MSc Applied Biopharmaceutical Biotechnology Entrepreneurship - Cera Wong
msc Applied Biopharmaceutical Biotechnology Entrepreneurship Cera Wong. Uploaded by nubsvideos on Mar 13 2013. Cera Wong shares her experience of studying an msc Cross Discipline Entrepreneurship Programme msc Applied Biopharmaceutical Biotechnology Entrepreneurship at Nottingham University Business School and School of Biosciences.
By: NUBSvideos
Read more here:
MSc Applied Biopharmaceutical Biotechnology
Harlem shake - Department of Biotechnology, IIT Madras
Get ready folks..!! April 13-14 it is..!
By: BiofestIITM
Read the original:
Harlem shake - Department of Biotechnology, IIT Madras - Video
Biotechnology: Transgenic V Conventional Cotton
See the way transgenic cotton varieties work to repel insects, and why they are so popular due to the massive reduction in required chemicals for insect cont...
By: CottonAustralia
Original post:
Biotechnology: Transgenic V Conventional Cotton - Video
Biyoung Biotechnology (CES SPD) eng
Biyoung BiotechnologyCES SPD eng. #30334; #27687;2 videos. Subscribe Subscribed Unsubscribe 0. 3 views. Like 0 Dislike 0. Like. Sign in to youtube. Sign in with your youtube Accountyoutube Google+ Gmail Orkut Picasa or Chrome to like #30334; #27687;s video. Sign in. I dislike this. Sign in to youtube. Sign in with your youtube Accountyoutube Google+ Gmail Orkut Picasa or Chrome to dislike #30334; #27687;s video. Sign in. About Share Add to. Sign in to youtube. Sign in with your youtube Accountyoutube Google+ Gmail Orkut ...
By: #30334; #27687;
View post:
Biyoung Biotechnology (CES
Biotechnology at Suansunandha Rajabhat University
Biotechnology at Suansunandha Rajabhat University. genetic evolution2 videos. Subscribe Subscribed Unsubscribe 0. 1 view. Like 0 Dislike 0. Like ... 334. Watch Later Biotechnology at Suansunandha Rajabhat Universityby genetic evolution 1 view; 952. Watch Later Fake a Big Shot David Hobbyby digitalrevcom 89732 views; 623. Watch Later playstation 4 and X Phoneby jon4lakers 24085 views; 625. Watch Later ifixit Pro Tech Toolkit Unboxing First Look Linus Tech Tipsby linustechtips 25301 views ...
By: genetic evolution
The rest is here:
Biotechnology at Suansunandha Rajabhat University - Video
The road to prediction with systems biotechnology - Manuel Carrondo, IBET
Manuel Carrondo, Director at IBET gives his presentation at the Cell Culture World Congress 2013 in Munich. The road to prediction with systems biotechnology...
By: biopharmachannel
Read this article:
The road to prediction with systems biotechnology - Manuel Carrondo, IBET - Video
Background:
Microbial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolysates, which are then used by oleaginous microorganisms as the carbon and energy sources to produce lipids. However, the costs of microbial lipids remain prohibitively high for commercialization. More efficient and integrated processes are pivotal for better techno-economics of microbial lipid technology.
Results:
Here we describe the simultaneous saccharification and enhanced lipid production (SSELP) process that is highly advantageous in terms of converting cellulosic materials into lipids, as it integrates cellulose biomass hydrolysis and lipid biosynthesis. Specifically, Cryptococcus curvatus cells prepared in a nutrient-rich medium were inoculated at high dosage for lipid production in biomass suspension in the presence of hydrolytic enzymes without auxiliary nutrients. When cellulose was loaded at 32.3 g/L, cellulose conversion, cell mass, lipid content and lipid coefficient reached 98.5%, 12.4 g/L, 59.9% and 204 mg/g, respectively. Lipid yields of the SSELP process were higher than those obtained by using the conventional process where cellulose was hydrolyzed separately. When ionic liquid pretreated corn stover was used, both cellulose and hemicellulose were consumed simultaneously. No xylose was accumulated over time, indicating that glucose effect was circumvented. The lipid yield reached 112 mg/g regenerated corn stover. This process could be performed without sterilization because of the absence of auxiliary nutrients for bacterial contamination.
