Hamamatsu – Redefining the art of whole-slide imaging

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Virtual Microscopy / NanoZoomer - Products - NanoZoomer-XR 


Nanozoomer-xrHassle-free

  • Perfect scans at a touch of a button. After slides are loaded, running a batch of slides is virtually hands-free.
  • Scanning profiles can be set up for multiple types and/or users.
  • New viewer software, NDP.View2, speeds and simplifies slide viewing.

Error-free

  • Robust mechanics and optics keep the NanoZoomer error-free and stable.
  • NanoZoomer-XR optimizes scanning conditions to ensure users get the best digital images whenever they scan.

Blur-free

  • Dynamic Pre-Focusing technology (patent pending) maintains a sharp focus on the entire specimen during scanning resulting in crisp and clear images.
  • Slides are automatically evaluated for image quality and re-scanned as needed.

The new NanoZoomer-XR

  • Minimizes work load and speeds slide scanning time by automatically and continuously scanning up to 320 slides per batch.
  • Converts, in as little as 30 seconds, a 15 mm x 15 mm area on a glass slide into an outstanding color image.
  • Scans various types of slides including HE-stained and cytology samples.
  • Features a true multi-Z level acquisition for improved accuracy and viewing of thick specimens.
  • An add-on module for fluorescence scanningwill be available soon.

Learn more 

 

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Simagis Digital Pathology Servers Now Support Leica Microsystems Scanners

 Simagislive

HOUSTON--(BUSINESS WIRE)--Smart Imaging Technologies has signed a license agreement with Leica Microsystems and added support for Leica Slide Scanners to Simagis Live Digital Pathology Servers.

“We are committed to our mission of providing interoperability and integration in the fragmented and rapidly evolving space of Digital Pathology. We are happy to assure our clients and partners that they can continue business as usual, regardless of the outcome of technology consolidation between merging vendors”

Simagis Live Digital Pathology servers already support digital slides from most manufacturers of microscopy slide scanners including Aperio, the market leader soon to be acquired by Leica Microsystems. Adding Leica to the list of supported scanners provides pathology community with digital platform that handles slides from both merging companies and delivers unified solution that works with any vendor, now and in the future.

“We are committed to our mission of providing interoperability and integration in the fragmented and rapidly evolving space of Digital Pathology. We are happy to assure our clients and partners that they can continue business as usual, regardless of the outcome of technology consolidation between merging vendors,” said CEO of the Smart Imaging Technologies.

About Smart Imaging Technologies

We provide digital slide servers, image analysis applications and cloud hosting services that make Digital Pathology affordable for practice of any size. With our technology users can easily create, annotate, share and analyze digital slides from any web browser. Our servers support most digital slide formats and can be easily integrated with various scanners for a single click slide upload. Our products can be custom branded and deployed on premises or the cloud to deliver unified digital pathology solutions across geographic boundaries. For details about technology and solutions manufacturers and integrators may contact support@simagis.us. End users can learn more and sign-up for free web service at web-pathology.net.

Contacts

Smart Imaging Technologies

Evgenia Harris, 713-589-3500

 

 

 

 

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Free Webinar: Analyzing IHC Images Using Image-Pro Premier for Research Pathology

Free Webinar: Analyzing IHC Images Using Image-Pro Premier for Research Pathology

September 11, 2012 1pm-2pm EDT Register

Media Cybernetics invites you to attend the free webinar, “Analyzing IHC Images Using Image-Pro Premier for Research Pathology”, Tuesday, September 11, 2012  at 1pm-2pm EDT. 

The Webinar will introduce the features available within Image-Pro Premier image analysis software to set up and create your own image analysis protocols for the analysis of IHC stained samples.  Examples using IHC stained tissue and nuclei will be demonstrated during the webinar.       

Attendees will learn how to: 

  • Evaluate image quality
  • Apply background correction to correct for uneven illumination when required
  • Use image processing techniques to enhance segmentation as related to IHC samples
  • Set up Count/Size for analyzing single or multiple stains
  • Introduce Smart Segmentation, which enables the segmentation of difficult-to-analyze features
  • Configure Data Collector to accumulate data from multiple images


Register

About the Presenter: 
Jeff Knipe is an Image Analysis Trainer with Media Cybernetics. Jeff has extensive hands-on experience in scientific image processing and analysis of both biological and industrial imaging applications.

 

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Meaningful Use Stage 2: Are You Ready?

Final stage 2 MU rule offers healthcare providers some compromises and EHR tweaks, and also requires greater patient engagement.

By Nicole Lewis,  InformationWeek 
September 04, 2012
URL: http://www.informationweek.com/healthcare/policy/meaningful-use-stage-2-are-you-ready/240006670 


InformationWeek-HealthcareUnder the Meaningful Use stage 2 final rule, eligible professionals (EPs) working in a hospital can apply for incentive payments if they can demonstrate that they've acquired a certified electronic health record (EHR), and are using it in lieu of the hospital's EHR. To qualify, EPs must show that they implemented the EHR and maintain the system, including supporting hardware and interfaces needed to achieve meaningful use, without receiving reimbursements from the hospital.

