Monthly Archives: May 2020

A girl in New Delhi gets a nasal swab to test for the new coronavirus. A rare Kawasaki diseaselike illness linked to the virus is sickening young people.

By Jennifer Couzin-FrankelMay. 21, 2020 , 4:10 PM

Sciences COVID-19 reporting is supported by the Pulitzer Center.

Three children at one London hospital in mid-April, followed the next day by three at anotherfor Elizabeth Whittaker, a pediatric infectious disease doctor at Imperial College London, those first cases raised an alarm. The youngsters had fevers, rashes, stomach pain, and, in some cases, heart problems, along with blood markers that characterize COVID-19 in adults, including one associated with clotting. But in most, nasal swabs failed to reveal any virus.

I dont understandthey look like they have coronavirus, Whittaker recalls thinking. Doctors nonetheless suspected a link. Within days, a survey turned up 19 additional cases across England, and an alert on 27 April asked doctors to be on the lookout for such symptoms in children. Soon after, dozens more cases surfaced in New York along with smaller clusters elsewhere, bolstering a connection to the pandemic. Reports of children on life support and some deaths put parents on edgeand were especially disheartening after earlier signs that COVID-19 largely spares children from serious illness.

It is another surprise from a virus that hasproffered many, and projects worldwide are gearing up to study it. They are combing the blood and sequencing the genomes of patientsand the virus, if it can be isolated from themto search for clues to what makes some children susceptible and how to head off the worst symptoms. Theres hope that whats learned from young patients might help the many adults in whom COVID-19 also triggers a grievous overreaction of the immune system.

In some respects, Its absolutely not shocking to see this, says Rae Yeung, a rheumatologist and immunologist at the Hospital for Sick Children in Toronto, whose center treated 20 children over the past 3 weeks with similar symptoms.Many pathogens occasionally trigger a similar hyperactive immune response in children, known as Kawasaki disease. Its symptoms vary but include rash, fever, and inflammation in medium-size blood vessels. Children can suffer heart problems. In rare cases, blood pressure plummets and shock sets in.

Doctors disagree on whether the variant linked to COVID-19 is Kawasaki disease or something new, with some experts calling it multisystem inflammatory syndrome in children. But as with Kawasaki disease, most recover with treatment, including steroids and immunoglobulins, which calm the immune system.

In linking the inflammatory syndrome to COVID-19,Were going on more than just a hunch, says Jesse Papenburg, a pediatric infectious disease specialist at Montreal Childrens Hospital, in a city thats seen about 25 children with the condition. Kawasaki disease is rare, ordinarily affecting just one to three in every 10,000 children in Western countries, though its more common in children with Asian ancestry. The spikes recorded so far, in COVID-19 hot spots like northern Italy and New York City, track the novel coronavirus march around the world. And although a minority of these children test positive for SARS-CoV-2, a studypublished inThe Lancetby a team in Bergamo, Italy, reported that eight of 10 children with the Kawasaki-like illness had antibodies to the virus, indicating they had been infected. Positive antibody tests have been reported in sick children elsewhere, too.

It was obvious that there was a link, says Lorenzo DAntiga, a pediatrician at the Papa Giovanni XXIII Hospital who led the study. The new coronavirus can elicit a powerful immune response, which he thinks may explain why shock and a massive immune reaction called a cytokine storm are more common in the COVID-19linked cases than in textbook Kawasaki disease. And a time lag between infection and the Kawasaki-like illness could explain why many of the affected children show no evidence of the virus. The immune systems overreaction may unfold over weeks, though virus could also be hiding somewhere in the body.

Theres clearly some underlying genetic component that puts a small number of children at risk, says Tom Maniatis, founding director of Columbia Universitys Precision Medicine Initiative. New York state is investigating 157 cases, and Maniatis is also CEO of the New York Genome Center, which is pursuing whole-genome sequencing of affected children and their parents, as well as sequencing the virus found in children, with family consent. Finding genes that heighten risk of the illness or of developing a severe case could point to better treatments or help identify children who may take a sudden turn for the worse.

Genetics may also help explain a puzzle: why the illness hasnt been reported in Asian countries, even though Kawasaki disease is far more common in children with Asian ancestry. The virus own genetics may be important; an analysis last month indicatedthe predominant viral variant in New York was brought by travelers from Europe. Its also possible that the Kawasaki-like illness is so rare that it only shows up in COVID-19 hotbeds. The areas that have been hardest hit by coronavirus are the areas reporting this syndrome now, says Alan Schroeder, a critical care physician at Lucile Packard Childrens Hospital at Stanford University, which has seen one potentially affected child, a6-month-old baby, who healed quickly.

Yeung is pursuing ways to flag children with COVID-19 who are at risk of this complication. She co-leads an international consortium thats banking blood from affected children both before and after treatment and screening for various markers, including the cytokine molecules that indicate a revved-up immune system. They are also searching for gene variants known to predict poor outcomes in Kawasaki disease. Theres also core COVID stuff that needs to be measured, Yeung says, such as markers of heart function and levels of D-dimer, a protein fragment in the blood that indicates a tendency toward clotting and that surges in many sick adults.

Another project, called DIAMONDSand originally designed to improve diagnostics of pathogens based on patterns of immune response in children with fevers,is recruiting children across Europe with the Kawasaki-like complication, along with those who have run of the mill COVID-19 symptoms. Scientists will study blood for pathogensnot just SARS-CoV-2and the behavior of immune cells such as T cells and B cells.

We have to do a deep dive into the immunology of those patients, says Elie Haddad, a pediatric immunologist and scientist at the St. Justine University Hospital Center in Montreal who,with Yeung and Susanne Benseler at Alberta Childrens Hospital, is leading Canadian research efforts on the new syndrome. These deep dives may also clarify the immune system chaos seen in many sick adults. Children are cleaner, Haddad points outtheyre less likely to have other health burdens, such as diabetes or high blood pressure, that can make it harder to tease out the virus impact on the immune system.

Its possible, too, that the illness affects adults as well but is harder to tease out from their other symptoms. A global effort studying COVID-19 in adults, called the International Severe Acute Respiratory and Emerging Infection Consortium, will look at adults clinical data and blood samples,Whittaker says, to see, is this a uniquely pediatric problem?

Eager as they are to understand this new face of the pandemic, doctors want to avoid overstating the hazards. We need to identify early and we need to intervene early in treating these children, Yeung says. But she also urges calm. The kids were seeing so far, she stresses, they respond to the treatments were giving.

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Doctors race to understand rare inflammatory condition associated with coronavirus in young people - Science Magazine

Abstract

In mammals, the timing of meiosis entry is regulated by signals from the gonadal environment. All-trans retinoic acid (ATRA) signaling is considered the key pathway that promotes Stra8 (stimulated by retinoic acid 8) expression and, in turn, meiosis entry. This model, however, is debated because it is based on analyzing the effects of exogenous ATRA on ex vivo gonadal cultures, which not accurately reflects the role of endogenous ATRA. Aldh1a1 and Aldh1a2, two retinaldehyde dehydrogenases synthesizing ATRA, are expressed in the mouse ovaries when meiosis initiates. Contrary to the present view, here, we demonstrate that ATRA-responsive cells are scarce in the ovary. Using three distinct gene deletion models for Aldh1a1;Aldh1a2;Aldh1a3, we show that Stra8 expression is independent of ATRA production by ALDH1A proteins and that germ cells progress through meiosis. Together, these data demonstrate that ATRA signaling is dispensable for instructing meiosis initiation in female germ cells.

