Monthly Archives: April 2017

No, you can’t avoid taxes by investing in Bitcoin – New York Post

Posted: April 10, 2017 at 2:27 am


New York Post
No, you can't avoid taxes by investing in Bitcoin
New York Post
While many bitcoin aficionados tout the new virtual currency as a promising alternative to so-called fiat currencies like the US dollar, the IRS considers investments in bitcoin as property deals requiring that capital gains or losses in this ...

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Bitcoin Price Analysis – Bitcoin Unlimited support plummets Brave … – Brave New Coin

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Bitcoin has been trading between ~$1075-1200 this week, according to the BLX, on heavy bullish momentum. Total Network Hashrate continues to make all-time highs, despite the ongoing scaling debate. The Network Hashrate is set for a 4.51% rise on about April 12th, increasing 51.89% year to date.

Over the past week, evidence has come forth suggesting why bitcoin mining chip manufacturer Bitmain continues to oppose Bitcoin Improvement Proposal (BIP) 141, Segregated Witness (SegWit). The covert use of AsicBoost provides a 20% competitive advantage towards mining blocks, and is incompatible with SegWit. There is evidence of its existence in current chipsets, but no clear evidence it was used, as the covert form may be completely undetectable. The news preceded is a split in support for Bitcoin Core and Bitcoin Unlimited (BU) this week.

Currently, SegWit and BU support is roughly even, but support for SegWit is expected to grow at a steady pace. SegWits testnet mined a 3.7MB block last week, which is almost 4x the current block size ceiling.

Meanwhile, Japanese exchange volume continues to dominate the global market, led by BitFlyer, which currently trades almost as much volume as all of the USD exchanges combined.

The Ichimoku Cloud is a constant, auto-drawn indicator which quickly offers a trade an immense amount of valuable information on any timeframe. The cloud is best used at higher timeframes as more data generally provides truer signals and less false positives.

The indicator uses moving averages and dynamic support and resistance to make projections of key zones, as well as capturing 80% of any given trend. As long as the price remains above the cloud, sentiment remains bullish. Price in the cloud indicates a neutral trend, and below the cloud indicates a bearish trend.

When the Tenkan (T) is over the Kijun (K) sentiment is bullish. K over T would indicate bearish sentiment. When the Lagging Span (LS) is above the cloud and above the price sentiment is bullish, below the cloud and price would indicate bearish sentiment. The best entry signals for the cloud occur when trend is obvious, but 1 or 2 of the signals have yet to become confluent with a higher timeframe trend.

On the daily timeframe, mixed messages continue for the cloud signals. Price is above cloud but the TK cross remains bearish.

A strong long entry signal would be the TK cross above cloud, with bids on kijun. A similar setup occurred on February 7th. The strong pullback two days later coincided with unfavorable regulatory news out of China.

Another way to measure a longstanding trend is with a pitchfork. There are a few versions of the pitchfork, beginning in 2015, depending on the exchange your viewing. Be sure to use the log scale and pick an extreme low with a swing high and low.

The median line (red) gives the expected mean of the trend. Price will continually attempt to return to this diagonal. Each diagonal of the Pitchfork can be thought of as a potential reversal zone or support/resistance line. The upper blue diagonal zone being most overbought or the top bounds of the trend and lower blue diagonal zone being most oversold or the bottom bounds of the trend.

The trend continues with no threat of stopping anytime soon, with price reaching for a return to mean. Should this trend continue to years end, an expected price of $1800 is reasonable, based on the lower bounds of the pitchfork.

The four hour Heikin-Ashi (HA) candlesticks can be used to assess the strength of an active trend. Heikin-Ashi candlesticks manipulate the raw open and close data from the previous period to better smooth the trend. Stops may be continually moved up based on the bearish candles in a bull trend, a long-wicked candle, or a doji, which would signify a drop in momentum. Entry usually occurs when at least two candles match the active trend following a pullback or stop out.