"We know that there are EPs who work within certain areas or unit of hospitals--for example, neonatal care--but use entirely separate systems, and this application process will allow those EPs to ask for a redetermination of their hospital-based status based on those factors," a spokesman for the Centers for Medicare & Medicaid Services (CMS) told InformationWeek HealthcareAdTech Ad

Expanding the criteria for EPs to apply for incentive payments is one of the many changes incorporated into the Meaningful Use stage 2 final rule, a 672-page document recently published by CMS.


Ipad_HC_lg2Many of the changes address real-world concerns that healthcare providers face as they prepare their systems to meet Meaningful Use criteria, said Dr. Farzad Mostashari, National Coordinator for Health IT at the Office of the National Coordinator for Health Information Technology.

[ For more on Meaningful Use stage 2, see Meaningful Use Stage 2 Rules Finalized. ]

"What you will see [in the stage 2 final rule] is some compromises frankly between the aspirational goals and the realities of where the market is and the pressures on providers and vendors in terms of timeline," Mostashari said during a webcast August 24 hosted by the National eHealth Collaborative.


Meaningful useExtending the timeline to meet Meaningful Use stage 1 deadlines is one area of compromise. In the stage 1 Meaningful Use regulations, CMS established a timeline that required providers to progress to stage 2 criteria after completing two years in the incentive program. This original timeline would have required Medicare providers who first demonstrated Meaningful Use in 2011 to meet the stage 2 criteria in 2013.

However, CMS pushed back the timeline by one year. The earliest that the stage 2 criteria will be effective is fiscal year 2014 for eligible hospitals and critical access hospitals (CAH), or calendar year 2014 for EPs.

Hospitals and physicians must also keep in mind that many of the stage 1 requirements that have been carried forward to stage 2 have a concomitant rise in threshold levels, according to Rob Anthony, a health specialist in the Office of eHealth Standards and Services at CMS.

Explaining the new requirements during the webcast, Anthony said health providers must show that more than 50% of prescriptions are completed through electronic prescribing, up from the stage 1 threshold of 40%. Reporting of demographic information, vital signs, and smoking status has moved from 50% in stage 1 to more than 80% in stage 2. CMS also raised the requirement for reporting instances of clinical decision support and intervention from one to five. These interventions include reporting on drug-to-drug interactions and drug allergy interaction alerts.

Turning to clinical quality measure (CQM) reporting in stage 2, beginning in 2014 EPs must report on nine out of 64 total CQMs, and eligible hospitals and CAHs must report on 16 out of 29 total CQMs.

In addition, all providers must select CQMs from at least three of the six key healthcare policy domains recommended by the Department of Health and Human Services' National Quality Strategy. These are: 1. Patient and Family Engagement; 2. Patient Safety; 3. Care Coordination; 4. Population and Public Health; 5. Efficient Use of Healthcare Resources; and 6. Clinical Processes/Effectiveness.

Stage 2 will also focus more intensely on facilitating patients' access to their records, and replaces stage 1 objectives that call for clinicians to provide electronic copies of health information or discharge instructions, with the stage 2 objectives that allow patients to access their health information online.

For EPs, stage 2 requires that patients have the ability to view online, download, and transmit their health information within four business days of the information being available to the EP. Eligible hospitals and CAHs are required to provide patients the ability to view online, download, and transmit their health information within 36 hours after discharge from the hospital.

Patients must also do their part in stage 2, which calls for more than 5% of patients to send secure messages to their EP, and more than 5% of patients to access their health information online. CMS is introducing exclusions based on broadband availability in the provider's county.

Stage 2 also facilitates batch reporting. Starting in 2014, groups can submit attestation information for all of their individual EPs in one file for upload to the attestation system, rather than having each EP individually enter data.

In the stage 2 criteria, the feds emphasized the need to exchange health information between providers to improve care coordination for patients. One of the core objectives requires EPs, eligible hospitals, and CAHs who transition or refer a patient to another care setting or provider to furnish a summary of care record for more than 50% of those transitions of care and referrals.

Additionally, there are new requirements for the electronic exchange of summary of care documents: EPs, eligible hospitals, and CAHs that transition or refer their patient to another care setting or provider must electronically provide a summary of care record for more than 10% of transitions and referrals.

The EP, eligible hospital, or CAH that transitions or refers a patient to another care setting or provider must either: a) conduct one or more successful electronic exchanges of a summary of care record with a recipient using technology that was designed by a different EHR developer than the sender's, or b) conduct one or more successful tests with the CMS-designated test EHR during the EHR reporting period.

InformationWeek Healthcare brought together eight top IT execs to discuss BYOD, Meaningful Use, accountable care, and other contentious issues. Also in the new, all-digital CIO Roundtable issue: Why use IT systems to help cut medical costs if physicians ignore the cost of the care they provide? (Free with registration.)


