New UN report outlines the ‘Anatomy of a Genocide’ in Gaza – Mondoweiss

Last week, the UN Special Rapporteur on Occupied Palestinian Territories Francesca Albanese issued a report titled Anatomy of a Genocide, concluding that there are reasonable grounds to believe that the threshold indicating the commission of the crime of genocide has been met.

Such reports are not usually titled, but the title itself depicts the nature of what Albanese describes in her immaculate 25-page report. It not only outlines a textbook case of genocide, with clearly and widely expressed intent by leaders backed up by clear genocidal actions it also describes how Israel has used the language and principles of International Humanitarian Law (IHL, the laws governing warfare) to conceal the act.

Albanese begins with the historical context of the genocide, even rooted in Israeli pre-state colonialist intents, and then charts out how Israel has committed three central facets under the crime of genocide the killing of members of a particular group of people, causing bodily or mental harm, and creating destructive conditions of life. There is also a section concerning intent that chronicles some of the countless, unambiguous, genocidal expressions of Israeli leaders.

Then, midway into the report, and constituting almost half of it (points 55-92, out of 97 points), is a section dedicated to the idea of Humanitarian camouflage: distorting the laws of war to conceal genocidal intent.

This section is subdivided into five central themes:

Albanese demonstrates how the three central principles of IHL distinction, proportionality, and precaution have been subverted by Israel in an attempt to obfuscate its genocidal acts and to provide a legal veil for illegal, indiscriminate acts.

This has obscured one cardinal tenet of IHL, Albanese writes. Indiscriminate attacks, which do not distinguish military targets from protected persons and objects, cannot be proportionate and are always unlawful.

Palestinians have become decivilianized in this way:

A core feature of Israels conduct since 7 October has been the intensification of its de-civilianization of Palestinians, a protected group under the [Genocide] Convention. Israel has used IHL terminology to justify its systematic use of lethal violence against Palestinian civilians as a group and the extensive destruction of life-sustaining infrastructures. Israel has done this by deploying IHL concepts such as human shields, collateral damage, safe zones, evacuations and medical protection in such a permissive manner so as to gut these concepts of their normative content, subverting their protective purpose and ultimately eroding the distinction between civilians and combatants in Israeli actions in Gaza.

It is chilling to think that Israels Defense Ministers claim that we are dealing with human animals and we will act accordingly (October 9), has translated into reality in such a way that humans are reduced to conceptual rubble dehumanized and decivilainized.

The analysis continues to outline Israels subversion of IHL norms, including concepts like human shields, military objectives, and collateral damage.

The history of the general usage of this term as a pretext for indiscriminate attacks is mentioned, particularly in the 2008-2022 aggressions. But on October 7 this rose to a new level:

After 7 October, this macro-characterization of Gazas civilians as a population of human shields has reached unprecedented levels, with Israels top-ranking political and military leaders consistently framing civilians as either Hamas operatives, accomplices, or human shields among whom Hamas is embedded International law does not permit the blanket claim that an opposing force is using the entire population as human shields en bloc The accusation of using human shields has thus become a pretext, justifying the killing of civilians under a cloak of purported legality, whose all-enveloping pervasiveness admits only of genocidal intent.

International law stipulates that attacks must be strictly limited to objects that must offer a definite military advantage. But, Albanese notes, Israel has misused this rule to militarize civilian objects and whatever surrounds them, justifying their indiscriminate destruction. Thus, Gazas civilian population and infrastructure are presented as obstructions positioned amongst, in front of and above targets Israel has characterized the whole territory as a military objective Israel considers any object that has allegedly been or might be used militarily as a legitimate target, so that entire neighbourhoods can be razed or demolished under fictions of legality.

Israel seeks to conceal the widescale targeting of civilians as collateral damage. Invoking the concept of proportionate collateral damage to knowingly shell large numbers of members of the protected group, Israel asserts that when attacks result in more collateral damage than expected, this does not necessarily indicate a violation, since compliance is conduct-oriented, not result-oriented.In other words, Israel says that it doesnt mean to kill many civilians it just occurs despite precautions. However, Albanese writes,

in all attacks launched against residential towers without warnings, extensive civilian harm has been anticipated as the main outcome. The Al-Taj building was full of families at the time of the 31 October strike, which must have been anticipated as certainly killing or injuring all the civilians living there. The fact that so many people were killed was entirely predictable hence at least indirectly intended as is evident from the images that the Israeli military itself published. The attack on the Jabalia refugee camp on 25 October killed at least 126 civilians, including 69 children, and injured a further 280. Israeli military personnel affirmed that the target was one Hamas commander in an underground base.

Israel seems to assess proportionality not only as a question of military advantage but as a question of destroying Hamas politically:

Israels proportionality assessments have flouted legal requirements by defining military advantage, in each attack, in relation to the destruction of the whole Hamas organization both politically and militarily. It is manifestly illegal to declare as a war aim the destruction of the other sides political capacity (particularly in the context of a 56-year military occupation which deprives the occupied population of its right to self-determination) In other words, Israel appears to represent itself as conducting a proportionate genocide.

Albanese writes:

The mass evacuation order of 13 October when 1.1 million Palestinians were ordered to evacuate northern Gaza in 24 hours to Israeli-designated safe zones in the south Instead of increasing safety for civilians, the sheer scale of evacuations amidst an intense bombing campaign, and the haphazardly communicated safe zones system, along with extended communications blackouts, increased levels of panic, forced displacement and mass killing.

When the northern residents were evacuated to the south, Israel illegally categorized the inhabitants of northern Gaza who had remained (including the sick and wounded) as human shields and accomplices of terrorism, thereby turning hundreds of thousands of civilians into legitimate military targets or collateral casualties through impossible-to-follow evacuation orders.

And the safe zones werent safe either:

The erasure of civilian protections in the evacuated
area was combined with indiscriminate targeting of evacuees and inhabitants of the areas designated as safe zones Of the roughly 500 2,000-pound bombs dropped by Israel in the first six weeks of hostilities, 42 percent were deployed in the designated safe zones in southern areas.

Simply put, Albanese summarizes, safe areas were deliberately turned into areas of mass killing.

This has been a tool of ethnic cleansing:

The pattern of killings of civilians who evacuated to the south, in combination with statements of some senior Israelis declaring an intent to forcibly displace Palestinians outside Gaza and replace them with Israeli settlers, lead to reasonably infer that evacuation orders and safe zones have been used as genocidal tools to achieve ethnic cleansing.

This point sends a chill down the spine, as Gazas largest hospital, al-Shifa has now been reduced to burned rubble, with bodies strewn across the area, following Israels recent two-week-long siege.

A final layer of Israels humanitarian camouflage concerns its efforts to provide legal cover for systematic attacks against medical facilities and personnel, causing the progressive collapse of Gazas healthcare sector, writes Albanese.

This is not the first time Israel accused Hamas of using hospitals as Hamas headquarters, but in the current assault, Israel has invoked this legal strategy to justify genocide through the complete destruction of life-sustaining infrastructure.

Israel was widely challenged on its former assault on the al-Shifa hospital in November.

Media reports challenged Israels allegations that Hamas were using hospitals as shields, asserting that there was no evidence to suggest that the rooms connected to the hospital had been used by Hamas; the hospital buildings (contrary to Israeli military 3D images) were found not to be connected to the tunnel network; and there was no evidence that the tunnels were accessible from the hospital wards. In addition, the Israeli army reportedly rearranged weaponry at al-Shifa before news crews visits, raising further suspicions of fabrication after the Israeli army had claimed that a list of terrorists it had found in another Gaza hospitalthe Al Rantisiturned out to be a calendar of the days of the week in Arabic.

The level of the Israeli propaganda would be something to laugh about (and was widely mocked) if it werent so deadly serious. Even if one were to take these repeated allegations at face value, Israel behaved illegally:

Whether or not Israels accusations of hospital shielding at al-Shifa were true but still remain to be proven , the civilians in the hospitals should have been protected and not subjected to siege and military attack.

This lack of protection for civilians was itself genocidal:

That the intent behind Israels humanitarian camouflage in this instance can only be characterized as genocidal is clear for two reasons. First, Israel was aware of the large-scale destruction of the healthcare system since the World Health Organization had reported in mid-November that a public health catastrophe was developing in Gaza, with 26 of 35 hospitals no longer operational due to Israels bombing and siege. Second, Israel knew that its military operation was resulting in a significant number of wounded. Physical trauma constitutes the most predominant cause of excess mortality in Gaza. It was predictable that forcibly suspending services at the largest hospital in Gaza would seriously harm the prospects for survival of the injured, the chronically ill and newborn babies in incubators. Therefore, by targeting al-Shifa Hospital, Israel knowingly condemned thousands of sick and displaced people to preventable suffering and death.

The overwhelming nature and scale of Israels assault on Gaza and the destructive conditions of life it has inflicted reveal an intent to physically destroy Palestinians as a group, Albanese concludes.

Israel has sought to conceal its eliminationist conduct of hostilities sanctioning the commission of international crimes as IHL-abiding. Distorting IHL customary rules, including distinction, proportionality and precautions, Israel has de facto treated an entire protected group and its life-sustaining infrastructure as terrorist or terrorist-supporting, thus transforming everything and everyone into either a target or collateral damage, hence killable or destroyable.

This goes back to the first Nakba of 1948:

Israels genocide on the Palestinians in Gaza is an escalatory stage of a long-standing settler colonial process of erasure. For over seven decades this process has suffocated the Palestinian people as a group demographically, culturally, economically and politically , seeking to displace it and expropriate and control its land and resources. The ongoing Nakba must be stopped and remedied once and for all. This is an imperative owed to the victims of this highly preventable tragedy, and to future generations in that land.

The last two points (96-97) of the report are about what we the international community can, and indeed must do, to avert this genocide.

The Special Rapporteur urges member states to enforce the prohibition of genocide in accordance with their non-derogable obligations. Israel and those states that have been complicit in what can be reasonably concluded to constitute genocide must be held accountable and deliver reparations commensurate with the destruction, death and harm inflicted on the Palestinian people.

The means at our disposal:

Finally, Albanese calls on the Office of the High Commissioner for Human Rights to enhance its efforts to end the current atrocities in Gaza, including by promoting and accurately applying International Law, notably the Genocide Convention, in the context of the oPt as a whole. That is, to see the genocide of Gaza not merely as a local matter, but as a genocidal assault on all Palestinians, also beyond Gazas geographical confines.

Israels initial, knee-jerk response was to describe the report as an obscene inversion of reality. The problem is, that Albanese has used about half of her meticulous report to document how it is precisely Israel that is inverting reality through the mentioned twisting of IHL terms to justify and continue its genocide.

The U.S., for its part, chose to avoid the subject by shooting the messenger hiding behind regurgitated, false allegations of Albanese as an antisemite as State Department Spokesperson Matthew Miller did in response to a question on the matter:

We have for a longstanding period of time opposed the mandate of this Special Rapporteur, which we believe is not productive. And when it comes to the individual that holds that position, I cant help but note a history of antisemitic comments that she has made, that were reported.