Depending on how stops are used, each of these trend flip and continuation trades may or may not have been stopped out on each leg up and pullback. Two HA candles are currently green again, but are showing little to no momentum. Until momentum returns, HA is not showing a strong long entry signal, which would likely have limited upside to the local top of the overarching move down.

In the meantime, the previous bullish butterfly harmonic has played out exactly as expected ,with price currently sitting at A-C diagonal resistance.

There is also another bearish harmonic, at the current level, which has formed off the bullish butterfly. Considered an advanced harmonic pattern, the shark is a bearish harmonic with a target of ~$1000-1050. A stop would typically be placed above the 1.272 fibonacci retracement from point C to D, with a short accumulation zone between point D and the 1.272 fibonacci retracement.

Evidence for creating an unfair mining competitive advantage has caused BU support to plummet. All long signals on the Ichimoku cloud have yet to flip bullish, but the longstanding pitchfork shows no threat to trend. HA candles are showing a slowing of bullish momentum at the diagonal resistance of A to C on the completed bullish butterfly harmonic. An advanced bearish harmonic has formed forecasting a target between $1000-1050 in the coming days, should the 1.272 fibonacci extension from points C to D hold as resistance.

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Bitcoin in Gambling Business, What You Need to Know – newsBTC

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Bitcoin is everywhere! Bitcoin continues to take the world by storm. The mainstream media simply does not understand.

Now, Bitcoin is threatening to take over the online gambling business. It is not a surprise, more industries are accepting Bitcoins every week or so. Here is what you need to know about Bitcoin in the gambling business.

When you want to play Blackjack, Poker or Bingo, you put your money down on the table. With online gambling, you deposit your digital funds with the casino. Bitcoin gambling does the very same thing.

Bitcoin is a cryptocurrency. What is that? It is a long word, but all it means is that Bitcoin is a digital currency. Bitcoin is money.

Money is basically anything, which is exchanged for goods and services and fulfills the primary characteristics of money. Some of these features are Store of Value, Medium of Exchange and Fungibility. Bitcoin is money because it has each of these attributes.

Thus, when you want to gamble and need some money, you can use Bitcoin. If you made some Bitcoins, when you painted a fence, then use them to gamble. Everyone needs to vent, from time to time.

Bitcoin is also an amazing store of value. Did you hear that the value of Bitcoin just surpassed gold? Bitcoin is rising in value. How many other forms of money can say the same thing?

You can choose any number of ways to pay for your gambling opportunity credit cards, online payment systems or Bitcoin. Bitcoin has some key advantages because it is an electronic currency. In many ways, Bitcoin mirrors the versatility, speed and beauty of the World Wide Web.

Bitcoin is open. It is created by a community of Web developers who mine the Bitcoins. It has a very relaxed control structure. No nation has a monopoly over Bitcoin.

Bitcoin is rather new. It is a baby of the Internet. As such, it has only been around since 2008. Right now, it is still primarily being promoted by the first adopters. In fact, Bitcoins are still being mined.

Bitcoin is unencumbered. Do you know how many credit card laws, there are? The reason why credit cards take so long to process is because there are so many regulations and companies involved. You dont have that with Bitcoin.

Do you want to gamble quickly? Of course you do. Can you gamble quickly with Bitcoin? Yes.

You can generally deposit and withdraw your funds, faster with Bitcoin. You dont need to wait for the banks to check up on all your financial records. This is a cryptocurrency, meaning that its Blockchain is encrypted to keep you secure.

You can get Bitcoins from your friends, miners or any of the following firms: Kraken.com, BTC-E.com or Localbitcoins.com. Once you own Bitcoins, a whole new world, opens up for you.

Finally, you want to make sure that you find an authentic Bitcoin Casino. You know that there are a vast range of types of websites online. The legitimate Bitcoin Gambling website offers Provably Fair Gambling. What is that?

Provably Fair Gambling is a way to verify the websites authenticity using cryptographic hash functions. This is how the Bitcoin Gambling community is establishing its credibility. So, if you love online gambling, now you can have more fun, by using the efficient electronic currency: Bitcoin.