Germ cells exhibit the unique capacity to generate haploid gametes, eventually giving rise to an embryo after fertilization. In mice, primordial germ cells (PGCs) are specified in the epiblast around 6.25 days post-coitum (dpc) and colonize the gonads at around 10.5 dpc (1). At this stage, the gonads start differentiating as testes in XY embryos, or as ovaries in XX embryos (2). In parallel, germ cells loose pluripotency, becoming either prospermatogonia in testes or oogonia in ovaries, both of which further progress into meiotic divisions (3). However, the timing of the initiation of meiosis is sexually dimorphic (4), starting around 8 days postpartum in the testis versus 13.5 dpc in the ovary.

The nature of the signal(s) instructing oogonia to transition from mitosis to meiosis is still debated. Notably, Stra8 (stimulated by retinoic acid 8) is the only gatekeeper currently described that engages the meiotic program. This is evidenced by the failure of premeiotic DNA replication in female Stra8-deficient gonads (5). As Stra8 was originally identified as an all-trans retinoic acid (ATRA)responsive gene in P19 embryonic carcinoma cells (6), ATRA signaling has been proposed as a primary meiosis-instructing factor. This concept is supported by the up-regulation of meiotic markers including Stra8 in embryonic ovaries cultured ex vivo in the presence of ATRA and ATRA receptor (RAR) agonists, or the down-regulation of Stra8 using pan-RAR antagonists (7, 8). Nevertheless, these findings have been brought into question by experiments that demonstrated Stra8 expression and meiosis initiation in embryonic ovaries lacking two of the three ATRA-synthesizing enzymes (ALDH1A2 and ALDH1A3, encoded by the Aldh1a2 and Aldh1a3 genes) (9). Along the same lines, genetic ablation of Aldh1a1 alone does not impair meiosis, although it reduces Stra8 expression and delays meiosis initiation (10). In both mouse models, the remaining Aldh1a isotype(s) that is (are) remaining may, however, be sufficient to produce ATRA and, as a result, induce meiosis.

To clarify the contribution of endogenous ATRA in vivo, we have generated mice deficient for all three Aldh1a isotypes either in the somatic cells of the embryonic ovary or ubiquitously. Using this approach, we have robustly decreased ATRA signaling during PGC colonization of the developing gonad. Detailed analysis of the mutant phenotypes revealed that ALDH1A1, ALDH1A2, and ALDH1A3 are dispensable for meiotic initiation in oogonia.

It has been shown that two potential sources of ATRA coexist in the female urogenital ridges. First, the mesonephros, a transient organ adjacent to the gonad, exhibits both Aldh1a2 mRNA expression at 10.5 and 12.5 dpc and a strong ATRA responsiveness according to the Tg(RARE-Hspa1b/lacZ)12Jrt transgenic reporter (7, 11). Second, ALDH1A1 and ALDH1A2 expression has been detected in the somatic cells within the developing ovary at 12.5 and 13.5 dpc (10, 12). These findings suggest the existence of both external and endogenous sources of ATRA in the female gonad at the onset of meiosis initiation.

Single-cell RNA sequencing and immune-localization analyses (Fig. 1, A and B) revealed that Aldh1a1 expression became readily detectable in the supporting cells of the ovary at the time of meiosis entry (~13.5 dpc), as previously reported (10, 12). Aldh1a2 exhibited robust expression in the somatic progenitor cells of the gonad and in the mesonephros from 10.5 to 13.5 dpc. At 13.5 dpc, Aldh1a2 was also highly expressed in the supporting cells. Aldh1a3 mRNA was nearly absent in the ovary as evidenced by transcriptomic analysis (Fig. 1A). All these observations indicate that the somatic cells of the ovary, but not the germ cells, are able to synthesize endogenous ATRA as early as 10.5 dpc, i.e., 3 days before meiosis entry.

(A) Violin plots showing the expression of Aldh1a1, Aldh1a2, and Aldh1a3 in the progenitor and supporting cells between 10.5 to 16.5 dpc and postnatal day 6 (E10.5 to E16.5 and P6) determined by single-cell RNA sequencing analysis of Sf1-positive somatic cells from female gonads. Expression values are log-transformed reads per kilobase of transcript per million mapped reads (RPKM); small points represent expression in individual cells, and the white point is the median of expression. Statistical analyses were performed using the Wilcoxon-Mann-Whitney test, and P value was adjusted for false discovery rate. (B) Immunodetection of ALDH1A1 or ALDH1A2 (green) and POU5F1 or TRA98 (germ cells, red) in 10.5, 11.5, and 13.5 dpc ovaries. DAPI (blue), nuclei. Scale bars (white), 50 m. Arrowheads highlight examples of ALDH1A-positive cells. **P <0.01 and ***P <0.001 (adjusted p-values, Wilcoxon-Mann-Whitney test).

Using an ATRA-reporter mouse model carrying the Tg(RARE-Hspa1b/lacZ)12Jrt transgene (11), the mesonephros exhibits a strong response to endogenous ATRA, whereas the ATRA responsiveness is relatively weak in gonads (7, 10), suggesting that only very few cells are responsive to endogenous ATRA in the ovaries. To investigate the nature and fate of these ATRA-responsive cells, we used a novel transgenic reporter mouse line called Tg(RARE-Hspa1b-cre/ERT2), in which RA response elements (RAREs) coupled to the Hspa1b minimal promoter drive expression of the tamoxifen (TAM)inducible CreERT2 recombinase, thus allowing cell lineage tracing of ATRA-responsive cells. In this model, the expression of the membrane-tagged green fluorescent protein (GFP) from the Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo reporter (13) is activated in cells that are ATRA responsive at the time of TAM administration. These cells then become permanently labeled by GFP (fig. S1A). As expected from previous studies (11, 14), the periocular mesenchyme and the developing heart, two tissues that are sensitive to endogenous ATRA, harbored GFP-positive cells ~24 hours after TAM administration (fig. S1B). We then asked whether germ cells in vivo were sensitive to endogenous ATRA. To this aim, TAM was administered to the pregnant females and the gonads from the embryos were collected ~24 hours later for GFP labeling analyses (Fig. 2A). Although a small number of somatic (POU5F1-negative) cells were responsive to endogenous ATRA at 9.5 dpc (evidenced as GFP-positive cells at 10.5 dpc), only a few germ cells were ATRA responsive between 12.5 and 13.5 dpc (because they were GFP positive at 13.5 dpc) (Fig. 2A). Confocal microscopy on whole-mount organs further revealed that the vast majority of germ cells were not ATRA responsive between 13.5 and 14.5 dpc because they were GFP negative at 14.5 dpc (Fig. 2B), although there were a few exceptions (arrowheads in Fig. 2B). Thus, less than 5% of germ cells were ATRA responsive as previously reported (7). We next dissected ovaries at 13.5 dpc and cultured them for 24 hours in the presence of 4-hydroxy-TAM (4-OH-TAM) at either a physiological dose of ATRA (1 nM) (9) or a pharmacological dose of ATRA (100 nM). Dimethyl sulfoxide (DMSO) was used as a negative control (Fig. 2C). Immunostaining analysis using anti-GFP and anti-TRA98 (a germ cell marker) antibodies demonstrated that germ cells from the ex vivo transgenic ovaries lacked GFP-positive germ cells in DMSO-treated samples but were able to respond to a very low concentration of exogenous ATRA (many GFP-positive germ cells in 1 nM ATRAtreated samples). As expected, cells of the mesonephros also responded to ATRA treatment (7). Together, these results indicate that the vast majority of germ cells failed to activate ATRA-responsive genes in vivo, ultimately questioning the requirement of ATRA for meiosis entry.

