Webinar from CRi on Quantitating Multiple Protein Expression in Intact Tissue

Event-june_2010_webinar_banner_jpg-632010 Multispectral imaging bridges the gap between ‘omics’ multiplexed array-based techniques and conventional tissue imaging. This webinar provides an overview of how leading scientists are using multi-label imaging in combination with pattern-recognition image analysis software to analyze biomarkers specific to important disease-related cellular states, such as signaling configuration, stem cell pluripotency, and receptor expression.  Call +1-781-935-9099 ext 158 or e-mail us NOW at toleary@cri-inc.com to sign up for this FREE webinar.

Featured Speaker: James Mansfield
Director, Multispectral Imaging Systems
Cambridge Research & Instrumentation, Inc.
Title: Quantitating multiple protein expressions in intact tissue.
Date: Thursday, June 17, 2010
Time: 1:00 PM - 2:00 PM EDT
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CRi technology helps to bridge the gap between advances made in the ‘omics’ multiplexed array-based techniques and conventional tissue imaging. This webinar provides an overview of how leading scientists are using multi-label imaging in intact tissue, in combination with pattern-recognition image analysis software to analyze biomarkers specific to important disease-related cellular states, such as signaling configuration, stem cell pluripotency, and receptor expression.  

Improved specificity in determining disease subclasses through protein expression profiles is a goal of many studies. Obtaining this specific information requires the analysis of multiple proteins on a per-cell basis in intact tissues, from specific architectural contexts.

In pharmaceutical drug development, revealing correlations between protein activity and clinical outcome supports target validation, trial design, patient selection, response assessment, and, if trials are successful, the diagnostic component of theranostics. However, the ability of tools to support these activities depends strongly on their precision and accuracy for quantitating protein labels. Multispectral imaging coupled with automated learn-by-example analysis software offer a powerful combination to generate reproducible data of per-cell phenotypes in tumor regions or other regions of interest. Many techniques deployed today, such as those based on microarray detection, or analysis of sample lysates, provide data that are, in fact, averages from volumes of tissue, including many cells not of interest. These methods blur-out key proteomic information that resides at the cellular level and that relates to the signaling states of individual cells.

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Required: Mac OS® X 10.4.11 (Tiger®) or newer

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Study praises Howard, other minority medical schools – Washington Post


Washington University in St. Louis News
Study praises Howard, other minority medical schools
Washington Post
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The Living Matrix: A Movie Promoting Energy Medicine Beliefs

It’s boring to try to ferret out reliable health information from dry medical journals. It’s easier and more fun to watch a movie. A new movie promises to change the way you think about your health. To bring you breakthroughs that will transform your understanding of how to get well and stay well. To share the discoveries of leading researchers and health practitioners about miracle cures that traditional medicine can’t explain.

If this makes your baloney detector light up, good for you!

The Living Matrix: A Film on the New Science of Healing is an atrociously bad movie that falls squarely in the tradition of What the Bleep Do We Know? In his book Nonsense on Stilts, Massimo Pigliucci characterized the “Bleep” movie as “one of the most spectacular examples of a horribly tangled mess of science and nonsense,” and this new movie is more of the same. Bleep was just silly, but The Living Matrix is potentially dangerous because it might persuade patients to make poor decisions about their medical care.

It purports to be a documentary about the “new science of healing” but really amounts to an infomercial for various forms of quackery based on so-called “energy medicine.” It’s not about science, but about pseudoscience and mythical misinterpretations of physics and quantum theory. It says things that are simply not true and misrepresents them as indisputable scientific facts. The film features interviews with patients, with non-scientists, and with a veritable Who’s Who roster of infamous fringe scientists like Rupert Sheldrake and Dean Radin. But it doesn’t offer a single word of comment by any mainstream scientist or by the many skeptics who have examined the “evidence” for so-called energy medicine and found it pathetically inadequate. It doesn’t even acknowledge that dissent is possible.

