IMAGE:This is an artist's impression of shape-complementary DNA components that self-assemble into nanoscale machinery. view more <\/p>\n
Credit: C. Hohmann \/ NIM <\/p>\n
The latest DNA nanodevices created at the Technische Universitaet Muenchen (TUM) - including a robot with movable arms, a book that opens and closes, a switchable gear, and an actuator - may be intriguing in their own right, but that's not the point. They demonstrate a breakthrough in the science of using DNA as a programmable building material for nanometer-scale structures and machines. Results published in the journal Science reveal a new approach to joining - and reconfiguring - modular 3D building units, by snapping together complementary shapes instead of zipping together strings of base pairs. This not only opens the way for practical nanomachines with moving parts, but also offers a toolkit that makes it easier to program their self-assembly. <\/p>\n
The field popularly known as \"DNA origami,\" in reference to the traditional Japanese art of paper folding, is advancing quickly toward practical applications, according to TUM Prof. Hendrik Dietz. Earlier this month, Dietz was awarded Germany's most important research award, the Gottfried Wilhelm Leibniz Prize, for his role in this progress. <\/p>\n
In recent years, Dietz and his team have been responsible for major steps in the direction of applications: experimental devices including a synthetic membrane channel made from DNA; discoveries that cut the time needed for self-assembly processes from a week to a few hours and enable yields approaching 100%; proof that extremely complex structures can be assembled, as designed, with subnanometer precision. <\/p>\n
Yet all those advances employed \"base-pairing\" to determine how individual strands and assemblies of DNA would join up with others in solution. What's new is the \"glue.\" <\/p>\n
\"Once you build a unit with base pairs,\" Dietz explains, \"it's hard to break apart. So dynamic structures made using that approach tended to be structurally simple.\" To enable a wider range of DNA nanomachines with moving parts and potentially useful capabilities, the team adapted two more techniques from nature's biomolecular toolkit: the way proteins use shape complementarity to simplify docking with other molecules, and their tendency to form relatively weak bonds that can be readily broken when no longer needed. <\/p>\n
Bio-inspired flexibility <\/p>\n
For the experiments reported in Science, Dietz and his co-authors - doctoral candidates Thomas Gerling and Klaus Wagenbauer, and bachelor's student Andrea Neuner from TUM's Munich School of Engineering - took inspiration from a mechanism that allows nucleic acid molecules to bond through interactions weaker than base-pairing. In nature, weak bonds can be formed when the RNA-based enzyme RNase P \"recognizes\" so-called transfer RNA; the molecules are guided into close enough range, like docking spacecraft, by their complementary shapes. <\/p>\n
The new technology from Dietz's lab imitates this approach. To create a dynamic DNA nanomachine, the researchers begin by programming the self-assembly of 3D building blocks that are shaped to fit together. A weak, short-ranged binding mechanism called nucleobase stacking can then be activated to snap these units in place. Three different methods are available to control the shape and action of devices made in this way. <\/p>\n
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More: IMAGE:This is an artist's impression of shape-complementary DNA components that self-assemble into nanoscale machinery. Continue reading
\nDesigner's toolkit for dynamic DNA nanomachines<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"