(A) Immunodetection of ATRA-responsive cells (GFP positive, green) and germ cells (POU5F1- or TRA98-positive cells, red) in 10.5, 11.5, and 13.5 dpc Tg(RARE-Hspa1b-cre/ERT2) ovarian sections after TAM induction at 9.5, 10.5, and 12.5 dpc, respectively. DAPI (blue), nuclei. Scale bars (white), 50 m. (B) Immunodetection of ATRA-responsive cells (GFP, green) and TRA98 (germ cells, red) in 14.5 dpc Tg(RARE-Hspa1b-cre/ERT2) whole ovaries after TAM induction at 13.5 dpc. Scale bars (white), 50 m. White arrowheads, GFP-positive germ cells. (C) Immunodetection of TRA98 (germ cells) (red) and GFP (ATRA-responsive cells) (green) in the presence of either DMSO (control) or 1 or 100 nM ATRA in cultured Tg(RARE-Hspa1b-cre/ERT2) ovaries from 13.5 to 14.5 dpc. Asterisk, GFP-positive cells within the mesonephros.

To functionally test the contribution of ATRA signaling to meiosis entry, we performed conditional deletion of all three ATRA-producing enzymes (Aldh1a1;Aldh1a2;Aldh1a3, hereafter referred to as Aldh1a1-3) using three distinct Cre driver lines (fig. S2, A and B). First, we used the Tg(Nr5a1-cre)2Klp transgenic line (hereafter named Sf1-cre) to induce deletion in SF1-positive somatic cells in the gonads from 11.5 dpc (15). Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis in Sf1-Cre;Aldh1a1-3flox/flox embryos at 13.5 dpc demonstrated the efficient loss of mRNA expression for all three Aldh1a1-3 genes (Fig. 3A). Second, to induce an earlier deletion than the Sf1-Cre, we used the Wt1tm2(cre/ERT2)Wtp strain (16) (hereafter referred to Wt1-CreERT2) that expresses a TAM-inducible CreERT2 recombinase in most, if not all, somatic cells of the gonads at 10.5 dpc (17). TAM administration at 10.5 and 11.5 dpc triggered a strong reduction in Aldh1a1, Aldh1a2, and Aldh1a3 mRNA levels at 13.5 dpc (Fig. 3B). The highly efficient ablation of ALDH1A1 and ALDH1A2 was further confirmed by immunodetection at 13.5 dpc (Fig. 3D). Next, we used the Tg(CAG-cre/ERT2)#Rlb line (18) (hereafter referred to as CAGG-CreER), which ubiquitously expresses a TAM-inducible CreERT2 to delete Aldh1a1-3 in all cell types. In TAM-treated CAGG-CreER;Aldh1a1-3flox/flox ovaries, Aldh1a1, Aldh1a2, and Aldh1a3 mRNA levels were significantly reduced in the gonads, as previously reported (19). This was accompanied by a significant decrease of ALDH1A1 and ALDH1A2 protein levels at 13.5 dpc, resulting in severe heart malformations (Fig. 3E and fig. S2C). To summarize, all three mouse models demonstrated efficient elimination of Aldh1a1, Aldh1a2, and Aldh1a3 expression in the embryonic ovaries.

(A) RT-qPCR analysis of Aldh1a1, Aldh1a2, and Aldh1a3 expression in 13.5 dpc control (orange) and Sf1-Cre;Aldh1a1-3flox/flox (blue) gonads. Students t test, unpaired. Bars represent mean + SEM; n = 10 individual gonads. ***P < 0.001. (B) Top: Protocol of induction of Aldh1a1-3 deletion (10.5 dpc onward). Bottom: RT-qPCR analysis of Aldh1a1, Aldh1a2, and Aldh1a3 expression in 13.5 dpc control (orange) and Wt1-CreERT2;Aldh1a1-3flox/flox (blue) gonads. Students t test, unpaired. Bars represent mean + SEM; n = 10 individual gonads. ***P < 0.001. (C) Top: Protocol of induction of Aldh1a1-3 deletion (10.5 dpc onward). Bottom: RT-qPCR analysis of Aldh1a1, Aldh1a2, and Aldh1a3 expression in 13.5 dpc control (orange) and CAGG-CreER;Aldh1a1-3 flox/flox (blue) gonads. However, the deletion was less efficient than using the Wt1-CreERT2 or Sf1-Cre transgenes. Students t test, unpaired. Bars represent mean + SEM; n = 15 individual gonads. ***P < 0.001. (D) Immunodetection of ALDH1A1 or ALDH1A2 (green) and POU5F1 (germ cells, red) in 13.5 dpc control and Wt1-CreERT2;Aldh1a1-3flox/flox ovaries. DAPI (blue), nuclei. Scale bars (white), 50 m. (E) Immunodetection of ALDH1A1 or ALDH1A2 (green) and CDH1 (germ cells, red) in 13.5 dpc control and CGAG-CreERT2;Aldh1a1-3 flox/flox ovaries. DAPI (blue), nuclei. Scale bars (white), 50 m.

To assess the impact of Aldh1a1-3 deletion on ATRA synthesis, we performed metabolomic investigations and evaluated endogenous production of ATRA in mesonephroi, control testes, control ovaries, or Wt1-CreERT2;Aldh1a1-3flox/flox ovaries from 13.5 dpc embryos (fig. S3A). RA can be isomerized in ATRA, the most abundant form, in 9-cis-RA (9cRA), which is almost undetectable in vivo, and in 13-cis-RA (13cRA), which is mostly present in the serum (20). RA isomers have different affinities for nuclear receptors and therefore exert different biological activities: ATRA has the highest affinity for RA receptors (RAR) and 9cRA can bind RAR/RXR. In contrast, 13cRA has a 100-fold lower affinity to RARs than the two others (21). Using single-ion mass spectrometry, we were able to detect all three RA isomers in vivo, even 9cRA isomer, which shows the high sensitivity of this method, and to quantify the relative abundance of ATRA isomer (fig. S3A). The ATRA level was abundant in mesonephroi as previously described (7) but was strongly decreased in Wt1-CreERT2;Aldh1a1-3flox/flox ovaries compared to control ovaries. In addition, this level was similar to the level detected in testes (fig. S3, B and C).

To determine whether this level of ATRA was sufficient to trigger a biological activity in the mutant ovaries, we designed an experimental ATRA-reporter assay by transfecting Chinese hamster ovary (CHO) cells with a plasmid containing the ATRA-responsive hsp68 mouse promoter (11) controlling the expression of the acGFP1 gene encoding the Aequorea coerulescens GFP. We cultured the transfected CHO cells in the absence or presence of increasing ATRA concentrations, or in the presence of cellular suspensions from either mesonephroi, control, or Wt1-CreERT2;Aldh1a1-3flox/flox ovaries dissected from 13.5 dpc embryos (Fig. 4A). Although GFP expression (quantified by Western blot) was stimulated by commercial ATRA from 1 nM onward, mesonephroi, or control ovaries, the GFP level of expression induced by mutant ovaries barely reached the residual level of GFP measured in cultures with medium depleted in RA, confirming that the genetic deletion of Aldh1a1-3 was efficient enough to inhibit ATRA synthesis (Fig. 4B).

(A) CHO cells transfected with the ATRA-reporter construct and incubated with either DMSO (vehicle), increasing concentrations of ATRA from 1 to 100 nM, cellular suspensions of mesonephroi, Aldh1a1-3 flox/flox, or Wt1-CreERT2;Aldh1a1-3flox/flox ovaries dissected from 13.5 dpc embryos (top, brightfield picture; bottom, GFP detection). (B) Immunodetection by Western blot of GFP and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) in the same CHO cells transfected (+) or not () with the ATRA-reporter construct (RARE-acGFP1 plasmid) after protein extraction (upper), and corresponding quantification of GFP band intensity after normalization with GAPDH expression (lower). (C) RT-qPCR analysis of RAR, Cyp26a1, Crabp1, and Crabp2 expression in 13.5 dpc control (orange), Wt1-CreERT2;Aldh1a1-3flox/flox (blue) gonads, or whole 10.5 dpc embryos (positive control, gray). Students t test, unpaired. Bars represent mean + SEM; n = 10 individual gonads. ***P < 0.001.

