Daily Archives: April 2, 2017

Space station crew cultivates crystals for drug development – Phys.org – Phys.Org

Posted: April 2, 2017 at 7:37 am

March 31, 2017 by Jenny Howard Crystal formation within a 50 millimeter loop, taken on Expedition 6. Crystal growth investigations have been occurring on the station since before humans lived there because of the unique environment microgravity provides. Credit: NASA

Crew members aboard the International Space Station will begin conducting research this week to improve the way we grow crystals on Earth. The information gained from the experiments could speed up the process for drug development, benefiting humans around the world.

Proteins serve an important role within the human body. Without them, the body wouldn't be able to regulate, repair or protect itself. Many proteins are too small to be studied even under a microscope, and must be crystallized in order to determine their 3-D structures. These structures tell researchers how a single protein functions and its involvement in the development of disease. Once modeled, drug developers can use the structure to develop a specific drug to interact with the protein, a process called structure-based drug design.

Two investigations, The Effect of Macromolecular Transport on Microgravity Protein Crystallization (LMM Biophysics 1) and Growth Rate Dispersion as a Predictive Indicator for Biological Crystal Samples Where Quality Can be Improved with Microgravity Growth (LMM Biophysics 3), will study the formation of these crystals, looking at why microgravity-grown crystals often are of higher quality than Earth-grown, and which crystals may benefit from being grown in space.

Rate of Growth - LMM Biophysics 1

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Researchers know that crystals grown in space often contain fewer imperfections than those grown on Earth, but the reasoning behind that phenomenon isn't crystal clear. A widely accepted theory in the crystallography community is that the crystals are of higher quality because they grow slower in microgravity due to a lack of buoyancy-induced convection. The only way these protein molecules move in microgravity is by random diffusion, a process that is much slower than movement on Earth.

Another less-explored theory is that a higher level of purification can be achieved in microgravity. A pure crystal may contain thousands of copies of a single protein. Once crystals are returned to Earth and exposed to an X-Ray beam, the X-ray diffraction pattern can be used to mathematically map a protein's structure.

"When you purify proteins to grow crystals, the protein molecules tend to stick to each other in a random fashion," said Lawrence DeLucas, LMM Biophysics 1 primary investigator. "These protein aggregates can then incorporate into the growing crystals causing defects, disturbing the protein alignment, which then reduces the crystal's X-ray diffraction quality."

The theory states that in microgravity, a dimer, or two proteins stuck together, will move much slower than a monomer, or a single protein, giving aggregates less opportunity to incorporate into the crystal.

"You're selecting out for predominantly monomer growth, and minimizing the amount of aggregates that are incorporated into the crystal because they move so much more slowly," said DeLucas.

The LMM Biophysics 1 investigation will put these two theories to the test, to try to understand the reason(s) microgravity-grown crystals are often of superior quality and size compared to their Earth-grown counterparts. Improved X-ray diffraction data results in a more precise protein structure and thereby enhancing our understanding of the protein's biological function and future drug discovery.

Crystal Types - LMM Biophysics 3

As LMM Biophysics 1 studies why space-grown crystals are of higher quality than Earth-grown crystals, LMM Biophysics 3 will take a look at which crystals may benefit from crystallization in space. Research has found that only some proteins crystallized in space benefit from microgravity growth. The shape and surface of the protein that makes up a crystal defines its potential for success in microgravity.

"Some proteins are like building blocks," said Edward Snell, LMM Biophysics 3 primary investigator. "It's very easy to stack them. Those are the ones that won't benefit from microgravity. Others are like jelly beans. When you try and build a nice array of them on the ground, they want to roll away and not be ordered. Those are the ones that benefit from microgravity. What we're trying to do is distinguish the blocks from the jelly beans."

Understanding how different proteins crystallize in microgravity will give researchers a deeper view into how these proteins function, and help to determine which crystals should be transported to the space station for growth.

"We're maximizing the use of a scarce resource, and making sure that every crystal we put up there benefits the scientists on the ground," said Snell.

These crystals could be used in drug development and disease research around the world.

Explore further: First-class protein crystals thanks to weightlessness on earth

Dutch chemist Paul Poodt has developed two attractive alternatives for allowing protein crystals to grow under weightless conditions. If the crystals are grown upside down in a strong magnetic field, fluid flows that disrupt ...

The tenth SpaceX cargo resupply launch to the International Space Station, targeted for launch Feb. 18, will deliver investigations that study human health, Earth science and weather patterns. Here are some highlights of ...