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NASA’s Peggy Whitson takes command of space station – AOL

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CAPE CANAVERAL, Fla., April 9 (Reuters) - NASA's Peggy Whitson, soon to become the most experienced U.S. astronaut in terms of time spent in space, assumed command of the International Space Station on Sunday as two Russian crew members and an American prepared to fly back to Earth.

For Whitson, 57, it was her second stint in charge of the $100 billion station, a multinational project overseen by the National Aeronautics and Space Administration and the Russian space agency Roscosmos.

SEE ALSO: Jeff Bezos is selling $1 billion of Amazon stock to fund his space rocket company

On April 24, Whitson will have spent more time in space than any other American astronant, surpassing the current U.S. record of 534 days held by NASA's Jeff Williams, 59. She already held records for the most time spent in space by a woman and for the most time spent spacewalking by a woman.

"She will set another record at this moment," the departing U.S. commander, Shane Kimbrough, said during a Sunday change of command ceremony aired on NASA TV. "She becomes the first two-time female commander of the International Space Station."

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The International Space Station (ISS) crew member Peggy Whitson of the U.S., waves before a space suit check at the Baikonur cosmodrome, Kazakhstan, November 17, 2016. REUTERS/Shamil Zhumatov

The International Space Station (ISS) crew member Peggy Whitson of the U.S., walks for space suit check at the Baikonur cosmodrome, Kazakhstan, November 17, 2016. REUTERS/Shamil Zhumatov

The International Space Station (ISS) crew member, Peggy Whitson of the U.S. speaks prior to the launch of Soyuz MS-3 space ship at Baikonur cosmodrome, Kazakhstan, November 17, 2016. REUTERS/Dmitri Lovetsky/ Pool

The International Space Station (ISS) crew member Peggy Whitson of the U.S. is pictured before boarding spacecraft at the Baikonur cosmodrome, Kazakhstan 17 November 2016. REUTERS/Kirill Kudryavtsev/ Pool

The International Space Station (ISS) crew members Peggy Whitson of the U.S., Oleg Novitskiy of Russia and Thomas Pesquet of France walk to board the Soyuz MS-03 spacecraft for the launch at the Baikonur cosmodrome, Kazakhstan 17 November 2016. REUTERS/Kirill Kudryavtsev/ Pool

The International Space Station (ISS) crew member Peggy Whitson of the U.S. tests a space suit during the pre-launch preparations at Baikonur cosmodrome, Kazakhstan 17 November 2016. REUTERS/Yuri Kochetov/ Pool

The International Space Station (ISS) crew members (L-R) Peggy Whitson of the U.S., Oleg Novitskiy of Russia and Thomas Pesquet of France walk from a hotel for a pre-launch preparation at the Baikonur cosmodrome, Kazakhstan November 17, 2016. REUTERS/Shamil Zhumatov

Crew members of the International Space Station (ISS) expedition 50/51 Thomas Pesquet (L) of France, Peggy Whitson (R) of the U.S. and Oleg Novitsky of Russia pose for a picture as they attend an examination training session in Star City outside Moscow, Russia, October 25, 2016. REUTERS/Maxim Zmeyev

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Kimbrough and Russian crewmates Sergey Ryzhikov and Andrey Borisenko were scheduled to wrap up a 173-day mission on Monday, with a parachute landing in Kazakhstan at 7:21 a.m. EDT (1121 GMT).

Their replacements, NASA's Jack Fischer and Fyodor Yurchikhin of Roscosmos, were due to arrive on April 20 at the station, which is in orbit about 250 miles (400 km) above Earth.

