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Category Archives: Cloning

Not Entirely in His DNA: Gilles Simon Criticizes Grigor Dimitrov for Cloning the Style of Ro … – EssentiallySports

Posted: December 6, 2020 at 10:46 am

French professional tennis player Gilles Simon seems quite fascinated with Roger Federer. Even when he doesnt want to talk about the Swiss Maestro, journalists find a way to ask him something about Federer. However, he respects the 20-time Grand Slam winner and has admitted earlier that Federer was his sons hero.

In a recent interview, there was a question about the former World No.3 Grigor Dimitrov. However, the interviewer phrased it in a manner that it also involved Federer. The question was whether Dimitrov had limited himself in his career by replicating Federer. To this, the Frenchman replied in affirmative.

Yes, began a confident Simon. Hes (Grigor Dimitrov) clearly a player who comes close in terms of fluidity of play.

It was clear that Simon felt that Dimitrov was quite close in cloning Roger Federers style. He further believed that it was something that was not doing any good for Dimitrov.

Read More: Gilles Simon racks up Roger Federer again in the debate on tennis role model

Simon further argued that Dimitrov was trying to do something that he simply couldnt do.

In the important moments, it can give up because, in reality, it is not entirely in his DNA, concluded the 35-year-old Simon.

He was blunt and didnt hesitate in saying that Federers style was not there in Dimitrovs DNA. He said that the player could try to copy it but it was futile as it would not help him in the longer run.

It was certain that Simon was criticizing Dimitrov. In a way, he even advised him not to do so in the future.

Both Dimitrov and Simon will begin the upcoming season at the Australian Open 2021. Joining them there will be none other than Roger Federer who will also make a comeback to tennis after a year.

Also Read: Gilles Simon slams French Tennis for wasting energy on replicating Roger Federer

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Stream On: A parade of clones, and the Uncanny Valley – The Outer Banks Voice

Posted: at 10:46 am

By Peter Hummers on December 3, 2020

They call themselves sisters, but the truth is stranger than that; they are a biotech experiment gone sideways, and now in big trouble. (IMDb.com)

We may be as gods, as the serpent told Eve in the Garden of Eden; were not gods, really, but were close. We cant create matter, but we can create tools by assembling matter. We can create life, but biologically, through reproduction, like any other organism. We long to create life as a god does. Mary Shelley put the kibosh on the notion of reanimation with her story Frankenstein; Or, the Modern Prometheus, but we continue to strivethrough cloning, andif were not carefulartificial intelligence.

Leda and the Swan is a story from Greek mythology in which the god Zeus, in the form of a swan, seduces Leda. She bore Helen and Pollux, children of Zeus, while at the same time bearing Castor and Clytemnestra, children of her husband Tyndareus, the King of Sparta. And so Helen and Clytemnestra were twin half-sisters, and Castor and Pollux were twin half-brothers.

Orphan Black begins as Sarah Manning, a con artist and drug trafficker, witnesses the suicide of a woman, Beth Childs, who appears to be her double. Sarah assumes Beths identity, apartment and occupation after Beths death. During the first season, Sarah discovers that she and Beth are clones, and that she has many sister clones spread throughout North America and Europe that are all part of an illegal human cloning experiment, and that someone is plotting to kill them.

Orphan Black is a deep rabbit-hole: Beth had been a detective, and Sarah must use her street smarts to get over with Beths partner, superiors and boyfriend. She finds out that she as Sarah was wanted by the police; that the biotech firm that spawned them has planted covert watchers in the girls personal lives (except, presumably, Sarah, who was raised as an orphan); and that members of a religious movement are using another clone to find and assassinate the others, whom they regard as abominations.

Sarah meets three more of her sisters, via one of Beths two cellphones, and they eventually discover a group of male clones in the miltary (Project Castor). But Sarahs first urgent concern of many is the question of why Beth killed herself.

Lavishly produced and well-written, this exciting and fun roller-coaster ride is wholly owned by Tatiana Maslany [IMDb.com], who portrays all of the female clones, each as a very different persona scientist, a cop, a soccer mom, etc. She shines especially when portraying one clone posing as another. Maslany won a Primetime Emmy Award, a TCA Award, two Critics Choice Awards, and five Canadian Screen Awards. [Heres] a trailer.

Anita (center) is an exemplary maid and companion to the Hawkins family. Anita is a household appliance; the latest in synths, and of course, not what she seems. Problem is, Anita is not even what it is supposed to be: an unthinking machine. (IMDb.com)

More than 40 years ago, Masahiro Mori, a robotics professor at the Tokyo Institute of Technology, wrote an essay on peoples reactions to robots that looked and acted almost human. In particular, he hypothesized that a persons response to a humanlike robot would abruptly shift from empathy to revulsion as it approached, but failed to attain, a lifelike appearance. This descent into eeriness he called the uncanny valley.

After missing his wife at home in his busy household, Joe Hawkins buys a pretty synth (Gemma Chan, Crazy Rich Asians), a robotic assistant that looks like a young woman; he doesnt consult his wife. Upon her return, his wife Laura feels displaced and cast off. She also complains that this will confuse the children, especially after the youngest child, Sophie, names the robot Anita after her friend who moved away.

In a flashback, a group of self-aware synths including Leo, Max, Niska, and Anita are hiding out in the forest five weeks earlier; everyone except Max and Leo are abducted and taken away into London [Wikipedia].

Pinocchio, the sentient puppet, became a real boy, fascinating readers of the 1883 stories and children ever since. In the 1959 Twilight Zone episode The Lonely, an exile falls in love with an android; in the 1960 episode The Lateness of the Hour, an android believes she is human. Most famous are the replicants of Blade Runner, who aspire to humanity and make the human protagonistand the audiencewonder about his own identity.

Humans touches all these bases, beginning with the exquisite creepiness of the uncanny valley, and extending into the companionship felt by a lonely retiree (William Hurt) and his attempts to save his buggy older-model synth from recycling as mandated by the U.K. Health Service. A small, secret group of sentient synths, created as an experiment, plot to upload their consciousness to the others and lead a rebellion, and one, a sex worker, kills a client and escapes her bordello, intending to violently initiate the revolution.

The series is so well done that we dont think about Pinocchio or Blade Runner, even when one synth reassures her owners of their safety by reciting Asimovs [Three Laws of Robotics] from I, Robot. Humans holds a Tomatometer rating of 89% and an audience rating of 91% from [Rotten Tomatoes]. [Heres] a trailer.