Twinning is a crystal-growth disorder in which the specimen is composed of distinct domains whose orientations differ but are related in a particular, well-defined way. Twinning, which is a known problem in protein crystallography, ...

(Phys.org) Innovative methods of drug discovery do not always take place in an academic laboratory. They may start there, but they can also happen in orbit aboard the International Space Station, as protein crystallization ...

On April 18, 2014, former astronaut and UAB Professor Lawrence DeLucas, O.D., Ph.D., stood at Cape Canaveral and watched several hundred crystallization experiments blast into orbit aboard the SpaceX Falcon 9 rocket. Then ...

Crystals that don't experience mechanical stress during growth have superior quality. Levitating liquid metal is the idea behind the project 'Perfecting metal crystals' led by the University of Twente in the Netherlands.

Magnetic reconnection, a universal process that triggers solar flares and northern lights and can disrupt cell phone service and fusion experiments, occurs much faster than theory says that it should. Now researchers at the ...

Calculating a proton's spin used to be an easy college assignment. In fact, Carl Gagliardi remembers answering that question when he was a physics graduate student in the 1970s. But the real answer turned out not to be simple ...

Crew members aboard the International Space Station will begin conducting research this week to improve the way we grow crystals on Earth. The information gained from the experiments could speed up the process for drug development, ...

The electron microscope, a powerful tool for science, just became even more powerful, with an improvement developed by Cornell physicists. Their electron microscope pixel array detector (EMPAD) yields not just an image, but ...

Researchers from the ARC Centre for Ultrahigh bandwidth Devices for Optical Systems (CUDOS) in the University of Sydney's Australian Institute for Nanoscale Science and Technology have made a breakthrough achieving radio ...

Most people have never seen an accelerometera device that measures change in velocityand wouldn't know where to look. Yet accelerometers have become essential to modern life, from controlling automobile airbags, to ...

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The $190 billion orbiting white elephant that killed the Shuttle and so many space missions has never yielded anything of value.

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Forget Google Moonshots, Alphabet to Open Data Center in Mars Next Year – 1redDrop (blog)

Posted: at 7:36 am

Google should have been the cloud industry pioneer, but Amazon pulled the rug from right under them and raced to the top position, leaving all the tech giants breathless as they try and catch up. But now, Google Cloud Platform has suddenly been resurrected in a big way (not that it died, but it was fairly dormant) and plans are afoot to expand their data center footprint to new regions. And by regions, we mean planets. Of course.

By 2018, Google wants to have a data center operational on Mars, the Red Planet voted in high school as most likely to succeed in providing a home to earthlings. And its not an April Fools Day joke either.

Essentially, Alphabet wants to expand its compute, network and storage power to Mars, first for the purpose of data processing and storage for Mars rovers, and then in preparation of the colonization of Mars. Over time, they intend to move massive amounts of data and make it redundant with data stored in more earthly locations.

Theyve already got a nickname for the worlds first interplanetary data center Ziggy Stardust This region will also serve as an important node in an extensive network throughout the solar system, says Google on it cloud blog.

Theyre really serious about it. I think. This is what theyre saying:

In order to ease the transition for our Earthling customers, Google Cloud Storage (GCS) is launching a new Earth-Mars Multi-Regional location. Users can store planet-redundant data across Earth and Mars, which means even if Earth experiences another asteroid strike like the one that wiped out the dinosaurs, your cat videos, selfies and other data will still be safe.

Huh? Whos actually going to watch those videos if my and my cat are both wiped out? Thats what Id like to know, Larry.

Google also quotes one of its early access customers:

This will be a game changer for us. With GCS, we can store all the data collected from our rovers right on Mars and run big data analytics to query exabyte-scale datasets all in a matter of seconds. Our dream of colonizing Mars by 2020 can now become a reality.

I wonder who that customer is. Musk be somebody we know, probably.

The Google Planets team has already identified a site for the data center Gale Crater, near the landing site of NASAs Curiosity rover.

Why just Mars, you ask? Google has the answer to that:

But why stop at Mars? Were taking a moonshot at N+42 redundancy with galaxy-scale computing. While GCP is optimized for faster-than-light data coordination for databases, the Google Planets team is already hard at work mapping the rest of our solar system for future data center locations.

This could possibly be the moonshot-est moonshot that Google has ever come up with, but colonizing Mars is becoming a high priority item in several board rooms across the United States. If we succeed in getting even a small colony set up on Mars by 2020, at least they wont have to worry about not having access to cat videos.

