Daily Archives: December 2, 2012

ND Tiwari dances during qawwali performance - NewsX
Former Uttar Pradesh chief minister and senior Congress leader ND Tiwari was caught on camera swaying to a qawwali in Barabanki. The 87-year-old was recently in news after a DNA report declared him the biological father of Delhi-based youth Rohit Shekhar in a paternity suit. Tiwari, three-time Chief Minister of Uttar Pradesh, also served as Uttarakhand Chief Minister between 2002 and 2007. He held the External Affairs portfolio between 1986-87 and was also the Governor of Andhra Pradesh between 2007 and 2009.From:newsxliveViews:29 0ratingsTime:00:38More inNews Politics

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ND Tiwari dances during qawwali performance - NewsX - Video

Dna Evol -Demented-
Dna Evol -the unholy warunions of hell goats- Raw Black Metal #39; Funeral Doom #39; Ambient #39; Harsh Noise http://www.facebook.comFrom:MrPtomainViews:0 0ratingsTime:00:56More inMusic

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A year after a Globe investigation found restaurants and stores across Massachusetts were routinely selling cheaper, lower-quality fish than they promised customers, a new round of DNA testing shows the vast majority are still mislabeling seafood.

Kens Steak House in Framingham again served Pacific cod instead of a more expensive Atlantic species. Slices of fish sold as white tuna at Sea To You Sushi in Brookline were again actually escolar, an oily species nicknamed the ex-lax fish by some in the industry because it can cause digestion problems. H Mart, an Asian supermarket chain found to have sold mislabeled red snapper last year, this time was selling inexpensive freshwater Nile perch as pricier ocean grouper at its Burlington store.

The results underscore an ongoing lack of regulation in the nations seafood trade oversight so weak restaurants and suppliers know they will not face punishment for mislabeling fish. Over the past several months, the Globe collected 76 seafood samples from 58 of the restaurants and markets that sold mislabeled fish last year. DNA testing on those samples found 76 percent of them werent what was advertised.

Some restaurant operators who repeatedly mislabeled fish blamed suppliers. Others said naming inconsistencies were the result of clerical errors. Several made only partial revisions to their menus. Some, like at Hearth n Kettle in Attleboro, corrected their menus, but waitstaff still wrongly described the fish as local. And a few said the issue was not a priority.

Were too busy to deal with such silliness, Janet Cooper, of Kens Steak House, said after several phone interviews during which she could not explain why the restaurant was still selling far less expensive Pacific cod as locally caught fish.

After the Globes Fishy Business series last fall, state and federal lawmakers pledged quick action to strengthen oversight of the seafood industry. US Representative Ed Markey, Democrat of Malden, filed a bill in July to require traceability of fish from the boat to the dinner plate, but the legislation hasnt moved out of House subcommittees.

Elsewhere, little progress has been made to protect consumers from paying too much for inferior fish. The Food and Drug Administration, which maintains a list of acceptable market names for fish species, has historically focused efforts on food safety, rather than economic fraud such as seafood substitution. The agency recently began conducting its own DNA testing, but the results so far have provided little insight into where mislabeling occurs in the supply chain.

The Globe hired the Biodiversity Institute of Ontario at the University of Guelph to conduct DNA testing on the fish samples, as it did for the initial round of tests in 2011. The testing focused on certain species, such as red snapper and cod, because they have been identified by regulators as more likely to be substituted.

Seafood mislabeling persisted at the Sand Bar & Grille on Marthas Vineyard, where the Globe last year found farmed hybrid bass was switched for striped bass, and tilapia was misrepresented as red snapper.

Mike Wallace, who runs the Oak Bluffs restaurant, said he talked with his sushi chef after the Globes initial investigation and believed the issue was resolved. But DNA testing this year showed samples of albacore and red snapper were both tilapia, one of the cheapest farmed fish on the market.

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New round of DNA tests finds dozens of repeat offenders in fish mislabeling

By Ed Yong | November 29, 2012 1:17 pm

For tens of thousands of years, humans have created sculptures by carving pieces from a solid block. They have chipped away at stone, metal, wood and ceramics, creating art by subtracting material. Now, a group of scientists from Harvard University have figured out how to do the same thing with DNA.