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IN SPACE - OCTOBER 7: (EDITORIAL USE ONLY) In this handout photo provided by the European Space Agency (ESA), German ESA astronaut Alexander Gerst takes a photo during his spacewalk, whilst aboard the International Space Station (ISS) on October 7, 2014 in Space. Gerst returned to earth on November 10, 2014 after spending six months on the International Space Station completing an extensive scientific programme, known as the 'Blue Dot' mission (after astronomer Carl Sagan's description of Earth, as seen on a photograph taken by the Voyager probe from six billion kilometres away). (Photo by Alexander Gerst / ESA via Getty Images)

ZHEZKAZGAN, KAZAKHSTAN - MARCH 12: (Alternate crop of #465931716) In this handout provided by the U.S. National Aeronautics and Space Administration (NASA), the Soyuz TMA-14M spacecraft is seen as it lands with Expedition 42 commander Barry Wilmore of NASA, Alexander Samokutyaev of the Russian Federal Space Agency (Roscosmos) and Elena Serova of Roscosmos March 12, 2015 near the town of Zhezkazgan, Kazakhstan. NASA astronaut Wilmore and Russian cosmonauts Samokutyaev and Serova are returning after nearly six months onboard the International Space Station where they served as members of the Expedition 41 and 42 crews. (Photo by Bill Ingalls/NASA via Getty Images)

STAR CITY, RUSSIA - MARCH 5: In this handout from the In this handout from National Aeronautics and Space Administration or NASA, (L to R) NASA Astronaut Scott Kelly is seen inside a Soyuz simulator at the Gagarin Cosmonaut Training Center (GCTC) March 5, 2015 in Star City, Russia. The three are preparing for launch to the International Space Station (ISS) in the Soyuz TMA-16M spacecraft from the Baikonur Cosmodrome in Kazakhstan on March 28, 2015. As the one-year crew, Kelly and Kornienko will return to Earth on Soyuz TMA-18M in March 2016. (Photo by /Bill Ingalls/NASA via Getty Images)

WALLOPS ISLAND, VA - OCTOBER 28: In this handout provided by National Aeronautics and Space Administration (NASA), The Orbital Sciences Corporation Antares rocket, with the Cygnus spacecraft onboard suffers a catastrophic anomaly moments after launch from the Mid-Atlantic Regional Spaceport Pad 0A at NASA Wallops Flight Facility on October 28, 2014 on Wallops Island, Virginia. William Gerstenmaier, associate administrator of NASA's Human Exploration and Operations Mission Directorate and Michael Suffredini, NASA's International Space Station Program Manager also participated in the press conference via phone. Cygnus was on its way to rendezvous with the space station. The Antares rocket lifted off to start its third resupply mission to the International Space Station, but suffered a catastrophic anomaly shortly after lift off at 6:22 p.m. EDT. (Photo by Joel Kowsky/NASA via Getty Images)

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The U.S. and Russian space agencies last week agreed to extend Whitson's mission by three months to fill in as the new crew's third member.

Russia is reducing its station cadre from three to two members until its new science laboratory is launched next year, the head of Roscosmos said at the U.S. Space Symposium in Colorado Springs, Colorado, last week.

Whitson flew to the station in November with Russia's Oleg Novitskiy and France's Thomas Pesquet. The men were scheduled to fly back to Earth without Whitson on June 2.

She was due to return to Earth in September with Fischer and Yurchikhin, having amassed a career U.S. record of more than 665 days in orbit. Russian cosmonaut Gennady Padalka, with 878 days in orbit, is the world's most experienced space flier. (Editing by Frank McGurty and Jonathan Oatis)

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VIDEO: Astronauts on Space Station capture beautiful view of … – NBC4i.com

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COLUMBUS (WCMH)Astronauts traveling in the International Space Station in late March captured a spectacular view of the Northern Lights (auroras) dancing across the polar sky. Auroras are caused when electrically charged solar particles sailing through space collide with oxygen and nitrogen gases in our atmosphere above the polar region.

The dazzling light show is normally confined to high latitudes, but occasionally is seen in the northern United States, and rarely in Ohio.

The view of these glowing auroras was captured by a crew member, who said it was hard to look away from the windows.

Perkins Observatory director Tom Burns, at Ohio Wesleyan University in Delaware, said, We have an atmosphere to protect us. Occasionally, there is a coronal mass ejection of such magnitude that it makes it down as far as Ohio.