Next time, Little Red Riding Hood, as told by Alfred Hitchcock and Orson Welles.

(Pete Hummers is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to earn fees by linking Amazon.com and affiliate sites. This adds nothing to Amazons prices.)

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Rick & Morty Theory: Space Beth Is Real Beth | Screen Rant – Screen Rant

Posted: at 10:46 am

Rick and Morty season 4's introduction of Space Beth raised the question of which Beth is a clone, and we think Space Beth is the real one.

Rick and Morty season 4brought the original Beth and clone Beth face to face, and even though no one in the show knows who is the real Beth, there is reason to believe "Space Beth" is the real one. The idea of cloning Beth came duringRick and Morty season 3. Beth was in the middle of a breakup with Jerry and sought adventure, but she put her future in Rick's hands.However, it wasn't until season 4 thatRick and Morty provided any exploration of what happened.

Two versions of Beth were featured inRick and Morty's season 4 finale, "Star Mort Rickturn of the Jerri." After a full season of Beth reunited with her family, Space Beth arrived as a cosmic warrior who spent her time battling the New Galactic Federation. Space Beth believed that she wasa clone after she discovered an implant in her neck,prompting her to return back to Earth to kill Rick. However, she learns that both Beths have such implants, adding to the confusion of who is real and who is a clone. Although both Beths were interested in learning who was real and who was the clone at first,Rick and Morty ended season 4with the entire family, except Rick, left in the dark. He begins toreveal his "Mind Blower" of the event (a recorded memory), which shows him cloning Beth, but then removing the labels from the cloning vats and rotate their placements repeatedly so he wouldn't know which Beth was real.

Related: Rick & Morty Theory: Beth Is Smarter Than Rick

SinceRick and Morty didn't provide any answers on which Beth is real and which is a clone,a fanbase built on wild theories has been left to create some more. The truth behind the two versions of Beth is one of the topics that has continued to be debated since season 4 ended, with cases to be made for either Beth being the clone. Since they were exactly the same at the end of the cloning process and Beth's uncertainty about what she wanted,the future for both Beths could just come down to where Rick put them.That said, one of them is definitively a clone, and even if Rick and Morty never answers the question,there's reason tobelieve that Space Beth is the real, or at least original Beth.

Space Beth's trajectory as a character falls in line with what Beth wanted for herself, based on her discussion about cloning with Rick. Sheexpressed a desireto go have more adventures after spending time in Froopyland with Rick and realizing that she's more like her father than she thought. Space Beth being the real Beth means she finally got the chance to break free of her mundane life and find happiness elsewhere. Although this would mean that the real Beth also abandoned her family, it was already fractured at this time. This arc gives Beth some agency in her story, instead of simply coming to a realization that she was happy all along and just didn't realize it. Plus, it's not like this would be the first time a Sanchez left their family behind to pursue their own interests, so this reveal would further illustrate how similar Beth and Rick are.

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Jurassic Park: Why Frog DNA Was Used to Create The Dinosaurs – Screen Rant

Posted: at 10:46 am

Jurassic Park's science uses frog DNA is a notable addition to the incomplete genome of a dinosaur, and it also gives them very special abilities.

The originalJurassic Parkfilmreveals that frog DNA was used to help create the dinosaurs, causing a problem with InGen's plan to limit breeding. Released in 1993, Steven Spielberg's classic not onlyboastedground-breaking advancements inCGI technology, but it also explored thescientific phenomena of cloning. While the logistics are not fully explained in the movie, they still provide a compellinglookinto the reemergence of dinosaurs.

When paleontologist Dr. Alan Grantand the team first arrive at the Jurassic Park Visitor Center, they are treated to a tour by John Hammond, founder of the park and of InGen, the company which makes dinosaur cloning possible. Their first stop includes a filmnarrated by Mr. DNA, the animated helix, who explains how the dinosaurs came to be. In simplified terms, scientists were able to extract dinosaur blood from prehistoric mosquitos preserved in amber. Because most of the samples' genomes were incomplete, geneticists needed something to, as Mr. DNA says, "fill in the holes and complete the code." This is where frog DNAcamein.

Related: Jurassic Park: All 6 Dinosaurs That Appear In The First Movie Explained

Frog DNA serves as both an easy, uncomplicated solution to the dinosaurs' genetic sequence and a plot device for later on in the film. It's that DNA solution that is responsible foreverydinosaur in the Jurassic Park series, like the T-Rex and Velociraptor before the later movies move into more overt genetic splicing. It'scombinedwith the dinosaurs' DNA sequence to make a finalized version for fertilization, filling in the gaps from degradation over the thousands of years since their extinction. In the film, it's only frog DNA that was used to serve the plot and explain how some dinosaurs have been able to change sex, while the original book uses a number of additional options. Fundamentally, it serves two other purposes too. Not only does it foreshadow the franchise's genetic tinkering and establishes that Dr Henry Wu's morals come second to his scientific drive,while adding an accessible means to explain how the amber extraction method can possibly lead to full sequence DNA cloning.

Michael Crichton's novel, on which the movie is based, provides a complex analysis of the differentchicken and amphibian genomes used in creating and boosting the growth of dinosaurs.Jurassic Park'sfilmmakers likely wanted to makethe process as simple as possible for audiences to understand, sofrog DNAbecame the sole additionalinsertion into the dinosaurs' biological makeup. Frog DNAis mentioned again in a later scene, when Dr. Grant, Lex, and Tim,discover freshly-hatched eggs outside of the lab. According to Dr. Wu, the head geneticist of the park's lab, all dinosaurs are programmed to be female, so breeding should be impossible. Upon finding the eggs, Dr. Grant remarks that some West African frogscan alter their sex in a single-sex space.Bymarrying a frogs genetic code with the dinosaurs', scientists gave dinosaursthe frogs ability to change sex and, therefore, mate. However, what type of frog DNA was actually used and whether the scientists knew about this trait from their research remains unclear.