First, Yonggang Ke builds a solid block of DNA from individual Lego-like bricks. Each one is a single strand of the famous double helix that folds into a U-shape, designed to interlock with four neighbours. You can see what happens in the diagram below, which visualises the strands as two-hole Lego bricks. Together, hundreds of them can anneal into a solid block. And because each brick has a unique sequences, it only sticks to certain neighbours, and occupies a set position in the block.

This means that Ke can create different shapes by leaving out specific bricks from the full set, like a sculptor removing bits of stone from a block. Starting with a thousand-brick block, he carved out 102 different shapes, with complex features like cavities, tunnels, and embossed symbols. Each one is just 25 nanometres wide in any direction, roughly the size of the smallest viruses.

Kes work, led by Harvards Peng Yin, is the latest achievement from the growing field of DNA origami. Its forefather was the chemist Ned Seeman, who created a DNA cube in 1991 by annealing separate strands together. He followed this with a simple tubes and lattices, but his technique was laborious and inefficient.

Paul Rothemund greatly improved it in 2006. He showed that you can fold a long 7,000-letter strand of viral DNA into a specific shape by using hundreds of shorter snippets. These match different part of the viruss genome and staple it into place. Mix the strands togetherthe long scaffold and short staplesand they spontaneously fold into the right shape. Rothemund and others used the origami technique to create miniature maps, smiley faces, the word NED (in honour of Seeman), and more elaborate shapes like boxes.

Last year, Yins team broke off from the scaffold-and-staples tradition. I wrote about their work for Nature News:

Bryan Wei and his colleagues make shapes out of single strands of DNA just 42 letters long. Each strand is unique, and folds to form a rectangular tile. When mixed, neighbouring tiles stick to each other in a brick-wall pattern, and shorter boundary tiles lock the edges in place.

In their simplest configuration, the tiles produce a solid 64-by-103-nanometre rectangle, but Wei and his team can create more complex shapes by leaving out specific tiles. Using this strategy, they created 107 two-dimensional shapes, including letters, numbers, Chinese characters, geometric shapes and symbols. They also produced tubes and rectangles of different sizes, including one consisting of more than 1,000 tiles.

The team designed a robot to pick the tiles. The desired shape is drawn using a graphical interface, and the robot picks out and mixes the required strands. It can produce 48 shapes in as many hours. Millions of shapes can be crafted from the same set of tiles simply by leaving some out. Once you have a pre-synthesized library, you dont need any new DNA designs, says Yin. You just pick your molecules.

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DNA Lego bricks produce nano-sculptures

Fifty-nine years after James Watson and Francis Crick deduced the double-helix structure of DNA, a scientist has captured the first direct photograph of the twisted ladder that props up life.

Enzo Di Fabrizio, a physics professor at the Magna Graecia University in Catanzaro, Italy, snapped the picture using an electron microscope.

Previously, scientists had only seen DNA's structure indirectly. The double-corkscrew form was first discovered using a technique called X-ray crystallography, in which a material's shape is reconstructed based on how X-rays bounce after they collide with it.

But Di Fabrizio and his colleagues developed a plan to bring DNA out of hiding. They built a nanoscopic landscape of extremely water-repellant silicon pillars. When they added a solution that contained strands of DNA into this scene, the water quickly evaporated and left behind cords of bare DNA that stretched like tightropes between the tiny mesas.

They then shone beams of electrons through holes in the silicon bed, and captured high-resolution images of the illuminated molecules.

Di Fabrizio's images actually show a thread of several interwoven DNA molecules, as opposed to just two coupled strands. This is because the energy of the electrons used would be enough to destroy an isolated double helix, or a single strand from a double helix.

But with the use of more sensitive equipment and lower energy electrons, Di Fabrizio thinks that snapshots of individual double helices will soon be possible, reports New Scientist.

Molecules of DNA, or deoxyribonucleic acid, store the genetic instructions that govern all living organisms' growth and function.

Di Fabrizio's innovation will allow scientists to vividly observe interactions between DNA and some of life's other essential ingredients, such as RNA (ribonucleic acid). The results of Di Fabrizio's work were published in the journal NanoLetters.

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DNA Directly Photographed for First Time