Public release date: 24-Oct-2012 [ | E-mail | Share ] <\/p>\n
Contact: Jim Newman newmanj@ohsu.edu<\/a> 503-494-8231 Oregon Health & Science University<\/p>\n PORTLAND, Ore. - Oregon Health & Science University's development of a new gene therapy method to prevent certain inherited diseases has reached a significant milestone. Researchers at the university's Oregon National Primate Research Center and the OHSU Department of Obstetrics & Gynecology have successfully demonstrated their procedure in human cells. It's believed that this research, along with other efforts, will pave the way for future clinical trials in human subjects. <\/p>\n The research results are online Wednesday, Oct. 24, in the highly respected journal Nature. Dr. Mitalipov also will present the results of his research at the American Society for Reproductive Medicine Conference in San Diego Oct. 24'. <\/p>\n The OHSU gene therapy method was initially devised through research in nonhuman primates led by Shoukhrat Mitalipov, Ph.D., associate scientist in the Division of Reproductive & Developmental Sciences at ONPRC, Oregon Stem Cell Center and OHSU School of Medicine departments of Obstetrics and Gynecology and Molecular and Medical Genetics. <\/p>\n The procedure was specifically developed to prevent diseases related to gene defects in the cell mitochondria. Mitalipov's previous work was published in the August 2009 edition of Nature. In the current study, Mitalipov, in collaboration with Paula Amato, M.D., associate professor of obstetrics and gynecology in the OHSU Center for Women's Health, demonstrated efficacy of this therapy in human gametes and embryos. <\/p>\n \"Cell mitochondria contain genetic material just like the cell nucleus and these genes are passed from mother to infant,\" explained Mitalipov. \"When certain mutations in mitochondrial DNA are present, a child can be born with severe conditions, including diabetes, deafness, eye disorders, gastrointestinal disorders, heart disease, dementia and several other neurological diseases. Because mitochondrial-based genetic diseases are passed from one generation to the next, the risk of disease is often quite clear. The goal of this research is to develop a therapy to prevent transmission of these disease-causing gene mutations.\" <\/p>\n To conduct this research, Mitalipov and his colleagues obtained 106 human egg cells from study volunteers recruited through OHSU's Division of Fertility and Reproductive Endocrinology. The researchers then used a method developed in previous nonhuman primate studies, to transfer the nucleus from one cell to another. In effect, the researchers \"swapped out\" the cell cytoplasm, which contains the mitochondria. The egg cells were then fertilized to determine whether the transfer was a success and whether the cells developed normally. Upon inspection, it was demonstrated that it was possible to successfully replace mitochondrial DNA using this method. <\/p>\n \"Using this process, we have shown that mutated DNA from the mitochondria can be replaced with healthy copies in human cells,\" explained Mitalipov. \"While the human cells in our study only allowed to develop to the embryonic stem cell stage, this research shows that this gene therapy method may well be a viable alternative for preventing devastating diseases passed from mother to infant.\" <\/p>\n The current Nature paper also expanded upon the previously reported nonhuman primate work by demonstrating that the method was possible using frozen egg cells. Mitochondria were replaced in a frozen\/thawed monkey egg cell, resulting in the birth of a healthy baby monkey named Chrysta. <\/p>\n<\/p>\n See original here:<\/p>\n OHSU researchers test new gene therapy method in human cells... and it works<\/a><\/p>\n","protected":false},"excerpt":{"rendered":" Public release date: 24-Oct-2012 [ | E-mail | Share ] Contact: Jim Newman newmanj@ohsu.edu<\/a> 503-494-8231 Oregon Health & Science University PORTLAND, Ore. - Oregon Health & Science University's development of a new gene therapy method to prevent certain inherited diseases has reached a significant milestone. Continue reading