Conclusions:
The SSELP process facilitates direct conversion of both cellulose and hemicellulose of lignocellulosic materials into microbial lipids. It greatly reduces time and capital costs while improves lipid coefficient. Optimization of the SSELP process at different levels should further improve the efficiency of microbial lipid technology, which in turn, promote the biotechnological production of fatty acid-derived products from lignocellulosic biomass.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/36
DNA, deoxyribonucleic acid it is the backbone of all living creatures on earth. It is a molecule, which is having a double helical structure and encoded with genetic instructions. It leads to the functioning of all living beings including viruses. DNA profiling is done by the forensic scientists. It depicts the nature of the DNA and act as a person identifier. By knowing the genomic sequences a scientist could easily predict the DNA of the person.
Identification Of Diseases
In this era this technology has become a boon or us in many ways. This concept of DNA testing is booming in the market like anything. It has become the latest rage specially in the health care market. Many companies are there who will promote you the test kits and let you know about the diseases like cardiac problem, any alzimers syndromes, any indication of breast cancer if have any. But here comes the argument many feeble minded people are there who will completely go against it. As the procedures includes blood testing and many people are phobic of syringes, then comes the acceptance of the disease if have any.
Benefits of DNA testing
Many women in our country are facing problem with breast cancer. As they are afraid of testing their blood and DNA. They don’t have the exact DNA marker. This is a big concern for the physicians as well. It is always recommended having a DNA testing for a healthy life. The risk factors get decreased than before and longevity will increase. Myopic thinking should be removed from the society and should learn to accept the truth. DNA testing will also give information about the allergic reaction with various pathogens. The life will be in a proper and better shape.
Detection of criminals
Normal people have a vague idea about this DNA testing which is not at all harmful. Through DNA testing crimes can also be detected. Like in the forensic lab the victim’s body will undergo a scrupulous check up and easily it will be detected about the death of the person. Many criminal lawyers take support from this process which make their job easy to detect the criminal. Though it’s not a good indication for the crime masters. By taking the blood sample or from the foot steps even, of the suspected person it will unveil the truth of the real murder. It can save our nation from crimes.
Detection of pregnancy
Many unrevealed and awful activities like rape case, illegal affairs lead to pregnancy. In many cases the male denied for becoming a father. The lady could easily raise her voice for justice, here
Comes the major role of DNA testing which will easily catch hold of the real father. Sometimes it is not safe for the pregnant woman to have DNA testing as it might cause harm to the fetus. The illegal activities in our society have inflated than before so DNA testing should play a vital role in the society of crimes.The lady will get justice and to deliver her baby.
As a whole it’s a win-win situation because some hospitals testing the DNA of the fetus take place, as an outcome they reveal the gender, which might lead to a death of a baby girl.This is an illegal activity. Some lawyers are also against this. But in this contemporary era one must believe in DNA testing to save our nation.
Source:
http://www.biotechblog.org/entry/dna-testing-good-bad-discuss/
Background:
Currently, the most promising microorganism used for the bio-production of butyric acid is Clostridium tyrobutyricum ATCC 25755T; however, it is unable to use sucrose as a sole carbon source. Consequently, a newly isolated strain, Bacillus sp. SGP1, that was found to produce a levansucrase enzyme, which hydrolyzes sucrose into fructose and glucose, was used in a co-culture with this strain, permitting C. tyrobutyricum ATCC 25755T to ferment sucrose to butyric acid.
Results:
B. sp. SGP1 alone did not show any butyric acid production and the main metabolite produced was lactic acid. This allowed C. tyrobutyricum ATCC 25755T to utilize the monosaccharides resulting from the activity of levansucrase together with the lactic acid produced by B. sp. SGP1 to generate butyric acid, which was the main fermentative product within the co-culture. Furthermore, the final acetic acid concentration in the co-culture was significantly lower when compared with pure C. tyrobutyricum ATCC 25755T cultures grown on glucose. In fed-batch fermentations, the optimum conditions for the production of butyric acid were around pH 5.50 and a temperature of 37[degree sign]C. Under these conditions, the final butyrate concentration was 34.2+/-1.8 g/L with yields of 0.35+/-0.03 g butyrate/g sucrose and maximum productivity of 0.3+/-0.04 g/L/h.