But many other countries, particularly Arab ones as well as other global south countries, rallied behind Albanese and affirmed the seriousness of her report. At the UN presentation of the report last week, Israel and the U.S. boycotted the event. The EU, for its part, called for proper and independent investigations on all allegations, alas recognizing Israels right to self-defense.

Self-defense, it bears mention, is no excuse for genocide, and the reason why one purportedly goes to war has no bearing on how one conducts it. So the EU is avoiding the issue, in a perhaps less
visible manner than Israel and the U.S.

Israel has destroyed Gaza, said the Special Rapporteur. Its not incidental, its not a natural disaster its genocide. Now its only down to those who accept this awful truth and those who hide from it.

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New UN report outlines the 'Anatomy of a Genocide' in Gaza - Mondoweiss

Grey’s Anatomy: Season 20, Episode 3, "Walk on the Ocean," Recap & Spoilers – CBR – Comic Book Resources

The following contains spoilers from Grey's Anatomy, Season 20, Episode 3, "Walk on the Ocean," which aired Thursday, March 28 on ABC.

Amelia Shepherd and Meredith Grey are working hard to get funding for their Alzheimer's research when "Walk on the Ocean" begins. In a conference room in Seattle, Amelia is presenting new research that suggests that Alzheimer's, like Parkinson's, may be connected to changes in the gut's microbiome. The group seemed interested and even wanted to make sure they met Meredith before suggesting supporting the research to their board, but things fell apart as soon as they asked if they should set a meeting with Dr. Catherine Fox to discuss the next steps. Catherine can't know Meredith is doing this research, or she'll lose her job with the Fox Foundation.

At Grey-Sloan Memorial, Mika Yasuda comes barreling into the parking lot in her van, and Lucas Adams comes running up behind her, complaining that she'd nearly hit him on her way in. Their banter is cut off when Simone Griffith comes around the side of the van -- she and Adams are still in an awkward place after she tried to break up with him, and he accused her of being selfish at the end of Season 20, Episode 2, "Keep the Family Close." After Griffith heads inside, Yasuda calls a momentary truce. She's still mad at Adams for the interns being banned from the OR, but she knows he just got dumped and wants to know if he's okay. Adams tells her that he didn't get dumped and heads into the hospital as Yasuda yells, "You're making it very hard to be Team Skywalker!"

Meredith and Amelia are also pulling into the parking lot, and Meredith says she's going to wait in the car during future funding meetings. "Everyone knows my affiliation with Catherine and somebody will talk," she says, before telling Amelia she'll be making more calls from the lab before her flight leaves. Amelia sees a parking spot and pulls around a car with its turn single on and into the spot. Meredith thinks that the person in the car is waiting, but Amelia sees her on the phone. Seconds later, the woman driving the car honks at Amelia and tells her she has been waiting for the spot. "You were on your phone," Amelia says, not at all sorry.

Inside, Richard Webber, Winston Ndugu, and Miranda Bailey are updating the surgical board. Bailey gives Ndugu the opportunity to say whatever he wants about the way she's handling the interns. "Maybe get all that negativity out now instead of sprinkling it throughout my day like you've been doing," she says. Ndugu promises her there is no negativity today as he is headed to Chicago for a vacation with Maggie Pierce, his wife, who no longer lives in Seattle. He does have an idea about the interns he'll discuss with her when he gets back, though.

Levi Schmitt, the co-Chief Resident, is sending the interns off to their assignments for the day. Jules Millin and Griffith head down to the pit while Benson Kwan is with Monica Beltran, the new Peds attending. Yasuda gives Kwan a hard time about "babysitting," but Kwan doesn't care. Beltran was on the team that pioneered single port surgery, so he's excited. Yasuda and Adams are headed to the ICU together. Yasuda offers to trade Millin so that each of them can potentially get the procedures done, but they still need to fill out their procedure logs, but Schmitt stops them. "No trading, negotiating, or complaining," Schmitt says. "Unless one of you picked up a vanilla latte on the way?" Sorry, Schmitt, they did not.

As the interns head to their respective departments, Schmitt spots a ghost from the past. Nico Kim, Schmitt's ex, who used to be an orthopedic surgeon at Grey-Sloan and took a job as the team doctor for the Seattle Mariners, is being greeted by Jo Wilson. Schmitt is shocked to see him.

Up in Peds, Kwan is with a young girl and her mother when Shepherd walks in, followed immediately by Beltran -- who happens to be the woman Shepherd stole a parking spot from. The tension is there immediately, though they do their best to hide it from the patient and her mother. Malan, eight years old, has congenital hydrocephalus and has had multiple shunts in her brain to control the build-up of fluid. Her mother, Maria-Flor, brought her in because "Malan's not acting like herself." Kwan clearly doesn't take the mother's concern very seriously, and Beltran assumes that Shepherd won't either, immediately cutting her off when she says she doesn't see anything wrong with Malan. "But that doesn't mean anything," Beltran says, and Shepherd shakes her head. "Well, it means I wasn't finished with my sentence. Maria-Flor, I'd like to take her for some scans." Maria-Flor is worried because the four previous shunts malfunctioned, and she doesn't want Malan to have another surgery. Shepherd assures her that they're going to take it one step at a time and asks Kwan to get Malan set up in radiology.

Down in the pit, a big trauma is arriving. Owen Hunt says that he needs everyone, including Ndugu, even if he can only stay for a couple of hours. The trauma arriving is serious, but it's also physically big. Nate Ardilla, 34, is pinned inside his watercraft, which was hit by a container ship. The jaws of life are on their way to get Nate out of the "self-propelled floating bubble," which he made himself, but Hunt and Ndugu have to keep him stable until then.

In the ICU, Yasuda and Adams are being given their assignment from Bailey. She wants them to spend the day monitoring Dorian Cardenas, the gunshot victim from "Keep the Family Close," as she'll be spending the day in the OR. She tells them what to watch for and to only page her if certain things are happening. Adams seems annoyed that they'll be spending the day monitoring one guy and pushes back, reminding her that they're also supposed to be doing procedures for their procedure log. Yasuda, who always seems to be one step ahead of the other interns, cuts him off and tells Bailey they are good, hitting Adams in the upper arm as Bailey leaves. "Don't be a smartass to the person who holds all the cards!" she says.

Back outside, Nate tells everyone that he had hoped to make it this time, as it was his fourth try. Ndugu asks if he was trying to get to Bainbridge Island, but no, he was headed for Hong Kong. He's trying to outdo a man named Rich Doyle, the tech billionaire who set a solo record rowing from San Francisco to Hawai'i -- and is also the ex-boyfriend of Nate's wife. He tells the doctors that his wife would say it's a non-issue, but he knows she thinks about Rich. As Atticus "Link" Lincoln, Hunt, and Ndugu share thoughts about what he might have injured and what will need to happen first, Nate starts to panic and tries to get out. Hunt is able to keep him from moving, but he's still scared. He asks if someone can call his wife.

On the other side of the hospital, Wilson is walking through the ambulance bay when Schmitt comes up behind her, asking if she hates him. She wonders why he would ask and he says that she should alert him when certain people are coming in to the hospital. She thinks he's upset because he hasn't gotten to see the guy in his watercraft, but then realizes he might not be and asks him what he's talking about. "Nico," he says, to which she replies, "Who?" He tells her again, though she was likely trying to get out of answering. She says she can't tell him, and he asks if it's about him, but she can't tell him anything because of HIPAA. That's when Schmitt realizes that Wilson is wearing black scrubs. "Why does Nico need an OB-GYN, Jo?!" Wilson tells him goodbye and walks onto the elevator. Schmitt is panicking.

Just beyond the coffee cart outside, Meredith runs into Teddy as they're both walking into the hospital. Teddy is there for physical therapy after her heart surgery and is frustrated that no one will tell her anything while she's recovering. Ndugu has locked her out of her charts, Hunt is keeping things about their kids from her, and Webber won't let her do any admin. She invites Meredith to come to physical therapy with her so that they can catch up, and Meredith isn't going to go until she sees Catherine Fox standing on the stairs. Not wanting to run into her boss, Meredith joins Teddy on the elevator.

While Malan is being scanned, Kwan is asking Beltran and Shepherd about where they were before Grey Sloan and shares some of his own educational background, which he doesn't get a great response to. Then Malan's scans are back, and Shepherd says they'll have to replace the shunt. Beltran reminds her that it's not working because Malan is an active kid who is still growing, but Shepherd says it's the standard of care and that they'll just have to talk to the mom about Malan's physical activity. Beltran suggests an endoscopic ventriculostomy, which Shepherd also turns down for being near impossible in Malan's brain. Beltran tells her to take a minute to think about it, and when Shepherd counters with the list of things on her plate, Beltran throws back her own list, which is all on kids. Beltran is sure Shepherd can look into another option, and she'll pass that information on to Malan's mom. Shepherd is annoyed, but she'll do it. She's not going to leave the kid hanging.

In the ER, Millin and Griffith are gathering supplies to take outside while Griffith is calling Nate's wife and leaving her a message. While they collect things, Millin tries to strike up a conversation with Griffith about Adams, and Griffith counters by asking about Kwan, so they both stop asking questions. And up in the ICU, Yasuda and Adams are sitting with Dorian. Yasuda asks Adams about his family, and he tells her they don't have to talk. She pushes a little more, and Adams stands up to leave. Yasuda reminds him about the explicit instructions Bailey gave them, and he says that they're also supposed to be doing "about a zillion procedures if we ever want to set foot in the OR again," so he's going to go do that. Yasuda tells him that she's not leaving Dorian. "Well, I'm not letting Kwan get back in the OR before I do," Adams says, opening the door to Dorian's room. "So, uh, page me if he codes."

Outside, Hunt asks Ndugu if he needs to head to the airport, but after looking at his phone, Ndugu would rather just get the guy out. Hunt gets ready to move the bar off of Nate, who stops them and asks them to explain what will happen. Hunt tells him he's not totally sure but that their whole team is there to help. Then he asks, "What if I die?" He begins another question, "What if I die without ever getting...," but Ndugu cuts him off. "You're never getting to Hong Kong in a bubble," Ndugu snaps. "All you succeeded in doing is taking away resources from the Coast Guard when they rescued you. Not to mention the paramedics. Not everyone gets to go where they want to go." Something is clearly up with Ndugu, who looks only slightly apologetic when Nate tells him that he was going to say "without ever getting to see my wife again." She's not there, but Hunt says they can wait a few minutes and tells Griffith to try her again.

