Genome-wide identification of carbapenem-resistant Gram-negative bacterial (CR-GNB) isolates retrieved from hospitalized patients in Bihar, India |…

Katiyar, A. et al. Genomic profiling of antimicrobial resistance genes in clinical isolates of Salmonella typhi from patients infected with typhoid fever in India. Sci. Rep. 10, 8299 (2020).

ADS CAS PubMed PubMed Central Article Google Scholar

Nordmann, P. & Poirel, L. Epidemiology and diagnostics of carbapenem resistance in Gram-negative bacteria. Clin. Infect. Dis. 69, S521S528 (2019).

CAS PubMed PubMed Central Article Google Scholar

Manandhar, S. et al. A high prevalence of multi-drug resistant Gram-negative bacilli in a Nepali tertiary care hospital and associated widespread distribution of Extended-Spectrum Beta-Lactamase (ESBL) and carbapenemase-encoding genes. Ann. Clin. Microbiol. Antimicrob. 19, 48 (2020).

CAS PubMed PubMed Central Article Google Scholar

Wyres, K. L. et al. Genomic surveillance for hypervirulence and multi-drug resistance in invasive Klebsiella pneumoniae from South and Southeast Asia. Genome Med. 12, 11 (2020).

CAS PubMed PubMed Central Article Google Scholar

World Health Organization. Guidelines for the Prevention and Control of Carbapenem-Resistant Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa in Health Care Facilities (WHO, 2017).

Google Scholar

Zavascki, A. P., Bulitta, J. B. & Landersdorfer, C. B. Combination therapy for carbapenem-resistant Gram-negative bacteria. Expert Rev. Anti. Infect. Ther. 11, 13331353 (2013).

CAS PubMed Article Google Scholar

Karampatakis, T. et al. Molecular epidemiology of endemic carbapenem-resistant Gram-negative bacteria in an intensive care unit. Microb. Drug Resist. 25, 712716 (2018).

PubMed Article CAS Google Scholar

Nair, S. et al. ESBL-producing strains isolated from imported cases of enteric fever in England and Wales reveal multiple chromosomal integrations of blaCTX-M-15 in XDR Salmonella Typhi. J. Antimicrob. Chemother. 76, 14591466 (2021).

CAS PubMed Article Google Scholar

Zagui, G. S. et al. Gram-negative bacteria carrying -lactamase encoding genes in hospital and urban wastewater in Brazil. Environ. Monit. Assess. 192, 376 (2020).

CAS PubMed Article Google Scholar

Elbadawi, H. S. et al. Detection and characterization of carbapenem resistant Gram negative bacilli isolates recovered from hospitalized patients at Soba University Hospital, Sudan. BMC Microbiol. 21, 136 (2021).

CAS PubMed PubMed Central Article Google Scholar

Sawa, T., Kooguchi, K. & Moriyama, K. Molecular diversity of extended-spectrum -lactamases and carbapenemases, and antimicrobial resistance. J. Intensive Care 8, 13 (2020).

PubMed PubMed Central Article Google Scholar

Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: 22th Informational Supplement (CLSI, 2012).

Google Scholar

Altoparlak, U., Aktas, F., Celebi, D., Ozkurt, Z. & Akcay, M. N. Prevalence of metallo-beta-lactamase among Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burn wounds and in vitro activities of antibiotic combinations against these isolates. Burns 31(6), 707710 (2005).

PubMed Article Google Scholar

Song, W. et al. Detection of extended spectrum -lactamases by using boronic-acid as an AmpC -lactamase inhibitor in clinical isolates of Klebsiella spp. and Escherichia coli. J. Clin. Microbiol. 45, 118084 (2007).

CAS PubMed PubMed Central Article Google Scholar

Lee, K. et al. Modified Hodge and EDTA-disk synergy tests to screen metallo--lactamase-producing strains of Pseudomonas and Acinetobactet species. Clin. Microbiol. Infect. 7, 8891 (2001).

CAS PubMed Article Google Scholar

Bolger, A. M., Lohse, M. & Usadel, B. Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics 30, 21142120 (2014).

CAS PubMed PubMed Central Article Google Scholar

Zerbino, D. R. & Birney, E. Velvet: Algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 18, 821829 (2008).

CAS PubMed PubMed Central Article Google Scholar

Gurevich, A., Saveliev, V., Vyahhi, N. & Tesler, G. QUAST: Quality assessment tool for genome assemblies. Bioinformatics 29, 10721075. https://doi.org/10.1093/bioinformatics/btt086 (2013).