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The Colonization Of Mars – MediaPost Communications

Posted: at 7:36 am

Yesterday humanity marked a glorious milestone: SpaceX successfully relaunched and re-landed a Falcon 9 -- the first time in history a rocket has been reused.

Reusing rockets is a big deal. In his superb deep dive into all things Elon Musk, Wait But Why author Tim Urban provides a useful analogy: Imagine the current air travel industry with one key difference: an airplane works for one flight only. Each flight is on a brand new plane, and after the flight, passengers exit into the terminal and the plane is broken down into scrap metal and possibly-reusable parts that are sent off to be refurbished for use in a future plane.

An airplane costs around $300 million to build. So in this new model, in addition to paying for the crews time and fuel, airlines have to spend $300 million extra each flight to build a plane. How would that change things?

First, there would be very few flights availablethe schedule would be limited by the pace of plane production. Second, the price of a round-trip ticket between Chicago and San Francisco would now cost about $1.5 million per person. For economy.

$1.5 million per person round-trip to SFO sounds crazy, but its a surprisingly appropriate analogy. Space shuttle missions cost over $200 million per astronaut. What if they could reuse the rocket?

SpaceX founder and CEO Elon Musks projection is that reusable rockets could bring the cost of space travel down 100x. But the implications are way bigger than making it cheaper for communications companies to fling satellites into low earth orbit.

Weve just gotten a step closer to becoming a multiplanetary species.

Right now, theres lots of talk of getting to Mars. Even Donald Trump has gotten in on the action, signing an order for a human mission to Mars by 2033.

Going to Mars is one thing. Weve sent people to the moon; we will eventually send people to Mars. But staying on Mars is something else entirely. In order to stay there, we need to send lots of people. We need ways to get them back if necessary. In other words, we have to make the round-trip flight between planets cost closer to $350 than $1.5 million.

And why would we want to stay there? Simple: to enhance the chances of humanitys survival.

Right now, we live at risk of a single event wiping out all of humanity. Solar flares, supervolcanoes In A Short History Of Nearly Everything, Bill Bryson describes two recent near-misses, when an asteroid passed within just 100,000 miles of earth. In cosmic terms, he said, this was the equivalent of a bullet passing through your sleeve without touching your arm.

Keeping the entire human species on one planet is a recipe for extinction.

And so the push to colonize other planets. A sustainable population on Mars means we double our chances of not being on the planet that gets hit by an asteroid.

But going to Mars also gives us a chance to create a new kind of society, without the baggage weve built up on earth.

On Mars, we could apply a new model of government. We could invent a new economic system. We could upend the legacy systems -- the historic racism, the systemic inequality, the ingrained poverty -- that make life on earth so painful for so many.

Idealistic fantasies? Sure. But possible. Right now, there are lots of people on earth trying to create systems that are fairer and more just, but they continually run up against the brick wall of civilizations inertia. The vacuum of space, however, has no such wall.

Time to get off this rock, methinks.

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The Colonization Of Mars - MediaPost Communications

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Stem cells help explain varied genetics behind rare neurologic disease – Science Daily

Posted: at 7:35 am

Stem cells help explain varied genetics behind rare neurologic disease
Science Daily
In the new study, published in The American Journal of Human Genetics, researchers used stem cells in their laboratory to simultaneously model different genetic scenarios that underlie neurologic disease. They identified individual and shared defects ...

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Stem cells help explain varied genetics behind rare neurologic disease - Science Daily

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DNA testing ordered on evidence from 2007 slaying; convicted man says he’s innocent – Omaha World-Herald

Posted: at 7:35 am

The forensic evidence recovered by Omaha police four bullet casings and a black T-shirt seemed almost an afterthought in a daylight shooting witnessed by multiple people.

Witnesses took the stand and put a killer away for life. And for 10 years, the items sat in an evidence locker, untested.

But thats about to change.

Douglas County District Judge Peter Bataillon recently ordered DNA examination on the casings and shirt in response to a motion filed late last year by Antoine D. Young. The 43-year-old Omaha man is eight years into a life term for the first-degree murder of Raymond Webb, shot repeatedly while behind the wheel of his car in an Omaha fast-food drive-thru.

Young has always insisted the witnesses got it wrong that he didnt kill anyone.

If forensic analysts can pull a genetic profile from the clothing and casings, it could help settle lingering questions about whether the right man is behind bars.