We next analyzed the expression of RAR, Cyp26a1, and Crabp1-2, four ATRA-target genes used to monitor the inhibition of RA signaling (9) in control and Wt1-CreERT2;Aldh1a1-3flox/flox ovaries at 13.5 dpc. Although RAR, Crabp1, and Crabp2 mRNA levels were significantly down-regulated in the Wt1-CreERT2;Aldh1a1-3flox/flox ovaries compared to the controls, we did not detect any Cyp26a1 expression, neither in the control nor in the mutant ovaries (Fig. 4C), demonstrating that RA signaling was impaired in Wt1-CreERT2;Aldh1a1-3flox/flox ovaries. Together, these results indicate that the very low level of ATRA remaining in Wt1-CreERT2;Aldh1a1-3flox/flox ovaries was not sufficient to promote the expression of a RARE reporter and of universal target genes.

Because ATRA signaling has been reported to regulate germ cell proliferation in the developing ovary (22), we next examined the proliferation of POU5F1- or DDX4-positive germ cells at 11.5 and 12.5 dpc in control and Wt1-CreERT2;Aldh1a1-3flox/flox ovaries treated by TAM at 9.5 and 10.5 dpc. A 3-hour pulse of 5-bromo-2-deoxyuridine (BrdU) incorporation permanently labeled the cells transitioning through the S phase of the cell cycle. Quantification of the number of BrdU-positive germ cells indicated that despite the significant loss of Aldh1a1, Aldh1a2, and Aldh1a3 expression upon TAM treatment (Fig. 5A), proliferation was not affected in Wt1-CreERT2;Aldh1a1-3flox/flox gonads (Fig. 5B). Moreover, the pluripotency markers Pou5f1 (also known as Oct3/4), Sox2, and Dazl, driving oogonia differentiation (23), were expressed at similar levels in control and Aldh1a1-3deficient gonads at 11.5 and 12.5 dpc (fig. S4). Together, these results indicate that the genetic ablation of ALDH1A1, ALDH1A2, and ALDH1A3 does not hinder the proliferation or differentiation of PGCs.

(A) Protocol of induction of Aldh1a1-3 deletion (9.5 dpc onward). RT-qPCR analysis of Aldh1a1, Aldh1a2, and Aldh1a3 expression in 11.5 dpc control (orange) and Wt1-CreERT2;Aldh1a1-3flox/flox (blue) ovaries. Students t test, unpaired. Bars represent mean + SEM; n = 10 individual gonads. ***P < 0.001. (B) Immunodetection of BrdU (proliferating cells, green), POU5F1 (PGCs, red), and GATA4 (gonadal somatic cells, cyan) at 11.5 dpc in control (left) and Wt1-CreERT2;Aldh1a1-3flox/flox (right) ovaries. Histograms: Percentage of BrdU-positive (proliferating) versus POU5F1-positive (total) germ cells in control (orange) and Wt1-CreERT2;Aldh1a1-3flox/flox (mutant, blue) ovaries at 11.5 dpc. Students t test, unpaired. Bars represent mean + SEM; n = 10 sections of each genotype (four to seven ovaries per genotype). ns, not significant. Bottom: Immunodetection of BrdU (proliferating cells, green) and DDX4 (germ cells, red) at 12.5 dpc in control (left) and Wt1-CreERT2;Aldh1a1-3flox/flox (right) ovaries. Histograms: Percentage of BrdU-positive (proliferating) versus DDX4-positive (total) germ cells in control (orange) and Wt1-CreERT2;Aldh1a1-3flox/flox (mutant, blue) ovaries at 12.5 dpc. Students t test, unpaired. Bars represent mean + SEM; n = 10 sections of each genotype (four to seven ovaries per genotype). Arrowheads highlight examples of BrdU-positive germ cells.

We next assessed whether germ cells initiated meiosis after genetic deletion of Aldh1a1-3 using the different mouse models described above. In Sf1-Cre;Aldh1a1-3flox/flox embryos, Stra8 expression was reduced by ~20% at 13.5 dpc (fig. S5A). Stra8 expression was also reduced at 13.5 dpc in ovaries of Wt1-CreERT2;Aldh1a1-3flox/flox embryos treated by TAM at 9.5 and 10.5 dpc (fig. S5B). Nevertheless, under these conditions, TAM treatment induced frequent embryonic lethality. Accordingly, TAM was administrated at 10.5 and 11.5 dpc in the following experiments, leading to an almost identical decrease of Aldh1a1 and Aldh1a2 mRNA expression (fig. S6). Upon this TAM treatment, germ cells in Wt1-CreERT2;Aldh1a1-3flox/flox ovaries expressed Stra8 at 13.5 dpc, although its mRNA levels were reduced by ~25% when compared to controls (Fig. 6, A and C). At 14.5 dpc, this deficit was partly compensated in the Wt1-CreERT2;Aldh1a1-3flox/flox ovaries, suggesting a delay in the onset of Stra8 expression in the absence of ALDH1A isotypes (Fig. 6B). Notably, the reduction in Stra8 expression did not impair meiosis entry, as evidenced by the normal levels of Rec8 mRNA, a gene that encodes a component of the cohesin complex accumulating during the meiotic S phase (Fig. 6B), and of Spo11 mRNA, which is expressed during the leptotene stage of meiotic prophase I (Fig. 6A). Together, these results demonstrate that genetic deletion of the three Aldh1a1-3 genes does not impair meiosis entry.

(A) RT-qPCR analysis of Stra8, Rec8, and Spo11 expression in 13.5 dpc control (orange) and Wt1-CreERT2;Aldh1a1-3flox/flox (blue) ovaries. Students t test, unpaired. Bars represent mean + SEM; n = 10 individual ovaries. **P < 0.01. (B) RT-qPCR analysis of Pou5f1, Dazl, Stra8, and Rec8 expression in 14.5 dpc control (orange) and Wt1-CreERT2;Aldh1a1-3flox/flox (blue) ovaries. Students t test, unpaired. Bars represent mean + SEM; n = 10 individual gonads. *P < 0.05. (C) In situ hybridization using Stra8 riboprobe at 13.5 dpc in control (left) and Wt1-CreERT2;Aldh1a1-3flox/flox (right) ovaries. Scale bars (white), 50 m. (D) Immunodetection of DAZL (red) and SYCP3 (green) in 16.5 dpc control and Wt1-CreERT2;Aldh1a1-3flox/flox ovaries. Bottom: Immunodetection of phosphohistone H2AX (red) and DDX4 (green) in 16.5 dpc control and mutant ovaries. DAPI (blue), nuclei. Scale bars (white), 50 m.

We next examined whether meiosis further progressed despite Aldh1a1-3 ablation by looking at specific chromosomal features of meiosis. During meiotic progression, homologous chromosomes pair and the synaptonemal complex promotes chromosome recombination. Immunodetection of the synaptonemal complex protein 3 (SYCP3), which appears in leptotene stage and becomes enriched in the zygotene stage, revealed similar thread-like synaptonemal complex structures in TAM-treated control and Wt1-CreERT2;Aldh1a1-3flox/flox ovaries (Fig. 6D), further demonstrating that germ cells entered meiosis in the absence of all ALDH1A isotypes. Quantification of the germ cells positive for phospho-H2AX, which is associated with DNA double-strand breaks occurring during the leptotene stage, confirmed these observations (Fig. 6D and fig. S7). Hence, these results demonstrate that female germ cells progress to meiotic prophase even when Aldh1a1-3 are deleted from the ovary from 10.5 dpc onward.