 

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Educational Flow Cytometry Video (Humor)

One things for sure, you will not hear the words: multineage dysplasia, macrophages, bcl-6, S-phase, monoclonal, Burkitt’s, Rituxin, MDS, AML, translocated genes, APL, Auer rods, all-trans retinoic acid, DIC, CD45 or CD117 all in the same music video again ever.

From DrdoubleB who also produced among other educational pathology videos, Dynamite Case about an interesting surgical pathology case at the University of Florida.

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Actress Emma Stone Loves The Morbid Anatomy Library: "Vogue UK" and the "Herald Scotland," 2012

... How does [starlett Emma Stone's] obsession with mortality and death manifest itself? "By going to places like the Morbid Anatomy Library [in Brooklyn, New York] the other day, looking at little foetal pigs in jars. I have an interest in death. Obviously not constantly, but on a daily basis.

"There's an awareness of mortality, I think, that makes you live much more presently. There's something oddly comforting about death. Not dying. Dying, I'm terrified of, but death -" She pauses. "Sorry, am I getting really serious? Come on, we're in Cancun!"

-- "Emma Stone gets caught in Spider-Man's web," Herald Scotland, June 2012 (full article here)

A few months back, the Morbid Anatomy Library was graced with a visit by lovely, young and--apparently--morbidly inclined starlett Emma Stone, star of Easy A, Crazy, Stupid, Love, The Help and, most recently, The Amazing Spiderman. She was trailed on this wine-soaked visit by reporter Alexa Chung of Vogue UK, and we spent a lovely rainy hour or two paging through some of my favorite books, poking around the taxidermy collection, and discussing our shared love for the macabre. The trip resulted not only in the expected article for Vogue UK--entitled "The Crazy Cool of Emma Stone," in the August 2012 issue--but also the piece from the Herlad Scotland quoted above.

You can read the entire Vogue UK article--in which you will  learn more about Stone's favorite books and artifacts in the collection, among other things--by clicking here, and the entire Herald Scotland piece by clicking here. Thanks so much to Jo Hanks for stumbling across the article, and for the donation of her very own issue of Vogue UK to this worthy cause! And thanks to good friend Eric Huang for alerting me.

Also, please feel free to come visit the library and see the collection for yourself during our next set of open hours this Saturday, September 15th, from 1-6. Details and directions here.

Photos of The Library by Shannon Taggart

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Leopold Koss, MD Passes Away


Obituary_505366cb43bbeLeo Koss 00Leopold G. Koss MD, passed away at his home in New York City, September 11, 2012. Born in Gdansk, Poland in 1920, Leo (as he was known to all who loved him) miraculously survived the Holocaust and became a refugee in Switzerland where he finished the medical studies he had started in Vienna before the War and continued in Brussels and Montpellier. When he arrived in New York in 1947 with his wife and baby son and $20 in his pocket, he spoke no English. Yet, with his prodigious intellect and devotion to science, he rose to the top of his field in his adopted country. He was a pioneer in the field of Cytopathology and was the author of the seminal book on the subject, "Diagnostic Cytology and It's Histopathologic Bases," which appeared in five editions and was recently translated into Chinese. Over his long career, he also wrote numerous other medical books and hundreds of scientific papers. Dr. Koss was chairman emeritus of the Department of Pathology at Montefiore Hospital and the Albert Einstein College of Medicine, which was renamed in his honor. He will be remembered by all who knew him as a man of great intelligence, culture and wit. He was a gifted storyteller who could, depending on his audience, recite French love poetry, sing baudy ditties or quote Caesar's Gallic Wars in the original Latin He is survived by his three sons, Michael, Andrew, and Richard as well as 4 grandchildren. A memorial service will be held at Riverside Memorial Chapel on October 26 at 2 pm. In lieu of flowers, donations can be made in his honor to the Visiting Nurse Service of NYC whose dedicated employees cared for him at the end of his life with unquestioned respect and love.

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Free Webinar: What are the Technical Barriers to the Adoption of Digital Pathology

September 19, 2012 | 11:00AM - 12:00PM EST


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This webinar is the second in the DPA, Association of Pathology Informatics (API), and CAP Today's series of one-hour webinars focusing on the barriers to adoption of digital pathology. Technical Barriers to the Adoption of Digital Pathology will feature Sean Costello, Head of Product Management - Digital Pathology at Leica Microsystems, and Kim Dickinson, Senior Medical Director - Integrated Oncology at US Labs. The webinar will be moderated by Robert McGonnagle, publisher of CAP Today.

All of the webinars in this series serve as a bridge between the recent, comprehensive article about regulatory aspects of digital pathology that appeared in CAP Today (see: Regulators Scanning the Digital Scanners) and the two most important, national conferences focusing on digital pathology, Pathology Visions 2012, the annual conference of the DPA, to be held October 28-31, 2012 and Pathology Informatics 2012 to be held October 9 – 12, 2012 in Chicago.