I’m going to give this movie more attention than it deserves. It wouldn’t merit taking seriously if it weren’t for the fact that legions of energy medicine practitioners are promoting these same false ideas to justify bogus treatments, relieve customers of their hard-earned money, and sometimes even contribute to a premature demise by convincing patients that lifesaving science-based treatments are unnecessary. For that reason alone, the movie’s claims demand a skeptical rebuttal.

The concept of energy healing is vague and incoherent, so poorly thought out that it is nearly impossible to explain. It involves quantum holistic energy fields that somehow hold all kinds of information, that unite us with everything in the universe but also somehow govern the functioning of our individual bodies, and that respond to thought and intention. “The Field” encompasses the entire universe, but there is also a hierarchy of smaller fields for our bodies and for each of our limbs and organs. There is no hypothesis to explain how our thoughts know what to do to which field, much less how they might actually do it. It also has something to do with morphogenetic influences and with static scalar waves. Trying to make sense out of all this is as unedifying as trying to make sense out of a schizophrenic’s word salad. One interviewee says

We’re not in this field, we are this field… we’re denser, we’re lighter in between.

What does this even mean?

There are so many things wrong with the film that it’s hard to know where to start. The testimonials are as good a place as any.

  1. A 5-year-old with cerebral palsy was allegedly healed by “reconnective healing” by a chiropractor who is shown waving his hands a few inches away from the child’s body. Problem: There was no medical evaluation before and after to determine whether anything had objectively changed, and video of the child after treatment shows that his gait is still abnormal.
  2. After a motorcycle accident, a woman was told there was a possibility her injured leg might require amputation. She visualized her immune system and rallied it with her thoughts, claiming she could feel the healing happening. The idea for trying this came to her directly through a noetic process: she just knew intuitively that her mind was important to her body. Problem: There is no reason to think this woman would not have healed just as well without any imaginative mumbo-jumbo. Injuries usually do heal.
  3. A woman who wanted children was diagnosed with prolactinoma, a brain tumor that causes infertility. She believed that she had somehow created the tumor. She refused drugs and surgery and relied on neurolinguistic programming (NLP), a discredited psychological therapy. It helped her discover that deep down some part of her unconscious did not want to be a mother. She let go of that, and let go of the anger against her tumor. She realized that having a tumor had taken her on a journey she would not have taken otherwise, and she liked herself better. So the tumor wasn’t totally bad — what if it had a purpose for being here? She gave it permission to stay for the rest of her life, and 6 months later her prolactin blood tests were normal. Her doctor said “This can only mean one thing: your tumor has gone.” He didn’t bother confirming that with follow-up imaging studies. Problem: A change in hormone levels is not proof that the tumor had resolved. Prolactinomas have been known to spontaneously stop producing excess prolactin, to infarct, and to otherwise resolve without treatment. Microadenomas frequently shrink or disappear and spontaneous regression has been observed even in macro-adenomas. So there’s no reason to attribute her improvement to anything she did.
  4. A woman was so impaired by chronic fatigue syndrome and fibromyalgia that her husband had to feed and carry her. A Nutri-Energetics System (NES) practitioner found that she was “allergic to almost all foods” and that her energy fields were weak, with distortions in the body field. For 6 months she took drops that the practitioner had imprinted with an information pattern, and she was cured. Hard to believe even if you are the White Queen and have practiced believing six impossible things before breakfast.
  5. Former astronaut Edgar Mitchell (who is notorious for believing in strange things like UFOs and ESP) had an MRI that showed an “irregularity” on one kidney. His doctors wanted to do a biopsy, but he refused. Instead he was treated by a teenage intuitive healer, Adam Dreamhealer. Adam’s healing ability developed after a vision directed him to go to the forest where he met a big black bird. The bird imparted complex information to him from the field of information. Adam can look at a photograph and perceive a holographic image of the body and see where energy flow is blocked, indicating illness or injury; then he clears these blockages using only his intention to heal. On follow-up tests 6 months later, Mitchell’s kidney irregularity had disappeared. Unlike all the other fields and energies known to physics, the healing energy fields are undetectable by scientific instruments and do not diminish with distance according to the inverse square law: in this case the healer was in Vancouver, BC, and the patient stayed in Florida. Problem: We have no way of knowing what the “irregularity” was. Could it have been an insignificant lesion that was likely to resolve on its own? Could it have been some kind of imaging artifact? Even if it was kidney cancer, that is a disease with a known propensity for spontaneous remission.

