Monthly Archives: January 2013

DNA, ball cap tie suspect to robbery

Posted: January 8, 2013 at 8:52 pm

The attorney for a man accused of robbing a service station/convenience store in Redstone Township in 2007 says his client was out of state when the crime occurred; prosecutors say DNA puts him at the scene.

That was the case Monday before Fayette County Judge John F. Wagner Jr., with defense testimony expected to be heard today.

Vikram Yamba, 28, of Monroeville is charged with aggravated assault, robbery, illegal possession of a firearm, simple assault, recklessly endangering another person and theft in connection with the April 18, 2007, crime.

Neither defense attorney David Kaiser nor prosecutor Doug Sepic are disputing the commission of the crime: that two men entered the Citgo station on Route 40 around 5 a.m. April 18, 2007, and that a man wearing a yellow Pittsburgh Pirates ball cap fired two shots from a handgun after demanding money from the clerk, who refused to comply.

Videotape from the store surveillance system was shown in court Monday, showing the two men entering the store, one wearing a green hat, the other a yellow one. Two hats matching the descriptions were found down the road from the business, near the intersection of Route 166 and Simpson Road, along with a striped sweatshirt, a T-shirt and a long-sleeved black T-shirt, clothing items seen on the suspects in the video.

The store clerk, Diane Hatfield, identified the long-sleeved T-shirt as the item the man with the gun and the yellow cap used to cover his face.

Two experts from the state police laboratory in Greensburg testified that DNA samples were taken from the sweatbands on the ball caps. The DNA sample from the green cap has not yielded any matches, but in 2011 the sample from the yellow cap, known as Item 5 in the police inventory, was positively matched to DNA from Yamba, leading to his arrest in the case.

Forensic scientist Julia Brolley testified that only one persons DNA was found on the band from Item 5 and it was matched to a known sample of DNA taken from Yamba.

Kaiser said a robbery occurred, but it wasnt committed by his client.

He was actually in Maryland at the time of the incident. The only connection is the DNA found on a hat a couple hundred yards away, Kaiser said. No one identified him.

See more here:
DNA, ball cap tie suspect to robbery

Posted in DNA | Comments Off on DNA, ball cap tie suspect to robbery

Alliance man on death row pins hopes on DNA test

Posted: at 8:52 pm

The attorney for an Alliance man on death row for a double murder says a new DNA test on a cigarette butt could be a key part of evidence if her client got a new trial.

The attorney representing the state says new analysis would not prove Tyrone Noling is not guilty even if the cigarette butt was linked to a man who had once been a suspect in the case.

The Ohio Supreme Court heard those arguments Tuesday in Columbus and took Nolings request for new DNA testing under advisement.

Noling was convicted of shooting and killing an elderly couple, Bernhardt and Cora Hartig, in their Atwater home in Portage County in 1990. He was sentenced to death in 1996.

The cigarette butt was found in the driveway of the Hartigs home and DNA showed it did not match Noling.

Carrie Wood, an attorney with the Ohio Innocence Project, said she wants to determine if the cigarette butt contains the DNA of Daniel Wilson.

Wilson, convicted of murder in another case, was executed in 2009. He lived near the elderly couples home.

Both attorneys argued over whether state law allows more DNA testing in the case. Nolings request was denied previously based on state law at the time.

State law allows for more DNA testing, not less, Wood said.

Victor V. Vigluicci, the attorney representing the state, said that even if a new test matches Wilsons DNA to the cigarette butt, it doesnt prove he killed the elderly couple. At most it would indicate Wilson flicked a cigarette butt out the window while driving by the home, Vigluicci said.

Go here to see the original:
Alliance man on death row pins hopes on DNA test

Posted in DNA | Comments Off on Alliance man on death row pins hopes on DNA test

DNA provides break in UNC student's murder

Posted: at 8:52 pm

CHAPEL HILL -- DNA evidence left at the scene of the murder of a UNC-Chapel Hill student last September has provided a break in the case, Chapel Hill police announced Tuesday morning.

The body of Faith Danielle Hedgepeth was found at about 11 a.m. Sept. 7, 2012, by her roommate in the apartment they shared at 5639 Old Chapel Hill Road (Hawthorne at the View apartment complex).