Douglas County Attorney Don Kleine agreed to allow the DNA tests without an appeal. But that doesnt mean the prosecutor now harbors doubts about Youngs guilt.

Were confident in our conviction, and we dont think theres any issue, Kleine said last week. So why not clear the air?

The judges decision also is significant because it marked the third time Young had sought DNA testing of the evidence.

Its definitely a huge step toward an exoneration for an innocent person, said Tracy Hightower-Henne, an Omaha lawyer with the Nebraska Innocence Project. Young also is being represented by two lawyers with the Midwest Innocence Project in Kansas City, Missouri.

The judges previous denials of Youngs motions for testing relied on a provision of state law that required defendants to show DNA testing was unavailable at the time of their original trials.

DNA testing was common by 2009, when a jury convicted Young, but his trial attorney didnt ask for it. Despite the fact that the evidence had never been tested, the courts essentially ruled that Young had blown his opportunity and was no longer entitled to the testing.

The Nebraska Supreme Court affirmed the rulings.

But in 2015 the Nebraska Legislature changed the DNA Testing Act, which had gone on the books in 2001.

The amended law makes it easier for Young and others by allowing courts to order the testing if biological evidence from the cases had not been analyzed before. Lawmakers did away with the need to show that the trials predated the advent of DNA technology.

The amended law also allows retesting of previously tested evidence if a defendant can show that new DNA technology could produce more accurate results.

The Innocence Project reports that of the 349 DNA exonerations in the United States since 1989, 71 percent involved eyewitness misidentification. Youngs trial turned on competing eyewitness testimony.

Jurors heard from six people who witnessed the shooting, which took place shortly before 3 p.m. on Aug. 25, 2007, as Webb sat in the drive-thru lane of a Taco Bell near 62nd Street and Ames Avenue. Four said the shooter wore a black shirt, one said the shirt was white and the sixth was unsure.

Two of the witnesses identified Young as the shooter, saying they were with Young outside a nearby barbershop when Webbs car pulled into the Taco Bell. The two witnesses said they saw Young run across the street and fire the gun through the drivers-side window of Webbs car.

Young now says he has evidence to prove one of those witnesses contacted Youngs brother before the murder trial and offered to withhold his testimony in exchange for $10,000. That witness is now dead.

At trial, three witnesses testified that Young was at a family picnic at a city park 4miles from the Taco Bell the entire afternoon of the shooting. Young has told the court he can produce additional witnesses who will vouch for his alibi.

In addition, Young asserts he can present yet a different witness who identified the shooter as another Omaha man who was released from prison last year after serving time for an unrelated gun crime.

Last summer, before the man was released from state custody, Youngs lawyers put him on the witness stand and asked if he killed Webb. The man invoked his constitutional right to not answer potentially incriminating questions.

The World-Herald is not naming the man because he has not been charged in connection with the case.

Records indicate that police were told there had been a long-running violent feud between the man and the victim. But they also show that authorities eliminated him as a suspect after he passed a polygraph examination.

In the recent DNA order, the judge said testing should be done on the evidence recovered from the crime scene as well as swabs taken from Young and the man whom Young accuses.

It remains to be seen if any identifiable DNA can be recovered from the evidence. Obtaining DNA from bullet casings has proven more difficult than from other sources.

The testing will be done at the University of Nebraska Medical Center and will be paid for by the Innocence Project.

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Pr. George’s police investigating DNA lab operations, suspended employee – Washington Post

Posted: at 7:35 am

Prince Georges County police suspended an employee and are conducting a review of the departments DNA lab after learning that the employee accredited work at a Texas facility that later had to be shut down.

The ongoing county review uncovered neglected DNA profiles that should have been entered into a national database, lags in notifying investigators of DNA profile matches and the use of outdated methods to calculate the individuality of profiles. As of late last week, the lapses identified did not appear to have affected any prosecutions or convictions, county Police Chief Hank Stawinski said.

Stawinski ordered the review of county lab operations in November and said the department suspended an employee within a day of launching the probe. The county employee also serves as a national accreditor in lab audits. Stawinski said he has alerted six other law enforcement agencies audited by the employee to scrutinize their lab operations.

We realized we had an issue and we took action in the county, Stawinski said. At this point, we have no instance where these administrative failings have led to a place where we could have prevented violent crime from occurring, and we dont have anyone innocent locked up in jail.

Police would not name the suspended lab employee, citing Maryland state personnel law. Individuals familiar with the inquiry identified her as Lynnett Redhead, who has been in charge of the DNA lab since 2007. Her attorney confirmed she is on paid administrative leave.