Kwan is at a computer, working on a chart, when Maria-Flor comes out of Malan's room to ask for headphones because the hospital noises bother Malan. She says that the radiology department sometimes has the ones that block out the noise, but Kwan quickly dismisses her and tells her that they don't loan them out. Beltran is behind him and jumps in immediately, assuring Maria-Flor that they can find them. When Maria-Flor goes back into Malan's room, Kwan tries to reiterate that Radiology doesn't loan them out, and when Beltran tells him to ask nicely, he tells her that won't help. "That woman's daughter is going through something terrifying. If she thinks headphones will help, then get her headphones," Beltran says before asking him to get an update from Shepherd.

Shepherd hasn't yet figured out what they're going to do when she is found by Webber, who asks what is bothering her. Shepherd begins talking about Beltran, and Webber pushes back. "Are you irritated with the suggestion or with the suggester?" he asks, noting that Beltran is known for being a boundary pusherkind of like Shepherd herself.

In the hospital waiting area, Schmitt is stopped by Nico, who wants to talk to him. They are interrupted by Jason, Nico's partner, who has brought back snacks from the vending machine. The snacks aren't for Nico, though; they're for their surrogate, who is giving them a son. Schmitt introduces himself, expecting some kind of recognition to cross Jason's face, but there is none. Nico obviously hasn't talked about him. To make matters worse, as they head back to their surrogate, Jason calls him Levon.

Teddy and Meredith are in the physical therapy room, and Teddy is telling Meredith about how things are going at home. Hunt's mother is helpful, sort of, but not in the way Teddy would like. Meredith reminds her about what Teddy said to Meredith during COVID, that she should get some rest and ask for help when she needs it. Teddy asks about Boston, but before Meredith can answer, something happens to Teddy's foot, and she falls, first onto the treadmill and then the floor.

Everyone outside is getting ready to transport Nate. They likely won't have much time to get him to the OR, so Link and some other members of the team are already scrubbing in. Nate's wife still hasn't arrived, but they need to take care of him, so Griffith offers to be the keeper of whatever he wants to say to his wife. "I love her. That's all that matters," Nate says. "I love her so much. Everything I do is for her. All of this was for her. I know she married me and not Rich, but I want her to be proud of me. Tell her that I know she has doubts, and I don't want her to spend another second of her life questioning her choosing me. Tell her that I'll never give up trying to do right by her."

The moment the metal is pulled away from Nate, he begins bleeding out. Hunt says they'll need to intubate, and they'll need a clear path to the OR, but Ndugu is sure he won't make it to the OR. He wants to slow the bleeding first, buying them time to transport him. Hunt begins to intubate as Ndugu gets gloved up and begins working on the wound.

Up in the NICU, Schmitt is sharing his frustration that Jason didn't know him with Wilson. She asks him if he wants to get back together with Nico, and Schmitt tells her that he isn't sure he knows Nico anymore because he had never talked about kids when they were together, and now he's having a baby with this guy. Schmitt tells Wilson that he doesn't want to get back together, and she says that he's just upset because Nico's life has moved further along than his. Schmitt pushes back, but Wilson reminds him that he has talked about dating and talked about applying to Peds but has not actually done either of those things. Wilson tells him that if he wants to win, he has to get in the game.

Shepherd has figured out what to do for Malan, and is glad when Beltran and Kwan finally join her to talk about it, even though they were waiting on her. Beltran asks Kwan a question about why part of Malan's brain is a problem for the procedure they want to do, and when he starts talking about it like a doctor, she makes him start over and answer like he's talking to a parent. Shepherd tells th
em that she hasn't found an alternative route, but she thinks they can combine the computer system they're looking at with "the intraoperative imaging technology that we use for mapping brain tumors" for ultra precision in the OR. She herself can't build the technology, but Grey-Sloan's tech team already has it working and Shepherd is willing to try if the Mom is okay with it. Shepherd asks Beltran what she's thinking, and Beltran says, "I'm thinking you're much more effective when you take your time."

On the OR floor, Millin is clearing the hallways as Hunt, Ndugu, and Griffith rush Nate to the OR. Hunt sends Griffith to find Nate's wife. There's no time for Ndugu to scrub, so he tells one of the nurses to gown and glove him so they can begin surgery. As Millin and Griffith are shut out of the OR, Griffith tries calling his wife again.

In the ICU, Yasuda is reading out loud to Dorian when the machines start beeping. At first, it's just one, so she thinks it's just a twisted chord, but then more of them start, so she calls for help.

Meredith is helping Teddy to a patient room because she sprained her ankle on the treadmill, and, much to Meredith's dismay, Catherine Fox pops into the room. When she asks if Meredith is there for a consult, Meredith chokes and Teddy steps in, saying that she asked Meredith for a second opinion on a patient and then asks Meredith about the "thing with the house." Meredith says something about insurance issues, and Catherine buys it, telling Meredith that it's nice to have her there and that she can't wait for the update on Meredith's research in the next month. Meredith tells Teddy that her ankle needs to be X-rayed and Teddy tells her that she wants to hear why Meredith is actually in Seattle.

While Griffith and Millin are watching Ndugu and Hunt perform surgery on Nate, Adams walks in. The tension is thick, right up until Adams gets a page. Millin tells Griffith, who told her that she and Adams were "just roommates," that she doesn't have that much tension with her roommate, to which Griffith responds, "Your roommate is a hundred."

Nico finds Schmitt, and they talk about Nico's surrogate. Schmitt asks if they've picked a name, and Nico jokes that it's "Levon" before apologizing for Jason calling Schmitt "Levon." They talk about how Nico and Jason met, and Nico reveals that Jason was already interviewing surrogates when they started dating. He tells Schmitt that he freaked out at first but eventually realized that it was scarier to consider life without Jason than life with a kid, and Schmitt tells him that he's happy for him.

While Meredith wraps Teddy's ankle, she and Teddy talk about Meredith's secret research with Amelia. Meredith reveals that it's hard to do secret research because, as soon as research gets funded, people want to talk about it, and she can't. Teddy tells her that it's bold and exciting and that she wouldn't talk about it -- and then offers her money from Grey-Sloan's discretionary fund to help them get started. Meredith asks about Catherine since Grey-Sloan is a Fox Foundation hospital, and Teddy tells her that she and Amelia just have to get far enough that Catherine can't deny the value of the research and that she's willing to take the risk on them.

Shepherd and Beltran get ready to start surgery, and when Kwan asks to observe, Beltran tells him that he needs to sit with Malan's mother. Shepherd says that it's a rare surgery, so maybe they should let him watch, but Beltran doesn't want him to, and he's on her service.

In Dorian's room, Yasuda is trying to figure out what is wrong when Adams races in. Yasuda begins talking through it with him and pretty quickly determines that it's Abdominal Compartment Syndrome and races to get a cut-down tray. Shortly after she leaves, Bailey shows up. Adams repeats what Yasuda said to him, and Bailey says they need to open him up right there instead of getting an OR. Yasuda returns with the cut-down tray and tries to explain where she was, but Bailey doesn't let her and gives Adams the opportunity to gown and glove. He looks like he's unsure about what to do for a moment, but he takes the gown and gloves anyway, and Yasuda is devastated.

While Bailey and Adams are working on Dorian, Nate starts to bleed out in the OR, and Shepherd and Beltran begin their procedure. Kwan is in the chapel with Malan's mother, who is praying for her daughter.

Ndugu, Hunt, and Link are unable to save Nate. Shepherd and Beltran go to the chapel to tell Malan's mother that the procedure went well. Kwan escorts her to Malan, but not before she tells Shepherd and Beltran that they make a great team. After the mother leaves, Shepherd says, "That was pretty great," and turns to talk to Beltran, only to find that she has already left the chapel.

Nate's wife has arrived in the waiting room, and Hunt, Ndugu, and Griffith have to tell her that Nate didn't make it out of surgery. She tells them they must be wrong because "he always comes home." She believes that it's her fault because she didn't convince him that all that mattered was them, that Rich Doyle didn't matter. Griffith kneels down next to her. "He knew," Griffith says. "He knew that you loved him. He told me. He said the floating to Hong Kong and the bubble -- none of that was about you. He wanted to prove to himself he could do it. And he told me, if he didn't make it, to tell you that he loved you so much. It's no one's fault. He loved you. You loved him. That's what matters." Ndugu looks on as Griffith speaks to the wife, his eyes beginning to tear up.

In Peds, Kwan brings progress notes back to Beltran, who tells him that she needs another post-op check on a specific patient. He says okay, and then he tells her that he went to Yale for almost a full year before his mom got sick. He moved home to take care of her and then ended up at different schools. He knows he doesn't have a fancy pedigree, but he's a quick study and capable of more than hand-holding a patient's mom. Beltran tells him that being assigned to sit with Malan's mother wasn't punitive because of where he was educated but because of how he interacted with Malan's mother throughout the day. In Peds, patients and their families matter.

Outside the hospital, as the city hauls Nate's watercraft away, Hunt stops to talk to Ndugu. Ndugu finally admits that Maggie canceled on him for Chicago, and he took it out on a patient. It isn't the first time she has canceled on him recently, and he's unsure when to stop trying. Hunt asks him to think about why he's trying, which might tell him what he needs to know. Before the end of the episode, Ndugu takes off his ring.

In the intern locker room, Yasuda finally yells at Adams for taking the ex-lap. It was her diagnosis. Adams asks if there is a rule that the person who finds the diagnosis does the procedure, and Kwan tells him no, but Yasuda tells him to shut up. She reminds him that she was trying to be a good friend to him, and he lied to Bailey, to which he replies that he didn't say anything. She tells him that was the point. He could have told Bailey where she was or that she had stayed with Dorian all day while he had been looking for procedures. Griffith comes to his defense and tells Yasuda to give him a chance to explain, but Yasuda isn't having it. She's done. It's his fault they can't be in the OR, and Yasuda is shocked Griffith is defending him.

"Stop. Just stop," Griffith says, standing up. "I'm tired of the blame and the finger pointing. It is all our faults. I was there, too. So were you. Kwan didn't do us any favors. We all screwed up. There's nothing we can do except put i
t behind us and move on. We are all trying. Why can't you?" Griffith leaves, and Yasuda tells Adams that she doesn't want to be his friend anymore and that he can find another way home. Adams storms out of the locker room, and Kwan, Millin, and Yasuda agree to go for a drink.

Elsewhere, Schmitt stops Beltran to introduce himself and talk to her about Peds. Yasuda is at the bar, and Millin finds herself watching Kwan flirt with another woman. Meredith finds Amelia in the lab, and Amelia wants to keep going on research. Meredith tells her about the money from Teddy.

At the intern house, Griffith tries to talk to Adams. When she knocks on his door, there is no response, even though the light is on, so she opens the door. The room has been cleared out. Adams is gone.

Grey's Anatomy airs Thursdays at 9/8c on ABC.

A drama centered on the personal and professional lives of five surgical interns and their supervisors.

