct serovar designations to strains untypeable by conventional serotyping and to antigenic variants of serovars based on genetic similarity. Genome Sequence Scanning also reliably detected the presence of up to three different serovars of Salmonella in the presence of complex background flora, demonstrating the technologys ability to provide strain information directly from complex mixtures.
***
About PathoGenetix, Inc.
PathoGenetix, Inc. is a commercial-stage developer of an automated system for rapid bacterial identification from complex samples. PathoGenetix is a venture-backed company that has received more than $50 million in technology development funding from the Department of Homeland Security. The core Genome Sequence Scanning (GSS) technology analyzes DNA from an enriched biological samplewithout the need for a cultured isolateand provides results in five hours. GSS has broad applicability in food safety, industrial microbiology, and clinical diagnostics and research. The GSS technology will be available in the RESOLUTION Microbial Genotyping System in 2014 for use in food safety testing and foodborne illness outbreak investigations. Learn more at http://www.pathogenetix.com.
Read more:
PathoGenetix Research Shows Rapid Identification of Multiple Salmonella Serovars in Food Samples
- Monoclonal antibody production by hybridoma technique explained - October 11th, 2010 [October 11th, 2010]
- Humoral immunity - October 11th, 2010 [October 11th, 2010]
- Introduction to Microbiology Culture Techniques - August 27th, 2011 [August 27th, 2011]
- Microbiology 2: Prokaryotes and Eukaryotes - August 27th, 2011 [August 27th, 2011]
- Microbiology - August 27th, 2011 [August 27th, 2011]
- Human Physiology : What Is Microbiology? - August 27th, 2011 [August 27th, 2011]
- Microbiologist - August 27th, 2011 [August 27th, 2011]
- Lecture 18 Introduction to Microbiology - August 27th, 2011 [August 27th, 2011]
- microbiology final review - August 27th, 2011 [August 27th, 2011]
- A Day in the Life - Microbiologist - August 27th, 2011 [August 27th, 2011]
- Microbiology Open House - August 27th, 2011 [August 27th, 2011]
- Microbiology Dilution series demonstration - August 27th, 2011 [August 27th, 2011]
- MCQ in Microbiology : DoctorKC - August 31st, 2011 [August 31st, 2011]
- Microbiology Movie - September 23rd, 2011 [September 23rd, 2011]
- Microbiology Pure Culture Technique - September 23rd, 2011 [September 23rd, 2011]
- Wastewater Microbiology - September 23rd, 2011 [September 23rd, 2011]
- Microbiology and Parasitology Case Study Mycobacterium Tuberculosis - September 23rd, 2011 [September 23rd, 2011]
- Lecture 19 Microbiology - September 23rd, 2011 [September 23rd, 2011]
- Pharmaceutical Microbiology - September 23rd, 2011 [September 23rd, 2011]
- Quest Diagnostics Department of Molecular Microbiology Summer Blast 2009 - September 23rd, 2011 [September 23rd, 2011]
- Koch's Postulates: an introduction to microbiology - September 23rd, 2011 [September 23rd, 2011]
- Microbiology: Streaking for Isolation - September 23rd, 2011 [September 23rd, 2011]
- Anatomy and Physiology, Microbiology, Pathophysiology Videos - September 23rd, 2011 [September 23rd, 2011]
- Duke Graduate Program in Molecular Genetics and Microbiology - September 23rd, 2011 [September 23rd, 2011]
- Hot Topics in Diagnostic Microbiology: Chlamydia and Asthma - September 23rd, 2011 [September 23rd, 2011]
- Tim Day - Undergraduate Microbiology Research - September 23rd, 2011 [September 23rd, 2011]
- Microbiology Practice Test Chapter 1 part 2/3 - September 23rd, 2011 [September 23rd, 2011]
- Welcome to Microbiology at NUI Galway - September 23rd, 2011 [September 23rd, 2011]
- Microbiology degree, college program interview from drkit.org - September 24th, 2011 [September 24th, 2011]
- Microbiology: Aseptic Transfer - September 24th, 2011 [September 24th, 2011]
- Improving Soils with Microbiology - September 24th, 2011 [September 24th, 2011]
- Microbiology Practice Test Chapter 1 part 1/3 - September 24th, 2011 [September 24th, 2011]
- Microbiologists Careers Overview - September 24th, 2011 [September 24th, 2011]
- Biology 1AL - Lecture 2: Lab 2 - Enzymes, Microbiology-Vibri - September 25th, 2011 [September 25th, 2011]