My client maintains that she did everything that was appropriate, up to the standard of care and up to the industry standards, said James Ellison, Redheads attorney.

Ellison said he and Redhead have not been told by the police department what the departments specific concerns are and could not respond to them directly.

Police said the main administrative problems they found after bringing in state and FBI officials so far affect 19 of about 4,200 cases. The lab remains in operation after outside inspections.

[What CSI and NCIS dont show you about the lives of crime-scene investigators]

In the course of its inquiry, the department also found unprocessed evidence that might generate new DNA profiles and benefit homicide cases dating to 2005, Stawinski said.

The comprehensive review comes as a national commission on forensic science grapples with quality assurance and with court testimony in the wake of DNA exonerations and police lab closures.

The shutdown of the Austin Police Departments DNA lab last year prompted the investigation in Prince Georges after the county learned its employee gave the Austin lab passing marks while serving as auditor for the American Society of Crime Laboratory Directors/Laboratory Accreditation Board, Stawinski said.

A state accrediting body for Texas later discovered employees in Austin were using outdated statistical and scientific methods to analyze DNA, had contaminated evidence and lacked proper training, according to local news reports. Austin police closed the lab in June.

[Austin crime lab scandal could affect more than 2,000 cases]

Ellison said Redhead was not the only person involved in accrediting the lab in Austin.

Its a process that involves multiple people, Ellison said of his client. She was very minimal in that accreditation process.

Officials with the accreditation board were not available for comment Friday, according to a woman who answered the organizations phone.

During the first 150 days of the internal investigation in Prince Georges, the department has found three primary concerns.

[Has DNA met its match as a forensic tool?]

DNA profiles collected in at least four homicide cases werent entered into a national database designed to identify links to other investigations.

In other instances, DNA profiles were entered into the national database and generated potential suspects in 12 burglary, sexual assault and homicide cases. But there was a lag in telling police investigators that a match had been spotted a problem that federal auditors had told the lab to fix in a 2010 audit. Instead of notifying investigations within the 30-day time frame that the federal government advises, the most recent tardy notifications discovered went out between several months to eight years after matches were flagged.

Analysts also have been found to be using outdated calculations to determine how likely it was that DNA collected from a scene could have come from someone other than a suspect. The outdated calculations were present in three cases that have gone through court, Stawinski said, all sexual assault cases that resulted in two convictions and one plea. In all three cases, the older calculations still generated statistically sound results, and using the new formula would not have changed the identifications, Stawinski said.

John Erzen, a spokesman for the Prince Georges County States Attorneys Office, said the police department alerted prosecutors of the investigation into the lab. As of Friday, prosecutors have not had to notify any defense attorney of new evidence that would be favorable to a defendant.

We have not received the results or the conclusions of the investigation, Erzen said. We have not had to notify anyone of anyone of any issues.

Its unclear when the review of the labs work will be complete, but Stawinski said the administrative concerns have been fixed.

We discovered these shortcomings because we chose to look for them, Stawinski said.

The chief also said the departments science is sound and stressed that the problems uncovered have been administrative. Im troubled that this occurred, but I promise the public and the community that these problems have been fixed and we will make sure they dont repeat themselves in the future.

[D.C. crime lab restarts DNA testing on limited basis after shutdown cast doubts over analysis]

The countys lab has been previously rebuked for at least one of the same administrative weaknesses occurring now.

In 2010, the U.S. Justice Department Office of the Inspector General released results of a routine audit that found the Prince Georges lab, while generally in compliance with industry standards, had some problems.

The audit report said the lab was storing DNA evidence in an unlocked freezer, leaving material susceptible to tampering, a practice that also had been called out by a 2008 audit.

The 2010 audit also found that 19 of 100 DNA profiles inspectors reviewed should not have been entered into the national database for various reasons. The lab also failed to confirm three DNA matches within the standard 30-day period and in three cases did not notify investigators of matches in a timely manner.

We believe that the Laboratorys delay in its confirmation of the matches are in violation of NDIS procedures, and we are concerned that its delay in notifying investigators in a timely manner could potentially lead to the suspected perpetrator committing additional crimes, the 2010 inspector generals report stated.

The problems flagged in the federal audit were fixed, according to the report and a letter from the county responding to the audit.

Lawrence Kobilinsky, professor of forensic science and science department chairman at John Jay College of Criminal Justice in New York, said that managing a lab that is efficient and thorough is just as important as ensuring analysts produce scientifically sound and accurate results.