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Top 5 Most Heartbreaking Moments in ‘The Clone Wars’ – Nerds and Beyond

Posted: at 10:46 am

Its been exactly seven months since Star Wars: The Clone Wars released its final episode, and we still arent over the fact that the beloved story is over. The seven season long animated series shows the untold stories of the Star Wars prequels, capturing the hearts of fans everywhere. There were happy moments, shocking moments, moments that angered you and moments that made you laugh, but most of all, the emotional moments made this series all the better. Were breaking down the top 5 most heartbreaking moments in The Clone Wars.

5. Satine dying in Obi-Wans arms

Season 5, Episode 16, The Lawless

Image Courtesy Disney +.

Our fifth favorite heartbreaking moment involves the beloved Jedi Knight Obi-Wan Kenobi and the leader of Mandalore Duchess Satine Kryze. During The Clone Wars, we learn that Obi-Wan and Satine have a history together they once loved one another. Obi-Wan goes as far as saying if Satine asked him to, he wouldve left the Jedi Order for her.

The news of conflict on Mandalore between the planets government, Death Watch a terrorist splinter group of Mandalorians, and a criminal organization found by Darth Maul the Shadow Collective, reached Obi-Wan and he managed to infiltrate the capital city of Mandalore to save Satine. In an attempt to escape, Satine and Obi-Wan were captured and brought to Maul. Due to the long and complicated history between the Sith and the Jedi, Maul declares he wants Kenobi to feel his pain before stabbing Satine with the Darksaber. In the arms of her lover, Satine uses her dying breath to express her eternal love to Obi-Wan.

Remember, my dear Obi-Wan, Ive loved you always I always will. -Satine Kryzes final words to Obi-Wan Kenobi

This moment is so sad because of the fact that we finally learn about Obi-Wans true feelings toward the Duchess. Before this moment, Star Wars fans never knew of Obi-Wan possessing any emotions of that nature, and now that we do, he loses her. Knowing the outcome of Revenge of the Sith, the viewer now sees that Obi-Wan has truly lost everyone he has ever loved.

4. 99 dying like the hero he is

Season 3, Episode 2, ARC Troopers

Image Courtesy Disney +.

Coming in at number four, we have an incredibly moving moment that will pull at your heartstrings. One of the best things about The Clone Wars is that while it focuses on Anakin, Obi-Wan, Ahsoka, and the Jedi Order, it also shares the stories of the millions of clones that fans have come to love. A repeated theme within the series when it comes to the clones is that while they may look the same, they are all unique. One of the first clones we see who is truly unique is 99.

99 is a malformed clone who was deemed unfit to serve in the Grand Army of the Republic during The Clone Wars because his physical abilities were limited and his faster-than-normal rapid aging. He was instead assigned to work janitorial duties in the cloning facilities. 99 befriended the Domino Squad while they were still cadets, and later reunited with Echo and Fives when they came back to the facility. While there, an attack ensues and being the brave soul that he is, 99 joins the soldiers to help defend the city. He fought for his brothers, and during an act of heroism, 99 was shot and killed by a battle droid. Because of his sacrifice to help save the city, his brothers commended 99 as a true soldier.

We lost a true soldier.He really was one of us. Commander Cody and Captain Rex mourning 99

This moment can break even the toughest hearts. It truly shows the viewers as well as the other clones that no matter how different one may seem, they can be the most caring, loyal, and brave of them all. 99 may have only been in three episodes, but his legacy and sacrifice lives on. Four seasons later, we see a motley crew of clones who are unique like 99, fighting in battles and defending The Republic. Their squad name? Clone Force 99.

3. 501st dying during Order 66

Season 7, Episode 12, Victory and Death

Image Courtesy Disney +.

This was a huge moment within The Clone Wars. It was the moment Star Wars fans have been waiting for since the first season Order 66. Instead of following the moments of Anakin during this infamous turn of events, we follow Ahsoka and the 501st Battalion whom we have grown to love throughout the series.

In the last days of The Clone Wars, Anakin splits up the 501st Battalion by sending a group of the soldiers to Mandalore with the 332nd Company to assist Ahsoka in liberating the planet from Mauls rule. During their journey back to Coruscant, Palpatine declares Order 66. The clones then turn on Ahsoka. In a stressful moment, she gets the inhibitor chip out of Captain Rexs head, returning him back to normal and the two try to escape while the men she considered her family were in full attack mode.

Youre a good soldier, Rex. So is every one of those men down there. They may be willing to die, but I am not the one who is going to kill them. -Ahsoka Tano talking to Captain Rex before facing the 501st during Order 66

After a long and intense battle, Ahsoka and Rex escape off of the falling Star Destroyer where the rest of the 332nd and 501st clones were. Later, Rex lands the Y-wing they escaped in near the Star Destroyers crash site, and in a beautifully heartbreaking moment, Ahsoka lays down her lightsabers by the makeshift graves the two made for each of the fallen clones.

We have always know what happened with the group of 501st clones that stayed with Anakin during Order 66: they stormed the Jedi Temple and killed those that lived there, including Jedi younglings, under the leadership of the new Sith Lord Darth Vader. However, this final moment with some of the most beloved clones in the series breaks your heart knowing that they were made to fight a battle they were designed to lose.

2. Fives dying while trying to expose Palpatine

Season 6, Episode 4, Orders

Image Courtesy Disney +.

Our number two pick is truly so important in the grand scheme of things. Without this happening, future stories within the galaxy could be extremely different. While season 7 of the series showed what happened during Order 66, the season before touched on the subject through the clone trooper, Fives.

During a military campaign, Tup another clone trooper and close friend of Fives killed a Jedi general, completely unaware of what he did. Fives accompanies his friend to Kamino to figure out what is happening. Fives quickly grows suspicious of the cloning facility and investigates on his own. Fives was sent to Coruscant to undergo further tests after telling Jedi Master Shaak Ti about the chip he discovered. Once on Coruscant, Palpatine reveals the truth behind Order 66 to the troubled clone before attacking him. In a moment of self-defense, Fives was framed as a potential assassin and went on the run. Anakin and Rex meet with Fives to get to the bottom of whats going on, however Fivess attempts to explain what he knows proves incoherent, and in a desperate attempt to get them to listen, Fives reaches for Rexs blasters but before exposing the truth, he was shot by another clone.