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Scientist visits Hazard to "smile" at the International Space Station – WYMT News (press release)

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HAZARD, Ky. (WYMT) - Smiling for the International Space Station...

"I wanted to do something a little bit more than just wave at the camera," Bobby Quimpo who is a scientist said.

Quimpo, who is also a science teacher, contacted the Challenger Learning Center and drove six hours from Lafayette, Indiana to Hazard.

He made the drive in an attempt to get a set of mirrors to reflect back into the camera of the International Space Station.

"It's actually my fourth time, but maybe that's fourth times the charm," said Quimpo.

His first attempt was outside the school he teaches at in July 2016.

Quimpo said, "That was when it had sunny days, perfect, not a cloud in the sky and that was the time they turned off the camera for three hours and I missed the picture."

Another time he missed it because of cloudy skies.

"A lot of trial and error and don't mind trying again, I learn something each time," Quimpo said.

Which is important, as getting the calculations just right is crucial.

"It tells you where the Space Station's going to be, you look up where the sun's going to be and where those angles are you aim the mirror halfway between, you bisect the angle and then put the mirrors on the ground and hope it reflects in," said Quimpo.

Quimpo used ten mirrors to make the smiley face design.

"The largest mirror is two by five feet, most of the mirrors are something like eight inches to a side," Quimpo said.

At exactly 3:25 p.m. and seven seconds on Saturday, he conducted the experiment, but he will not know if it worked for a few days to a few weeks.

"I'll check the image number and see if it actually shows up," said Quimpo.

But he said the experiment is worth the wait.

Quimpo said he used the EarthCam website to conduct his experiment, which allows teachers and students to take pictures from the International Space Station.

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[ April 8, 2017 ] NASA, Roscosmos open to extending station operations to 2028 News – Spaceflight Now

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The exterior of the International Space Station seeing during a spacewalk in September 2016. Credit: NASA

Top officials from NASA and Roscosmos, the Russian space agency, could decide soon to commit to keeping the International Space Station staffed and flying through at least 2028, four years after the research labs current retirement date.

The head of Roscosmos told reporters Tuesday that the Russian space agency is ready to discuss plans to keep operating the huge research complex another four years until 2028.

We think that we should continue working in low Earth orbit, said Roscosmos chief Igor Komarov in a press conference Tuesday at the 33rd Space Symposium in Colorado Springs.

Komarovs comments came after NASAs senior human spaceflight manger, Bill Gerstenmaier, said March 29 that a decision by Congress and the Trump administration whether to commit to continuing space station operations through 2028, one way or another, will create certainty for scientists, engineers and businesses working on the program.

Getting another decision about what we do beyond 2024 with station is really important, Gerstenmaier said in a presentation to the NASA Advisory Councils human exploration and operations committee .

With an eye toward construction of a deep space habitat around the moon in the mid-to-late 2020s, NASA intends to test out new life support systems on the space station that are not as prone to failure and do not require as much maintenance as the technologies currently on the outpost.

NASAs goal is to iron out the kinks of the next-generation life support system, and learn more about how humans respond to long-duration spaceflight, before abandoning the space station and turning attention to deep space exploration.

The life support system on the station today is not of the reliability or the low maintenance that is needed for a Mars-class mission, Gerstenmaier said March 30. We need to really step that up. A great place to test that, in fact the only place to really test that kind of stuff, is on-board the space station.

The Obama administration announced in early 2014 its intention to extend the U.S. commitment to the space station through 2024, a decision that Gerstenmaier lauded as allowing NASA to cement plans to deploy new technology and develop new experiments for the space station.

The decision also helped close the business case for commercial companies working on crew and cargo capsules flying to the space station, giving the service providers a steady stream of business until a potential commercial space station is built in Earth orbit.

If the White House and Congress wait too long extend the space station program, it really limits what the commercial companies are willing to experiment with on space station, Gerstenmaier said. It limits what we need to do with cargo resupply and crew resupply. It changes plans for what we test on station.