In TAM-treated CAGG-CreER;Aldh1a1-3flox/flox ovaries, the mRNA levels of meiotic markers such as Mei1, Dmc1, Rec8, Syce1, and Spo11 were not significantly changed as evidenced by RT-qPCR and in situ hybridization experiments (fig. S8, A and B). Accordingly, phospho-H2AX expression was comparable between TAM-treated control and CAGG-CreER;Aldh1a1-3flox/flox ovaries at 16.5 dpc (fig. S8C). Together, these results indicate that genetic ablation of Aldh1a1-3 does not prevent meiosis entry in the developing mouse ovary.

Evidence that PGCs do not enter meiosis because of an intrinsic autonomous property (i.e., cell division timing) but are rather instructed by a signaling molecule produced by somatic ovarian cells led to a search for a meiosis-inducing substance (MIS) (24). Although ATRA has been suggested to be one such MIS, its role in this biological process has been questioned, giving rise to conflicting reports (79). In all three distinct models of Aldh1a1-3 genetic deletion we have used, we observed only a minor decrease in Stra8 expression, and this reduction was not robust enough to prevent meiosis initiation and germ cells from progressing into meiotic prophase I. Thus, we conclude that ATRA signaling does not initiate but contributes to meiosis by making Stra8 expression on time. Accordingly, ectopic expression of Cyp26a1, which has the most efficient catalytic activity on ATRA degradation, does not affect meiosis entry or Stra8 expression, although Stra8 mRNA levels were reduced by ~30%, i.e., in the same range than the mRNA reduction observed in the present study (25). This indicates that ATRA does regulate Stra8 transcription maintenance rather than Stra8 initiation of transcription. In agreement with our findings, the study from Vernet et al. (this issue) describes a similar delay in expression of Stra8 and a normal progression into meiosis in fetal ovaries of mice lacking the RARs RAR, RAR, and RAR. Because the fetal lethality in our different mice models prevented us to study the postnatal ovary, the fate of the ATRA-responsive female germ cells in vivo requires further investigations.

When identified, Stra8 was not classified in the class of immediate and early, ATRA-responsive gene, as indicated by the kinetics of its mRNA accumulation in P19 pluripotent carcinoma cells treated with ATRA (6). It was shown that ATRA regulates the phosphorylation status of the STRA8 protein in P19 cells (6), indicating that ATRA might also control STRA8 posttranslational modifications. The functional relevance of ATRA-induced phosphorylation in STRA8 activity or stability has not been characterized. Nevertheless, our results indicate that Aldh1a1-3 are not required for Stra8 expression in germ cells, suggesting that either ATRA does not phosphorylate STRA8 in vivo or STRA8 phosphorylation has no impact on its activity in germ cells. Together, our results, those from Vernet et al. and Bellutti et al. (25), indicate that ATRA signaling is dispensable for meiosis entry.

In PGC-like cells (PGCLCs) derived from embryonic stem cells, Stra8 expression is induced without ATRA treatment (26), further highlighting that ATRA is not instrumental for meiosis initiation in this system and that other signals are responsible for initiation of Stra8 expression. In vitro generation of female PGCLCs expressing Stra8, SYCP3, and other meiotic markers requires bone morphogenetic proteins (BMPs), which activate transforming growth factor (TGF)/SMAD signaling. In these experiments, ATRA increased the number of meiotic germ cells, indicating that ATRA signaling served to enhance a preexisting situation (26).

Meiosis initiation is timed by epigenetic factors such as polycomb repressive complex PRC1 that promotes structural modifications of chromatin and consequently times the expression of Stra8 (27). Recent data show that deficiency in vitamin C during gestation induces incomplete DNA demethylation of key germline genes and thus delays meiosis initiation in the embryos (28), indicating that molecules from the maternal nutrition participate in regulating meiotic gene expression in the germ cells of the progeny. In addition, in the absence of Rspo1, an activator of WNT/-catenin signaling in the female fetal gonad, a proportion of PGCs neither expressed Stra8 nor entered meiosis (29), suggesting a control of Stra8 expression by WNT/-catenin. The Msx genes, which encode homeodomain transcription factors, are direct targets of WNT/-catenin signaling in murine embryonic stem cells (30), and in mutant embryos lacking both Msx1 and Msx2, germ cells failed to initiate meiosis (31). In gonadal cultures, BMP4 stimulates the expression of Msx1 and Msx2 that, in turn, directly regulate Stra8 expression (29), suggesting that TGF signaling is also involved in Stra8 regulation. However, in mouse germ cells, the central transducer Smad4 is dispensable for Stra8 expression, although it is required to up-regulate other key genes involved in meiosis (30). Last, the DMRT1 transcription factor also contributes to the switch from mitosis to meiosis by directly regulating Stra8 expression in female germ cells (32). Together, these studies indicate that convergent pathways other than ATRA signaling collaborate to regulate Stra8 expression and the transition from mitosis to meiosis cycle.

Early studies based on the observation that female germ cells cultured together with fetal testes were prevented from initiating meiosis (33, 34) led to the concept of a secreted masculinizing meiosis preventing substance (MPS) in the male gonad (35). The P450 enzyme CYP26B1, expressed in the supporting cells of the fetal testis but not the ovary, has been proposed to be MPS. Analysis of Cyp26b1-null mutant mice demonstrated that CYP26B1 activity prevented germ cells from entering into meiosis in male mice (36). Whereas CYP26B1 is able to degrade ATRA (37), the data from Kumar et al. and Bellutti et al. (25) suggest that CYP26B1 metabolizes a substrate other than ATRA to prevent meiosis initiation (9). So far, knowledge about the metabolic ligands of CYP26B1 is poor, and the nature and the molecular identity of CYP26B1 substrate(s) in the fetal testis remain unknown.

Identifying the molecules controlling the fundamental decision of germ cell to enter meiosis and defining whether they have MPS or MIS functions represents a major challenge for the reproductive medicine community in the upcoming years. The main clinical consequences of defects in germ cell development are infertility and increased susceptibility to germ cell tumors. Therefore, understanding how germ cells change their gene expression profiles in response to somatic signals will provide knowledge on the etiology of human genetic diseases.

The aim of this study was to investigate the role of endogenous ATRA signaling by using an in vivo genetic approach of deletion of Aldh1a1, Aldh1a2, and Aldh1a3 genes (i.e., mice lacking all sources of ATRA in vivo) to challenge the admitted concept of ATRA being the MIS. The number of samples was determined on the basis of experimental approach, availability, and feasibility required to obtain definitive results. No data were excluded from the analyses. The numbers of replicates are specified in Materials and Methods. The researchers were not blinded during data collection or analysis.