If you dont do things in a timely way, you slow things down and you give people a chance to evade police detection, Kobilinsky said. Burglars especially. Theyre the king of recidivists. One may be the reason for 20 and 30 cases. You take one off the street, and all the sudden the burglary rate goes down.

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Pr. George's police investigating DNA lab operations, suspended employee - Washington Post

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Unavoidable typos in DNA help fuel cancer – Foster’s Daily Democrat

Posted: at 7:35 am

By Lauran NeergaardAP Medical Writer

WASHINGTON Cancer patients often wonder "why me?" Does their tumor run in the family? Did they try hard enough to avoid risks like smoking, too much sun or a bad diet?

Lifestyle and heredity get the most blame but new research suggests random chance plays a bigger role than people realize: Healthy cells naturally make mistakes when they multiply, unavoidable typos in DNA that can leave new cells carrying cancer-prone genetic mutations.

How big? About two-thirds of the mutations that occur in various forms of cancer are due to those random copying errors, researchers at Johns Hopkins University reported last month in the journal Science.

Whoa: That doesn't mean most cases of cancer are due solely to "bad luck." It takes multiple mutations to turn cells into tumors and a lot of cancer is preventable, the Hopkins team stressed, if people take proven protective steps.

Last month's report is an estimate, based on a math model, that is sure to be hotly debated by scientists who say those unavoidable mistakes of nature play a much smaller role.

But whatever the ultimate number, the research offers a peek at how cancer may begin.

And it should help with the "why me" question from people who have "done everything we know can be done to prevent cancer but they still get it," said Hopkins' Dr. Bert Vogelstein, a pioneer in cancer genetics who co-authored the study. "They need to understand that these cancers would have occurred no matter what they did."

What causes the mutations?

You might inherit some mutations, like flaws in BRCA genes that are infamous for causing aggressive breast and ovarian cancers in certain families.

More commonly, damage is caused by what scientists call environmental factors the assault on DNA from the world around us and how we live our lives. There's a long list of risks: Cigarette smoke, UV light from the sun, other forms of radiation, certain hormones or viruses, an unhealthy diet, obesity and lack of exercise.

Then there are those random copy errors in cells what Vogelstein calls our baseline rate of genetic mutations that will occur no matter how healthy we live.

One way to think of it: If we all have some mutations lurking in our cells anyway, that's yet another reason to avoid known risks that could push us over the edge.

How cells make typos

New cells are formed when an existing cell divides and copies its DNA, one cell turning into two. Every time DNA is copied, about three random mutations occur, Vogelstein said.

We all harbor these kinds of mutations and most don't hurt us because they're in genes that have nothing to do with cancer or the body's defense mechanisms spot and fix the damage, said Dr. Otis Brawley of the American Cancer Society, who wasn't involved in the new research.

But sometimes the errors hit the wrong spot and damage genes that can spur cancerous growth or genes that help the cell spot and fix problems. Then the damaged cells can survive to copy themselves, allowing important mutations to gradually build up over time. That's one reason the risk of cancer increases with age.

The study findings

Thursday's study follows 2015 research by Vogelstein and statistician Cristian Tomasetti that introduced the idea that a lot of cancer may be due to "bad luck," because those random DNA copying mistakes are more common in some kinds of cancer than others. Cancer prevention advocates worried the idea might sway people to give up on healthier lifestyles.

This time around, the duo analyzed mutations involved in 32 types of cancer to estimate that 66 percent of the gene flaws are due to random copy errors. Environmental and lifestyle factors account for another 29 percent, while inherited genes made up just 5 percent of the mutations.

Different organs, different risks

The same person can harbor a mix of mutations sparked by random DNA mistakes, heredity or environmental factors. And which is the most common factor differs by cancer, the Hopkins team said.

For example, team members estimate that random cell errors account for 77 percent of critical mutations in pancreatic cancer while still finding some caused by lifestyle risks like smoking. And the random DNA mistakes caused nearly all the mutations leading to childhood cancers, which is not surprising because youngsters have had little time to be exposed to environmental risks.

In contrast, most lung cancer mutations were the result of lifestyle factors, mainly from smoking. And while lung tissue doesn't multiply frequently, the small number of mutations caused by chance DNA errors might explain rare cases of never-smokers who still get sick.

"This paper is a good paper," said the cancer society's Brawley. "It gives prevention its due respect."