Conclusions:
Using this co-culture, sucrose can be utilized as a carbon source for butyric acid production at a relatively high yield. In addition, this co-culture offers also the benefit of a greater selectivity, with butyric acid constituting 92.8% of the acids when the fermentation was terminated.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/35
Background:
The investigation of structural organisation in lignocellulose materials is important to understand changes in cellulase accessibility and reactivity resulting from hydrothermal deconstruction, to allow development of strategies to maximise bioethanol process efficiencies. To achieve progress, wheat straw lignocellulose and comparative model wood cellulose were characterised following increasing severity of hydrothermal treatment. Powder and fibre wide-angle X-ray diffraction techniques were employed (WAXD), complemented by enzyme kinetic measurements up to high conversion.
Results:
Evidence from WAXD indicated that cellulose fibrils are not perfectly crystalline. A reduction in fibril crystallinity occurred due to hydrothermal treatment, although dimensional and orientational data showed that fibril coherency and alignment were largely retained. The hypothetical inter-fibril spacing created by hydrothermal deconstruction of straw was calculated to be insufficient for complete access by cellulases, although total digestion of cellulose in both treated straw and model pulp was observed. Both treated straw and model pulps were subjected to wet mechanical attrition, which caused separation of smaller fibril aggregates and fragments, significantly increasing enzyme hydrolysis rate. No evidence from WAXD measurements was found for preferential hydrolysis of non-crystalline cellulose at intermediate extent of digestion, for both wood pulp and hydrothermally treated straw.
Conclusions:
The increased efficiency of enzyme digestion of cellulose in the lignocellulosic cell wall following hydrothermal treatment is a consequence of the improved fibril accessibility due to the loss of hemicellulose and disruption of lignin. However, incomplete accessibility of cellulase at the internal surfaces of fibrillar aggregates implies that etching type mechanisms will be important in achieving complete hydrolysis. The reduction in crystalline perfection following hydrothermal treatment may lead to an increase in fibril reactivity, which could amplify the overall improvement in rate of digestion due to accessibility gains. The lack of preferential digestion of non-crystalline cellulose is consistent with the existence of localised conformational disorder, at surfaces and defects, according to proposed semicrystalline fibril models. Cellulases may not interact in a fully selective manner with such disordered environments, so fibril reactivity may be considered as a function of average conformational states.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/33
Background:
A vast number of organisms are known to produce structurally diversified cellulases capable of degrading cellulose, the most abundant biopolymer on earth. The generally accepted paradigm is that the carbohydrate-binding modules (CBMs) of cellulases are required for efficient saccharification of insoluble substrates. Based on sequence data, surprisingly more than 60% of the cellulases identified lack carbohydrate-binding modules or alternative protein structures linked to cellulases (dockerins). This finding poses the question about the role of the CBMs: why would most cellulases lack CBMs, if they are necessary for the efficient hydrolysis of cellulose?
Results:
The advantage of CBMs, which increase the affinity of cellulases to substrates, was found to be diminished by reducing the amount of water in the hydrolytic system, which increases the probability of enzyme-substrate interaction. At low substrate concentration (1% w/w), CBMs were found to be more important in the catalytic performance of the cellobiohydrolases TrCel7A and TrCel6A of Trichoderma reesei as compared to that of the endoglucanases TrCel5A and TrCel7B. Increasing the substrate concentration while maintaining the enzyme-to-substrate ratio enhanced adsorption of TrCel7A, independent of the presence of the CBM. At 20% (w/w) substrate concentration, the hydrolytic performance of cellulases without CBMs caught up with that of cellulases with CBMs. This phenomenon was more noticeable on the lignin-containing pretreated wheat straw as compared to the cellulosic Avicel, presumably due to unproductive adsorption of enzymes to lignin.