There is no charge to participate in the webinar, however advance registration is required.


Webinar_Register

ARE YOU A MEMBER OF API, CAP, OR ANOTHER ASSOCIATION?

Members of AACR, ACVP, API, APF, ASCO, ASCAP, CAP, CSP, NSH, STP, and USCAP receive registration discount for Pathology Visions 2012.

DPA members aren’t the only association members that will have the opportunity to receive a discounted registration for Pathology Visions 2012. A special affiliate rate has been negotiated for members of the following organizations: AACR, ACVP, API, APF, ASCO, ASCAP, CAP, CSP, NSH, STP, and USCAP. The Affiliated Association & Society registration is all-inclusive.

If you are a member of any of these associations look for an e-mail from your association with registration instructions and a discount code or contact the DPA staff for more information.

Register Now Button

 

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Police hope DNA will help solve cold cases

For 30 years, Peggy Sue Houser was listed as a missing person. For nearly the same time, the Piqua womans unidentified corpse lay buried in Hillsborough County, Fla.

Last year, DNA brought the two cases together, something that may happen more frequently as local detectives submit genetic samples to the Center for Human Identification at the University of North Texas Health Science Center.

Funded by a National Institute of Justice grant, the Center is taking DNA samples from law enforcement agencies and coroners offices across the country, in an effort to match missing persons cases with unidentified remains.

Dayton Detective Patricia Tackett, who is assigned to cold cases, said she has collected DNA from the family members of nearly all of the eight open missing person cases she has, even the ones where the missing person has been declared dead.

Just because theyre declared dead doesnt mean weve recovered their bodies, and there are plenty of bodies out there, said Tackett.

Cold cases, by nature, are tough if they were easy cases, they would have been solved, police said. But missing persons cases offer a unique set of challenges. There is no body, no crime scene. Sometimes its not exactly clear when the person disappeared. Often police cant even prove a crime has been committed.

But in recent years, the federal government has taken steps to help match the cases of unidentified bodies estimated at more than 40,000 nationwide to those missing persons.

The National Missing and Unidentified Persons System (NamUs), an online tool administered by the National Institute of Justice, is now fully searchable by the public with databases of information from both missing person cases and those of human remains. NamUS is also administrated at the University of North Texas Health Science Center, like the DNA collection program.

Under the DNA program, law enforcement officials are given free collection kits to obtain the DNA from the close relatives of missing people. The kits are processed at the Center for Human Identification, also for free. The samples are then uploaded into the FBIs Combined DNA Index System (CODIS), so that it can be compared to those of unidentified remains. If relatives are not available, then police sometimes can get a genetic profile from the missing persons property, such as a hairbrush or toothbrush.

The Center claims to have assisted with more than 180 identifications made from Hawaii to New York.

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Police hope DNA will help solve cold cases

Posted in DNA

DNA may help solve cold cases

For 30 years, Peggy Sue Houser was listed as a missing person. For nearly the same time, the Ohio womans unidentified corpse lay buried in Hillsborough County, Fla.

Last year, DNA brought the two cases together, something that may happen more frequently as local detectives submit genetic samples to the Center for Human Identification at the University of North Texas Health Science Center.

Funded by a National Institute of Justice grant, the Center is taking DNA samples from law enforcement agencies and coroners offices across the country, in an effort to match missing persons cases with unidentified remains.

Cold cases, by nature, are tough if they were easy cases, they would have been solved, police said. But missing persons cases offer a unique set of challenges. There is no body, no crime scene. Sometimes its not exactly clear when the person disappeared. Often police cant even prove a crime has been committed.

In Butler County, missing cases involving Alana Laney Gwinner of Fairfield, Katelyn Markham of Fairfield, Ronald Tammen Jr. of Oxford and William DiSilvestro of Hamilton have went unsolved for years.

But in recent years, the federal government has taken steps to help match the cases of unidentified bodies estimated at more than 40,000 nationwide to those missing persons.

The National Missing and Unidentified Persons System (NamUs), an online tool administered by the National Institute of Justice, is now fully searchable by the public with databases of information from both missing person cases and those of human remains. NamUS is also administrated at the University of North Texas Health Science Center, like the DNA collection program.

Under the DNA program, law enforcement officials are given free collection kits to obtain the DNA from the close relatives of missing people. The kits are processed at the Center for Human Identification, also for free. The samples are then uploaded into the FBIs Combined DNA Index System (CODIS), so that it can be compared to those of unidentified remains. If relatives are not available, then police sometimes can get a genetic profile from the missing persons property, such as a hairbrush or toothbrush.

Bill Hagmaier, Executive Director of the International Homicide Investigators Association and a former FBI crime profiler, said the recent changes are long overdue. His group helped develop the DNA initiative a few years back, which he said he wanted to do 20 years ago.

He said the military has long done a far better job of matching bodies to those reported missing in action, just so much more than what were doing for our civilians here.