- Day in the Life - Microbiology / Virology - Prof Bill Rawlinson - September 25th, 2011 [September 25th, 2011]
- Laboratory Manager Job (Microbiology) - London, UK - September 25th, 2011 [September 25th, 2011]
- Assuring Quality in Microbiology Diagnostics - September 25th, 2011 [September 25th, 2011]
- Microbiology Testing Innovations for China and The World - September 25th, 2011 [September 25th, 2011]
- TRINITY V3 Industrial Microbiology System - September 26th, 2011 [September 26th, 2011]
- Microbiology Advancing Translational Medicine - September 27th, 2011 [September 27th, 2011]
- Advancing Microbiology Testing Technology - September 27th, 2011 [September 27th, 2011]
- Biology 1AL - Lecture 2: Lab 3: Enzymes, Microbiology-Vibrio - September 28th, 2011 [September 28th, 2011]
- Microbiological Fuel Contamination - September 28th, 2011 [September 28th, 2011]
- Microbiology Streaking Plates - September 30th, 2011 [September 30th, 2011]
- Lecture 2: Essentials of Microbiology, Introduction to Microfluidics - October 2nd, 2011 [October 2nd, 2011]
- Healthwatch: PRHC Microbiology Lab - October 6th, 2011 [October 6th, 2011]
- SOIL not DIRT - Dr Elaine Ingham talks Soil Microbiology - October 7th, 2011 [October 7th, 2011]
- The Microbiology of Reptiles - Video - October 13th, 2011 [October 13th, 2011]
- Microbiology - Video - October 13th, 2011 [October 13th, 2011]
- Safer Sprouts - Part 6 - Sampling and MIcrobiological Testing - Video - October 14th, 2011 [October 14th, 2011]
- Microbiology movie - Video - October 14th, 2011 [October 14th, 2011]
- Kaplan's USMLE Step 1 Program Preview: Microbiology - Video - October 14th, 2011 [October 14th, 2011]
- DNA Replication Process Animation from Microbiology 2e - Video - October 15th, 2011 [October 15th, 2011]
- Rhizobium Microbiology Video - Video - October 15th, 2011 [October 15th, 2011]
- Introduction to Microbiology Dr. Ahmed Ramzy (Part 1).wmv - Video - October 15th, 2011 [October 15th, 2011]
- A tour of the Microbiology Lab - Section one - Video - October 16th, 2011 [October 16th, 2011]
- Environmental Biology, Microbiology and Chemistry through Science at UCD - Video - October 16th, 2011 [October 16th, 2011]
- Total Solutions for Microbiology Testing - Video - October 18th, 2011 [October 18th, 2011]
- US Navy Microbiologist- Dr. Rockabrand - Video - October 23rd, 2011 [October 23rd, 2011]
- Microbiology Song - Video - October 24th, 2011 [October 24th, 2011]
- Marine Microbiology Initiative Overview - Video - October 26th, 2011 [October 26th, 2011]
- A tour of the Microbiology Lab - Section Three - Video - October 26th, 2011 [October 26th, 2011]
- Great microbiologists - Video - October 26th, 2011 [October 26th, 2011]
- Microbiology: Viruses Vs Prokaryotes Vs Eukaryotes - Video - October 27th, 2011 [October 27th, 2011]
- Food Microbiology part-2 - Video - October 30th, 2011 [October 30th, 2011]
- Food Microbiology Research - Video - October 31st, 2011 [October 31st, 2011]
- Interview with a Medical Microbiologist - Video - November 1st, 2011 [November 1st, 2011]
- Food Microbiology part-1 - Video - November 8th, 2011 [November 8th, 2011]
- Food Microbiology part-3 - Video - November 8th, 2011 [November 8th, 2011]
- Microbiology Safety in the Laboratory 1 - Video - November 13th, 2011 [November 13th, 2011]
- serology, microbiology, jjmmc, davangere - Video - November 27th, 2011 [November 27th, 2011]
- Microbiology department, JJMMC, Davangere - Video - December 5th, 2011 [December 5th, 2011]
- Thermo Scientific Heratherm Microbiological Incubators - Video - December 5th, 2011 [December 5th, 2011]
- Food Microbiology: An Overlooked Frontier | Lecture 11 (2011) - Video - December 6th, 2011 [December 6th, 2011]
- A tour of the Microbiology Lab - Section Two - Video - December 7th, 2011 [December 7th, 2011]
- Microbiology catalase test - Video - December 8th, 2011 [December 8th, 2011]
- Microbiology Lab Safety - Video - December 20th, 2011 [December 20th, 2011]
- Coast to Coast Mysterious Deaths of Microbiologists - December 26th, 2011 [December 26th, 2011]
- MSU Microbiology - BPS Blues 2010 - Video - January 1st, 2012 [January 1st, 2012]
- Kiestra fully automated microbiology testing system - Video - January 4th, 2012 [January 4th, 2012]