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Grey's Anatomy: Season 20, Episode 3, "Walk on the Ocean," Recap & Spoilers - CBR - Comic Book Resources

Grey’s Anatomy Was Renewed For Season 21, And The Responses On Social Are Wild – CinemaBlend

Well, its official, Greys Anatomy is getting a 21st season. The show about the surgeons of Grey-Sloan Memorial has been gracing our screens for two decades, and now theyll officially be returning to the 2024 TV schedule with another season, extending its run as the longest-running primetime medical drama. Now, considering this series has been on for so long that its of legal drinking age, fans are having a ball on the internet reacting to the news, and their responses are truly wild.

The wild fan reactions about Greys Anatomy getting a 21st season really run the gamut. From happy to angry, folks are feeling all kinds of ways, however, a common denominator among many of these reactions is chaos. For example, @jay_kt2s feelings about the news are hilariously dramatic as they posted:

I am calling on President Biden to bring this show to an end. It is a national security imperative.

This viewer, @adrewscomet, had similar feelings as they posted:

someone sell that hospital

Well, I hate to break it to this person, but this hospital has faced this issue before, and they came out on top. So, I dont think thats going to happen. Also, while Station 19 was canceled, and some were worried Greys would end too, this news confirms that Grey Sloan Memorial isnt going anywhere anytime soon.

Other fans had some great jokes about the renewal and the idea of Greys Anatomy going on forever. Take @jomaticaaas reaction for example:

they must be holograms because how are any of those characters still alive

Thinking along the same lines, @GreysAnatomyHD decided to put their Photoshop skills to work to imagine what Meredith Grey would look like come Season 50:

It currently takes a lot of work to catch up to Greys Anatomy, just imagine trying to get up to date during Season 50. Thatd be wild, and it would take forever.

Your Daily Blend of Entertainment News

Along the same lines, one popular sentiment revolved around the fact that Greys Anatomy might live longer than all of us. @DannyWxo put it best as they posted:

Greys Anatomy is going to outlive us all.

It truly will, and you know who else will also outlive and outwork us all? Richard Webber. Some fans of the show are getting a kick out of the fact that he said he was going to retire in the early seasons of Greys Anatomy. Considering he was thinking about that 20 years ago, that makes this reaction from @buggirl29 even funnier:

Personally, Im thrilled that Richard Webber, Miranda Bailey and co. will get to keep working. While the cast of Greys Anatomy is consistently cycling in and out, the series has become a constant in many peoples livesheck its been on for the majority of mine (I was 6 years old when Season 1 premiered), so its hard to imagine television without it.

From seeing more relationships flourish, like Lucas and Ameilia and Link and Jo, to following the new interns as they grow into residents and possible attendings, Greys Anatomy has a lot of stories left to tell. So, I for one, am stoked that Season 21 is coming.

If you are feeling up to the challenge, you can stream the first 20 Seasons of Greys with a Hulu subscription, and you can catch new episodes on ABC every Thursday at 9 p.m. ET, that way youll be ready to rock when Season 21 premieres next fall.

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Grey's Anatomy Was Renewed For Season 21, And The Responses On Social Are Wild - CinemaBlend

Grey’s Anatomy and 9-1-1 renewed by ABC – Roster Con

Grey Sloan Memorial Hospital will remain on ABC for at least another year. The network announced that Greys Anatomy has been renewed for a 21st season, continuing to make history on American television.

In a press release, series creator Shonda Rhimes thanked Greys Anatomy fans for their loyalty and love, which has allowed the show to reach this historic number of seasons. She added: Meg Marinis storytelling is a gift that continues to keep the show vibrant, compelling and alive, and I cant wait to see what she has in store for next season.

According to Deadline, Greys Anatomy Season 21 could have 18 episodes. The cast of the new season is not yet known, as many of their contracts expire in the coming months, according to the American media outlet.

Cancelled by FOX at the end of its sixth season, 9-1-1 was quickly picked up by ABC, which saw it as an excellent addition to its existing lineup. The U.S. network doesnt seem to regret its decision, as it has just made it official that the series will return for a Season 8 in 2024-2025.

This announcement comes just a few weeks after the launch of Season 7 on ABC. The arrival of 9-1-1 on the American network has been noticed as it has improved its average audience compared to the previous season (5.29 million vs. 4.7 million). In addition, the first episode of season 7 attracted 11.76 million viewers on ABC and the Groups various platforms.

9-1-1 will pass a symbolic milestone in its seventh season, currently airing, when the series reaches 100 episodes.

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Grey's Anatomy and 9-1-1 renewed by ABC - Roster Con

Grey’s Anatomy renewed for season 21 – PopBuzz

3 April 2024, 10:53

Meredith says goodbye in Greys Anatomys season 19 episode 7 trailer

Season 21 will coincide with Grey's Anatomy's 20th anniversary.

It's official Grey's Anatomy has been renewed for season 21 (!), lengthening its lead as the longest running US medical drama, and the longest running primetime series in ABC history.

The renewal now means that season 21 will be airing when the show's 20th anniversary comes around in March 2025. There's been no details shared just yet about what the writers are planning for that landmark anniversary but fans will no doubt be treated to some pretty iconic surprises.

According to Deadline, season 21 will consist of 18 episodes. The strike-impacted season 20 will end with 10 episodes, making it the shortest season since the show began in 2005. (Season 1 consists of 9 episodes.)

READ MORE: Here's how Meredith Grey leaves Grey's Anatomy in Ellen Pompeo's last episode

Responding to the news of the early renewal, creator and executive producer Shonda Rhimes wrote in a post: "Greys was something I made up 20+ years ago, and I am so incredibly proud that its been picked up for its 21st season. This honestly could not be possible without you guys..caring about the stories I tell, the talented cast, writers, and crew. Wow."

In another statement, issued to The Hollywood Reporter, Shonda added: "The loyalty and love of Greys Anatomy fans has propelled us into a historic 21st season, and I could not be more grateful. [Showrunner] Meg Marinis storytelling is a gift that continues to keep the show vibrant, compelling and alive, and I cant wait to see what she has in store for next season."

In early 2023, Ellen Pompeo officially ended her status as a series regular and the show's lead, and Meredith moved away from Seattle to Boston with her children. (She still pops up every now and then, provides the iconic voiceovers that open and close the episodes, and still serves as an executive producer.)

The early renewal is a clear testament to the show's staying power and popularity, even without Meredith and Ellen at the helm. Twenty years on, Grey's still attracts thousands and thousands of new viewers thanks to streaming and viral clips on TikTok.

According to various reports, the contracts for almost all of the show's leads are up for renegotiation at the end of season 20, so it's unclear who will return for season 21 just yet.

Joe Keery Gets Deep In 'My Life In 20 Questions' | Djo

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Grey's Anatomy renewed for season 21 - PopBuzz

‘Grey’s Anatomy’ Fans Get a Big Update About the Future of the Show – MDJOnline.com

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'Grey's Anatomy' Fans Get a Big Update About the Future of the Show - MDJOnline.com

‘Grey’s Anatomy’ is coming back for its 21st season – Yahoo! Voices

"Grey's Anatomy" will soon be back in action.

MORE: Shonda Rhimes reveals daughter started watching 'Grey's Anatomy': 'My brain is breaking'

The ABC hit show, created by Shonda Rhimes, is coming back for a 21st season after ABC renewed the popular medical drama, "Good Morning America" confirmed Tuesday.

"The loyalty and love of 'Grey's Anatomy' fans has propelled us into a historic 21st season, and I could not be more grateful," read a statement from Rhimes, provided to ABC News.

"Meg Marinis' storytelling is a gift that continues to keep the show vibrant, compelling and alive, and I can't wait to see what she has in store for next season," Rhimes added.

MORE:Patrick Dempsey talks 'greatest gift' he got from 'Grey's Anatomy'

The announcement extends "Grey's Anatomy" run as the longest running primetime medical drama in TV history, a title the show has held since 2019. The show also claims the run for the longest running primetime series in ABC history.

'Greys Anatomy' is coming back for its 21st season originally appeared on goodmorningamerica.com

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'Grey's Anatomy' is coming back for its 21st season - Yahoo! Voices

‘Grey’s Anatomy’s Jessica Capshaw Celebrates Her Guest Return to the Series – Collider

The Big Picture

Fans of Grey's Anatomy already knew from the Season 20 trailer that Jessica Capshaw would make an anticipated return as Arizona Robbins after a six-season absence. Now, thanks to The Wrap, we get to hear from Capshaw herself what it felt like to walk across the Grey Sloan Memorial Hospital corridors and catwalk once again, as well as what she can reveal about the current season.

During the interview, Capshaw revealed that even though she's been away from quite a while, stepping back into Arizona's shoes was a "seamless experience" and that it "was just fun." She celebrated the fact that, even though she's only back as a guest star for one episode, she got to hang out with old friends and that she screamed with excitement at the sight of several of them. But she also revealed what's the point of Arizona returning now, so late into the game:

The mission was very clear for Arizona to come back at this time where everyones a little bit world-weary and missing the magic and joy of healing and bring back some of that joy and levity. Hearing the reactions from viewers feeling so excited to reconnect with her made my heart grow a size for sure.

All the way back in Season 5 when Arizona was introduced, the character was a source of constant joy among doctors, and this was helped by the fact that she was a Pediatrics surgeon who worked with kids all day long. Capshaw also revealed to The Wrap that her return was orchestrated by new showrunner Meg Marinis who might have planned all kinds of surprises for the show's landmark 20th run. Even though Marinis only now has taken over showrunning duties, she's been a writer on the series for 18 years.

Recently, Marinis also broke down at what capacity fans can expect to see title character Meredith Grey (Ellen Pompeo) back in Season 20. Pompeo officially left the series in 2022, but she still serves as narrator for most of the episodes. To The Wrap, Capshaw ended her interview by revealing that, just like fans, she isn't oblivious to the several changes in character dynamics that happened across the show's run. Capshaw stated that she has "tremendous and deep respect for all the changes" that the Grey's Anatomy team did throughout the seasons, and that she feels like the medical series is a house where there's room for everyone and having the best time while there is mandatory.

ABC airs new episodes of Grey's Anatomy on Thursday nights. You can also stream new episodes on Hulu.

A drama centered on the personal and professional lives of five surgical interns and their supervisors.

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'Grey's Anatomy's Jessica Capshaw Celebrates Her Guest Return to the Series - Collider

Greys Anatomy Renewed for Season 21 By ABC! | ABC, Greys Anatomy – Just Jared

Greys Anatomy has been officially renewed for season 21 by ABC!

Shonda Rhimes, the shows creator, released a statement to THR sharing, The loyalty and love of Greys Anatomy fans has propelled us into a historic 21st season, and I could not be more grateful. [Showrunner] Meg Marinis storytelling is a gift that continues to keep the show vibrant, compelling and alive, and I cant wait to see what she has in store for next season.

The series is the longest-running medical TV drama in history.

Keep reading to find out more

THR is making it clear that there is no word on who will be returning for season 21 at this time. Most notably, Ellen Pompeo, the shows original star, took on a reduced role in recent seasons. Find out how many episodes shes appearing in for season 20, airing every Thursday on ABC.