The sooner we know that, the better off we are, and waiting until just four years before end of station, I personally think is not as helpful as if we can decide a lot earlier, like soon, Gerstenmaier said.

He added that there is little margin in NASAs schedule to complete the biological and technological experiments needed for deep space missions by 2024.

It took three years for all of the space stations partners to endorse the last extension, with the European Space Agency last year becoming the final participant to lengthen its commitment from 2020 to 2024.

Russia announced in 2015 that it would keep up its support of the space station through 2024, and Komarov said Tuesday that the Russian government will maintain a complex in low Earth orbit throughout the 2020s, whether its the International Space Station or a Russian-led vehicle.

But he implied that Russias preference is to keep the International Space Station going.

As long as we have this instrument, the ISS, its logical to continue this work, Komarov said.

He said the Russian government, like the other space station partners, wants more experiments, more results and more efficiency from the space station.

Roscosmos has a contingency plan that could involve detaching some of its newer modules from the International Space Station, including a research lab set for launch next year, to form a standalone outpost.

It doesnt mean that we dont want to continue our cooperation, Komarov said. We just want to be on the safe side, and in any case, and in any decision, to continue our research in low Earth orbit.

Komarov echoed Gerstenmaiers concerns about using the International Space Station to evaluate astronaut and cosmonaut health and radiation shielding before launching a crewed mission to Mars.

NASA has spent about $67 billion on the space station to date, according to Gerstenmaier. With the contributions of international partners, the orbiting research labs total cost likely reaches above $100 billion.

We ought to be planning, from an policy standpoint, an approach that allows us to maximize the utility of our $67 billion investment in low Earth orbit, and not pick an arbitrary (retirement) date for some other concerns, Gerstenmaier said.

NASA spends more than $3 billion to operate the space station each year, and most of that cost goes toward crew and cargo transportation to and from the complex. The outposts sustaining operating budget is closer to $1 billion per year, Gerstenmaier said.

Engineers have concluded the space station is structurally sound to keep flying through 2028. Some repairs, such as replacement of the research labs oldest power-generating solar arrays, may be required if the program is extended longer than 2028, Gerstenmaier said.

Once officials opt to retire and decommission the station, a series of Russian Progress supply ships will dock with the outpost to gradually lower its orbit, eventually driving the complex to a destructive re-entry over the South Pacific Ocean.

Besides the scientific justification, Gerstenmaier floated two other considerations for U.S. government decision-makers.

Around 15 percent of the global orbital launch attempts in 2015 and 2016 targeted the space station.

Lets say we pick the end, and were now going to pull (15 percent) out of the global launch market. Do you think Im going to be allowed to do that? Probably not.

The other wild card is in 2023 potentially the Chinese will have their space station, Gerstenmaier said. What is the dynamic with the U.S. with a space station thats going away in 2024, with the Chinese having a government-operated space station in 2023? Is that the right time to cede and hand over national and global human spaceflight to another country? You should ponder some of these things.

Email the author.

Follow Stephen Clark on Twitter: @StephenClark1.

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Talking Biotech: Florida neurosurgeon Duane Mitchell on how genetic engineering opens doors in cancer fight – Genetic Literacy Project

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The newest and most promising therapies for challenging cancers are adopting molecular biology strategies. Brain tumors are especially problematic because their location impedes patient function and presents formidable barriers to surgical treatments. But new molecular approaches show promise.

A series of new techniques from viruses genetically trained to attack the tumor to genetic modifications of immune cells to new types of monoclonal antibodies represent a few of the new tricks researchers are now deploying in the fight against these deadly conditions.

Dr. Duane Mitchell is a Professor at the University of Florida. He is a neurosurgeon that is discovering and refining the leading next-generation therapies. In todays podcast, he shares his expertise onthe new types of technologies and the promise they bring.

Website: Dr. Duane Mitchell and the UF Brain Tumor Immunotherapy Program

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Engineering a solution to genetic uniqueness – Grand Junction Daily Sentinel

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By Staff Friday, April 7, 2017

In spite of my hair, no one has ever mistaken me for Einstein. In the gym, after a shower, some people have thought I was Bernie Sanders. But as soon as they started talking to me, they could see that I didnt have his native intelligence.