Thisitsbigger than any of usthan anythingI couldve imagined.I never meant toI only wanted to do my duty. ARC Trooper Fives to Captain Rex before he dies

Rex and Ahsoka are able to survive Order 66 because of Fives. Rex was able to tell her to find Fives moments before trying to kill her. Looking through archives, Ahsoka finds a file on Fives and learns about the inhibitor chip, allowing her to save Rex. This moment is truly so sad. Fives was scared and agitated that no one was listening to him, and without being able to convince others of what he knows, he died in his brothers arms.

1. Ahsoka leaving the Jedi Order

Season 5, Episode 20, The Wrong Jedi

Image Courtesy Disney +.

And finally, coming in at number one for our top five most heartbreaking moments is a monumental scene that changed the course of the Star Wars story as we know it Ahsoka leaving the Jedi Order.

Bariss another Jedi padawan and friend of Ahoska sets off a bomb in one of the Jedi temples due to her distain toward the Jedi Council and the war itself, and frames the beloved Togruta for the crime. Ahsoka was labeled a traitor and terrorist, however she was able to escape from imprisonment to set off in hopes of clearing her name. Her master, Anakin, never lost hope and ended up proving her innocence during her trial before the Council. Although her name was cleared, Ahsoka couldnt dismiss the fact that the Jedi Council didnt trust her, and this prompted her to leave.

Im sorry, Master, but Im not coming back. Ahsoka Tano to Anakin Skywalker

The tearful goodbye is truly felt by the audience. Ahsoka doesnt want to leave and Anakin doesnt want her to. Its gut-wrenching. This heart-shattering moment not only paves the way for her own path, it does the same for Anakins. Ahsoka giving up her found family she made within the Jedi Order is thought to be one of the contributing factors into why Anakin went to the dark side becoming Darth Vader.

These are just some of the most heartbreaking moments in The Clone Wars. There are several others that can shatter your heart just as easily. You can stream all seven seasons of the hit show on Disney+ now.

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Critical steps outlined with property transfers and trust cloning | nestegg – nestegg.com.au

Posted: at 10:46 am

Speaking at the CGW Virtual SMSF Intensive Day, Cooper Grace Ward Lawyers associate Keeghan Silcock explained that in Queensland, there is no specific duty concession available where a client is looking to transfer an asset from a member into an SMSF.

However, it may be possible in certain circumstances to have a transfer from a related trust into an SMSF with no duty payable, Ms Silcock said.

She gave an example of a property owned in Queensland by a family trust that the client wants to transfer into super.

We have a property which is owned in Queensland by the Trump Family Trust and were wanting to get that into super, so we set up a new SMSF, the Top Trumps SMSF, and we have Donald and Melania as the sole two members with equal member balances and thats really important because Donald and Melania are also the sole income and capital default beneficiaries of the family trust, she explained.

What that means is that they are the only two people holding trust interests in both of these two trusts, the family trust and the family SMSF.

Ms Silcock explained that a strategy can be used where Trump Pty Ltd declares that it ceases holding the property in Queensland as trustee for the family trust, and then commences holding that as trustee for the Top Trumps SMSF.

In this example, we do that by having Trump Proprietary Limited acting as trustee of both of these trusts, but it is possible to do this where there are different trustees you just need to include an additional step of having the SMSF appoint the other trustee as its custodian for the purposes of holding that property, she stated.

Due to the way the duty legislation in Queensland operates, this is not a dutiable transaction and so if its done properly, there is no duty payable in Queensland on this change.

If we do this with Trump Proprietary Limited acting as trustee of both the trusts, it is possible to change the trustee for one of those entities at a later date, but we dont suggest doing that in the short term because it can trigger anti-avoidance issues.

Speaking in the same session, Cooper Grace Ward Lawyers partner Clinton Jackson said if the right steps are taken and everything is dealt with appropriately, there wont be compliance issues with this strategy.

With the trust cloning, if you were to immediately take steps that would defeat the trust cloning, there is a risk that the anti-avoidance provisions would apply, he warned.

In our experience though, most of our clients deal with this in a more sensible way and theyre not looking to change trustees or go too close to the line, and weve never had anyone have any issues with the anti-avoidance provisions with these types of transactions.

Mr Clinton also pointed out that where SMSF professionals are looking to use exemptions to move property into super in NSW and Victoria, they still need to be conscious of section 66 of the SIS Act.

[This] means that if were acquiring something from a related party, it needs to be business real property, so we cant do residential, and it also needs [to be] at market value, he said.

Now market value could be one of two things; it could be a payment from the fund to that person, and in NSW, that would be OK, but in Victoria, its not.

The other way to sort out a market value payment, he said, is for the fund to accept an in-specie contribution and therefore increase the value of the fund.

That would effectively satisfy the market value requirements of section 66 because the fund has taken an increase and the member has made that as a contribution, he said.

So, there are definitely ways to deal with it. Its just a matter of making sure we structure those transactions properly, and our documentation is really key.

Critical steps outlined with property transfers and trust cloning

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Global Cloned Competent Cells Market Projections, SWOT Analysis, Risk Analysis And Forecast By 2020- – PharmiWeb.com

Posted: November 29, 2020 at 6:19 am

Global Cloned Competent Cells Market 2020 by Company, Regions, Type and Application, Forecast to 2025 is a detailed and dedicated analysis of the present scenario of the market covering the various aspects applicable to business growth. The report evaluates the market, putting forth all-inclusive data that enriches the scope, understanding, and application of this report. The report encompasses the information on the global Cloned Competent Cells markets status. The report will offer wide-ranging information segregated into the diverse section that can further simplify the understanding of the market dynamics. The research entails various details about the market size, trends, share, cost structure, growth, revenue, capacity, and forecast. All the players actively participating in the global market are covered.

NOTE: Our analysts monitoring the situation across the globe explains that the market will generate remunerative prospects for producers post COVID-19 crisis. The report aims to provide an additional illustration of the latest scenario, economic slowdown, and COVID-19 impact on the overall industry.

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The report also discusses recent developments and product portfolios of the key players. The global market report delivers a comprehensive overview of the markets global development including its features and forecast. The market is deeply evaluated by a current market situation such as market growth factors and constraints. The report includes a detailed explanation about the past and present trends of the market that has been following along with its future analysis. The report highlights highly competitive areas of the global Cloned Competent Cells market and details the mapping of the vendor landscape.