The plural of anecdote is not data: all these testimonials add up to no evidence at all. They are not properly documented and have other natural explanations. And they are a mish-mash of different techniques with no commonalities and no coherent explanatory mechanism. If healing can occur across thousands of miles by intention, why would the chiropractor need to wave his hands over the patient and why would the nutritionist have to administer information via drops? Do they ever try to compare these different energy healing methods to see if one is superior to another? Or to try to investigate their parameters or study what features they have in common? Of course not. Scientists would do that, but this isn’t science.

The movie claims that there are amazing healings taking place all the time that mainstream medicine can’t explain. In fact, medical science has not tried to explain them because it hasn’t seen any credible evidence that there is anything to explain. These alleged healings are poorly documented and/or have other natural explanations. True, conventional medicine can’t explain everything; but neither can these guys. Their “explanation” amounts to a confused, untestable hypothesis that a quantum holistic information-containing energy field can do strange and miraculous things. It pretends to explain everything but it actually explains nothing.

They try to make their belief system sound like science. It isn’t. They get the science spectacularly wrong, using words like “quantum,” “field,” and “energy” in nonscientific ways. Physicist Eugenie Mielczarek recently educated Science-Based Medicine readers about fields, alternative medicine and physics. She explained that

Studies of equations for these forces and the enumeration of the strength of their fields underlie our current technology. When energy fields are used as a medium for conveying information, scientists ask and answer the following key questions: How large is the signal? What is the transmitter located in the source, and what and where is the receiver? How can the device be tuned and detuned? Lastly, how can one replicate this by a device to be used for medical intervention?

The “scientists” of energy medicine don’t even ask such questions, much less answer them. Their “field” concept has no real explanatory power. For instance, they say that we don’t have a full understanding of how wound healing occurs. In fact, we do know plenty of scientific details about the healing process, while they offer no details about how the “field” might cause healing. They just offer vague generalities that “information” is somehow transmitted to the body. The information field is apparently all-knowing and all-powerful, like God. This is essentially a version of the “God of the gaps” excuse of creationists. If you don’t understand something, you just claim God or The Field did it — somehow.

Many of their arguments are versions of the logical fallacies “argument from ignorance” and “argument from personal incredulity.” How could the body know to heal itself? They find it inconceivable that the entire gamut of physiology and human behavior could be explained by a physical brain and nervous system. They claim that the coherence of neuron firing is faster than the ability of the cells to communicate and that this proves that the brain is communicating at a higher level than is possible through physical factors. They can’t imagine how molecules could find each other in the cell for a chemical reaction to occur. They say we can’t explain how the body maintains homeostasis or how DNA guides development of the embryo unless we realize that the body field turns the knobs. They say we may understand how cells work but we don’t understand how they talk to each other and how they deal with information. They say the coordinated turning of a flock of birds could only occur with the help of fields that transfer information with no time delay.

Scientists are working on these problems, have made considerable progress, and are confident that the remaining mysteries are ultimately explainable. Believers in energy healing don’t want to work on trying to explain the mysteries: they are content with the pseudo-explanation of invoking “The Field.”

Intention, belief — can these factors influence healing? They fall back on the “you create your own reality” myth. “If you think you have an incurable disease, you are right. If you think your problem is curable, then you are also right.” This is nothing but wishful thinking.

