BACKGROUND<\/p>\n
T cells which produce interleukin (IL)-<\/b>17 are involved in chronic inflammatory processes and regulatory T (Treg) cells are possibly the most important immune regulators. We aimed to investigate peripheral blood IL-17+<\/sup> T and Foxp3+<\/sup> Treg cells in women with idiopathic recurrent pregnancy loss (RPL).<\/p>\n METHODS<\/p>\n The study design is a cross-sectional evaluation of Th1, Th2, IL-17+<\/sup> T and Treg cells in women with idiopathic RPL (n<\/I> = 42) and age-matched parous controls (n<\/I> = 24). Flow cytometric analysis was performed to measure IL-17+<\/sup> T and Foxp3+<\/sup> Treg cells, and ratios of Th1\/Th2 cells using anti-IL-17A and anti-Foxp3 antibodies, and monoclonal antibodies to tumor necrosis factor (TNF)-α, interferon (IFN)- and IL-10. Student's t<\/I>-test and partial correlations were applied for statistical analysis.<\/p>\n RESULTS<\/p>\n TNF-α-\/IL-10-producing CD3+<\/sup>CD4+<\/sup> T cell ratio was higher in women with RPL than controls (P<\/I> = 0.048). Levels of IL-17+<\/sup> T cells (P<\/I> = 0.021) and the IL-17+<\/sup> T\/CD4+<\/sup>Foxp3+<\/sup> Treg cell ratio (P<\/I> = 0.001) were increased, whereas Foxp3+<\/sup> (P<\/I> = 0.035), Foxp3low<\/sup> (P<\/I> = 0.032) and CD4+<\/sup>Foxp3+<\/sup> T cell (P<\/I> = 0.037) levels were decreased in women with RPL, compared with controls. Levels of IL-17+<\/sup> T cells were correlated with TNF-α-producing CD3+<\/sup>CD4+<\/sup> T cells (r<\/I> = 0.269, P<\/I> = 0.033), and with ratios of TNF-α\/IL-10 (r<\/I> = 0.276, P<\/I> = 0.027) and IFN-\/IL-10 (r<\/I> = 0.266, P<\/I> = 0.035)-producing CD3+<\/sup>CD4+<\/sup> cells. Furthermore, the ratio of IL-17+<\/sup> T cells to CD4+<\/sup>Foxp3+<\/sup> Treg cells showed a positive correlation with TNF-α-producing CD3+<\/sup>CD4+<\/sup> T cells (P<\/I> = 0.047) and IFN--producing CD3+<\/sup>CD4+<\/sup> T cells (P<\/I> = 0.048) as well as a ratio of IFN-\/IL-10-producing CD3+<\/sup>CD4+<\/sup> T cells (P<\/I> = 0.037).<\/p>\n CONCLUSIONS<\/p>\n Enhanced pro-inflammatory immune responses with suppressed immune regulation may be an important immune mechanism involved in RPL.<\/p>\n Source: BACKGROUND T cells which produce interleukin (IL)-17 are involved in chronic inflammatory processes and regulatory T (Treg) cells are possibly the most important immune regulators. We aimed to investigate peripheral blood IL-17+ T and Foxp3+ Treg cells in women with … Continue reading
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