The key and emerging market players in the global market include: Merck KGaA, Yeastern Biotech, Takara Bio, Thermo Fisher Scientific, GeneScript Corporation, Agilent Technologies, QIAGEN N.V., Beijing TransGen Biotech, Promega Corporation, New England Biolabs, Bioline, OriGene Technologies, Bio-Rad Laboratories, Lucigen, Zymo Research

The product types covered in the report include: 20*100_l, 50*100_l, 100*100_l, Other

The application types covered in the report include: Subcloning & Routine Cloning, Phage Display Library Construction, Toxic/Unstable Dna Cloning, High-Throughput Cloning

This report also shows global Cloned Competent Cells market import/export, supply, expenditure illustrations as well as cost, price, industry revenue, and gross margin by regions covering: North America (United States, Canada and Mexico), Europe (Germany, France, UK, Russia and Italy), Asia-Pacific (China, Japan, Korea, India and Southeast Asia), South America (Brazil, Argentina, etc.), Middle East & Africa (Saudi Arabia, Egypt, Nigeria and South Africa)

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PRIA WARS: Attack of the DeFi Clones – hackernoon.com

Posted: at 6:19 am

@h1ddenzer0h1ddenZer0

A long-time crypto nerd working as part of the team for the new DeFi machine, PRIA.

A few weeks ago PRIA was released.

PRIA exploded out of the gates, immediately pumping to ~$8 before making a much needed correction, with price dropping down to ~$1.50.

For a few days there was a lull.

Price consolidated in the $23 range, and after some substantial marketing efforts from the team, the project was picked up by Crypto Twitter and Telegram influencers. PRIA became the talk of the town and started to pump AGAIN.

It was #1 on Dextools continually, and over the course of a few days the price pumped from $2 to $82.

Each time we woke in the morning, the PRIA price had risen by $1520!

It was exciting. It was euphoric. Lambo dealerships smashed pre-order records. Some investors went to the moon. Some to mars. Some tore a hole in the fabric of spacetime and hopped thru a wormhole. The money making machine showed no signs of stopping.

But then, something happened. The inevitable happened. Hell emptied itself of evil and PRIA suffered from The Attack of the Clones.

PRIAs trading volume was juicy and the clones came to feast.

As mentioned, this was inevitable and expected. The DeFi space is filled with clones. Many of these clones come without any innovation whatsoever. These low quality clones seek to vampirically leach on the success of the project theyre cloning by capitalising in on the hype of the moment.

For obvious reasons, DeFi clones without innovation often die a rapidly painful death, or, become rug pulls. And though some early clone investors make big gains, many people lose everything. It is NOT worth the risk.

The following is what happened to the PRIA clones.

Though clones are expected in DeFi, the first clone is always the most painful. And this was no exception, the first PRIA clone, ARIA, stung a lot.

The ARIA team cloned absolutely everything, including the layout of the website AND the logo! Everything. The only changes they made were:

To add to the irritation, one of the main orchestrators of the ARIA clone attack was a fairly vocal and trusted member of the community Kevin C, who is an Admin for a legit project some of us really like: Contribute.

I wonderwhy!?!?

Prior to the clone, we were a little suspicious of Kevin C as he mentioned that he missed out on investing early in PRIA, yet he still hung around in our Telegram channel, always with comments that resembled gentle FUD.

As he was the Admin of Contribute we gave him a free pass (trustworthy), which was a mistake.

Beware of fudders, folks. They are resentful people, often jealous because they didnt make the money they wished, and they will harm your project. Best case scenario is that they annoy people in your channel. Worst case scenario is that they clone your whole project.

And so it happened with ARIA.

Naturally, we were pissed and took a number of actions to discredit their project and protect our own. PRIA WARS: Attack of the Clones began, and the PRIA Jedi gathered together and headed to the frontline for war.

As a first counter attack, Jedi Master Dr Mantis (PRIA creator) triggered the manager killswitch function on their project.

Since they copied the code verbatim and didnt change dates nor contract addresses, Jedi Master Mantis was able to immediately stop their code from being editable (full Defi, baby!) by interacting with their smart contract and triggering the manager killswitch. This resulted in a liquidity leak, and ARIA was burned every time trades were made with Uniswap.

Next step was rather bold, and some might say, brash. But, as the saying goes: all is fair in love and war and this was war!

We, the PRIA Jedi, banned everyone from our PRIA Telegram channel who had joined the ARIA Telegram channel, and Jedi Master Mantis blacklisted any ethereum addresses that had interacted with the ARIA smart contract.

This caused quite the stir.

People did NOT like being removed from the PRIA Telegram channel, but an example needed to be set. People soon realised that the PRIA Jedi do NOT play games.

Classic degens, unaware of why we might bepissed.

Next, the PRIA Jedi took to Twitter and Telegram and, pardon my language, fudded the fuck out of them. They had unforgivably taken a significant portion of our trade volume and we were determined to disrupt their initial success and get it back.

Every single Twitter post they made was immediately hit with a plethora of negative comments from the PRIA Jedi, and similarly in their Telegram.

It worked.

Regular people who werent the PRIA Jedi organically started asking whether ARIA was a scam in their Telegram. We were winning.

ARIA did attempt to fight back a couple of times on Twitter

Some minor, yet futile, resistance.

but it was futile. The PRIA Jedi were simply too strong and spammed the above post with factual and brutally honest FUD concerning the ARIA project.

As one would expect, this rather quickly led to the demoralising of ARIA Telegram channel members

And ARIA started to die a painful death with volume dropping sharply.

The PRIA Jedi conquered, and ARIA is no more.

A price chart fromHell.

After ARIA, the Attack of the Clones continued, and war intensified. Multiple clones every single day were created.

ORIA, KRIA, VRIA, ZRIA, TRIA, RRIA, BRIA, yPRIA, pizzeRIA, DRIA, nDRIA, etc, etc I mean, WTF!!

Many of them followed the same model as ARIA, by cloning everything and changing very little. Most are too boringly similar to call out individually.

Though DRIA did have an interesting logo

Hahahaha! With a logo like that, DRIA wasnt a cause forconcern.

Initially our strategy remained the same: Attack them head on. Ban them, blacklist them, FUD them, trigger the killswitch.

But it quickly became apparent that, although we are PRIA Jedi Knights, we didnt have the bandwidth to fight them all. We adapted our strategy, moving towards automation and turning the clones on themselves.