Conclusions:
Here we propose that the water content in the natural environments of carbohydrate-degrading organisms might have led to the evolution of various substrate-binding structures. In addition, some well recognized problems of economical saccharification such as unproductive binding of cellulases, which reduces the hydrolysis rate and prevents recycling of enzymes, could be partially overcome by omitting CBMs. This finding could help solve bottlenecks of enzymatic hydrolysis of lignocelluloses and speed up commercialization of second generation bioethanol.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/30
Background:
Cellulose is highly recalcitrant and thus requires a specialized suite of enzymes to solubilize it into fermentable sugars. In C. thermocellum, these extracellular enzymes are present as a highly active multi-component system known as the cellulosome. This study explores the expression of a critical C. thermocellum cellulosomal component in T. saccharolyticum as a step toward creating a thermophilic bacterium capable of consolidated bioprocessing by employing heterologously expressed cellulosomes.
Results:
We developed an inducible promoter system based on the native T. saccharolyticum xynA promoter, which was shown to be induced by xylan and xylose. The promoter was used to express the cellulosomal component cipA*, an engineered form of the wild-type cipA from C. thermocellum. Expression and localization to the supernatant were both verified for CipA*. When a DeltacipA mutant C. thermocellum strain was cultured with a CipA*-expressing T. saccharolyticum strain, hydrolysis and fermentation of 10 grams per liter SigmaCell 101, a highly crystalline cellulose, were observed. This trans-species complementation of a cipA deletion demonstrated the ability for CipA* to assemble a functional cellulosome.
Conclusion:
This study is the first example of an engineered thermophile heterologously expressing a structural component of a cellulosome. To achieve this goal we developed and tested an inducible promoter for controlled expression in T. saccharolyticum as well as a synthetic cipA. In addition, we demonstrate a high degree of hydrolysis (up to 93%) on microcrystalline cellulose.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/32
Background:
Consolidated bioprocessing (CBP) of lignocellulosic biomass to ethanol using thermophilic bacteria provides a promising solution for efficient lignocellulose conversion without the need for additional cellulolytic enzymes. Most studies on the thermophilic CBP concentrate on co-cultivation of the thermophilic cellulolytic bacterium Clostridium thermocellum with non-cellulolytic thermophilic anaerobes at temperatures of 55[degree sign]C-60[degree sign]C.
Results:
We have specifically screened for cellulolytic bacteria growing at temperatures >70[degree sign]C to enable direct conversion of lignocellulosic materials into ethanol. Seven new strains of extremely thermophilic anaerobic cellulolytic bacteria of the genus Caldicellulosiruptor and eight new strains of extremely thermophilic xylanolytic/saccharolytic bacteria of the genus Thermoanaerobacter isolated from environmental samples exhibited fast growth at 72[degree sign]C, extensive lignocellulose degradation and high yield ethanol production on cellulose and pretreated lignocellulosic biomass. Monocultures of Caldicellulosiruptor strains degraded up to 89-97% of the cellulose and hemicellulose polymers in pretreated biomass and produced up to 72 mM ethanol on cellulose without addition of exogenous enzymes. In dual co-cultures of Caldicellulosiruptor strains with Thermoanaerobacter strains the ethanol concentrations rose 2- to 8.2-fold compared to cellulolytic monocultures. A co-culture of Caldicellulosiruptor DIB 087C and Thermoanaerobacter DIB 097X was particularly effective in the conversion of cellulose to ethanol, ethanol comprising 34.8 mol% of the total organic products. In contrast, a co-culture of Caldicellulosiruptor saccharolyticus DSM 8903 and Thermoanaerobacter mathranii subsp. mathranii DSM 11426 produced only low amounts of ethanol.
Conclusions:
The newly discovered Caldicellulosiruptor sp. strain DIB 004C was capable of producing unexpectedly large amounts of ethanol from lignocellulose in fermentors. The established co-cultures of new Caldicellulosiruptor strains with new Thermoanaerobacter strains underline the importance of using specific strain combinations for high ethanol yields. These co-cultures provide an efficient CBP pathway for ethanol production and represent an ideal starting point for development of a highly integrated commercial ethanol production process.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/31
The Indian Express | More funds to flow into Department of Biotechnology's kitty | The ... The Indian Express It's indeed a welcome move that DBT has been awarded higher amount of money this year. However, the budget is disappointing as there was no concrete plan ... |
See original here:
More funds to flow into Department of Biotechnology's kitty | The ... - The Indian Express
On March 9, 2017, the National Academies of Sciences, Engineering, and Medicine (NAS) announced the release (pre-publication version) of a new report:Preparing for Future Products of Biotechnology.Pursuant to the White House Office of Science and Technology Policy's (OSTP) July 2, 2015, memorandum, Modernizing the Regulatory System for Biotechnology Products, NAS was tasked with looking into the future and describing the possible future products of biotechnology that will arise over the next five to ten years, as well as providing some insights that can help shape the capabilities within the agencies as they move forward.