The investigation has revealed that Hedgepeth and her roommate went to a local Chapel Hill nightclub, The Thrill, early that morning. She was last known to be alive around 3 a.m. at the apartment.

The Chapel Hill Police Department investigators have partnered with other local, state and federal agencies on the case. Evidence analyzed by the N.C. State Crime Lab has revealed DNA left at the scene by a male suspect, according to police.

Investigators are confident this DNA will identify the offender and help us to exclude individuals not involved in this crime, police said in a news release.

After consulting with the Federal Bureau of Investigations Behavioral Analysis Unit, police now believe identifiers of the offender may include:

The suspect was familiar with Hedgepeth and may have lived near her in the past.

The suspect was unaccounted for during the early morning hours of Sept. 7, 2012.

The suspect may have made comments regarding Hedgepeth to close associates in the past.

There may have been some change in the suspects behavior after the murder (including an unusual interest in the case) or a change in his performance at work or school.

Original post:
DNA provides break in UNC student's murder

Posted in DNA | Comments Off on DNA provides break in UNC student's murder

DNA prefers to dive head first into nanopores

Posted: at 8:52 pm

Jan. 8, 2013 If you want to understand a novel, it helps to start from the beginning rather than trying to pick up the plot from somewhere in the middle. The same goes for analyzing a strand of DNA. The best way to make sense of it is to look at it head to tail.

Luckily, according to a new study by physicists at Brown University, DNA molecules have a convenient tendency to cooperate.

The research, published in the journal Physical Review Letters, looks at the dynamics of how DNA molecules are captured by solid-state nanopores, tiny holes that soon may help sequence DNA at lightning speed. The study found that when a DNA strand is captured and pulled through a nanopore, it's much more likely to start the journey at one of its ends, rather than being grabbed somewhere in the middle and pulled through in a folded configuration.

"We think this is an important advance for understanding how DNA molecules interact with these nanopores," said Derek Stein, assistant professor of physics at Brown, who performed the research with graduate students Mirna Mihovilivic and Nick Haggerty. "If you want to do sequencing or some other analysis, you want the molecule going through the pore head to tail."

"What we found was that ends are special places ... and that has a consequence for the likelihood a molecule starts its journey from the end."Research into DNA sequencing with nanopores started a little over 15 years ago. The concept is fairly simple. A little hole, a few billionths of a meter across, is poked in a barrier separating two pools of salt water. An electric current is applied across the hole, which occasionally attracts a DNA molecule floating in the water. When that happens, the molecule is whipped through the pore in a fraction of a second. Scientists can then use sensors on the pore or other means to identify nucleotide bases, the building blocks of the genetic code.

The technology is advancing quickly, and the first nanopore sequencing devices are expected to be on the market very soon. But there are still basic questions about how molecules behave at the moment they're captured and before.

"What the molecules were doing before they're captured was a mystery and a matter of speculation," Stein said. "And we'd like to know because if you're trying to engineer something to control that molecule -- to get it to do what you want it to do -- you need to know what it's up to."

To find out what those molecules are up to, the researchers carefully tracked over 1,000 instances of a molecule zipping through a nanopore. The electric current through the pore provides a signal of how the molecule went through. Molecules that go through middle first have to be folded over in order to pass. That folded configuration takes up more space in the pore and blocks more of the current. So by looking at differences in the current, Stein and his team could count how many molecules went through head first and how many started somewhere in the middle.

The study found that molecules are several times more likely to be captured at or very near an end than at any other single point along the molecule.

"What we found was that ends are special places," Stein said. "The middle is different from an end, and that has a consequence for the likelihood a molecule starts its journey from the end or the middle."