In addition to the bandwidth issues, we also realised that since the clones were also competing with each other, and each clone community has essentially the same degenerate ethos, it wouldnt be too long before they started cannibalising themselves, leaving PRIA to reign supreme.

For automation, Jedi Master Mantis wrote an alert system that analysed all smart contract diffs. If the contract was similar to PRIA, it would immediately execute the killswitch to ensure a liquidity leak and an eventual death.

For our FUD efforts, instead of generically fudding, we started to directly FUD the clones by promoting other clone projects! We did this in the Twitter and Telegram channels of the separate clone projects.

This catalysed the clone cannibalism we hoped.

Soon members from each of the clone projects started fudding each other, leading the eventual destruction of them all.

First it started with regular old sweary FUD

Then the discourse became a little more colourful

"Goats", yousay?

Even Admins started berating each other. Heres our good friend Kevin C again arguing with another Admin, KDOT, but this time with a different clone project: nDRIA!

Gotta give it to Kevin C, he knows how to pick a shitty project!

And finally, our favourite: A beautiful crescendo of porn spamming

The PRIA Jedi won the first wave of war.

After the 710 day Attack of the Clones, things slowed down a little bit and the clones were attacking with less frequency.

Witnessing the total clone destruction from the PRIA Jedi, some clones regrouped, ready to launch another attack.

This attack came in the form of a clone 2.0 named Oscillate. They had put in some effort beyond a straight copy and paste of the code. They seemingly meant business.

Oscillate actually launched their OWN uniquely designed website and significantly changed the name. And as all the other clones did, they made some rudimentary modifications to some of the math regarding the burn rate and maximum supply.

This clone 2.0 was going places. Lol.

Their OWN website.Wow!

If any clone had a chance of becoming successful it felt like it would be this one.

In addition their website and name, they had a ton of degens shilling in their Telegram. They reached an impressive ~1000 members within a day! And they also fixed the contract code to stop us from triggering the killswitch.

However, rather hilariously, after a few hours any concerns we had about Oscillate were alleviated upon noticing that one of the Admins of their Telegram group was a braindead fudder from our own PRIA Telegram channel a week earlier!

When we were pumping hard in the previous week, with price going from $2 to $82, an imbecilic degen named Lord Fes came into the PRIA Telegram and started calling everyone cunts and spamming masturbating-Pepe and Nazi-Pepe memes. According to him, he did this because we mooned without him.

Ladies and gentlemen, I introduce the singular brain-celled, Lord Fes

Lord Fes isnt the sharpest tool in theshed.

As I mentioned. Be careful with fudders and deal with them swiftly. Lord Fes lasted about 60 seconds in our channel.

As expected, any project that would appoint Lord Fes as an Admin would not last for long, and this was no exception. Oscillate died a horrible death, just like all the rest.

See?

Another price chart fromHell.

After countless battles and a week or so of intense combat, it felt that PRIA WARS: Attack of the Clones had reached its conclusion

The good guys (us) had won.

But, no.

There was a last hurrah. We came up against a new clone, PRIAx.

PRIAx was created by another of our previous community members, Dominik. For a few weeks Dominik had been a member of the PRIA Telegram channel, and he frequently complained and fudded. We were too tolerant, however, and we warned him numerous times and continued to keep him around. Every so often he would intersperse his FUD with positivity and optimism, and that was enough to forgive him for a few hours.

And, to be honest, it really seemed like he was just a very emotional (perhaps bipolar) guy whose mood was exponentially correlated with the price of PRIA. If the PRIA price went down slightly, Dominiks mood crashed worse than the Wall St Crash of 1929. If PRIA price went up slightly, his mood elevated like hed just triple dropped whilst clubbing in Ibiza.

His mood swings resulted in him insinuating that the project was a scam and other such FUD. And eventually, after a few days of negative PRIA price action, he couldnt contain himself. His mood crashed and he repeatedly fudded.

He didnt heed our warnings, and continued

The community didnt like it, we received multiple requests to ban him. We felt wed been lenient enough, and so we muted him to give us all a break for a couple of days.

That break didnt come, however

As the next day, PRIAx emerged. Dominik was the Admin and he claimed that PRIAx was actually created by us! Check it out

Well, this was hilarious! Loyalty rarely exists in the DeFi world.

So, I repeat: Be very wary of fudders, folks. They lack emotional restraint and are prone to destructive behaviour. They will hurt your project.

We knew PRIAx wouldnt be successful. This may sound like arrogance, but it was obvious. The PRIAx website was also selling COVID testing kits!

A few days later, Dominik sent one of the PRIA Jedi a direct message to apologise, but the damage had already been done.

Regardless, we wish Dominik the best in life.

Thats all, folks! (For now!)

But just wait for the next time PRIA pumps! A second wave of clones will come, this time stronger than before, again in an attempt to dethrone the king.

They forget that there is only one PRIA.

PRIA WARS: Attack of the Clones taught us a lot and showed us just how robust PRIA actually is. The 1 million test simulations prior to launching the PRIA project certainly paid off, because, PRIA Jedi aside, the minor modifications the clones made to the math would NOT have worked anyway.

To compete with PRIA, the clone must reinforce and weaponise itself with purposeful and meticulous innovation. Perhaps such a clone will emerge in the future...

Stay tuned for the sequel: PRIA WARS 2: Return of the Clones.

If youd like to explore the PRIA protocol you can visit the website and join our thriving Telegram community.

Create your free account to unlock your custom reading experience.

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PRIA WARS: Attack of the DeFi Clones - hackernoon.com

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Competent Cells Market size, share, trends and industry analysis investigated in the latest research – WhaTech

Posted: at 6:18 am

According to research report the competent cells market is projected to reach $2.22 billion by 2022, at a CAGR of 10.2%.

According to research report the global competent cells market is projected to reach USD 2.22 billion by 2022 from USD 1.37 billion in 2017, at a CAGR of 10.2%.

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Advancements in molecular cloning research due to the emergence of new technologies and increasing commercial demand for molecular cloned products and recombinant proteins are the major factors driving the growth of the competent cells market.

North America to dominate the competent cells market in 2017

By region, the global competent cells market is segmented into North America, Europe, Asia-Pacific, and the Rest of the World (RoW). In 2017, North America is expected to account for the largest share of the market.

This can be attributed to the increasing intensity of research due to technological advancement and increasing commercial needs for genomic and protein expressed products, in North American countries.