This is the second TV show renewed by ABC today!

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Greys Anatomy Renewed for Season 21 By ABC! | ABC, Greys Anatomy - Just Jared

Grey’s Anatomy future revealed after season 20 – Digital Spy

Grey's Anatomy has been officially renewed by ABC.

The network announced on Tuesday (April 2) that it has picked up a 21st season of the medical drama, with Deadline reporting it's likely to have 18 episodes.

Fans will be thrilled about the renewal, as well as the news that there will be a major increase from only 10 episodes in season 20 due to the WGA and SAG-AFTRA strikes.

Grey's has gone through major changes these past few seasons, with Meg Marinis taking over for long-time showrunner Krista Vernoff and Ellen Pompeo only making guest appearances on screen.

Series creator Shonda Rhimes announced the season 21 renewal: "The loyalty and love of Greys Anatomy fans has propelled us into a historic 21st season, and I could not be more grateful.

Related: Grey's Anatomy's Jessica Capshaw responds to fan reaction ahead of show return

"Meg Marinis storytelling is a gift that continues to keep the show vibrant, compelling and alive, and I cant wait to see what she has in store for next season.

Deadline has reported that producers will begin trying to sign up the veteran cast to new contracts because many of their deals are up after season 20. The list includes original cast members Chandra Wilson (Dr Bailey) and James Pickens Jr (Dr Webber), plus Kevin McKidd (Dr Hunt), Kim Raver (Dr Altman), Camilla Luddington (Dr Wilson) and Caterina Scorsone (Dr Shepherd).

Showrunner Meg Marinis recently suggested that it was likely that Grey's Anatomy would continue beyond its current season.

"I've not been informed that this is the last season, so I'm going to keep going and telling stories as long as they let me. We still have stories to tell," she told Deadline.

Related: Grey's Anatomy's Kevin McKidd talks Teddy and Owen's future after medical emergency

"We receive a tremendous amount of support from the studio and the network and from Shondaland. So I'm going to keep going until they tell me to put that pen down."

While Grey's Anatomy will definitely be continuing, spin-off series Station 19 will be coming to an end later this year after seven seasons.

Grey's Anatomy season 20 and Station 19's final season are airing on ABC in the US. Both shows stream on Disney+ in the UK.

Night News Editor

Justin has been with Digital Spy since 2010, and in that time, has covered countless major news events for DS from the US.

He has worked previously as both a reporter and sub editor for the brand, prior to taking on the position of Night News Editor in 2016.

Over more than a decade, he has interviewed a wide-ranging group of public figures, from comedian Steve Coogan to icons from the Star Trek universe, cast members from the Marvel Cinematic Universe and reality stars from numerous Real Housewives cities and the Below Deck franchise. As a US contributor to Digital Spy, Justin has also been on the ground to cover major pop culture events like the Star Wars Celebration and the D23 Expo.

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Grey's Anatomy future revealed after season 20 - Digital Spy

Grey’s Anatomy’s Jessica Capshaw on fan reaction ahead of show return – Digital Spy

Grey's Anatomy star Jessica Capshaw has responded to the fans' reaction ahead of her anticipated return to the show.

The actress is set to reprise her role as Dr Arizona Robbins on US screens this week (April 4), as part of the long-running medical drama's 20th season.

The beloved paediatric surgeon was last seen in 2018, when she left Seattle for New York to be closer to daughter Sofia amidst her divorce from Dr Callie Torres (Sara Ramrez).

Related: Grey's Anatomy boss teases show future after season 20

Speaking to The Wrap ahead of Arizona's return to Grey Sloan Memorial Hospital, Capshaw shared her excitement at the viewers' reaction and explained how the character's return came about.

"The mission was very clear for Arizona to come back at this time where everyone's a little bit world-weary and missing the magic and joy of healing and bring back some of that joy and levity," she said.

"Hearing the reactions from viewers feeling so excited to reconnect with her made my heart grow a size for sure."

Upon her return, Arizona is set to work on a challenging case with her former colleagues Dr Miranda Bailey (Chandra Wilson), Dr Jo Wilson (Camilla Luddington) and Dr Amelia Shepherd (Caterina Scorsone).

Related: Grey's Anatomy's Kevin McKidd talks Teddy and Owen's future after medical emergency

Capshaw went on to explain that putting the scrubs back on felt like a "seamless" experience, adding: "I put on my costume and I stepped onto the stage and it was just fun. There was no wanting to throw up or die, no scariness.

"It was just like being [back] with friends. The first day was full of reunions, every time I walked around a corner, there'd be a different person that I would just scream with excitement about being able to see."

She concluded: "I have tremendous and deep respect for all the changes that have happened, so I felt very much like this is a house that someone else lives in and I'm coming to visit so let's have the best party while I'm here."

Grey's Anatomy season 20 and Station 19's final season air on ABC in the US. Both shows stream on Disney+ in the UK.

Sara Baalla is a freelance news reporter for Digital Spy.

Excerpt from:
Grey's Anatomy's Jessica Capshaw on fan reaction ahead of show return - Digital Spy

Shay Soker, PhD, inducted into the College of Fellows for the American Institute for Medical and Biological Engineering – EurekAlert

image:

Shay Soker, PhD

Credit: Wake Forest Institute for Regenerative Medicine

Winston-Salem, North Carolina April 2, 2024 Dr. Shay Soker, a distinguished professor at the Wake Forest Institute for Regenerative Medicine (WFIRM), has been honored with induction into the 2024 Class of College of Fellows for the American Institute for Medical and Biological Engineering (AIMBE). This recognition highlights Dr. Soker's contributions to the field of regenerative medicine and his dedication to advancing biomedical engineering.

Dr. Soker's extensive research portfolio spans various critical areas within regenerative medicine, including the identification of novel cell sources, the development of innovative scaffolds for tissue engineering, tissue neovascularization, real-time imaging technologies, and the fabrication of bioengineered tissues for both developmental and disease modeling.

Induction into the AIMBE College of Fellows stands as a top achievement for medical and biological engineers, reserved for the top two percent in these fields, recognizing individuals who have made exceptional contributions to research, practice, or education in engineering and medicine. The AIMBE acknowledges the pioneering work of researchers advancing new technologies and methodologies, whether through traditional fields or through innovative approaches to bioengineering education.

Dr. Sokers pioneering work has significantly advanced our understanding of tissue regeneration and has led to transformative breakthroughs in the field. Notably, Dr. Soker's research has led to the use of vascularized scaffolds for whole organ bioengineering, offering promising prospects for the development of viable organ replacements and regenerative therapies, stated Dr. Anthony Atala, Director of WFIRM.

Commenting on his induction into the AIMBE College of Fellows, Dr. Soker expressed profound gratitude and emphasized the collaborative efforts of his team and colleagues. He stated, "It is a tremendous honor to be recognized by the AIMBE, and I am deeply grateful for the support of my colleagues and collaborators who have contributed to our shared pursuit of advancing regenerative medicine."

Fellows with AIMBE include members from over 30 countries employed in industry, healthcare, academia and government.

Dr. Shay Soker received his PhD from the Technion-Israel Institute for Technology, followed by a postdoctoral fellowship at the Childrens Hospital Boston and Harvard Medical School. He was then recruited to the Laboratory for Tissue Engineering and Cellular Therapies and promoted to Assistant Professor of Surgery at the Harvard Medical School. Currently, Dr. Soker is a Professor of Regenerative Medicine and the Chief Science Program Officer at the Wake Forest Institute for Regenerative Medicine.

About the American Institute for Medical and Biological Engineering (AIMBE): AIMBE is the authoritative voice and advocate for the value of medical and biological engineering to society. AIMBEs mission is to recognize excellence, advance public understanding, and accelerate medical and biological innovation. No other organization brings together academic, industry, government, and scientific societies to form a highly influential community advancing medical and biological engineering. AIMBEs mission drives advocacy initiatives into action on Capitol Hill and beyond. For more information, visit http://www.aimbe.org.

Media contact: Charlie Kim, ckim@aimbe.org

About Wake Forest Institute for Regenerative Medicine: The Wake Forest Institute for Regenerative Medicine is recognized as an international leader in translating scientific discovery into clinical therapies, with many world firsts, including the development and implantation of the first engineered organ in a patient. Over 500 people at the institute, the largest in the world, work on more than 40 different tissues and organs. A number of the basic principles of tissue engineering and regenerative medicine were first developed at the institute. WFIRM researchers have successfully engineered replacement tissues and organs in all four categories flat structures, tubular tissues, hollow organs and solid organs and 16 different applications of cell/tissue therapy technologies, such as skin, urethras, cartilage, bladders, muscle, kidney, and vaginal organs, have been successfully used in human patients. The institute, which is part of Wake Forest University School of Medicine, is located in the Innovation Quarter in downtown Winston-Salem, NC, and is driven by the urgent needs of patients. The institute is making a global difference in regenerative medicine through collaborations with over 500 entities and institutions worldwide, through its government, academic and industry partnerships, its start-up entities, and through major initiatives in breakthrough technologies, such as tissue engineering, cell therapies, diagnostics, drug discovery, biomanufacturing, nanotechnology, gene editing and 3D printing.

Media contact: Emily Gregg, egregg@wakehealth.edu

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.

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Shay Soker, PhD, inducted into the College of Fellows for the American Institute for Medical and Biological Engineering - EurekAlert

Student receives MaGNET Award to present research at international genetics conference – News at OU

Bioengineering student Daniela Meson De La Fuente is pictured with the research poster she presented at the 2024 Maize Genetics Meeting.

Oakland University student Daniela Meson De La Fuente recently presented research at the 2024 Maize Genetics Meeting, an international conference that brought together researchers whose work advances the field of maize (corn) genetics and breeding.

The sophomore bioengineering major attended the meeting in Raleigh, North Carolina after receiving a MaGNET Award, a competitive travel grant aimed at members of underrepresented groups.

I was extremely excited when I received the news, as only five undergraduates received the awardfrom hundreds of attendees, she said. This award was funded by the National Science Foundation to create diversity in our future researchers.

As a member of Dr. Shailesh Lals research laboratory, Meson De La Fuente is researching a gene that exists in corn and humans, serving important functions for both.

Our laboratorys prior work led to the discovery of a novel RBM48 gene in maize, which was later found to be homologous in humans, she said. A mutated RBM48 gene can lead to developmental defects in both maize and humans. It is likely that this gene is associated with developing diseases, including cancer.

While working in the lab, Meson De La Fuente has been mentored by Dr. Lal and graduate student Dalton Raymond.

Dalton has been my mentor since the summer of 2023, when I was accepted to be part of the Summer Undergraduate Research Program (SURP), she said. He had been working on this research project since I joined the lab team, and I helped him continue the project.

She also credited Dr. Lal for encouraging her to apply to SURP, as well as for the MaGNET Award.