CAS Article PubMed PubMed Central Google Scholar

Seemann, T. Prokka: Rapid prokaryotic genome annotation. Bioinformatics 30, 20682069. https://doi.org/10.1093/bioinformatics/btu153 (2014).

CAS Article PubMed Google Scholar

Meletis, G. Carbapenem resistance: Overview of the problem and future perspectives. Ther. Adv. Infect. Dis. 3, 1521 (2015).

Google Scholar

Sands, K. et al. Characterization of antimicrobial-resistant Gram-negative bacteria that cause neonatal sepsis in seven low- and middle-income countries. Nat. Microbiol. 6, 512523 (2021).

PubMed PubMed Central Article CAS Google Scholar

Fonseca, F. et al. The basis for carbapenem hydrolysis by class A -lactamases: A combined investigation using crystallography and simulations. J. Am. Chem. Soc. 134, 1827518285 (2012).

CAS PubMed Article Google Scholar

Satyajeet, K. et al. Carbapenemresistant Enterobacteriaceae: Prevalence and bacteriological profile in a tertiary teaching hospital from rural western India. Indian J. Microbiol. Res. 2020, 7400 (2020).

Google Scholar

Wattal, C. et al. Impact of informational feedback to clinicians on antibiotic-prescribing rates in a tertiary care hospital in Delhi. Indian J. Med. Microbiol. 33, 255259 (2015).

CAS PubMed Article Google Scholar

Gupta, R., Malik, A., Rizvi, M. & Ahmed, M. Presence of metallo-beta-lactamases (MBL), extended-spectrum beta-lactamase (ESBL) & AmpC positive non-fermenting Gram-negative bacilli among Intensive Care Unit patients with special reference to molecular detection of bla(CTX-M) & bla(AmpC) genes. Indian J. Med. Res. 144, 271275 (2016).

CAS PubMed PubMed Central Article Google Scholar

Rohit, A. et al. CTX-M type extended-spectrum & #946;-lactamase in Escherichia coli isolated from extra-intestinal infections in a tertiary care hospital in south India. Indian J. Med. Res. 149, 281284 (2019).

CAS PubMed PubMed Central Article Google Scholar

Muzaheed, et al. High prevalence of CTX-M-15-producing Klebsiella pneumoniae among inpatients and outpatients with urinary tract infection in Southern India. J. Antimicrob. Chemother. 61, 13931394 (2008).

CAS PubMed PubMed Central Article Google Scholar

Devi, L. et al. Increasing prevalence of Escherichia coli and Klebsiella pneumoniae producing CTX-M-type extended-spectrum beta-lactamase, carbapenemase, and NDM-1 in patients from a rural community with community acquired infections: A 3-year study. Int. J. Appl. Basic Med. Res. 10, 156163 (2020).

CAS PubMed PubMed Central Article Google Scholar

Khan, E. R. et al. Prevalence and molecular epidemiology of clinical isolates of Escherichia coli and Klebsiella pneumoniae harboring extended-spectrum beta-lactamase and carbapenemase genes in Bangladesh. Microb. Drug Resist. 24, 15681579 (2018).

CAS PubMed Article Google Scholar

Rocha, F. R., Pinto, V. P. T. & Barbosa, F. C. B. The spread of CTX-M-type extended-spectrum -lactamases in Brazil: A systematic review. Microb. Drug Resist. 22, 301311 (2015).

PubMed Article CAS Google Scholar

Xia, S. et al. Dominance of CTX-M-type extended-spectrum -lactamase (ESBL)-producing Escherichia coli isolated from patients with community-onset and hospital-onset infection in China. PLoS ONE 9, e100707e100707 (2014).

ADS PubMed PubMed Central Article CAS Google Scholar

Abrar, S. et al. Distribution of blaCTX M, blaTEM, blaSHV and blaOXA genes in extended-spectrum--lactamase-producing clinical isolates: A three-year multi-center study from Lahore, Pakistan. Antimicrob. Resist. Infect. Control 8, 80 (2019).

PubMed PubMed Central Article Google Scholar

Zeynudin, A. et al. Prevalence and antibiotic susceptibility pattern of CTX-M type extended-spectrum -lactamases among clinical isolates of gram-negative bacilli in Jimma, Ethiopia. BMC Infect. Dis. 18, 524 (2018).

CAS PubMed PubMed Central Article Google Scholar

Yuki, S. et al. Environmental presence and genetic characteristics of carbapenemase-producing enterobacteriaceae from hospital sewage and river water in the Philippines. Appl. Environ. Microbiol. 86, e01906-e1919 (2021).