Via an ad hoc committee, the Committee on Future Biotechnology Products and Opportunities to Enhance Capabilities of the Biotechnology Regulatory System, NAS developed this report through several months of gathering and synthesizing information from several sources, including: 74 speakers over the course of three in-person meetings and eight webinars, including one presented by Lynn L. Bergeson; responses to its request for information from a dozen federal agencies; statements solicited from members of the public at its in-person meetings; written comments through the duration of the study; and recent NAS studies related to future products of biotechnology.
The report presents conclusions concerning the future biotechnology products themselves, as well the challenges that federal agencies will face in regulating them, which include:
The bioeconomy is growing rapidly and the U.S. regulatory system needs to provide a balanced approach for consideration of the many competing interests in the face of this expansion;
The profusion of biotechnology products over the next five to ten years has the potential to overwhelm the U.S. regulatory system, which may be exacerbated by a disconnect between research in regulatory science and expected uses of future biotechnology products;
Regulators will face difficult challenges as they grapple with a broad array of new types of bio-technology products -- for example, cosmetics, toys, pets, and office supplies -- that go beyond contained industrial uses and traditional environmental release;
The safe use of new biotechnology products requires rigorous, predictable, and transparent risk-analysis processes whose comprehensiveness, depth, and throughput mirror the scope, scale, complexity, and tempo of future biotechnology applications.
The report provides three recommendations for federal agencies in responding to these challenges, which it states should be taken to enhance the ability of the biotechnology regulatory system to oversee the consumer safety and environmental protection required for future biotechnology products:
The U.S. Environmental Protection Agency (EPA), the U.S. Food and Drug Administration (FDA), the U.S. Department of Agriculture (USDA), and other agencies involved in regulation of future bio-technology products should increase scientific capabilities, tools, expertise, and horizon scanning in key areas of expected growth of biotechnology, including natural, regulatory, and social sciences.
EPA, FDA, and USDA should increase their use of pilot projects to advance understanding and use of ecological risk assessments and benefit analyses for future biotechnology products that are unfamiliar and complex and to prototype new approaches for iterative risk analyses that incorporate external peer review and public participation.
The National Science Foundation, the Department of Defense, the Department of Energy, the National Institute of Standards and Technology, and other agencies that fund bio-technology research with the potential to lead to new biotechnology products should increase their investments in regulatory science and link research and education activities to regulatory-science activities.
The report is well-written and contains an impressive amount of new, relevant, and important information. The Committee participants are to be commended for an important new piece of scholarship in this area.
The reports conclusions are also significant, but not entirely unexpected.For those of us working in this space, we have recognized for years the lack of clarity regarding jurisdictional boundaries, the paucity of government resources, and the urgent need for regulatory clarity and significantly enhanced funding. Unfortunately, given current Trump Administration efforts to diminish government funding for EPA, FDA, and elsewhere, the well-crafted and spot-on recommendations may tragically fall on deaf ears.Shareholders should carefully review the report and work hard to ensure the recommendations are implemented. The consequences of failing to increase scientific capabilities, tools, expertise, and horizon scanning in key areas of expected growth of biotechnology, including natural regulatory, and social sciences -- the number one recommendation in the report -- are too great to ignore.
2017 Bergeson & Campbell, P.C.
Read more:
NAS Issues Report on Preparing for Future Products of ... - The National Law Review
Biotechnology at the Cutting Edge - Jay Keasling
Jay Keasling, Berkeley Lab ALD for Biosciences and CEO of the Joint BioEnergy Institute, appears in a video on biotechnology at the Smithsonian #39;s National Museum of American History. The video is part of en exhibit titled "Science in American Life," which examines the relationship between science, technology, progress and culture through artifacts, historical photographs and multimedia technology.