They make many statements that are distorted misrepresentations of scientific facts or are simply false. Here are some examples:

  • You can read the mind with EEG or magnetoencephalography.
  • The brain is not the central repository of information.
  • DNA doesn’t explain much. Chimps and humans have similar DNA, so that couldn’t explain the difference between them. The explanation is the morphogenetic field that informs which parts of DNA the body will access for its development.
  • Genes do not control our biology.
  • Children adopted into families with a genetic tendency to cancer will have the same risk of cancer.
  • Beliefs and attitudes shape cancer, not genetics.
  • Chemotherapy only works 9% of the time, and works only if you believe in it.
  • “Modern physics understands that it is not matter but mind or spirit [defined as intelligent energy fields] which is primary.”
  • The body can’t distinguish between action and thought.
  • A belief can override biology.
  • Epigenetics is proof that DNA doesn’t matter. It can cause 30,000 different variations of each gene, so we have unlimited potential.
  • Properties like memory are diffuse throughout our brain and we access them from the field. Memory might not exist in the brain at all – it might be somewhere out in the field.
  • Illness is just a lack in the information system. Disease is scrambled information.
  • Matter is compressed energy.
  • The acupuncture system is a system of information flow in the body itself, arranged in a certain order, that communicates in a certain direction.
  • Thought field therapy instantly healed 100% of cases of PTSD in Kosovo.
  • We have a resonant frequency and coherence is its natural state.
  • A scientist has shown that when you start using reconnective healing, enough excess free thermodynamic energy is released that it could raise the room temperature over 300 degrees C. But our temperature doesn’t rise because we’re accessing something new and different.
  • By changing your mind, you change your biology and your genetics.
  • The heart is a functional brain. It may be the master organ for imprinting information into the holographic body field.

Edgar Mitchell, the astronaut, tells us that it has been proven in the lab that intention has physical effects. In one study, spouses of cancer patients got compassionate intention training. When their partners sent loving intentions, the patients’ physiology showed changes. They put the subjects in different rooms and shielded the rooms and thought they had ruled out conventional explanations; but they allowed the receiver to watch the sender on a video monitor, so they didn’t rule out subtle visible signals from body language. Another study allegedly showed that the heart has precognition and responds before an anticipated good or bad picture can register on the brain and even before it is randomly selected by a computer.

Ioannidis has shown us that most published research findings are false; and energy medicine research consists mainly of poorly designed, poorly controlled, isolated demonstrations like these that have low prior probability and have not merited attempts at replication by less credulous researchers. Real science gradually builds an edifice of experiments that confirm each other and achieve progress and fuller understanding. Energy medicine research is usually hit-and-run. They find an apparent phenomenon and instead of checking for flaws and seeing if it can be falsified, instead of trying to better define it and study its properties, they quickly move on to another kind of experiment to demonstrate another phenomenon. The totality of their research is an unconnected mishmash that proves nothing and that has resulted in no progress.

They say that 1/3 of all healings (drugs, surgery, etc.) have nothing to do with the treatment but are due to the placebo effect. That we could cut health care costs by exactly 1/3 by just using the placebo effect. That an inert substance is somehow able to manage a whole cascade of responses in a complex system to target the liver or the kidney: it’s a great mystery. That what medicine calls the placebo effect is really a phenomenon of energy fields.

This is a complete misunderstanding of what science has learned about placebos. It’s an absurd distortion of the fact that in a controlled study, 1/3 of the patients in the placebo control group typically report improvement. That improvement is mostly due to factors like regression to the mean and the natural course of the disease, and when those other factors are controlled for by adding a no-treatment group, most of the apparent placebo response disappears. And while placebos may reduce pain perception, they have never cured cancer or pneumonia.

Mitchell asks us to walk into any cathedral and feel the palpable experience of awe and reverence. He says we have this experience because for hundreds of years the people going in have been in such a state of mind that the quantum emissions from the body-brain were emitted, absorbed into the cathedral, and now are being transmitted back to us. Yeah, sure. Or maybe it’s angels. It couldn’t possibly have anything to do with any natural explanations like psychology, conditioning, suggestion, environmental influences, sensory effects, aesthetic responsiveness, and expectation, could it?