Other scientists see more to the story

Estimates from Britain suggest 42 percent of cancers are potentially preventable with a healthy lifestyle, and the Hopkins team says their mutation research backs that idea.

But Dr. Yusuf Hannun, Stony Brook University's cancer center director, contends that's just the number known to be preventable today researchers may discover additional environmental risks we can guard against in the future.

He said the Hopkins paper exaggerates the effect of the unavoidable DNA mistakes. His own 2015 research concluded they account for 10 percent to 30 percent of cancer cases.

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Fiat DNA is thriving in Japanese-built 124 Spider – Press Herald

Posted: at 7:35 am

A lady. A lovely, sensual, responsive Italian lady.

Thats how Car and Driver magazine described the Fiat 124 after its debut in the 1960s.

The sentiments no doubt sexist by todays standards, but heck, it was the 60s. And there was a point to the seductive words: Not all sports cars have to be hard-edged.

This one had all the mechanicals and quickness and a sporty suspension but also a more refined interior and quieter ride.

After a nearly 40-year absence (the classic endured till 1978, then buzzed around till 85 as the Fiat 2000), the Fiat 124 Spider has returned in the form of a 2017 model. But this time the Italian lady has a factory live-in friend, the Mazda Miata.

Mazda, you see, manufactures the 124 alongside the Miata at its Hiroshima, Japan, plant and provides many of its underpinnings.

But the 124 (base price: $27,495; as tested, $33,635) retains the shape and body creases of its legendary Pininfarina design, and its engine is actually prebuilt and shipped from a Fiat plant in Italy. So its Fiat DNA is alive and well.

It also is 5 inches longer, has more upscale materials inside and boasts a noticeably quieter ride than the Miata. Acoustic glass and headliner help keep noise to a conversation-friendly level.

In contrast to Mazdas naturally aspirated 2.0-liter engine, the Spider gets a turbocharged 1.4-liter engine that puts out 160 horsepower and 184 pound-feet of torque (4 more horses ride along with the Abarth version).

Shifting the standard manual tranny in this little bug-eyed roadster is a blast thanks to its tight six-speed shift box. A six-speed automatic is optional on all trims and, while it ticks smoothly, it can be a little sluggish to respond.

On the road, the rear-wheel-drive 124 is quick and nimble. Corners are taken confidently thanks to precision steering and Fiats own suspension components. Bumps and dips are gobbled up around town, too.

The highway ride is comfortable for a roadster, and an EPA-estimated 36 mpg makes it even more tempting to take out on a road trip (25 mpg around town).

Flipping down the soft top, which has a glass rear window, is easy-breezy. It takes less than a minute and can be done without leaving the drivers seat. Just unlatch at the top and toss it behind you.

Put the top up and taller folks will find the Spiders cabin a little cramped. Roadsters typically are like airplane cockpits for those over 6 feet tall: They must carefully squeeze in each of the body parts. Same deal here, with limited headroom, legroom and elbow room.

Likewise, weekend jaunts will require thoughtful packing with a mere 4.9 cubic feet of trunk space available.

But the interior has a nice look and feel, with soft-touch materials surrounding the instrument panel and leather-wrapped steering wheel. There are piano-black accents throughout. A 7-inch touchscreen (optional on the base model) is bright and clear, and its menus easy to navigate. A nine-speaker Bose sound system is available.

Three trim levels are offered. It starts with the base Classica, which gets 16-inch alloys, push-button start and a tiny 3-inch touchscreen. The Lusso adds 17-wheel wheels, fog lights, leather seats and a tech package with rear-view camera and bigger touchscreen.

For the racing-inspired, theres the top-line Abarth: quad exhaust tips, limited-slip rear differential, adjustable driving modes and Brembo high-performance brakes, plus some sporty interior accents like simulated suede seats.

Fiat even includes racing-school instruction in Arizona to every Abarth buyer willing to make the trip.

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Could Genome Sequencing Save Mauritius’ Pink Pigeon? – Island Conservation News

Posted: at 7:34 am

The Pink Pigeon (Nesoenas mayeri) is an Endangered species on the Island of Mauritius in the Indian Ocean. The existence of the species is somewhat of a miracle consideringthat in the 1990s its population fell to an alarming 10 individuals. Now the population hovers around 400, but the birds still face a number of threats.

While the sudden growth in population seems like a good thing, it had an unintended consequence. Increasing the population at such a rapid rate from only 10 individuals resulted in a population with low genetic variation. Low genetic variation can make species more vulnerable to threats.