The experiments described here were carried out in compliance with the relevant institutional and European animal welfare laws, guidelines, and policies. All the experiments were approved by the French Ministre de lEducation Nationale, de lEnseignement Suprieur et de la Recherche (APAFIS # 3771-2016012110545580v13). All mice were kept on a 129/Sv-C57BL/6J mixed background. Mouse lines were obtained from the Jackson Laboratory. The Wt1tm2(cre/ERT2)Wtp, Tg(Nr5a1-cre)2Klp, Tg(CAG-cre/ERT2)#Rlb, Aldh1a1flox/flox, Aldh1a2flox/flox, Aldh1a3flox/flox, and Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo mice were described previously and genotyped as reported (13, 15, 16, 38, 39, 40). Genotyping was performed using DNA extracted from tail tips or ear biopsies of mice. To activate the CreERT recombinase in embryos, TAM (catalog no. T5648, Sigma-Aldrich) was directly diluted in corn oil to a concentration of 40 mg/ml, and two TAM treatments (200 mg/kg body weight) were administrated to pregnant females by force-feeding at 9.5 and 10.5 dpc or 10.5 and 11.5 dpc. This resulted in embryos in which Aldh1a1-3 were deleted upon TAM induction when they were carrying the creERT transgene in contrast with their control littermates. For proliferation assays, BrdU (catalog no. B5002, Sigma-Aldrich) was diluted to a concentration of 10 mg/ml in sterile H2O and administrated to the pregnant females at a final concentration of 10 g/ml by intraperitoneal injection, and pregnant females and their embryos were sacrificed after 3 hours.

Single-cell RNA sequencing was performed as described in (41). Briefly, somatic cells of developing mouse female gonads were purified by fluorescence-activated cell sorting (FACS) using the Tg(Nr5a1-GFP) at six stages of development (10.5, 11.5, 12.5, 13.5, and 16.5 dpc and postnatal day 6). Single-cell isolation, reverse transcription, and complementary DNA (cDNA) amplification were performed using the Fluidigm C1 Auto Prep system, and single-cell sequencing library was prepared with Illumina Nextera XT following Fluidigm protocol. Library was multiplexed and sequenced on an Illumina HiSeq2000 platform with 100base pair (bp) paired-end reads at an average depth of 10 million reads per single cell. Obtained reads were mapped on the mouse reference genome (GRCm38.p3), and gene expression was quantified in RPKMs (reads per kilobase of exon per million reads mapped). Cells were clustered using principal components analysis and hierarchical clustering, and cell types were identified according to the expression level of marker genes and gene ontology enrichment tests. Significance of the difference in expression level between cell types was assessed in R version 3.6.0 using Wilcoxon-Mann-Whitney tests, and P values were adjusted for false discovery rate using Bonferroni.

Individual gonads without mesonephroi were dissected in phosphate-buffered saline (PBS) from 11.5, 12.5, 13.5, and 14.5 dpc embryos. RNA was extracted using the Qiagen RNeasy Kit and reverse-transcribed using the RNA RT-PCR Kit (Stratagene). Primers and probes were designed by the Roche Assay Design Center (https://www.rocheappliedscience.com/sis/rtpcr/upl/adc.jsp). All real-time PCR assays were carried out using the LightCycler FastStart DNA Master Kit (Roche) according to the manufacturers instructions. qPCR was performed on cDNA from one gonad and compared to a standard curve. They were repeated at least twice. Relative expression levels of each sample were quantified in the same run and normalized by measuring Sdha expression (which represents the total gonadal cells). For Aldh1a1, Aldh1a2, and Aldh1a3 real-time PCR, the following specific primers were used: 5-actttcccaccattgagtgc-3 and 5-caccatggatgcttcagaga-3 (Aldh1a1), 5-catggtatcctccgcaatg-3 and 5-gcgcatttaaggcattgtaac-3 (Aldh1a2), and 5-tctgggaatggcagagaact-3 and 5-ttgatggtgacggttttcac-3 (Aldh1a3). For each sample, relative expression levels were quantified and normalized. For each genotype (n = 10), the mean of these 10 absolute expression levels (i.e., normalized) was calculated and then divided by the mean of the 10 absolute expression levels of the control samples considered as the reference (=1 when divided by itself), leading to the fold of change.

For each genotype, the mean of the normalized expression levels was calculated, and graphs show mean fold change + SEM. All the data were analyzed by Students t test using Microsoft Excel. Asterisks highlight the pertinent comparisons and indicate levels of significance: *P < 0.05, **P < 0.01, and ***P < 0.001. Data are shown as mean + SEM.

For metabolomic analysis, 12 mesonephroi or 12 gonads of each genotype (Aldh1a1-3flox/flox ovaries, Aldh1a1-3flox/flox testes, Wt1-CreERT2;Aldh1a1-3flox/flox ovaries) from 13.5 dpc littermate embryos were dissected and subjected to methanol-chloroform extraction. The methanol phase was collected for mass spectrometry, whereas the interphase was used for quantification of protein concentration. Single ion monitoring analysis of RA was performed using a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). Compounds were loaded onto a Phenomenex Synergi 4 m Hydro-RP 80A 250 2 mm. RA isomers were separated with a 25-min gradient at a flow rate of 0.350 ml/min (mobile phase A water and 0.1% formic acid, mobile phase B acetonitrile and 0.1% formic acid gradient, 30% A for 5 min and 90% B in 15-min return initial condition for 5 min). The column was then thoroughly cleaned with 10 blank runs using a 2-l injection of 30% MeCN, 30% isopropanol, and 0.1% formic acid after each run. The mass spectrometry method was done in positive electrospray ionization mode, which increases the sensitivity for RA isomers. We used a targeted SIM (Selected Ion Monitoring) scan on the M+H: 301.216. This led to an improved signal-to-noise ratio and to lower detection limits. The method consisted of full scans and targeted SIM scan. Full scans were acquired with AGC (Automatic Gain Control) target value of 1 106, resolution of 70,000 full width at half maximum (FWHM) at 200 mass/charge ratio (m/z), and maximum ion injection time of 100 ms. The target was monitored with a 4-min window, AGC target value of 1 105, resolution of 280,000 FWHM at 200 m/z, and maximum ion injection time of 500 ms.

RA levels (all isomers) were quantified by normalizing the peak area given by the mass spectrometer by the protein concentration of each sample. ATRA-specific peak area was measured using ImageJ software and normalized with the protein concentration.

CHO cells were cultured in Dulbeccos minimum essential medium (DMEM):F12 medium (1:1) (Gibco) supplemented with recombinant human epidermal growth factor (EGF) (10 ng/ml; catalog no. PHG0314, Gibco), ITS (insulin-transferrin-selenium; 100; catalog no. 41400045, Gibco), and 100 nonessential amino acids (catalog no. 1140068, Gibco) in the absence of serum. Cells (15,000 per well) were seeded in a 24-well plate 24 hours before transfection. Then, CHO cells were transfected with 800 ng of plasmid described hereafter, using Lipofectamine 2000 reagent (catalog no. 11668019, Invitrogen) according to the manufacturers procedure. The ATRA-reporter construct was designed as follows: A DNA fragment encompassing the RA-responsive hsp68 mouse promoter as previously published (10), the rabbit -globin intron, the coding sequence of the acGFP1 gene (Aequorea coerulescens GFP), two STOP codons, and the SV40 late polyadenylation signal was synthesized by Sigma-Aldrich and cloned by the manufacturer in the kanamycin-resistant plasmid pUC57. Two copies in tandem of the 5HS4 insulator were then added by cloning in appropriate restriction sites at the 3 end of the polyadenylation signal. The sequence of the whole construct is available upon request. Twenty-four hours after transfection, CHO cells were incubated with either DMSO (vehicle), commercial ATRA (catalog no. R2625, Sigma-Aldrich) from 1 to 100 nM in DMSO, or cellular suspensions from mesonephroi, Aldh1a1-3flox/flox, or Wt1-CreERT2;Aldh1a1-3flox/flox ovaries from 13.5 dpc littermate embryos freshly dissected (n = 6 mesonephroi or gonads per experiment, experiment performed in two replicates). Twenty-four hours later, GFP endogenous signal was visualized with an Axio Imager Z1 microscope (Zeiss) coupled with an AxioCam MRm camera (Zeiss) and images were processed with AxioVision LE and ImageJ.