Science requires no experience, profession, skill or money. Luckily, I was, and am, qualified. If you are a little curious and can think clearly, you can probably do an experiment. I suppose it helps to be a little CDO (which is Obsessive Compulsive Disorder with the letters in the correct sequence). It also helps to be able to focus on a single topic for a long period of time. This requirement has hampered me throughout my career.

Being a scientist probably takes a little money, too, but much less than you might think if you listen to the American Association for the Advancement of Science. Scientists seldom complete all the studies that are possible using a single tool. Every time technology comes up with a new piece of equipment scientists need it to see what they can do with it. Everyone wants the new electric microscopes when we really havent exhausted the old steam-powered microscopes yet.

Science rushes recklessly ahead. Now we have a new method of engineering genetics called CRISPR. This is an acronym for CRISPR/cas9 which stands for Clustered Regularly-Interspaced Short Palindromic Repeats/CRISPR associated protein 9. Id call it CRISPR for sure if I had to say the full name too many times. One could sprain their frenulum or something trying to say all that.

The deal is that CRISPR has decreased the time and costs of genetic engineering by about 99 percent. Generally, reducing time and costs is a great boon to mankind. As a scientist, I am thrilled! However, it just occurred to me that making it faster and cheaper to destroy ourselves may not be in our best interests.

While Monsanto and the two other food monopolies are excited to make more food, now some medical scientists want to eliminate certain neurological and psychiatric diseases that have been the key to producing the great minds of history. Medical scientists seem to see variations in life as problems to be solved. There should be no deviations or abnormalities outside of a normal curve.

However, humans would be smart to notice that genetic variants contribute to psychiatric conditions that may be beneficial depending on ones environment or genetic background. For example, both Thomas Edison and Einstein were considered addled and were kicked out of school. Tennessee Williams feared the process of thought and nearly went mad. The rate of bipolar disorder is 10 times higher among writers than among the general population, and 40 times higher among poets.

I will insert a shameless self-promotion here concerning my book Between Two Mirrors, available on Amazon.

It is a mixture of science essays by a scientist with a spotty record in public school and poetry by the same person, who some continue to believe may have mental problems.

In short, genetic engineering shows a gross misunderstanding of evolution.

Evolution does not progress toward some ideal species where each individual is identical to the others. Instead, it tinkers around the edges of a species toward adaptation to some local niche that itself changes as the species change. It is impossible to predict the result of evolution because the environment selects the species, and the selected species alters the environment. The best one can say is that evolution produces diversity.

Unruly white hair is a characteristic of old, white males of European descent and, in and of itself, is not a sign of genius. However, mental aberrations are not always something to be eliminated. They may be capable of enriching mankind beyond what one might expect. If CRISPR had been available 72 years ago, you might have been spared this column. However, Id have had a lot less fun.

Gary McCallister, .(JavaScript must be enabled to view this email address), is a professor emeritus of biological sciences at Colorado Mesa University.

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How scientists explore our genome’s ‘dark matter’ – Futurity: Research News

Posted: at 2:22 am

A new method lets researchers quicklyscreen the non-coding DNA of the human genome for links to diseases that are driven by changes in gene regulation.

The technique could revolutionize modern medicines understanding of the genetically inherited risks of developing heart disease, diabetes, cancer, neurological disorders, and others, and lead to new treatments.

Identifying single mutations that cause rare, devastating diseases like muscular dystrophy has become relatively straightforward, says Charles Gersbach, the associate professor of biomedical engineering at Duke University. But more common diseases that run in families often involve lots of genes as well as genetic reactions to environmental factors. Its a much more complicated story, and weve been wanting a way to better understand it. Now weve found a way.

