Daily Archives: September 12, 2014

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By: Gerald Soh

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By Jim Newton Sun-Times Media September 11, 2014 5:38PM

Marni Yang (left) was found guilty of gunning down Rhoni Reuter (right) in her Deerfield condo in 2007. | File

storyidforme: 71820830 tmspicid: 3183981 fileheaderid: 1842383

In prison for killing Rhoni Reuter, the pregnant girlfriend of ex-Chicago Bears player Shaun Gayle, Marni Yang is hoping new DNA tests will exonerate her.

On Thursday, Lake County Circuit Court Judge Christopher Stride signed an order for the testing of shell casings from the bullets used to kill Reuter, 42, of Deerfield, who was seven months pregnant when she was killed in 2007.

I know her trial was highly watched, and the evidence seemed overwhelming, said Jed Stone, Yangs defense attorney. But we are on a truth-seeking mission.

Stone said he has retained a highly respected DNA expert to attempt to extract touch DNA from the shell casings, which Stone said could identify the real killer.

Assistant States Attorney Ari Fisz said prosecutors have no objection to Stones request for new DNA testing.

After she was convicted in Lake County Circuit Court, Yang lost an appeal in the 2nd District Appellate Court, and the Illinois Supreme Court refused to hear her case.

But Stone said Yangs first, direct appeal was limited to information used during the trial. Stone is now putting together a post-conviction petition that can bring in new evidence that wasnt heard at trial.

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New DNA tests ordered in murder of Shaun Gayles girlfriend

Researchers from North Carolina State University, Duke University and the University of Copenhagen have created the world's largest DNA origami, which are nanoscale constructions with applications ranging from biomedical research to nanoelectronics.

"These origami can be customized for use in everything from studying cell behavior to creating templates for the nanofabrication of electronic components," says Dr. Thom LaBean, an associate professor of materials science and engineering at NC State and senior author of a paper describing the work.

DNA origami are self-assembling biochemical structures that are made up of two types of DNA. To make DNA origami, researchers begin with a biologically derived strand of DNA called the scaffold strand. The researchers then design customized synthetic strands of DNA, called staple strands. Each staple strand is made up of a specific sequence of bases (adenine, cytosine, thaline and guanine -- the building blocks of DNA), which is designed to pair with specific subsequences on the scaffold strand.

The staple strands are introduced into a solution containing the scaffold strand, and the solution is then heated and cooled. During this process, each staple strand attaches to specific sections of the scaffold strand, pulling those sections together and folding the scaffold strand into a specific shape.

The standard for DNA origami has long been limited to a scaffold strand that is made up of 7,249 bases, creating structures that measure roughly 70 nanometers (nm) by 90 nm, though the shapes may vary.

However, the research team led by LaBean has now created DNA origami consisting of 51,466 bases, measuring approximately 200 nm by 300 nm.

"We had to do two things to make this viable," says Dr. Alexandria Marchi, lead author of the paper and a postdoctoral researcher at Duke. "First we had to develop a custom scaffold strand that contained 51 kilobases. We did that with the help of molecular biologist Stanley Brown at the University of Copenhagen.

"Second, in order to make this economically feasible, we had to find a cost-effective way of synthesizing staple strands -- because we went from needing 220 staple strands to needing more than 1,600," Marchi says.

The researchers did this by using what is essentially a converted inkjet printer to synthesize DNA directly onto a plastic chip.

"The technique we used not only creates large DNA origami, but has a fairly uniform output," LaBean says. "More than 90 percent of the origami are self-assembling properly."

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World's largest DNA origami created