The population faces threats of invasive species and a human introduced pathogen called Trichimonas gallinaeleaves, which is toxic to approximately 60% of Pink Pigeon offspring. The portion of the population that is not harmed appears to have some immunity to the pathogen.

Pink Pigeons are a vital part of the Mauritius ecosystem. Credit: Sergey Yeliseev

Researchers at the Earlham institute and the University of East Anglia want the Pink Pigeon to be the first endangered bird species to have its genome sequenced. The belief is that by sequencing their genes, they can identify immune system genes that could protect them against the pathogen. The head of the campaign to sequence the Pink Pigeons genes commented:

Halting species extinction may be possible when the main cause of extinction has a genetic basis, particularly when genetic variation needed to supplement and rescue the species is still available in either the captive or wild populations. Our plan uses the pink pigeon to show how this can be achieved, creating a framework that could be easily transferred to other species across the world.

Sequencing the Pink Pigeons genome would give researchers more information to save the species from extinction. Pink Pigeons are an important part of the Mauritius island ecosystemhopefully new research will help them thrive once again.

Featured photo: Pink Pigeon perching. Credit: Josh Noseworthy Source: Cambridge Network

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Could Genome Sequencing Save Mauritius' Pink Pigeon? - Island Conservation News

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Psoriasis care goal is control with least toxic treatment – Altoona Mirror

Posted: at 7:32 am

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Apr 2, 2017

Dear Dr. Roach: I have psoriasis. I have used clobetasol for 22 years. The psoriasis is not severe, but its constant.

Should I be concerned about using this treatment for so many years? The only time it cleared up (and that was for two years) was when I had to take steroids for poison ivy.

The doctor would not put me on a low dose of steroid to see if the psoriasis would stop completely and will not use other treatments, because both my brother and sister died of cancer.

Any suggestions? I had two co-workers with psoriasis that was much worse than mine, and for some reason it disappeared for both of them after 20 years.

R.M.

Answer: For mild to moderate psoriasis, a skin disorder that most commonly manifests with scaly plaques, the goal of care is to control symptoms using the least toxic therapies available. That means topical therapies, like clobetasol cream or ointment, and other treatments for instance, vitamin D-like or vitamin A-like drugs.

These are very safe to use long-term for most people, if used correctly under supervision (clobetasol, a powerful steroid, used in the wrong place, especially the face, can cause permanent atrophy).

If you have had good response to these, they are your best choice. However, it sounds like you havent had as good a response as you want.

I am curious about your response to the oral steroids you took for poison ivy. Normally, we treat moderate to severe poison ivy with a week or so of oral steroids.

If just that much gave you two years of freedom from psoriasis, then I dont understand why your doctor cant give you a short course of steroids on a very-infrequent basis.

For severe psoriasis, systemic treatments are essential; however, they do have risks. Steroids are not a usual systemic treatment for psoriasis.

Methotrexate, a drug used for cancer and in serious autoimmune diseases, is well-studied and tolerated by most.

Vitamin A relatives, like acitretin (Soriatane), are very effective. Biological therapies, like etanercept (Enbrel), also have a clear place in treating severe psoriasis, but all of these drugs have potential for harm, including an increased risk of certain types of cancer.

In your case, I would consider getting a second opinion from a dermatologist with expertise in psoriasis.

If the advice is the same, you can feel confident in the advice; if not, you will need to decide which course to follow.

Dear Dr. Roach: You recently had a column where you did not recommend alprazolam (Xanax) as a long-term sleep aid. What are the negative effects of using it that way? A.T.

Answer: Alprazolam is in the class of drugs called benzodiazepines, which includes Valium, Klonopin and Halcyon.

They are effective at getting people to sleep more quickly, and increase total sleep time by 30-60 minutes.

Alprazolam is very short-acting (although there is a long-acting form now) and is not indicated for insomnia.

I dont recommend benzodiazepines because they increase the rate of falls, especially in the elderly, because they can cause memory loss and because they can cause confusion and dependence.

I try to avoid prescribing sleeping medications, and most people with occasional difficulty sleeping do well with sleep hygiene advice: Having a regular sleep schedule, not trying to force sleep, avoiding alcohol and caffeine near bedtime and not using bright lights or computer screens before bed are part of this.

If I do prescribe a sleep medication, I recommend using it no more than every other day and for no more than two weeks. People who need more than that, I refer to a sleep specialist.

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Psoriasis care goal is control with least toxic treatment - Altoona Mirror

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