As reported in Nature Biotechnology, the new technique relies on the gene-hacking system called CRISPR/Cas9. Originally discovered as a natural antiviral defense mechanism in bacteria, the system recognizes and homes in on the genetic code of previous intruders and then chops up their DNA. In the past several years, researchers have harnessed this biologic system to precisely cut and paste DNA sequences in living organisms.

In the current study, researchers added molecular machinery that can control gene activity by manipulating the web of biomolecules that determines which genes each cell activates and to what degree.

With the new tool, Gersbach and his colleagues are exploring the 98 percent of our genetic code often referred to as the dark matter of the genome.

Only a small fraction of our genome encodes instructions to make proteins that guide cellular activity, says Tyler Klann, the biomedical engineering graduate student who led the work in Gersbachs lab. But more than 90 percent of the genetic variation in the human population that is associated with common disease falls outside of those genes. We set out to develop a technology to map this part of the genome and understand what it is doing.

The answer, says Klann, lies with promoters and enhancers. Promoters sit directly next to the genes they control. Enhancers, however, which modulate promoters, can be just about anywhere due to the genomes complex 3D geometry, making it difficult to discern what theyre actually doing.

If an enhancer is dialing a promoter up or down by 10 or 20 percent, that could logically explain a small genetic contribution to cardiovascular disease, for example, says Gersbach. With this CRISPR-based system, we can more strongly turn these enhancers on and off to see exactly what effect theyre having on the cell. By developing therapies that more dramatically affect these targets in the right direction, we could have a significant effect on the corresponding disease.

Thats all well and good for exploring the regions of the genome that researchers have already identified as being linked to diseases, but there are potentially millions of sites in the genome with unknown functions. To dive down the dark genome rabbit hole, Gersbach turned to colleagues Greg Crawford, associate professor of pediatrics and medical genetics, and Tim Reddy, assistant professor of bioinformatics and biostatistics. All three professors work in the Duke Center for Genomic and Computational Biology.

Crawford developed a way of determining which sections of DNA are open for business. That is, which sections are not tightly packed away, providing access for interactions with biomachinery such as RNA and proteins. These sites, the researchers reason, are the most likely to be contributing to a cells activity in some way. Reddy has been developing computational tools for interpreting these large genomic data sets.

Over the past decade, Crawford has scanned hundreds of types of cells and tissues affected by various diseases and drugs and come up with a list of more than 2 million potentially important sites in the dark genomeclearly far too many to investigate one at a time.

In the new study, Crawford, Reddy, and Gersbach demonstrate a high-throughput screening method to investigate many of these potentially important genetic sequences in short order. While these initial studies screened hundreds of these sites across millions of base pairs of the genome, the researchers are now working to scale this up 100- to 1,000-fold.

Small molecules can target proteins and RNA interference targets RNA, but we needed something to go in and modulate the non-coding part of the genome, says Crawford. Up until now, we didnt have that.

The method starts by delivering millions of CRISPR systems loaded into viruses, each targeting a different genetic point of interest, to millions of cells in a single dish. After ensuring each cell receives only one virus, the team screens them for changes in their gene expression or cellular functions.

For example, someone researching diabetes could do this with pancreatic cells and watch for changes in insulin production. Those cells that show interesting alterations are then isolated and sequenced to determine which stretch of DNA the CRISPR affected, revealing a new genetic piece of the diabetes puzzle.

The technique is already producing results, identifying previously known genetic regulatory elements while also spotting a few new ones. The results also show it can be used to turn genes either on or off, which is superior to other tools for studying biology that only turn genes off. Different cell types also produced differentbut partially overlappingresults, highlighting the biological complexity in gene regulation and disease that can be interrogated with this technology.

Now that we have this tool, we can go in and annotate the functions of these previously unknown but important stretches of our genome, says Gersbach. With so many places to look, and the ability to do it quickly and robustly, well undoubtedly find new segments that are important for disease, which will provide new avenues for developing therapeutics.

The Thorek Memorial Foundation, the National Institutes of Health, and the National Science Foundation supported the work.

Source: Duke University

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How scientists explore our genome's 'dark matter' - Futurity: Research News

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