Cells were solubilized in 50-ml lysis buffer containing 50 mM tris-HCl (pH 7.4), 200 mM NaCl, 1 mM EDTA, 0.2% NP-40, protease inhibitor (cOmplete; catalog no. 4693116001, Sigma-Aldrich), and phosphatase inhibitor cocktails (PhosSTOP; catalog no. 4906845001, Sigma-Aldrich). Proteins (25 mg per lane) were resolved on SDS polyacrylamide gel electrophoresis and transferred onto Immobilon-P membrane (Millipore). The membrane was saturated with PBS supplemented with 5% milk and 0.1% Tween 20 and incubated with the following antibodies: GFP (1:1000; catalog no. ab6673, Abcam) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000; sc32233, Santa Cruz Biotechnology). Western blot chemiluminescence detection was performed using a Fusion FX7 Spectra imager (Vilber). Band intensity was quantified and normalized using ImageJ software.

Samples were dissected and fixed overnight with 4% (w/v) paraformaldehyde and then processed for paraffin embedding. Microtome sections of 7-m thickness were processed for in situ hybridization. Stra8 digoxigenin-labeled riboprobe was synthesized, and in situ hybridization analyses were performed as described in (29). Imaging was performed on an MZ9.5 microscope (Leica) coupled with a DHC490 camera (Leica) and Leica application suite V3.3.0 software and processed with Adobe Photoshop. For each genotype, n = 3 to 5 embryos.

Samples were fixed overnight with 4% (w/v) paraformaldehyde and then processed either for paraffin embedding or directly for whole-mount immunostaining. Microtome sections of 5-m thickness were processed for immunostaining. Immunofluorescence analyses were performed as described (29). The following dilutions of primary antibodies were used: ALDH1A1 (1:50; catalog no. 52492, Abcam), ALDH1A2 (1:500; catalog no. HPA010022, Sigma-Aldrich), CDH1 (1:100; catalog no. 610182, BD Transduction Laboratories), DAZL (1:200; catalog no. GTX89448, GeneTex), DDX4 (1:200; catalog no. 13840, Abcam), GATA4 (1:200; catalog no. 1237, Santa Cruz Biotechnology), GFP (1:750; catalog no. TP401, Torrey Pines Bio Labs), POU5F1 (1:250; catalog no. 611202, BD Transduction Laboratories), phospho-H2AX (1:300; catalog no. 16193, Millipore), SOX2 (1:200; catalog no. 97959, Abcam), SYCP3 (1:200; catalog no. 15093, Abcam), TRA98 (1:150; catalog no. 82527, Abcam), and FUT4 (SSEA1) (1:200; catalog no. 21702, Santa Cruz Biotechnology). Slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI) diluted in the mounting medium at 10 g/ml (Vectashield, Vector Laboratories) to detect nuclei. Imaging was performed with a motorized Axio Imager Z1 microscope (Zeiss) coupled with an AxioCam MRm camera (Zeiss), and images were processed with AxioVision LE and ImageJ. ImageJ software was used for quantification of proliferating germ cells versus total germ cells. Whole-organ imaging was performed on an LSM 780 NLO inverted Axio Observer Z1 confocal microscope (Carl Zeiss Microscopy GmbH, Jena, Germany) using a Plan Apo 10 dry NA (numerical aperture) 0.45 objective. In monophoton mode, images were acquired using an argon laser (488 nm) and DPSS (green and yellow diode-pumped solid state) (561 nm). The microscope z-drive was used for z acquisitions, and an automated xy stage was used for multiposition recording acquisitions (Mrzhuser, Wetzlar, Germany). For each genotype, n = 3 to 5 embryos.

Paraffin sections from each genotype were processed for immunohistological experiments with POU5F1, DDX4, or GATA4 antibody. Then, proliferation analysis was performed on the same sections by BrdU labeling, and detection was performed using an appropriate kit (catalog no. 11 296 736 001, Roche). Total germ cells and proliferating cells were quantified on the entire section using ImageJ software. For each picture, the number of BrdU-positive germ cells (proliferating) and the number of either OCT4- or DDX4-positive cells (total) were counted. Then, the percentage of BrdU-positive versus OCT4- or DDX4-positive germ cells was determined. For each genotype (n = 4 to 7; 15 pictures per genotype), the mean and mean + SEM of these percentages were calculated and reported on a graph after statistical analysis (for details, see the Statistical analysis section).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

Acknowledgments: We thank L. Turchi, F. Massa, S. Lachambre, and S. Rekima for their help. Funding: This work was supported by grants from the Agence Nationale de la Recherche (ANR-13-BSV2-0017-02 ARGONADS, ANR-11-LABX-0028-01, and ANR-18-CE14-0012 SexSpecs). M.L.R. was supported by a fellowship from La Ligue Nationale Contre le Cancer. A.S. was supported by a grant from La Ligue Nationale Contre le Cancer (Equipe Labellise Ligue Nationale Contre le Cancer). The microscopy was done in the Prism facility, Plateforme Prism, and the histological experiments were done at the Experimental Histopathology Platform, IBV-CNRS UMR 7277-INSERM U1091-UNS. Author contributions: A.-A.C., M.-C.C., and N.B.G. designed the study, analyzed the data, and wrote the paper. A.-A.C., M.L.R., I.S., G.J., J.-M.G., and F.D.S. performed the experiments and analyzed the data. E.P., S.N., and A.S. discussed the results and commented on the manuscript. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.

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Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary - Science Advances

Global Precision Medicine Marketto grow with a substantial CAGR in the forecast period of 2019-2026. Growing prevalence of cancer worldwide and accelerating demand of novel therapies to prevent of cancer related disorders are the key factors for lucrative growth of market

Key Market Players:

Few of the major competitors currently working in the global precision medicine market are Neon Therapeutics, Moderna, Inc, Merck & Co., Inc, Bayer AG, PERSONALIS INC, GENOCEA BIOSCIENCES, INC., F. Hoffmann-La Roche Ltd, CureVac AG, CELLDEX THERAPEUTICS, BIONTECH SE, Advaxis, Inc, GlaxoSmithKline plc, Bioven International Sdn Bhd, Agenus Inc., Immatics Biotechnologies GmbH, Immunovative Therapies, Bristol-Myers Squibb Company, Gritstone Oncology, NantKwest, Inc among others.

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Global Precision Medicine MarketBy Application (Diagnostics, Therapeutics and Others), Technologies (Pharmacogenomics, Point-of-Care Testing, Stem Cell Therapy, Pharmacoproteomics and Others), Indication (Oncology, Central Nervous System (CNS) Disorders, Immunology Disorders, Respiratory Disorders, Others), Drugs (Alectinib, Osimertinib, Mepolizumab,Aripiprazole lauroxil and Others), Route of Administration (Oral,Injectable), End- Users (Hospitals, Homecare, Specialty Clinics, Others), Geography (North America, South America, Europe, Asia-Pacific, Middle East and Africa) Industry Trends and Forecast to 2026

Competitive Analysis:

The precision medicine market is highly fragmented and is based on new product launches and clinical results of products. Hence the major players have used various strategies such as new product launches, clinical trials, market initiatives, high expense on research and development, agreements, joint ventures, partnerships, acquisitions, and others to increase their footprints in this market. The report includes market shares of mass spectrometry market for global, Europe, North America, Asia Pacific and South America.

Market Drivers

Market Restraints

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Market Definition:

Precision medicines is also known as personalized medicines is an innovative approach to the patient care for disease treatment, diagnosis and prevention base on the persons individual genes. It allows doctors or physicians to select treatment option based on the patients genetic understanding of their disease.