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DNA may help solve cold cases

Posted in DNA

Regents set to confirm executive director

Regents set to confirm executive director

Center for Teaching Excellence: a program whose goal is to assist faculty with implementing technology-driven course enhancements and advanced features of the electronic course management system.

Source: OU Public Affairs

A new director has been appointed to a program at OU designed to help faculty improve courses with new technology.

Teaching strategies expert Mark Morvant, a professor in the department of chemistry and biochemistry, will be appointed Oct. 1 as the executive director of the Center for Teaching Excellence, as long as the OU Board of Regents approves his appointment at its September meeting, according to an OU press release.

Morvant will work with Michele Eodice, associate provost for academic engagement, to embed writing strategies within disciplines across the campus and increase the use of other high-impact instructional techniques, according to the press release.

I think the future for the University of Oklahoma is very bright I think theres an excitement among the faculty about improving the educational experience for our students, and Im honored to lead our faculty in improving the students education, Morvant said.

In 2006, Morvant began teaching at OU as a chemistry professor and was named assistant chairman of the chemistry and biochemistry department in 2011, according to the press release.

He will step down from teaching for a few years to build the Center for Teaching Excellence program but plans on eventually returning to limited teaching on a routine basis, Morvant said.

Morvant also will be stepping down from his position as assistant chairman of the chemistry and biochemistry department but will continue to have a faculty appointment in the department, he said.

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Regents set to confirm executive director

Surgery Has a More Profound Effect Than Anesthesia on Brain Pathology and Cognition in Alzheimer’s Animal Model

Newswise PHILADELPHIA A syndrome called post-operative cognitive decline has been coined to refer to the commonly reported loss of cognitive abilities, usually in older adults, in the days to weeks after surgery. In fact, some patients time the onset of their Alzheimers disease symptoms from a surgical procedure. Exactly how the trio of anesthesia, surgery, and dementia interact is clinically inconclusive, yet of great concern to patients, their families and physicians.

A year ago, researchers at the Perelman School of Medicine at the University of Pennsylvania reported that Alzheimer's pathology, as reflected by cerebral spinal fluid biomarkers, might be increased in patients after surgery and anesthesia. However, it is not clear whether the anesthetic drugs or the surgical procedure itself was responsible. To separate these possibilities, the group turned to a mouse model of Alzheimers disease.

The results, published online this month in the Annals of Surgery, shows that surgery itself, rather than anesthesia, has the more profound impact on a dementia-vulnerable brain.

The team, led by Roderic Eckenhoff, MD, Austin Lamont Professor of Anesthesia, exposed mice with human Alzheimer disease genes, to either anesthesia alone, or anesthesia and an abdominal surgery. The surgery was similar to appendectomy or colectomy, very common procedures in humans. They found that surgery causes a lasting increase in Alzheimers pathology, primarily through a transient activation of brain inflammation. Also, a significant cognitive impairment persisted for at least 14 weeks after surgery compared to controls receiving anesthesia alone. Neither surgery nor anesthesia produced changes in normal non-transgenic animals.

In the mice, there was a clear and persistent decrement in learning and memory caused by surgery as compared with inhalational anesthesia but only in the context of a brain made vulnerable by human Alzheimer-associated transgenes, notes Eckenhoff.

He also notes that at the time of surgery, the AD mice showed no outward symptoms of AD, despite having subtle evidence of ongoing neuropathology. This timeline is analogous to both the age range and cognitive status of many of our patients presenting for a surgical procedure and suggests the window of vulnerability to surgery of the Alzheimers brain extends into this pre-symptomatic period, says Eckenhoff. This period might be analogous to what is now called prodromal AD.

On the other hand, cautions Maryellen Eckenhoff, PhD, a neuroscientist on the team, the brain vulnerability seen in the AD mice may not translate well to people. The AD mice used, like all current mouse models of Alzheimer disease, more closely resemble the situation in familial Alzheimer disease, which constitutes only a small minority of patients. She points out that it is not yet clear whether results from AD mouse models will represent patients who eventually get late-onset, or sporadic Alzheimer disease. These mice are, however, the current standard of choice for screening new drugs and have yielded considerable insight into Alzheimer pathogenesis.

The mechanism linking surgery and the cognitive effects seems to be inflammation. An inflammatory process is well known to occur as a result of surgery, at least outside the central nervous system. How this inflammatory process gains access to the brain, and accelerates AD pathology in a persistent way is still unclear.

Postoperative cognitive decline has not been convincingly demonstrated to persist after three months in most people, and whether it predicts later dementia is still unclear. This study suggests that in the setting of a vulnerable brain, the cognitive deficits after surgery might be irreversible.

However, the finding that inflammation is the underlying mechanism, immediately suggests a strategy for mitigating injury. Human studies will be needed to first confirm these findings and then begin to deploy anti-inflammatory strategies to minimize injury, adds Eckenhoff. As a profession, doctors need to understand the long-term implications of our care, both positive and negative, and do all we can to delay the onset of dementia.