More:
DNA prefers to dive head first into nanopores

Posted in DNA | Comments Off on DNA prefers to dive head first into nanopores

Hacking the Human Genome Using Clojure and Similarity Search – Arnoldo Jose Muller-Molina – Video

Posted: at 8:51 pm


Hacking the Human Genome Using Clojure and Similarity Search - Arnoldo Jose Muller-Molina
The Genome inside each cell works like a massively parallel computer. Some proteins called Transcription Factors (TF) attach into specific regions called "promoters". This attachment starts a complex process that can have different outcomes. One of the possible outcomes is the creation of another TF that will in turn attach to some promoter(s) creating a cascade of events. TFs are like functions that have side effects, call other TFs and also can call themselves recursively. In this talk, I will describe a machine learning technique that attempts to reverse engineer the Genome. To achieve this tricky task, you need versatile tools. First of all, Clojure plays an instrumental role in the development of visualizations and data processing pipelines. Clojure makes it really easy to filter, visualize, and synthesize many gigabytes of data. In addition, similarity search is used extensively to find patterns in a huge set of possibilities. I hope to convince you here that similarity search is the next "NoSQL" and that Clojure is an ideal tool for data science projects.

By: ClojureTV

Continue reading here:
Hacking the Human Genome Using Clojure and Similarity Search - Arnoldo Jose Muller-Molina - Video

Posted in Genome | Comments Off on Hacking the Human Genome Using Clojure and Similarity Search – Arnoldo Jose Muller-Molina – Video

Genome MIDI Sequencer / Animoog iPad problem – Video

Posted: at 8:51 pm


Genome MIDI Sequencer / Animoog iPad problem
I set everything up according to all the manuals an online tutorial videos available. However, when I drew the notes into the Genome pattern the sound was 100% different from the sound that was coming from Animoog when I played directly on its keyboard (the sound is a continuous bass, and when triggered by Genome it sounded like a bass drum - short spiky sound). Plus, out of many notes that were present in the pattern only the first one was triggered, the rest were silent. After trying to find what the problem was I found out that the sound was played as it supposed to only when I would turn on the key hold button in Animoog. I am completely clueless on what it is caused by.

By: Ivan Kuptsov

Read more from the original source:
Genome MIDI Sequencer / Animoog iPad problem - Video

Posted in Genome | Comments Off on Genome MIDI Sequencer / Animoog iPad problem – Video

SCIENCEEE! – Episode 1 – Bacon: The Genome – Video

Posted: at 8:51 pm


SCIENCEEE! - Episode 1 - Bacon: The Genome
On this brand new show David, Brett, and Andy attempt to boost your IQ with some bacon talk with Kyle Schachtschneider about his previous research project about bacon (a link to his intense but awesome research project). He mapped out porcine genome, and plans to continue to rock the world with his SCIENCEEE!! Stay tuned for future episodes of this show where we poke and prod at the world of science in order to grow, learn, and get smarts.

By: GeekifyEntertainment

Read more here:
SCIENCEEE! - Episode 1 - Bacon: The Genome - Video

Posted in Genome | Comments Off on SCIENCEEE! – Episode 1 – Bacon: The Genome – Video

100K Pathogen Genome Project Selects PacBio SMRT(R) DNA Sequencing to Generate High-Quality, Finished Genomes

Posted: at 8:51 pm

MENLO PARK, Calif., Jan. 8, 2013 (GLOBE NEWSWIRE) -- Pacific Biosciences of California, Inc. (PACB) provider of the PacBio(R)RS High Resolution Genetic Analyzer, and the University of California, Davis (UC Davis) today announced a partnership for the 100K Pathogen Genome Project. As part of the project, Pacific Biosciences' Single Molecule, Real-Time (SMRT(R)) technology will be used to sequence the genomes from at least 1,000 foodborne pathogen samples to completion, and to elucidate their epigenomes. These bacteria represent major illness-causing pathogens, including Salmonella, Campylobacter, E. coli, Vibrio, and Listeria.

The 100K Genome Project was founded by the U.S. Food & Drug Administration, Agilent Technologies, and the laboratory of Dr. Bart Weimer at UC Davis to create a consortium of partners from around the world that will sequence 100,000 foodborne pathogens using next-generation sequencing. This initiative addresses a significant shortage of bacterial pathogen information for use in designing molecular diagnostics, creates a resource to expand our understanding of infection mechanisms, and constructs a public repository for new insights into bacterial evolution by using large-scale genomics.