By application, the cloning segment is expected to dominate the market in 2017

Based on application, the competent cells market is broadly segmented into cloning, protein expression, and other applications. Cloning is expected to command the largest share of this market in 2017.

Increasing research on cloning, driven by government support and funding is among the major factors driving market growth in this segment.

The cloning application is further segmented into subcloning & routine cloning, phage display library construction, toxic/unstable DNA cloning, and high-throughput cloning. Similarly, other applications of competent cells include mutagenesis, single-stranded DNA production, lentiviral vector production, and large plasmid transformation.

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By type, the chemically competent cells segment is expected to dominate the market in 2017

Based on the type of competent cells, the market is segmented into chemically competent cells and electrocompetent cells. The growth of the chemically competent cells segment can be attributed to the rising intensity of research (due to technological advancement) and availability of government support, along with the growing market demand for molecular cloned products.

By end user, the academic research institutes segment is expected to dominate the market in 2017

On the basis of end user, the competent cells market is segmented into academic research institutes, contract research organizations (CROs), and pharmaceutical & biotechnology companies. In 2017, the academic research institutes segment is expected to account for the largest share of the market.

The growth of this end-user segment is driven by the increase in research funding for life science projects worldwide.

Key players operating in the competent cells market include Merck KGaA (Germany), Promega Corporation (U.S.), Thermo Fisher Scientific, Inc. (U.S), New England Biolabs (U.S.), Takara Bio (Japan), Agilent Technologies (U.S.), Lucigen (Epicenter) (Illumina) (U.S.), QIAGEN N.V.

(Germany), OriGene Technologies (U.S.), Bioline (U.K.), Zymo Research (U.S.), Beijing TransGen Biotech Co., Ltd. (China), Source BioScience (U.K.), IBA GmBH (Germany), Genewiz (U.S.), BioDynamics Laboratory, Inc.

(Japan), GCC Biotech (India), SMOBIO Technology (Taiwan), GeneScript Corporation (U.S.), Bio-Rad Laboratories (U.S.), Life Technologies (India) Pvt. Ltd., Cell Applications, Inc. (U.S.), Delphi Genetics (Belgium), Scarab Genomics, LLC (U.S.), and Yeastern Biotech Co., Ltd.

(Taiwan).

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Competent Cells Market size, share, trends and industry analysis investigated in the latest research - WhaTech

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Orphan Black: All 17 Clones Tatiana Maslany Played In The Show – Screen Rant

Posted: September 21, 2020 at 7:01 pm

Orphan Black star Tatiana Maslany played 14 different clones over the show's five seasons, all with the same face but radically different looks.

BBC sci-fi series Orphan Blackisn't just a gripping story of illegal experiments in human cloning- it also featured an acting tour de force by star Tatiana Maslany. The Canadian actress played five main characters throughout the series, and appeared in many "guest" roles as other clones that they encountered. In the Orphan Black series finale it was revealed that there were 276 Leda clones created in total, and Maslany played 14 of them on-screen.

Created by Graeme Manson and John Fawcett, Orphan Black's story begins with petty criminal Sarah Manning arriving on a train platform and encountering a woman who looks exactly like her. While Sarah is still in shock, the woman commits suicide by stepping in front of a train. Over the course of the next five seasons, Sarah follows the dead woman down a rabbit hole that reveals her own true origins, and puts both her and her daughter in grave danger.

Related:How Orphan Black Season 5 Ended The Brilliant Series

With Maslany now cast back into the spotlight with the announcement that she'll play the title role in Marvel's She-Hulk Disney+ series, here's a look back at the many differentLeda clones she played in Orphan Black.

Sarah is one of only two clones that escaped Neolution's watchful gaze. Her birth mother, Amelia, escaped before giving birth to her twins. She gave one to the church and one to the state, and Sarah was the twin who went to the state. She ended upin the care of Siobhan Sadler, who moved Sarah and her foster brother, Felix, to America in order to keep Sarah out of the clutches of people who were searching for her. Sarah got her love of punk rock from Siobhan,who sharply pointed out that Sarah "took all of the attitude, none of the politics." from the music. Sarah is the only Leda clone who was able to conceive a child naturally - a miracle, given that the clones were deliberately made sterile - and she loves her daughter Kira intensely.

Police detective Beth Childs was part of the original "Clone Club" alongside Alison Hendrix and Cosima Niehaus, and was using her skills to help piece together the mystery of the clones and the illness that was afflicting some of them. Beth's investigation led her to cross paths with the Proletheans, a group of religious extremists who consider the clones to be abominations. This culminated in Beth killing a Prolethean called Maggie Chen, resulting in her suspension and an internal investigation into the civilian shooting. Between the trauma of the shooting and her unrequited love for her boyfriend and monitor, Paul, Beth's mental health took a downward spiral that eventually took her to the train platform.

Commonly referred to as "The German," poor Katja Obinger's appearance in Orphan Black was short-lived. The newest addition to Clone Club, Katja flew all the way to Canada with documents and samples from clones in Europe. She was suffering from the respiratory illness that afflicted many of the Leda clones, and hoped that with her sisters' help she could find a cure. She soon saw through Sarah's pretense of being Beth, but before she couldfind out who she was really talking to, Helena ended Katja's life with a sniper bullet to the brain.

Related:15 Science Fiction TV Shows You Need To Binge Watch

Among the files that Katja brings with her are the IDs of several clones around Europewho had already been assassinated by Helena. Aryanna Giordano was a Leda clone raised in Italy, and little else is known about her.

Another of the IDs that Katja brings with her is that of Janika Angler, a clone who was born in Salzburg, Austria. Like Aryanna, Janika is dead before Orphan Black begins, having been assassinated by Helena.

The final European clone assassinated by Helena is Danielle Fournier, who lived in Paris, France. Like the previous two, Danielle only appears in an ID photo and not much is known about her - except that it must have taken some work to get that lovely head of curly hair, given that the clones have naturally straight hair.

Alison Hendrix is the third fellow clone that Sarah meets in the flesh, and she's quickly disillusioned ofany belief that soccer moms aren't scary. Alison is married to her monitor, Donnie, and they have two children that they adopted. Alison is part of the original Clone Club - and since she didn't have police access like Beth or science expertise like Cosima, she contributed to the sisterhood by giving Beth a $75,000 "self-defense fund." Alisonis desperate to just lead a normal life and hates being a clone so much that she tries to forbid use of the word in Clone Club. The name of the club gives a good indication of how successful Alison's efforts were.