They suggest one possible mechanism for our body’s connection to the field: the biophoton. Biophotons are a random by-product of cellular metabolism; they can only be detected by powerful photomultipliers. Energy healing advocates claim that biophotons create some kind of dynamic coherent web of light within our bodies. But how could they measure that and what would it even mean? They think this web might be regulating the body’s metabolism, since molecules can’t regulate themselves. But it’s not plausible that these ultraweak photons could have any significant effect, or that a whole-body coherent web could result; and it’s even less plausible that it could carry information.

A good rule of thumb is to never accept any new claim without first asking who disagrees with it and why. The movie doesn’t ask such questions. These people are not seeking the truth: they are certain that they already know the truth and they are only seeking to persuade others to accept their belief system. The Living Matrix made my brain hurt. It was only worth watching as an appalling demonstration of the human capacity for self-deception and as a reminder of how badly our error-prone human brains need the discipline of rigorous science and critical thinking.

The movie claims that informational medicine is going to be the future of medicine. Yes, it is; but it’s going to be real information from advances in fields like neurophysiology and genomics. It’s not going to be mystical information transmitted by thought and intention and quantum holistic flapdoodle.


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New Earths Hidden In The Data?

In the Hunt for Planets, Who Owns the Data?, NY Times

"On Tuesday, astronomers operating NASA's Kepler spacecraft will release a list of about 350 stars newly suspected of harboring planets, including five systems with multiple candidate planets. That data could dramatically swell the inventory of alien worlds, which now stands at 461, none of them habitable by the likes of us. ... But a lot of attention has been paid in astronomical circles over the past few months to what the Kepler team will not be saying. By agreement with NASA, the team is holding back data on its 400 brightest and best planet candidates, which the astronomers intend to observe themselves over abusy summer. ... The result has been a shift in the balance between the duty of a scientist to wring every last drop of truth and credibility out of the data he or she might have spent years gathering and the rights of the rest of us to know what our tax dollars have discovered."

Cool Cars: 1982 Excalibur Series IV

Yesterday's blog entry contained a link to a story about Brooks Stevens by Richard Langworth. One of the Stevens' designs that Langworth mentioned was the Excalibur neoclassic, which recently popped up on Hemmings.com. This 1982 Excalibur Series IV just so happens to come from the same year of La

Introduction to Yoga – Twists

There are many benefits to adding twists as part of a well-rounded yoga practice including relief of back pain and pressure, improved digestion, stretching and strengthening of the spine, and many others depending on the pose. If you have problems with your lower back or sacroiliac joints, do

Thoughts on How Digital Pathology Will Usher in New Era of Personalized Medicine

Dr. Joseph Krueger over at his Instablog has some interesting comments concerning digital pathology and personalized medicine. 

He writes "This new technology, whole slide imaging, or digital pathology, is moving pathology closer towards an electronic science. Radiologists have worked exclusively from digital media for years, but pathology is just now moving in this direction. The entire tissue section on the glass slide is scanned in a high-throughput scanner, made by established digital pathology companies like Aperio, Bioimagene, Zeiss, and Hamamatsu, or newer entrants with even faster scanning technologies like Leica, Philips, and GE’s Omnyx subsidiary. The pathologist can now read and diagnose from a computer screen, eliminating the need to ship glass slides around during clinical trials. More importantly, the pathologist can run computer image analysis across the tissue section, generating quantitative information highly useful in the development of a companion diagnostics program. This methodology not only speeds up the pathological assessment process, reducing cost, it provides the means for a rapid and inexpensive companion diagnostic based on the “gold standard” assessment of pathology."

Who could disagree with that?  I think Dr. Krueger touches on one of the many values of digital pathology; the power of image analysis with a digital data set, a real advantage over analog review alone. 