Here is the original post:
Surgery Has a More Profound Effect Than Anesthesia on Brain Pathology and Cognition in Alzheimer's Animal Model

Surgery more profound effect than anesthesia on brain pathology, cognition in Alzheimer’s mice

Public release date: 14-Sep-2012 [ | E-mail | Share ]

Contact: Karen Kreeger karen.kreeger@uphs.upenn.edu 215-349-5658 University of Pennsylvania School of Medicine

PHILADELPHIA A syndrome called "post-operative cognitive decline" has been coined to refer to the commonly reported loss of cognitive abilities, usually in older adults, in the days to weeks after surgery. In fact, some patients time the onset of their Alzheimer's disease symptoms from a surgical procedure. Exactly how the trio of anesthesia, surgery, and dementia interact is clinically inconclusive, yet of great concern to patients, their families and physicians.

A year ago, researchers at the Perelman School of Medicine at the University of Pennsylvania reported that Alzheimer's pathology, as reflected by cerebral spinal fluid biomarkers, might be increased in patients after surgery and anesthesia. However, it is not clear whether the anesthetic drugs or the surgical procedure itself was responsible. To separate these possibilities, the group turned to a mouse model of Alzheimer's disease.

The results, published online this month in the Annals of Surgery, shows that surgery itself, rather than anesthesia, has the more profound impact on a dementia-vulnerable brain.

The team, led by Roderic Eckenhoff, MD, Austin Lamont Professor of Anesthesia, exposed mice with human Alzheimer disease genes, to either anesthesia alone, or anesthesia and an abdominal surgery. The surgery was similar to appendectomy or colectomy, very common procedures in humans. They found that surgery causes a lasting increase in Alzheimer's pathology, primarily through a transient activation of brain inflammation. Also, a significant cognitive impairment persisted for at least 14 weeks after surgery compared to controls receiving anesthesia alone. Neither surgery nor anesthesia produced changes in normal non-transgenic animals.

"In the mice, there was a clear and persistent decrement in learning and memory caused by surgery as compared with inhalational anesthesia but only in the context of a brain made vulnerable by human Alzheimer-associated transgenes," notes Eckenhoff.

He also notes that at the time of surgery, the AD mice showed no outward symptoms of AD, despite having subtle evidence of ongoing neuropathology. "This timeline is analogous to both the age range and cognitive status of many of our patients presenting for a surgical procedure and suggests the window of vulnerability to surgery of the Alzheimer's brain extends into this pre-symptomatic period," says Eckenhoff. This period might be analogous to what is now called prodromal AD.

"On the other hand," cautions Maryellen Eckenhoff, PhD, a neuroscientist on the team, "the brain vulnerability seen in the AD mice may not translate well to people." The AD mice used, like all current mouse models of Alzheimer disease, more closely resemble the situation in familial Alzheimer disease, which constitutes only a small minority of patients. She points out that it is not yet clear whether results from AD mouse models will represent patients who eventually get late-onset, or "sporadic" Alzheimer disease. These mice are, however, the current standard of choice for screening new drugs and have yielded considerable insight into Alzheimer pathogenesis.

The mechanism linking surgery and the cognitive effects seems to be inflammation. An inflammatory process is well known to occur as a result of surgery, at least outside the central nervous system. How this inflammatory process gains access to the brain, and accelerates AD pathology in a persistent way is still unclear.

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Surgery more profound effect than anesthesia on brain pathology, cognition in Alzheimer's mice

People Who Read Nutrition Labels Are Skinnier

Women who read nutrition labels are, on average, eight pounds lighter than those who don't.

Here's a novel approach to weight loss that doesn't require a membership, Zumba headgear, or more than a few seconds of your time: reading nutrition labels.

In the United States, women who pay attention to food labels in a survey by the Centers for Disease Control were found to have an average body mass index 1.49 points lower than their counterparts who didn't, astudy in the journal Agricultural Economicsfound. For an average U.S. female, that's about an eight-pound difference.

The results come from over 25,000 data points gathered from the National Health Interview Survey, an annual poll conducted by the CDC. Of urban populations, 49 percent currently report that they pay attention to food labels. Across the country overall, that figure soars to 74 percent among women, compared to only 58 percent of men. White women who read nutrition labels showed the greatest difference in BMI over their non-reading counterparts, at about 1.76 points.

For better or worse, men -- averaged among all races -- see very little benefit from the extra work. The difference in body-mass index associated with label-reading for a typical American male was just 0.12 points.Still, that counts for something.

While this study can't conclude that this relationship is causal -- people who read food labels more may be more inclined toward healthier lifestyles that actually accounted for the weight loss -- there's something to be said for the association. Logically, informed consumers make better choices, and we've seen how food labeling can be an effective way to change consumer purchasing habits. So urging people to read nutrition info -- as we face the epidemic that's on pace to see 40 percent of Americans obese within the next 20 years -- is almost certainly in the public interest.