According to the data published in PerMedCoalition, it was estimated that the USFDA has approved 25 novels personalized medicines in the year of 2018. These growing approvals annually by the regulatory authorities and rise in oncology and CNS disorders worldwide are the key factors for market growth.

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Key Developments in the Market:

Competitive Analysis:

Global precision medicine market is highly fragmented and the major players have used various strategies such as new product launches, expansions, agreements, joint ventures, partnerships, acquisitions, and others to increase their footprints in this market. The report includes market shares of global precision medicine market for Global, Europe, North America, Asia-Pacific, South America and Middle East & Africa.

Market Segmentation:

By technology:-big data analytics, bioinformatics, gene sequencing, drug discovery, companion diagnostics, and others.

By application:- oncology, hematology, infectious diseases, cardiology, neurology, endocrinology, pulmonary diseases, ophthalmology, metabolic diseases, pharmagenomics, and others.

On the basis of end-users:- pharmaceuticals, biotechnology, diagnostic companies, laboratories, and healthcare it specialist.

On the basis of geography:- North America & South America, Europe, Asia-Pacific, and Middle East & Africa. U.S., Canada, Germany, France, U.K., Netherlands, Switzerland, Turkey, Russia, China, India, South Korea, Japan, Australia, Singapore, Saudi Arabia, South Africa, and Brazil among others.

In 2017, North America is expected to dominate the market.

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COVID-19 UPDATE: Precision Medicine Market 2020: Industry Analysis and Detailed Profiles of Top Industry Players are Neon Therapeutics, Moderna, Inc,...

In 2013, a mysterious new flu virus hit the world. It was the H7N9 strain of bird flu, and by the time it infected 120 and killed 23, researchers still didnt know how it was transmitted or how to stop it. As the virus arrived in the dense Chinese province of Hunan, health officials began to fear the worst.

In the U.S., they called Dan Gibson. Gibson was Vice President of DNA Technology atSynthetic Genomics, the research institute founded in 2005 by genomics pioneers Craig Venter, Ph.D., and Nobel Laureate Hamilton Smith, M.D., shortly after the completion of the Human Genome Project. Among its research milestones, the institute created the first synthetic cell, designed in a computer and DNA-printed completely from scratch.

Gibson was given a download link to the DNA sequence of the bird flu virus and told to do one thing: design a vaccine.

Gibson is the namesake of the Gibson assembly method, the worlds leading branded method of joining DNA fragments together that he pioneered just four years before. The method lets you join DNA fragments together in a single step, and it proved to have a huge multiplying effect on the entire field ofsynthetic biology. So who better to ask for a DNA solution to the bird flu?

Dan Gibson is a pioneer in synthetic biology who can design vaccines against coronavirus and other pathogens in about an hour. Now, labs can put his methods to work on their own benchtops. Codex

In truth, Gibson had been preparing for this moment for years. His team was already collaborating with Novartis on a grant from the Biomedical Advanced Research and Development Authority (BARDA) on pandemic preparedness. The goal of that project was to create a vaccine against the H1N1 virus. BARDA designed some ambitious, forward-thinking exercises where project members were given an H1N1-type pandemic scenario, a DNA sequence, and a one-week deadline to manufacture a vaccine.

Even in 2013, Gibsons team had shown they could design a vaccine candidate within about 24 hours once given the virus sequence. But they knew that many of the things they were doing in the laboratory could be automated, which would enable others to pursue alternative vaccine candidates in parallel. They had been building a prototype machine that integrated all of their learnings and processes into a single box when H7N9 hit.

This is not a fire drill anymore, Gibson recalls thinking to himself. This is the real deal.

Gibsons team and its prototype system successfully developed an H7N9 vaccine, which Novartis made and the US government stockpiled. Fortunately, the virus did not become a pandemic. But the goal of putting their labs genius into a single box became the origin of a new company and a new product.

The genetic evolution of H7N9 bird virus in China, 2013. Like the novel coronavirus, the H7N9 bird virus probably originated in so-called wet markets. U.S. Centers for Disease Control

Codex DNA(formerly SGI DNA) was launched to make this technology available to researchers everywhere. Codex says that its flagship machine the BioXp 3200 system is the worlds first commercially available, fully automated gene synthesis platform. It synthesizes ready-to-use custom DNA overnight.

The same machine can be used to make DNA to create diagnostic tools, design and develop vaccines, or synthesize the cells to make those vaccines.

The instrument can make up to 32 genes at a time, and it can incorporate downstream processes like assembling the genes into the customers favorite host organism. It can also be used with newer cell-free methods of amplifying DNA to create micrograms of material.

Part of the special sauce in the BioXp system is its error correction process. The Codex team puts specific enzymes in the reagent mix that recognize and remove any unwanted mutations from the population. This allows Codex to synthesize extremely high-fidelity genes, which is critical to applications involving human therapies.

Its not only a research tool, but also a biomanufacturing tool across a wide range of applications. The system streamlines clinical research and allows for distributed manufacturing of personalized therapies, Gibson told me. Its the kind of tool thats needed in hospitals everywhere to not only make brand-new drugs locally, but also to realize the dream of individualized treatments for cancer, rare diseases, and other maladies that humans suffer.

In September, Codex closed a $25 Million Series A financing round to support the commercial launch of the BioXp system, and the company is now focused on making the system accessible to anyone in the research community who has a need for on-demand DNA synthesis across a variety of fields, including vaccine development, antibody drug discovery, personalized medicine, and beyond.

In particular, Gibson and his team are on a mission to get this platform to any researcher working on coronavirus to help in the effort to develop and make therapies and vaccines. There are now hundreds of BioXp systems around the world, with customers now doing in their lab with the BioXp system what Gibson alone was doing back in 2013.

In addition, Codex can provide researchers mostanything they might need to fight the SARS-CoV-2 virus, including positiveSynthetic RNA controlsfor tests, thefull synthetic genomefor vaccine and treatment development, and custom-order antigen panels and antibody librariesall of it made on the BioXp system.

The challenge with coronavirus, says Gibson, who is now the CTO of Codex, is that theres no defined process yet for how you go from your vaccine candidate to a final vaccine. Codexs customers and partners arent immune to that challenge, but the BioXp system is drastically speeding up the process of developing vaccine candidates.

Gibson says of one of his customers: He had ordered reagents from us and six days later he was injecting a vaccine candidate into mice.

All of this is light-years ahead of the current seasonal flu vaccine process, where you typically wait to receive a mucus sample in the mail, isolate the virus, inject it into chicken eggs, and then let them incubate. Thewhole vaccine selection and production processcan take six months, by which time the virus has mutated and your vaccine may not work that well.

Whats so amazing about synthetic biology is that if you have the DNA sequence, you can just download it and start synthesizing it right away, he says.

In a world where infections can travel as fast and far as a jet plane, well need biological defenses that can travel at the speed of the internet.

Follow me on twitter at@johncumbersand@synbiobeta. Subscribe to my weekly newsletters insynthetic biologyThank you toStephanie Michelsenfor additional research and reporting in this article. Im the founder ofSynBioBeta, and some of the companies that I write aboutincluding Codex DNAare sponsors of theSynBioBeta conferenceandweekly digestheres the full list of SynBioBeta sponsors.

Originally published on Forbes: https://www.forbes.com/sites/johncumbers/2020/05/14/a-coronavirus-vaccine-in-six-days/

Originally posted here:
A Coronavirus Vaccine Candidate In Six Days? This Company Is Fast-Tracking Vaccine Development With The First Fully Automated Gene Synthesis Platform...