The post provides interesting background and further discussion that is worth checking out:

Digital Pathology Will Usher in New Era of Personalized Medicine

NASA Layoff Update

No NASA employees will lose jobs in Constellation cuts, space agency confirms, Huntsville Times

"NASA Headquarters in Washington confirmed today that no government employees here will lose their jobs in current cuts being made in the Constellation rocket program."

Space Policy Fight May Have No Winners, Aviation Week

"Bolden says "most of these reductions will be implemented via reductions in workforce" in the weeks ahead, "beginning immediately" and totaling an estimated "30-60% of the current population, or 2,500-5,000, for the balance of the year."

Job fairs, workshop planned for space shuttle workers, Florida Today

"Two upcoming job fairs and a workshop aim to assist aerospace professionals whose jobs could end with the shuttle program, and those already out of work."

Mr. Hayward Goes To Washington | The Intersection

The Oil Drum has posted and linked (pdf) to the letter sent by the Energy and Commerce Committee to BP CEO Tony Hayward in preparation for his upcoming testimony regarding concerns over risky practices related to the oil spill. It begins:
Dear Mr. Hayward: We are looking forward to your testimony before the Subcommittee on Oversight and Investigations on Thursday, June 17, 2010, about the causes of the blowout ofthe Macondo well and the ongoing oil spill disaster in the Gulf of Mexico. As you prepare for this testimony, we want to share with you some of the results of the Committee's investigation and advise you of issues you should be prepared to address. The Committee's investigation is raising serious questions about the decisions made by BP in the days and hours before the explosion on the Deepwater Horizon. On April 15, five days before the explosion, BP's drilling engineer called Macondo a "nightmare well." In spite of the well's difficulties, BP appears to have made multiple decisions for economic reasons that increased the danger of a catastrophic well failure. In several instances, these decisions appear to violate industry guidelines and were made despite warnings from BP's own personnel and its contractors. In ...


Water Worlds

Research Suggests Water Content Of Moon Interior Underestimated

"NASA-funded scientists estimate from recent research that the volume of water molecules locked inside minerals in the Moon's interior could exceed the amount of water in the Great Lakes here on Earth."

New CU-Boulder Study Indicates An Ancient Ocean May have Covered One-Third of Mars

"Collectively, these results support the existing theories regarding the extent and formation time of an ancient ocean on Mars and imply the surface conditions during the time probably allowed the occurrence of a global and active hydrosphere integrating valley networks, deltas and a vast ocean as major components of an Earth-like hydrologic cycle," Di Achille and Hynek wrote in Nature Geoscience."

Sen. Nelson Attempts To Formulate a Compromise

Nelson maps a road forward on space without Constellation, Orlando Sentinel

"Although Nelson mentions Ares I and Orion's contracts and assets - the first-phase rocket and crew capsule, respectively, of Constellation -- he does not talk about the program at all. Gone too is any mention of a vigorous test flight program for which Nelson recently requested $726 million. Nelson's spokesman, Dan McLaughlin, said that upon reflection lawmakers decided that it was up to "NASA as to how to get started on HLV as soon as possible." Nelson's approach appears to be an attempt at compromise with critics of the president's plans who have attacked the proposals as a "road to nowhere" that cedes U.S. leadership in space."

Letter From Sen. Nelson to Sen. Mikulski Regarding FY 2011 NASA Budget

"Thank you for your letter of February 16, 2010, outlining your principles for drafting the fiscal year 2011 funding bill for NASA. I share fully your sentiment that our committees must work together to define the best path forward for America's space program. Over the last four months, I have been studying the President's budget request, as well as various alternative proposals, in determining how we can best move ahead to the next era of human space flight. I write today to share with you some of the key elements that have emerged from that review, including discussions with Chairman Rockefeller, Ranking Member Hutchison, Senator Vitter, and other members of our Committee, which will form the bipartisan foundation of a NASA authorization bill."