Pacific Biosciences' SMRT sequencing technology generates sequence reads an order of magnitude longer than other leading DNA sequencing technologies, thereby facilitating efficient de novo microbial genome assemblies. Long reads are critical for resolving genetic complexity in the assembly and finishing of genomes. The use of SMRT sequencing for the automated finishing of microbial genomes has been demonstrated in multiple recent publications, including for the genetic analysis of the Haitian cholera and German E. coli outbreaks.

The kinetic information acquired during SMRT sequencing can be used to elucidate the epigenome of bacteria. Epigenetic DNA base modifications, such as methylation, play an important role in the phenotypic variation, adaptability and pathogenicity of many bacteria, but they have been difficult to study due to the lack of a sequencing method to detect them. As part of the 100K Genome Project, the epigenomes of the pathogenic strains subjected to SMRT sequencing will be characterized, adding an important dataset to public database repositories.

"SMRT sequencing has been shown to be a powerful technology for the comprehensive determination of microbial genomes and epigenomes," said Dr. Jonas Korlach, Chief Scientific Officer of Pacific Biosciences. "Through the combination of long reads, high consensus accuracy, and the lack of sequencing bias to GC content or sequence contexts, SMRT sequencing harbors the necessary requirements to construct finished genomes in an unbiased, hypothesis-free manner. The ability to detect methylation as part of the sequencing process is unique to SMRT sequencing, and will provide an invaluable resource to illuminate the epigenetic components controlling bacterial pathogenicity."

"We are very pleased to utilize SMRT sequencing as part of the 100K Genome Project," said Bart Weimer, Professor and Director of the 100K Genome Project, "SMRT technology will enable production of complete genomes that will contribute great value toward databases for biological insight, new biomarker discovery, and reference genomes for food pathogen detection. A project of this scale is needed since microbial genome variations, including structural variations, the acquisition and loss of mobile elements, and phages or plasmids, are very difficult or impossible to detect without a de novo sequencing and genome assembly approach, yet they have a significant impact on food safety."

The partnership will entail the sequencing of at least 1,000 samples by the 100K consortium member labs with access to the PacBio RS instrumentation, including pipeline constructions for high-throughput pathogen sequencing, de novo genome assemblies, epigenome determination, and data curation and deposition. Pacific Biosciences will provide technical guidance and training to support these activities, and interface closely with the involved laboratories to assist in the efficient construction of these pipelines.

For more information, please visit http://100kgenome.vetmed.ucdavis.edu/index.cfm and http://www.pacb.com.

About Pacific Biosciences

Pacific Biosciences of California, Inc. (PACB) offers the PacBio(R)RS High Resolution Genetic Analyzer to help scientists solve genetically complex problems. Based on its novel Single Molecule, Real-Time (SMRT(R)) technology, the company's products enable: targeted sequencing to more comprehensively characterize genetic variations; de novo genome assembly to more fully identify, annotate and decipher genomic structures; and DNA base modification identification to help characterize epigenetic regulation and DNA damage. By providing access to information that was previously inaccessible, Pacific Biosciences enables scientists to increase their understanding of biological systems.

Go here to see the original:
100K Pathogen Genome Project Selects PacBio SMRT(R) DNA Sequencing to Generate High-Quality, Finished Genomes

Posted in Genome | Comments Off on 100K Pathogen Genome Project Selects PacBio SMRT(R) DNA Sequencing to Generate High-Quality, Finished Genomes

Wet Dressing for treatment of Eczema. – Video

Posted: at 8:51 pm


Wet Dressing for treatment of Eczema.
Welcome to Allerchic #39;s Instructional video on how to apply a wet dressing for the treatment of Eczema Allergy #39;s for more information including facts sheets please visit http://www.allerchic.com.au

By: Allerchic

Read the original here:
Wet Dressing for treatment of Eczema. - Video

Posted in Eczema | Comments Off on Wet Dressing for treatment of Eczema. – Video

PMG Psoriasis – Video

Posted: at 8:51 pm


PMG Psoriasis

By: TheKellyBerger

More here:
PMG Psoriasis - Video

Posted in Psoriasis | Comments Off on PMG Psoriasis – Video