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People Who Read Nutrition Labels Are Skinnier

The risks of antibiotic resistance and consumption: learning with hands-on activities

ScienceDaily (Sep. 14, 2012) An innovative laboratory-based summer project -- Microbiology recipes: antibiotics la carte -- addressing antibiotic resistance and natural antibiotics has been shown to be an effective strategy to increase high school students' awareness of antibiotic resistance and the relevance of rational antibiotic use. In contrast to traditional educational interventions, which mainly rely on large-scale information campaigns, this project's instructional design was devised to take advantage of the acknowledged benefits of laboratory work, by encouraging the participants' active engagement in their learning.

The study is presented by a group of researchers from the University of Porto, Portugal in the latest issue of PLOS ONE.

Microbiology recipes: antibiotics la carte is a one-week long inquiry-based summer project implemented in the scope of Porto's Junior University -- a summer school-based initiative fostered by the University of Porto, which seeks to promote Science & Technology, Arts, Humanities and Sports education amongst elementary and high school students (aged 11 to 17). Each year, the University's Faculties open their doors to approximately 5000 students, who are invited to take part in a wide range of projects designed by university lecturers and implemented by undergraduate and graduate students in a relaxed, but didactically-focused environment.

The widespread of bacterial resistance to antibiotics is a major public health issue that demands concerted educational interventions to raise public awareness and promote judicious antibiotic use. Consistently with this perceived need, several educational programs have been put forth and numerous didactic resources have been developed. However, reliable indicators of the efficacy of most of these resources have not been consistently provided so far. Furthermore, studies reveal that the general public remains unaware of basic aspects related to the modes of action of antibiotics and frequently engage in misinformed behaviors.

Considering that educational programs targeting young people can contribute to a future generation of scientifically literate antibiotic users, the group of researchers from the University of Porto developed, implemented and assessed a hands-on interventional program to raise young people's interest and consciousness about the consequences of antibiotic resistance and foster their sense of self-responsibility in this regard. Maria Joo Fonseca, one of the researchers involved in the study says "we were interested in sparking students' interest, and scaffolding their scientific reasoning about the processes involved in antibiotic resistance and natural antibiotics' activity, by prompting the connection between observable phenomena and the underlying ideas. We found that, by combining diverse activities, ranging from bioinformatics exercises to natural antibiotic testing, it was possible to address misconceptions, improve students' understanding and promote the development of procedural skills." Fernando Tavares, the coordinator of the project, adds "this study evidences the benefits of incorporating hands-on activities in science education programs. We believe that the data gathered illustrate how an informal educational environment such as the one provided by the Junior University can have a measurable and effective impact on our students, and contribute to promote scientific literacy about pressing socio-scientific issues amongst future generations."

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The above story is reprinted from materials provided by IBMC.INEB Associate Laboratory, via AlphaGalileo.

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The risks of antibiotic resistance and consumption: learning with hands-on activities

Research: Hopping DNA supercoils

Artistic impression of the dynamics of DNA supercoils. A person manipulates a long DNA molecule. Loops in the DNA molecule are created by winding up the DNA. For the first time ever, the research by Van Loenhout, Grunt and Dekker revealed how these DNA loops dynamically move along the DNA strand.

If you take hold of a DNA molecule and twist it, this creates 'supercoils', which are a bit like those annoying loops and twists you get in earphone cables. Research carried out by TU Delft, The Netherlands, has found that in the DNA molecule these coils can make their way surprisingly quickly along the length of the DNA. This newly discovered 'hopping' mechanism - which takes places in a matter of milliseconds - could have important biological implications, because cells use the coils to bring specific pieces of DNA into contact with one another. The researchers from Cees Dekker's group at the Kavli Institute of Nanoscience in Delft will be publishing their results in Science this week.

Supercoiling

A DNA molecule in a cell is not simply a loose wire; it is completely wound up in a tangle of loops ('DNA supercoils'). These supercoils in a DNA molecule (see the illustration on the right) are similar to those annoying loops and twists you often get in earphone cables.

In living cells, the DNA supercoils form and unravel and move along the DNA molecule. They are vital to the regulation of DNA activity, in determining which genes are switched on or off for example. One of the ways in which cells use the supercoils is to bring pieces of DNA into contact with one another.

Dynamic

Static images of the DNA supercoils have been studied in detail in the past, but their dynamics remained unknown up till now. PhD student Marijn van Loenhout from the Kavli Institute of Nanoscience at Delft developed a new technique that enabled him to observe how the coils travel along a DNA molecule for the first time. The research was led by Professor Cees Dekker, head of the Bionanoscience Department.

The TU Delft team used magnetic tweezers to stretch out a small section of a DNA molecule and were then able to observe the movement of the DNA coils using fluorescence microscopy (see movies at the website). They succeeded in showing these movements in real time, at the level of the individual DNA molecule.