By: JointBioenergyInst
Read the original post:
Biotechnology at the Cutting Edge - Jay Keasling - Video
Dylan Koski-Budabin - Biotechnology Presentation
Recombinant DNA to produce human proteins
By: Dylan Koski-Budabin
Read the original post:
Dylan Koski-Budabin - Biotechnology Presentation - Video
Background:
Production of biodiesel from non-edible oils is receiving increasing attention. Tung oil, called "China wood oil" is one kind of promising non-edible biodiesel oil in China. To our knowledge, tung oil has not been used to produce biodiesel by enzymatic method. The enzymatic production of biodiesel has been investigated extensively by using Rhizopus oryzae lipase as catalyst. However, the high cost of R. oryzae lipase remains a barrier for its industrial applications. Through different heterologous expression strategies and fermentation techniques, the highest expression level of the lipase from R. oryzae reached 1334 U/mL in Pichia pastoris, which is still not optimistic for industry applications.
Results:
The prosequence of lipases from Rhizopus sp. is very important for the folding and secretion of an active lipase. A chimeric lipase from R. oryzae was constructed by replacing the prosequence with that from the R. chinensis lipase and expressed in P. pastoris. The maximum activity of the chimera reached 4050 U/mL, which was 11 fold higher than that of the parent. The properties of the chimera were studied. The immobilized chimera was used successfully for biodiesel production from tung oil, which achieved higher FAME yield compared with the free chimeric lipase, non-chimeric lipase and mature lipase. By response surface methodology, three variables, water content, methanol to tung oil molar ratio and enzyme dosage were proved to be crucial parameters for biosynthesis of FAME and the FAME yield reached 91.9+/-2.5 % at the optimized conditions by adding 5.66 wt.% of the initial water based on oil weight, 3.88 of methanol to tung oil molar ratio and 13.24 wt.% of enzyme concentration based on oil weight at 40 [degree sign]C.
Conclusions:
This is the first report on improving the expression level of the lipase from R. oryzae by replacing prosequences. The immobilized chimera was used successfully for biodiesel production from tung oil. Using tung oil as non-edible raw material and a chimeric lipase from R. oryzae as an economic catalyst make this study a promising one for biodiesel applications.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/29
ONEIA Presents... Web-I (Waterloo Environmental Biotechnology Inc.)
Find out more about how Web-I uses plants to remove pollutants from the soil. Find out more about them at http://www.waterlooenvironmentalbiotechnology.com.
By: ONEIAmedia
Read the original:
ONEIA Presents... Web-I (Waterloo Environmental Biotechnology Inc.) - Video
Background:
Xylanase is an important component of hemicellulase enzyme system. Since it plays an important role in the hydrolysis of hemicellulose into xylooligosaccharides (XOs), high thermostable xylanase has been the focus of much recent attention as powerful enzyme as well as in the field of biomass utilization.
Results:
A xylanase gene (xyn10A) with 3,474 bp was cloned from the extremely thermophilic bacterium Thermotoga thermarum that encodes a protein containing 1,158 amino acid residues. Based on amino acid sequence homology, hydrophobic cluster and three dimensional structure analyses, it was attested that the xylanase belongs to the glycoside hydrolase (GH) families 10 with five carbohydrate binding domains. When the xylanase gene was cloned and expressed in Escherichia coli BL21 (DE3), the specific enzyme activity of xylanase produced by the recombinant strain was up to 145.8 U mg-1. The xylanase was optimally active at 95[degree sign]C, pH 7.0. In addition, it exhibited high thermostability over broad range of pH 4.0-8.5 and temperature 55-90[degree sign]C upon the addition of 5 mM Ca2+. Confirmed by Ion Chromatography System (ICS) analysis, the end products of the hydrolysis of beechwood xylan were xylose, xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose.
Conclusions:
The xylanase from T. thermarum is one of the hyperthermophilic xylanases that exhibits high thermostability, and thus, is a suitable candidate for generating XOs from cellulosic materials such as agricultural and forestry residues for the uses as prebiotics and precursors for further preparation of furfural and other chemicals.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/26