Google Scholar

Marie-Frdrique, L. et al. Extended-spectrum -lactamases of the CTX-M type now in Switzerland. Antimicrob. Agents Chemother. 51, 28552860 (2007).

Article CAS Google Scholar

Quinteros, M. et al. Extended-Spectrum -lactamases in enterobacteriaceae in Buenos Aires, Argentina, Public Hospitals. Antimicrob. Agents Chemother. 47, 28642867 (2003).

CAS PubMed PubMed Central Article Google Scholar

Al Naiemi, N. et al. Widely distributed and predominant CTX-M extended-spectrum -lactamases in Amsterdam, The Netherlands. J. Clin. Microbiol. 44, 30123014 (2006).

CAS PubMed PubMed Central Article Google Scholar

Zhao, W.-H. & Hu, Z.-Q. Epidemiology and genetics of CTX-M extended-spectrum -lactamases in Gram-negative bacteria. Crit. Rev. Microbiol. 39, 79101 (2013).

CAS PubMed Article Google Scholar

Pokhrel, R. H., Thapa, B., Kafle, R., Shah, P. K. & Tribuddharat, C. Co-existence of beta-lactamases in clinical isolates of Escherichia coli from Kathmandu, Nepal. BMC Res. Notes 7, 694 (2014).

PubMed PubMed Central Article CAS Google Scholar

Laurent, P., de la Ortiz, R.J.-M., Anas, R., Marta, A.-S. & Patrice, N. CTX-M-33 Is a CTX-M-15 derivative conferring reduced susceptibility to carbapenems. Antimicrob. Agents Chemother. 63, e01515-19 (2021).

Google Scholar

Walsh, T. R. Emerging carbapenemases: A global perspective. Int. J. Antimicrob. Agents 36, S8S14 (2010).

CAS PubMed Article Google Scholar

Nachimuthu, R. et al. Characterization of carbapenem-resistant Gram-negative bacteria from Tamil Nadu. J. Chemother. 28, 371374 (2016).

CAS PubMed Article Google Scholar

Kazi, M. et al. Molecular characterization of carbapenem-resistant Enterobacteriaceae at a tertiary care laboratory in Mumbai. Eur. J. Clin. Microbiol. Infect. Dis. 34, 467472 (2015).

CAS PubMed Article Google Scholar

Okoche, D., Asiimwe, B. B., Katabazi, F. A., Kato, L. & Najjuka, C. F. Prevalence and characterization of carbapenem-resistant enterobacteriaceae isolated from Mulago National Referral Hospital, Uganda. PLoS ONE 10, e0135745 (2015).

PubMed PubMed Central Article CAS Google Scholar

Mushi, M. F., Mshana, S. E., Imirzalioglu, C. & Bwanga, F. Carbapenemase genes among multidrug resistant gram negative clinical isolates from a Tertiary Hospital in Mwanza, Tanzania. Biomed Res. Int. 2014, 303104 (2014).

PubMed PubMed Central Article CAS Google Scholar

Hornsey, M., Phee, L. & Wareham, D. W. A novel variant, NDM-5, of the New Delhi metallo--lactamase in a multidrug-resistant Escherichia coli ST648 isolate recovered from a patient in the United Kingdom. Antimicrob. Agents Chemother. 55, 59525954 (2011).

CAS PubMed PubMed Central Article Google Scholar

Liang, W. et al. Emergence and mechanism of carbapenem-resistant Escherichia coli in Henan, China, 2014. J. Infect. Public Health 11, 347351 (2018).

PubMed Article Google Scholar

Kopotsa, K., Sekyere, J. O. & Mbelle, N. Characterization of plasmids mediating carbapenem-resistance in Klebsiella pneumoniae in Pretoria, South Africa. Int. J. Infect. Dis. 101, 14 (2020).

Article Google Scholar

Mmatli, M., Mbelle, N. M., Maningi, N. E. & Osei Sekyere, J. Emerging transcriptional and genomic mechanisms mediating carbapenem and polymyxin resistance in enterobacteriaceae: A systematic review of current reports. mSystems 5, e007830 (2020).

Article Google Scholar

Rawat, D. & Nair, D. Extended-spectrum & #223;-lactamases in gram negative bacteria. J. Glob. Infect. Dis. 2, 263274 (2010).

PubMed PubMed Central Article Google Scholar

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Genome-wide identification of carbapenem-resistant Gram-negative bacterial (CR-GNB) isolates retrieved from hospitalized patients in Bihar, India |...