Heexemplifies a professor who goes above and beyond to ensure his students have everything they need toachieve their goals, she shared. I like that Oakland University has countless opportunities for undergraduate students to explore theirinterests by getting involved in research, something that is very rare and unique to be available for an early-career student.

To receive a MaGNET Award, Meson De La Fuente submitted a detailed application, including information about her research, academic performance, career aspirations and a recommendation letter from Dr. Lal.

"Daniela is a dedicated studentwho excels inacademic and extracurricular activities, said Lal, professor and chair of OUs Department of Bioengineering.

He lauded her strong leadership qualities, including her roles as an OU Student Congress legislator,treasurer of the OU Engineering in Medicine and Biology Society, member of the OU Chapter of Sigma Xi Scientific Society, andmember of the Society for Advancement of Chicanos/Hispanics and Native Americans in Science.

She has a bright career ahead of her in whatever she decides to pursue, Lal added.

At the conference, Meson De La Fuente presented her work on developing a method to discover the cause of the RBM48 gene mutations associated with human disease. The data generated from the study could help pave the way for development of treatments for certain cancers.

Along with sharing her research, she also learned from other student researchers and gained insight into how companies are leveraging the latest advances in agricultural science.

I hadan amazing experience at the meeting. I not only got an inside view of how other student presenters came about pursuing a master's or Ph.D., which was very helpful, but I also had the opportunity to connect and network with businesses such as Syngenta and Corteva, Inc. and learn about their innovative agricultural projects, she said. I met people from universitiesworldwide,which broadened my perspective on collaboration opportunities in research.

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Student receives MaGNET Award to present research at international genetics conference - News at OU

RIT faculty member becomes fellow of the American Institute for Medical and Biological Engineering | RIT – Rochester Institute of Technology

Karin Wuertz-Kozak, a faculty researcher at Rochester Institute of Technology, was recently inducted into the American Institute for Medical and Biological Engineering (AIMBE) College of Fellows. Becoming an AIMBE Fellow, one of the organization's most prestigious honors, signifies inclusion among the top 2 percent of medical and biological engineers, representing the most accomplished individuals across academia, industry, education, clinical practice, and government.

Wuertz-Kozak, a Kate Gleason Endowed Professor in RITs Department of Biomedical Engineering, was recognized for her outstanding contribution to understanding the pathophysiology of degenerative disc disease and to developing new therapeutic strategies for disc disease. She brings an interdisciplinary background to her work with experience in pharmacology, biomedical engineering, and biology, as well as business administration. Her clinical collaborations are worldwide, spanning locally with the University of Rochester to the Fukushima Medical University in Japan.

Karin is conducting important research that will ultimately improve the quality of life for individuals suffering from degenerative disc disease, said Doreen Edwards, dean of RITs Kate Gleason College of Engineering.We are thrilled that she is being honored with this prestigious award.

Wuertz-Kozak is leading research on the role and effects of mechanical loading in the context of back pain. Understanding the mechanisms leading to degeneration and chronic inflammation can give clues to relieving disc-related back pain and is a crucial part of developing novel, molecular treatment options for patients, she said.

One promising approach being developed by her research team is to modulate and control tissue inflammation and induce regeneration is through extracellular vesicles (EVs) derived from CRISPR-modified stem cells. While stem cells have proven successful in the regeneration of many tissues, the intervertebral disc constitutes a drastically harsh cell environment, making EV therapy a promising alternative to cell therapy. To this end, she has received several significant research grants from prominent agencies such as the National Science Foundation and the National Institutes of Health.

A longtime member of several national and international associations, Wuertz-Kozak has held leadership positions with the International Society of the Study of the Lumbar Spine and the Orthopedic Research Society. Among her many academic achievements, Wuertz-Kozak has been recognized by the Swiss National Science Foundation Professorship Award in 2016 and received a Faculty Scholarship Award as part of RITs Kate Gleason College of Engineering in 2021. She has published more than 90 peer-reviewed articles and has contributed to numerous journals related to molecular science and biomedical engineering in editorial roles and as a reviewer.

AIMBE Fellows are employed in academia, industry, clinical practice, and government., and consist of distinguished medical and biological engineers including three Nobel Prize laureates and 22 Presidential Medal of Science and/or Technology and Innovation awardees. Each has led initiatives to pioneer new and developing fields of technology, making major advancements in traditional fields of medical and biological engineering, or developing/implementing innovative approaches to bioengineering education.

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RIT faculty member becomes fellow of the American Institute for Medical and Biological Engineering | RIT - Rochester Institute of Technology

Bioengineering of vascularized porcine flaps using perfusion-recellularization | Scientific Reports – Nature.com

Animal use

Yorkshire pigs (3040kg; age approximately 12weeks old) were used for all decellularization and recellularization experiments. All studies were approved by the Institutional Animal Care and Use Committee (IACUC) of the University Health Network and Toronto General Hospital Research Institute. Humane care was provided to all animals in accordance to the Principles of Laboratory Animal Care defined by the National Society for Medical Research and the Guide for the Care of Laboratory Animals issued by the National Institutes of Health. Reporting of use of experimental animals in this study followed recommendations specified by the ARRIVE guidelines.

Pigs were fasted for 12h prior to surgery. Sedation was achieved with ketamine (20mg/kg IM), atropine (0.04mg/kg IM) and midazolam (0.3mg/kg IM). Anesthesia was induced by inhalation of 5% isoflurane through a mask at a flow rate of 22 to 44mL/kg/min to facilitate peripheral line insertion and intubation. Anesthesia was maintained with isoflurane (0.5 to 2%). Pigs were intubated with an appropriate endotracheal tube (78mm) and ventilated to a tidal volume of 8mL/kg, positive end-expiratory pressure of 5cm H2O, FiO2 of 0.5 and respiratory rate of 14 breaths per minute. Pigs were prepped and draped in the usual sterile fashion prior to flap procurement. Surgical procedure for porcine omentum and TFL flaps procurement were as previously described30. Briefly, the omental flap was procured by midline laparotomy and the left gastroepiploic artery and vein was used as the dominant vascular conduit. The right gastroepiploic vessels were ligated to prevent perfusion flow-through.

The TFL flap was procured with pigs in the lateral decubitus position. The main vascular pedicle was defined by the ascending branch of the lateral circumflex femoral artery and veins. The overlying skin island was removed to produce a pure fascial flap. Following flap detachment, the vascular pedicle was cannulated with 2022 G Angiocath (Becton Dickenson) under direct vision and flushed with 20 U/mL heparin sodium (LEO Pharma, Denmark) in 0.9% normal saline and transported under sterile conditions to the lab.

Porcine flaps were perfusion-decellularized using low-concentration SDS followed by DNase (Sigma Aldrich) reconstituted to a concentration of 10mg/mL, as previously described30. Cannulated flaps were each connected to a perfusion system to allow antegrade perfusion via the arterial inlet at 2ml/min, in which solutions: 0.05% SDS followed by 0.1mg/mL deoxyribonuclease (DNase) were perfused through the flap vasculature with 1phosphate buffered saline (PBS) perfusion in between to remove residual detergent. Flaps were sterilized in 0.1% paracetic acid (PAA) / 4% ethanol (EtOH) (Sigma Aldrich) and then washed in 1PBS prior to recellularization. As described previously30, omental and TFL flaps were perfused with SDS for 2 and 3days, respectively. Following SDS perfusion, flaps were washed with PBS for 24h and then perfused with DNase for 2h, PBS for again for 24h, and finally PAA/EtOH for 3h. With the exception of DNase, each step included an exchange of the submersion fluid to match the given perfusate. For the DNase step, flaps were submerged in fresh PBS.

Commercially available HUVECs (American Type Culture Collection/ATCC, USA) were cultured in EGM-2 (Lonza, Switzerland) supplemented with SingleQuots (Lonza) of Growth Supplements including: FBS 2%, hEGF, hydrocortisone, Gentamicin/Amphotericin-B, VEGF, hFGF-B, R3-IGF-1, ascorbic acid, and heparin (concentrations proprietary). Commercially obtained human bone-marrow derived MSCs (Promocell, Germany) were cultured in MSCGM (Promocell) containing proprietary media supplement and 5% FBS. HMSCs and HUVECs between passage 4 and 6 were used for recellularization. Both cell types were verified for correct functional and phenotype expression. HUVECs expressed CD31/VE-Cadherin using flow cytometry and were functionally capable to undergo angiogenesis. MSCs were CD90/73/44 positive and CD34/45/11b negative using flow cytometry and capable of undergoing trilineage differentiation (Supplementary Fig.1). These findings were consistent with the minimal criteria to define MSCs according to the International Society for Cellular Therapy Criteria47.

All cells were maintained in 150 cm2 dishes until reaching 90% confluency (resulting in approximately 50,000 cells/cm2). Cells were detached from culture vessels with 0.25% trypsinEDTA solution (Gibco) prior to recellularization. Cell media was replaced every other day, and the cultures were maintained in a humidified 95% air/5% CO2 incubator at 37C.

A closed-system bioreactor was set up in an incubator for recellularization within the flap scaffold matrix. We used a modified airtight snap-lid container, previously used for decellularization with a closed-circuit L/S-16 (Masterflex, Fisher Scientific) silicone tubing. The end of the tubing external to the tissue chamber was fitted with a female Luer thread-style panel (Cole-Parmer), which connected to a 3-stop tubing compatible with peristaltic pump (Ismatec, Cole-Parmer) tubing cassette as previously used for perfusion-decellularization. The opposite end of tubing was reconnected to the second port from the tissue chamber to allow closed-loop circulation of medium from tissue chamber into the flap via the arterial cannula at a flow rate of 2mL/min. Just proximal to the tissue chamber, silicone tubing was connected to a three-way stopcock (Baxter, USA). The chamber was filled with 200mL of EGM-2 media, which was primed through the tubing to remove air bubbles. Decellularized flaps were perfused with EGM-2 at 2mL/min in conventional cell culture incubator at standard conditions (95% air/5% CO2) overnight before cell seeding to equilibrate flaps with culture medium.

Cell seeding was performed as follows: HUVECs and human bone-marrow derived MSCs were lifted from tissue culture plastic with 0.25% trypsin and centrifuged at 500g for 5min. The resultant cell pellet was resuspended in 10mL media, strained with 75m pore mesh, and counted via automated hemocytometer (Vi-Cell XR, Beckman Coulter). A total of 8107 cells, divided equally with 4107 HUVEC co-cultured with 4107 MSCs, were used for recellularization of each scaffold. A combined cell suspension of the two cells were slowly manually injected into the vascular arterial inlet through a three-way stopcock. Following the introduction of cells, flaps were placed in a standard cell culture incubator for 2h of static culture to allow cell attachment. Afterwards, perfusion-culture was initiated with the peristaltic pump (Ismatec, Cole-Parmer) running at 2mL/min for 6days. Media passed through the flap was recovered back into the reservoir using a separate pump channel that drained the bioreactor at an equal rate to the perfusion, allowing for recycling and reuse. Media was exchanged every other day for fresh EGM-2. A total of 750mL of culture medium was used over 6days for each flap.