Related:Orphan Black: How Many Leda & Castor Clones Were Created

Hippie scientist Cosima leads the charge on trying to find a cure for the clones' mysterious illness, which becomes all the more urgent when she herself starts coughing up blood. Studying for herPh.D. in Experimental Evolutionary Developmental Biology (or "Evo-Devo," as she prefers to call it), Cosima acts as a mediating force between the hot-headed Sarah and the tightly-wound Alison. However, even Cosima lets her emotions get the better of her when she suspects that the attractive Frenchwoman trying to befriend her, Delphine, is a monitor sent by Neolution to keep an eye on her. Cosima's argument that she could learn something by getting close to Delphine and doing a bit of reverse-monitoring is motivated by something a little more primal than the scientific method.

In Orphan Black season 1, Sarah learns from her birth mother that the psycho clone stalking and assassinating the others is actually her twin, Helena. Amelia gave Helena to the church and she ended up in the hands of the Proletheans, who weaponized her against her own sestras. At the start of the series Helena has been brainwashed into thinking that she's the original and all the other clones are aberrations that must be eliminated. Despite her early villainous behavior, however, she ends upbecoming a core member of Clone Club and finding a real family.

Most of the Leda clones were kept oblivious to their true nature (unlike their Castor brothers, who were raised in the military and are self-aware). Rachel Duncan is the exception: a child raised by Neolution. She takes her last name from the scientists Ethan and Susan Duncan, who developed the LEDA and CASTOR cloning projects and brought her up as their own daughter in an apparently loving home. Rachel serves as an example of what the Leda clones strengths - Cosima's intelligence, Sarah's defiance, Alison's fierceness when threatened, and Helena's killer instinct - look like when they take a truly villainous form.

Introduced in Orphan Black season 4 and expanded upon in the comic book series Orphan Black: Helsinki,Veera "M.K." Suominen (referred to affectionately as "Mika" by the Clone Club) is revealed to have been working with Beth to uncover the conspiracy behind the clones. M.K. was hit harder by Beth's suicide than perhaps any of the other clones, as Beth was one of the few people in her life she could trust. Later, M.K. reaches out to the remaining clones to warn them that Neolution is catching up to them.An adept computer hacker, she prefers to communicate via webcam while wearing a sheep mask. Like Cosima, M.K. contracts the respiratory illness that affects so many of the Leda clones. Sadly, she's murdered before Cosima is able to find the cure.

A teacher and swim coach, Jennifer Fitzsimmons was the first of the Leda clones to show signs of the respiratory illness that also affected Cosima and Katja. Cosima is introduced to Jennifer in Orphan Black season 2 via a series of video diaries thatshekept from the start of her illness all the way up until her death. For Cosima, Jennifer is a vision of her own future - one that she gets uncomfortably close to when she performs an autopsy on the deceased clone.

Related:Interview With Orphan Black's Tatiana Maslany

Though she might seem like a dumb blonde stereotype, manicurist Krsytal Goderitch proves that she has the same drive, boldness, and (admittedly misguided) intelligence as her sisters. After being attacked by Castor clones Rudy and Seth, Krystal comes to realize that there's a conspiracy afoot and starts independently investigating it. Admittedly she gets the details of the conspiracy wrong and decides that it's all part of corruption in the beauty industry, but she got the general picture of powerful people working in the shadows correct. Like the other cloneswho make a living appearance in Orphan Black, Krystal Goderitch is eventually introduced to one of her doubles and told she is a clone. Unlike the other clones, she flat-out dismisses the clone story as nonsense on the basis that Sarah looks nothing like her ("she's like a seven on a good day, and I've been told I'm a ten").

Tony Sawickiis a transgender clone who appears in just one episode of Orphan Black, after his monitor is gunned down and asks him to pass on a message to Beth Childs. This leads Tony to Art, who takes him to Felix's place. A long day of verbal sparring and flirtation ensues, in which it becomes clear thatTony is cut from the same cloth as Sarah in terms of personality as well as genes. Tony is remarkably calm when he finally learns that he's a clone, only commenting on how hot all of the clones are. With the Dyad Institute on his trail, Tony is instructed to get on a bus and get as far away as possible. He's also given a Clone Club phone and told tocall if he ever gets in trouble. Since he never calls, we can optimistically assume that he made a clean escape and started a new life elsewhere.

Miriam is another clone who is only introduced after she's already died. Raised by an alcoholic mother, Miriam ended up living on the streets. She was a musician with a powerful creative streak, but she was unfortunate enough to attract the attention of Rachel when Miriam developed uterine growths. Unwilling to wait for Miriam to die of natural causes, Rachel killed her so that she could examine her body - a callous act that shocked even Aldous Leekie.

In a flashback in Orphan Black season 5, we see the first clone that Helena ever killed. Unfortunately, Helena doesn't stop to ask her name. The sestra is praying in a church when Helena comes up from behind and stabs her in the back. Afterwards, she is visibly shaken to see her own face staring back up at her from the dying woman.

The very last clone to be introduced in Orphan Black, Camilla Torres appearstowards the end of the series finale. She also has the respiratory illness, but is able to receive the cure from Delphine. Cosima is present and eavesdrops while Camilla receives the cure, but chooses to turn her head away when her new sister leaves, leaving her unaware of her clone status.

More: TheMost Anticipated Sci-Fi TV Shows of 2020

Cobra Kai Theory: What Happened To Robby After Season 2 Finale

Hannah has been with Screen Rant since the heady days of 2013, starting out as a humble news writer and eventually clawing her way up the ladder through a series of Machiavellian schemes and betrayals. She's now a features writer and editor, covering the hottest topics in the world of nerddom from her home base in Oxford, UK.Hannah enjoys weird horror movies, weirder sci-fi movies, and also the movie adaptation of Need for Speed - the greatest video game movie of all time. She has lived and studied in New York and Toronto, but ultimately returned home so that she could get a decent cup of tea. Her hobbies include drawing, video games, long walks in the countryside, and wasting far too much time on Twitter.Speaking of which, you can follow Hannah online at @HSW3K

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Orphan Black: All 17 Clones Tatiana Maslany Played In The Show - Screen Rant

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