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Van Loenhout: "We have discovered that the coils can move slowly along the DNA via diffusion. But what we also saw - and this was totally unexpected - that they can 'hop' along relatively long distances (micrometres). In such a movement a loop disappears in one spot, while simultaneously another loop appears in another spot, much further away. This information enables us to test theories about the mechanics of DNA, testing how you tie a knot in DNA, as it were."

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Research: Hopping DNA supercoils

Posted in DNA

Research and Markets: Future Horizons in the Global Infectious Disease Testing Market: Supplier Shares and Sales …

DUBLIN--(BUSINESS WIRE)--

Research and Markets (http://www.researchandmarkets.com/research/dztlbt/future_horizons_in) has announced the addition of the "Future Horizons in the Global Infectious Disease Testing Market: Supplier Shares and Sales Forecasts for 100 Tests by Country" report to their offering.

This comprehensive seven-country report is designed to assist diagnostics industry executives, as well as companies planning to diversify into the dynamic and rapidly expanding microbiology testing market, in evaluating emerging opportunities and developing effective business strategies.

The report provides market segmentation analysis of over 90 diseases and viruses in seven countries, assessment of emerging technologies, review of current instrumentation, as well as strategic profiles of leading suppliers and recent market entrants with innovative technologies and products.

Rationale:

The microbiology testing market is one of the most rapidly growing segments of the in vitro diagnostics industry, and the greatest challenge facing suppliers. Among the main driving forces is continuing spread of AIDS, which remains the world's major health threat and a key factor contributing to the rise of opportunistic infections; threat of bioterrorism; advances in molecular diagnostic technologies; and wider availability of immunosuppressive drugs.

Although for some infections the etiology is still a mystery, while for others the causative microorganisms are present in minute concentrations long before the occurrence of first clinical symptoms, recent advances in genetic engineering and detection technologies are creating exciting opportunities for highly sensitive, specific and cost-effective products.

Geographic Coverage:

- France

- Germany

See the rest here:
Research and Markets: Future Horizons in the Global Infectious Disease Testing Market: Supplier Shares and Sales ...

Research and Markets: Future Horizons in the German Microbiology Market: Supplier Shares and Sales Forecasts for 100 …

DUBLIN--(BUSINESS WIRE)--

Research and Markets (http://www.researchandmarkets.com/research/d23jv9/future_horizons_in) has announced the addition of the "Future Horizons in the German Microbiology Market: Supplier Shares and Sales Forecasts for 100 Infectious Disease Tests by Market Segment" report to their offering.

Highlights:

- Comprehensive 983-page analysis of the German microbiology testing market.

- Major issues pertaining to the German microbiology laboratory practice, as well as key economic, regulatory, demographic, social and technological trends with significant market impact during the next ten years.

- Current scientific views on the definition, epidemiology, and etiology of major infectious diseases and microorganisms.

- Ten-year test volume and sales forecasts for nearly 80 microbiology tests performed in German hospitals, blood banks and commercial laboratories.

- Instrumentation technologies and feature comparison of leading analyzers.

- Sales and market shares of leading suppliers.

- Emerging diagnostic technologies and their potential market applications.

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Research and Markets: Future Horizons in the German Microbiology Market: Supplier Shares and Sales Forecasts for 100 ...

Research and Markets: Future Horizons in the Global Microbiology Market: Supplier Shares and Sales Forecasts for 100 …

DUBLIN--(BUSINESS WIRE)--

Research and Markets (http://www.researchandmarkets.com/research/glb7m3/future_horizons_in) has announced the addition of the "Future Horizons in the Global Microbiology Market: Supplier Shares and Sales Forecasts for 100 Infectious Disease Tests by Country" report to their offering.

This comprehensive seven-country report is designed to assist diagnostics industry executives, as well as companies planning to diversify into the dynamic and rapidly expanding microbiology testing market, in evaluating emerging opportunities and developing effective business strategies.

The report provides market segmentation analysis of over 90 diseases and viruses in seven countries, assessment of emerging technologies, review of current instrumentation, as well as strategic profiles of leading suppliers and recent market entrants with innovative technologies and products.

Rationale

The microbiology testing market is one of the most rapidly growing segments of the in vitro diagnostics industry, and the greatest challenge facing suppliers. Among the main driving forces is continuing spread of AIDS, which remains the world's major health threat and a key factor contributing to the rise of opportunistic infections; threat of bioterrorism; advances in molecular diagnostic technologies; and wider availability of immuno-suppressive drugs.

Although for some infections the etiology is still a mystery, while for others the causative microorganisms are present in minute concentrations long before the occurrence of first clinical symptoms, recent advances in genetic engineering and detection technologies are creating exciting opportunities for highly sensitive, specific and cost-effective products.

Geographic Coverage

- France

- Germany

Read the rest here:
Research and Markets: Future Horizons in the Global Microbiology Market: Supplier Shares and Sales Forecasts for 100 ...