Native, decellularized, and recellularized tissues were biopsied near the distal margin of the flap, fixed in 10% formalin (Fisher Scientific), embedded in paraffin, and sliced into 5m sections on microtome (Leica Biosystems). Slides of the paraffin-embedded samples were processed for histological and IHC staining. Histologic staining was performed on xylene-deparaffinized slides with the following stains: H&E (Sigma Aldrich), Massons Trichrome (American MasterTech Scientific), and Verhoeff Van Gieson Elastin Stain (Abcam).

For IHC, heat induced antigen retrieval was done with citrate buffer (pH 6.0; Thermo Fisher Scientific) in a 95C autoclave for 10min. Endogenous peroxidases were blocked with a peroxide block (Cardinal Health), and nonspecific binding was blocked with Dako Serum-Free Protein-Block (Agilent). Sections were incubated with the primary antibodies at 4C overnight with dilutions as follows: rabbit polyclonal anti-Collagen IV (Abcam, ab6586, 1:300), rabbit polyclonal anti-Fibronectin (Abcam, ab23751; 1:400); and rabbit polyclonal anti-Laminin (Abcam, ab11575, 1:400) and anti-CD31 (Abcam, ab28364, 1:50) at 4C overnight. Slides were washed three times in PBS with 0.1% Tween and goat anti rabbit IgG HRP-conjugated secondary antibody (ImmPRESS Peroxidase Polymer Reagent, Vector Laboratories) was applied for 30min. Slides were again washed thrice in PBS-Tween and then diaminobenzidine solution (Vector Laboratories) applied for 10min. Slides were counterstained with hematoxylin. After staining, all slides were dehydrated in ethanol to xylene exchange, mounted and imaged on Aperio CS2 Slide Scanner (Leica Biosystems).

Immunofluorescence staining was performed using paraffin embedded sections cut to 5m thickness and deparaffinized using xylene and rehydrated in serial dilutions of ethanol. Tissue sections in were incubated in antigen retrieval buffer (10mM citrate buffer, pH 6.0) at 95C for 10min in an autoclave. Tissue sections were then blocked with 5% blocking serum (goat serum) in 1% bovine serum albumin (BSA) before adding primary antibody. Slides were then incubated with primary antibodies for VE-Cadherin (Abcam, ab33168, 1:100) and vimentin (Abcam, ab92547, dilution 1:200) diluted in 1% BSA at 4C overnight. After washing three times with PBS-Tween, slides were then incubated for 1h at RT in the secondary antibody goat anti-rabbit IgG conjugated with AlexaFluor 647 (Thermo Fisher Scientific, 1:500). Finally, slides were washed three times with PBS-Tween in the dark and counterstained with DAPI (Abcam; 1:5000). Negative controls were used by replacing the primary antibody with the corresponding isotype (IgG) of the primary antibody. Images were taken on a Leica SP8 confocal microscope with LAS X software (Leica Biosystems) installed.

Tissue pieces (~3040mg) were obtained by punch biopsy tool and dried in 60C oven overnight. Dried tissue pieces were digested in papain solution at 65C for 18h. Corresponding native flap tissues were dried and digested in parallel as controls. Papain (Sigma Aldrich, 16 units/mg protein) 1530mg/mL stock was solubilized to working concentration of 0.1mg/ml in 0.1M phosphate buffer (pH 6.0), with 5mM cysteine hydrochloride (Sigma Aldrich), and 5mM EDTA (Sigma Aldrich). The lysates were used for detection of sulfated glycosaminoglycan (sGAG) and DNA content. The Blyscan Sulfated GAG Assay kit (Biocolor) was used to measure sGAG according to manufacturers instruction. Briefly, tissue specimen lysates were mixed with Blyscan Dye Reagent to bind the GAG for 1h at room temperature. The GAG-dye complex was then collected by centrifugation at 10,000g. After the supernatant was removed and the tube drained, Dissociation Reagent was added and 100l of analyte solution was transferred to a 96-well plate. Absorbance against the background control was obtained at a wavelength of 656nm with a SpectraMax spectrophotometer (Molecular Devices). GAG amount was interpolated from a standard curve (05g) using a known GAG standard provided in the kit. Final GAG content was standardized to the total dry tissue mass (mg) used for assay.

For DNA content quantitation, the tissue lysate following papain digestion (above) was used. The Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) was used to measure DNA content according to manufacturers instruction. Fluorescence reading (excitation: 485nm and emission: 528nm) was taken on a plate reader (Cytation 5, Biotek), and the absolute amount of DNA (ng) was quantified against a lambda DNA standard curve (01000ng) provided by the manufacturer; final DNA content was standardized to total dry tissue mass (mg) used for assay.

All statistical analysis was performed using GraphPad Prism, version 9.0 (GraphPad, Inc.). Statistical analyses was conducted with multiple unpaired t test with a significance level of p<0.05. Values are presented as mean, with S.D. unless stated otherwise.

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Bioengineering of vascularized porcine flaps using perfusion-recellularization | Scientific Reports - Nature.com

Sherry Gao pushes the boundaries of genetic engineering | Penn Today – Penn Today

Sherry (Xue) Gao, Presidential Penn Compact Associate Professor in Chemical and Biomolecular Engineering (CBE)in the School of Engineering and Applied Science, always knew she had a future in the lab. I grew up in China, and when I was little, maybe 6 or 7, she recalls, my teacher asked me, What do you want to be when you grow up? I said, I want to be a scientist.

Neither of her parents had studied beyond high school; when Gao finished her training as a chemical engineer, she became the first person in her family to graduate from college. One of my greatest motivations is to help first-generation college students, Gao says.

Now, as the newest faculty member in CBE, Gao is prepared to do just that: support the next generation of chemical engineers, while also conducting groundbreaking research in the development of small molecules to edit genes, pushing the boundaries of precision medicine.

One of Gaos primary goals is to make gene-editing tools more accurate. As Gao points out, CRISPR, the revolutionary technology developed by Nobel Prize winners Jennifer Doudna and Emmanuelle Charpentier, doesnt always work perfectly. The tool goes in, fixes a mutation, but we also observe a lot of off-targets, Gao says. So its not just hitting the target letters in our genetic code, its sometimes editing other places. You could cure one genetic disease by using the CRISPR tools, but then the off-targets could cause dozens of other problems.

More generally, Gao is fascinated by enzymes, the class of molecules to which CRISPR belongs, which enable chemical reactions by lowering the activation energy required for a reaction to take place. Enzymes typically catalyze molecules in a very precise fashion, says Gao. Thats sort of my passion: to look into how nature makes some molecules so accurate, and how we as humans and engineers can learn from that.

This story is by Ian Scheffler. Read more at Penn Engineering Today.

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Sherry Gao pushes the boundaries of genetic engineering | Penn Today - Penn Today

Research of extraordinary scope and rigorDan Schwartz honored by American Chemical Society – University of Colorado Boulder

Photo caption: The Schwartz lab discovered that molecules move around on surfaces via a complex type of motion involving crawling, hoppingand flying.

Professor Dan Schwartz

Professor Daniel K. Schwartz has been honored with the prestigious American Chemical Society (ACS) Division of Colloid and Surface Chemistry 2024 Langmuir Lectureship award. He was nominated by his colleagues for significant contributions to the field of colloid and interface science.

Colloids are mixtures in which one substance is finely dispersed in another substance. Interface science refers to the boundaries between different phases of matter, such as between two unmixable liquids, or between a liquid and a solid.

Schwartz, a professor in CU Boulders Department of Chemical and Biological Engineering, said the award was significant for several reasons.

Most importantly, it recognizes the excellence of research performed by my PhD students and postdocs, past and present, he said. The recognition is also special because it is sponsored jointly by the ACS Division of Colloid and Surface Chemistry and the ACS journal Langmuir, both of which are very close to my heart. The namesake of the award, Irving Langmuir, a Nobel laureate and the foundational figure of surface science, is a long-time scientific hero of mine.

Schwartz will receive a commemorative plaque, complimentary registration and reimbursement for travel expenses to the ACS fall 2024 meeting and a $3,000 award. He will also deliver a special lecture at the ACS fall 2024 symposium.

Schwartzs colloid and interface science research carries significant practical implications for various fields. These include membrane-separation processes and biocatalysis applications such as water purification, wastewater treatment, food and beverage processing and pharmaceutical manufacturing. His work also extends to chemical production as well as environmental remediation and biofuel synthesis.

Dans contributions to fundamental understanding of dynamic interfacial phenomena are extraordinary, the nominators said in a letter to the selection committee. He has provided new windows into monolayers at interfaces, on solid boundaries and new approaches to understanding fundamental transport of confined molecules, nanoparticles and active particles in porous media. This work is of extraordinary scope and rigor.

The award also entails an expectation that Schwartz will submit a feature article for publication in Langmuir within six months following his lectureship presentation.

It is incredibly satisfying to share the award with my PhDs and postdocs, Schwartz said. Im eagerly looking forward to the opportunity to describe their work to the award lecture audience in August.

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Research of extraordinary scope and rigorDan Schwartz honored by American Chemical Society - University of Colorado Boulder

New tools reveal how genes work and cells organize – EurekAlert

image:

Circular dichroism showing folding of RNA into G-quadruplexes at different temperatures

Credit: Luige et al. 2024

Proteins binding to RNA are important in many processes in the cell and can mediate a range of biological functions. A specialized structure in both DNA and RNA, the G-quadruplex, are regulatory elements involved in gene expression in both DNA and RNA. In the present work the researchers use theoretical predictions and molecular biology experiments to show that many chromatin-binding proteins bind to RNA G-quadruplexes. With this information they can classify proteins based on their potential to bind RNA G-quadruplexes.

The study uses a combination of experimental identification of RNA G-quadruplex-binding proteins and computational methods to build a prediction tool that identify the probability that a protein binds to RNA G-quadruplexes. The findings show that predicted proteins show a high degree of protein disorder and hydrophilicity, suggesting an involvement in both transcription and phase-separation into membrane-less organelles.

Ulf roms group has previously shown that RNA-DNA dual binding proteins are likely to have an involvement in the DNA damage response, linking DNA and RNA binding properties to a number of proteins. In the new study, the researchers expanded the knowledge of RNA-binding proteins to identify RNA G-quadruplex binding proteins.

The researchers have also developed a computational tool to assess RNA G-quadruplex-binding potential of proteins that can be accessed athttp://service.tartaglialab.com/new_submission/clever_G4_classifier.

With these new results, the researchers identify properties of protein-RNA interactions, and provide means to identify G-quadruplex binding properties that can potentially be targeted therapeutically in disease.

The findings have just been published inNature Communications.

Nature Communications

Cells

22-Mar-2024

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.

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New tools reveal how genes work and cells organize - EurekAlert

Learn About Undergraduate Research Opportunities in Chemistry April 9 – University of Arkansas Newswire

Chemistry Club

The Chemistry Club would like to invite current members and any undergraduate student interested in chemistry/biochemistry research or looking to join a lab for a research exposition Tuesday, April 9, from 5-6:30 p.m. at CORD 127.

The Chemistry Club would like to invite current members and any undergraduate student interested in chemistry/biochemistry research or looking to join a lab for a research expositionfrom 5-6:30 p.m. Tuesday, April 9, at the Cordia Harrington Center, room 127. Food will be provided!

Students from any major can come and hear firsthand from other undergraduates and graduate students about the exciting research happening in the Chemistry Department, as well as their experiences in lab and advice on joining a lab. There will be presentations and posters showcasing the variety of opportunities for undergraduates to get involved in research. Students already in a lab are welcome to join to hear more about department research, and those interested in eventually completing an honors thesis in chemistry or biochemistry are highly encouraged to attend. You do not have to be a chemistry/biochemistry major to attend, as several labs in the department welcomingly take students of other majors!

This event is supported by the Student Activities Fee as a funded event by the Associated Student Government and is free to all currently enrolled University of Arkansas, Fayetteville, students who pay the student activities fee. This event is held in a venue that meets ADA standards. Individuals with disabilities are encouraged to attend this event. If you require a reasonable accommodation in order to participate in this event, please contact Ethan Batey at jebatey@uark.edu or (307)-277-2812 by five business days prior to the event.

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Learn About Undergraduate Research Opportunities in Chemistry April 9 - University of Arkansas Newswire

Effect of gamma rays on the essential oil and biochemical characteristics of the Satureja mutica Fisch & C. A. Mey … – Nature.com

Mozaffarian, V. A Dictionary of Iranian Plant Names: Latin, English, Persian. 1740 (Farhang Mo'aser Press, 1996) (in Persian).

Saeidinia, M., Hosseinian, S. H., Beiranvand, F. & Mumivand, H. Study of the essential oil, morphological parameters, and growthstage specific crop coefficients of summer Savory (Satureja hortensis L.). J. Med. Plants By-Prod. 1, 16 (2019).

Google Scholar

Caprioli, G., Lupidi, G. & Maggi, F. Comparison of chemical composition and antioxidant activities of two Winter savory subspecies (Satureja montana subsp. anacetum and Satureja montana subsp. Montana) cultivated in Northern Italy. Nat. Prod. Res. 33, 15 (2018).

Google Scholar

Shariat, A., Karimzadeh, G., Assareh, M. H. & Hadian, J. Metabolite profiling and molecular responses in a drought-tolerant savory, Satureja rechingeri exposed to water deficit. Biotechnology 8(11), 477 (2018).

Google Scholar

Bozorgmehr, A., Abasi, M. & Ebrahimi, T. Protection of medicinal ash (Fraxinus excelsior). In National Conference on Medicinal Plants, Islamic Azad University, Science and Research Ayatollah Amelie. http://www.civilica.com/Paper-ERBAL03_031.html (2013).

Pezantes-Orellana, C., German Bermdez, F., Matas De la Cruz, C., Montalvo, J. L. & Orellana-Manzano, A. Essential oils: A systematic review on revolutionizing health, nutrition, and omics for optimal well-being. Front. Med. 11, 1337785 (2024).

Article Google Scholar

Bunse, M. et al. Essential oils as multicomponent mixtures and their potential for human health and well-being. Front Pharmacol. 13, 956541 (2022).

Article CAS PubMed PubMed Central Google Scholar

Tomas, S.C.L. Medicinal Plant. 1225. (Technomic Publication, 2000)

Gadjalova, A. V. & Mihaylova, D. Ultrasound-assisted extraction of medicinal plants and evaluation of their biological activity. Food Res. 3, 530536 (2019).

Article Google Scholar

Pandey, A. K. & Das, R. Good field collection practices and quality evaluation of medicinal plants: Prospective approach to augment utilization and economic benefits. Res. J. Med. Plants 8, 119 (2014).

Article Google Scholar

Vasanthkumar, S.S. Post-harvest processing of medicinal plants. In Horticulture Science (Royal Book Publishing, 2023).

Pajohesh, A. Applications of nuclear energy in agriculture. In Special Week of Agriculture. Public Relations Research and Education Agriculture (2007).

Ibrahim, A. M. M. et al. Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae). J. Appl. Res. Med. Aromat. Plants 8, 2632 (2018).

Google Scholar

Verma, A. K., Sharma, S., Kakani, R. K., Meena, R. D. & Choudhary, S. Gamma radiation effects seed germination, plant growth and yield attributing characters of fennel (Foeniculum vulgare Mill.). Int. J. Curr. Microbiol. Appl. Sci 6, 24482458 (2017).

Article CAS Google Scholar

Jafari Ghoshchi, M., Abbaszadeh, B., Oraei, M., Azimi, R. & Faramarzi, A. Study on gamma ray drying method and conditions and storage duration effects on essential oil and some physiological traits of Satureja spicigera (C. Koch) Boiss. Iran. J. Med. Aromat. Plants. Res 38, 200217 (2022).

Google Scholar

Hossain, M. B., Barry-Ryan, C., Martin-Diana, A. B. & Brunton, N. P. Effect of drying method on the antioxidant capacity of six Lamiaceae herbs. Food Chem. 123, 8591 (2010).

Article CAS Google Scholar

Salehi, F., Khairi, A., Eskandari, A. & Rezaei, M. B. The effect of gamma ray irradiation on the morphological and phytochemical traits of the medicinal plant Thymus vulgaris L.. Ecophytochem. Med. Plants 3, 1021 (2014).

Google Scholar

IRMO. Islamic Republic of Iran Meterological Organization Climatology Methods. https://www.IRMO.ir (2015).

HosseinzadehRostamKalaei, M., Abdossi, V. & Danaee, E. Evaluation of foliar application of selenium and flowering stages on selected properties of Iranian Borage as a medicinal plant. Sci. Rep. 12, 12568 (2022).

Article ADS CAS Google Scholar

Adams, R.P. Identification of Essential Oil Components by Gas ChromatographyMass Spectroscopy. 1804 (Allured Publishing Corporation, 2017).

Singleton Vernon, L., Rudolf, Q., Lamuela-Ravents, S. & Rosa, M. Analysis of total phenolic contents and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Int. J. Chem. Biol. 99, 99017 (1999).

Google Scholar

Rezaei Nazari, M., Abdossi, V., Zamani Hargalani, F. & Larijani, K. Antioxidant potential and essential oil properties of Hypericum perforatum L. assessed by application of selenite and nano-selenium. Sci. Rep. 12, 6156 (2022).

Article ADS Google Scholar

Ebadi, M. T. Use of radiation in the industrial processing of medicinal plantsA review. Baghdar Mag. 98, 1320 (2015) ((In Persian)).

Google Scholar

Sayed, T.E. & Ahmed, E.-S.S. Improving artemisinin and essential oil production from Artemisia plant through in vivo elicitation with gamma irradiation nano-selenium and chitosan coupled with bio-organic fertilizers. Front. Energy Res. 10, 996253 (2022).

Aly, A., Mohammed, M., Maraei, R., Abdalla, A. & Abouel-Yazeed, A. Improving the nutritional quality and bio-ingredients of stored white mushrooms using gamma irradiation and essential oils fumigation. Radiochim. Acta 111, 387399 (2023).

Article CAS Google Scholar

Aly, A., Maraei, R., Rezk, A. & Diab, A. Phytochemical constitutes and biological activities of essential oil extracted from irradiated caraway seeds (Carum carvi L.). Int. J. Radiat. Biol. 99, 318328 (2023).

Article CAS PubMed Google Scholar

Subhan, F. et al. Effect of gamma radiation on growth and yield of barley under different nitrogen levels. Pak. J. Biol. Sci. 7, 981983 (2004).

Article Google Scholar

Haddad, M., Herent, M., Tilquin, B. & Quetin Leclercq, J. Effect of gamma and e-beam radiation on the essential oils of Thymus vulgaris thymoliferum, Eucalyptus radiata, and Lavandula angustifolia. J. Agric. Food Chem. 55, 60826086 (2007).

Article CAS PubMed Google Scholar

Belcadi, H. et al. Gamma-irradiation effect on the chemical composition and antibacterial activity of the Tanacetu annuum L. essential oil. Heliyon 9, e16625 (2023).

Article CAS PubMed PubMed Central Google Scholar

Al-Kuraieef, A. & Alshawi, A. The effect of gamma irradiation on the essential oils and antioxidants in dried thyme. Int. J. Food Stud. https://doi.org/10.7455/ijfs/9.1.2020.a6 (2020).

Article Google Scholar

Abdelmoety, A.A., Foda1, F.F.A., El-Hadary, A.E. & Abo-El-Seoud, M.A. Impact of -irradiation on chemical constituents, antibacterial and antioxidant activity of clove and cinnamon volatile oils. J. Agric. Chem. Biotechnol (Mansoura Univ.) 14, 3541 (2023)

Sefidkon, F. & Jamzad, Z. Essential oil composition of Satureja spicigera (C. Koch) Boiss. from Iran. Flavor. Frag. J. 19, 571573 (2004).

Article CAS Google Scholar

Gokturk, T. Chemical composition of Satureja spicigera essential oil and its insecticidal effectiveness on Halyomorpha halys nymphs and adults. Z. Naturforsch. C J. Biosci. 25, 457 (2021).

Google Scholar

Pereira, E. et al. Effects of gamma irradiation on cytotoxicity and phenolic compounds of Thymus vulgaris L. and mentha piperita L.. LWT -Food Sci. Technol 71, 370 (2016).

Article CAS Google Scholar

Abbaszadeh, B., Layeghhaghighi, M., Azim, R. & Hadi, N. Improving water use efficiency through drought stress and using salicylic acid for proper production of Rosmarinus officinalis L.. Ind. Crops. Prod. 144, 111893 (2020).

Article Google Scholar

Kosalec, I., Cvek, J. & Tomi, S. Contaminants of medicinal herbs and herbal products. Arch. Ind. Hyg. Toxicol. 60, 485501 (2009).

CAS Google Scholar

Hidar, N. et al. Comparative study of two preservation methods (freeze drying and gamma irradiation) on the phenolic profile, antioxidant and antimicrobial activities of the essential oils of stevia. J. Food Meas. Character. 17, 36653672 (2023).

Article Google Scholar

Douar-Latreche, S. et al. Effect of gamma irradiation on the chemical composition and antioxidant activity of Thymus algeriensis extracts. J. Essent. Oil-Bear. Plants 21, 449461 (2018).

Article CAS Google Scholar

Sadecka, J., Koleck, E., Petka, J. & Suhai, M. Influence of two sterilization ways on the volatiles of black pepper (Piper nigrum L.). Chem. Listy 99, 335338 (2005).

Google Scholar

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