{"id":245362,"date":"2011-02-14T13:46:08","date_gmt":"2011-02-14T13:46:08","guid":{"rendered":"http:\/\/eugenesis.com\/development-and-evaluation-of-a-virtual-microscopy-application-for-automated-assessment-of-ki-67-expression-in-breast-cancer\/"},"modified":"2011-02-14T13:46:08","modified_gmt":"2011-02-14T13:46:08","slug":"development-and-evaluation-of-a-virtual-microscopy-application-for-automated-assessment-of-ki-67-expression-in-breast-cancer","status":"publish","type":"post","link":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/pathology\/development-and-evaluation-of-a-virtual-microscopy-application-for-automated-assessment-of-ki-67-expression-in-breast-cancer.php","title":{"rendered":"Development and evaluation of a virtual microscopy application for automated assessment of Ki-67 expression in breast cancer"},"content":{"rendered":"
The aim of the study was to develop a virtual microscopy enabled method for assessment of Ki-67 expression and to study the prognostic value of the automated analysis in a comprehensive series of patients with breast cancer. <\/div><\/div>

Methods: Using a previously reported virtual microscopy platform and an open source image processing tool, ImageJ<\/em>, a method for assessment of immunohistochemically (IHC) stained area and intensity was created. A tissue microarray (TMA) series of breast cancer specimens from 1931 patients was immunostained for Ki-67, digitized with a whole slide scanner and uploaded to an image web server.<\/p>

The extent of Ki-67 staining in the tumour specimens was assessed both visually and with the image analysis algorithm. The prognostic value of the computer vision assessment of Ki-67 was evaluated by comparison of distant disease-free survival in patients with low, moderate or high expression of the protein. <\/p>

Results: 1648 evaluable image files from 1334 patients were analysed in less than two hours<\/em>.<\/p>

Visual and automated Ki-67 extent of staining assessments showed a percentage agreement of 87% and weighted kappa value of 0.57. The hazard ratio for distant recurrence for patients with a computer determined moderate Ki-67 extent of staining was 1.77 (95% CI 1.31-2.37) and for high extent 2.34 (95% CI 1.76-3.10), compared to patients with a low extent.<\/p>

In multivariate survival analyses, automated assessment of Ki-67 extent of staining was retained as a significant prognostic factor. <\/p>

Conclusions: Running high-throughput automated IHC algorithms on a virtual microscopy platform is feasible. Comparison of visual and automated assessments of Ki-67 expression shows moderate agreement.<\/p>

In multivariate survival analysis, the automated assessment of Ki-67 extent of staining is a significant and independent predictor of outcome in breast cancer.<\/p>

Author: Juho KonstiMikael LundinHeikki JoensuuTiina LehtimakiHarri SihtoKaija HolliTaina Turpeenniemi-HujanenVesa KatajaLiisa SailasJorma IsolaJohan Lundin<\/p>

Credits\/Source: BMC Clinical Pathology 2011,11:3<\/p><\/div>

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<\/p>","protected":false},"excerpt":{"rendered":"

The aim of the study was to develop a virtual microscopy enabled method for assessment of Ki-67 expression and to study the prognostic value of the automated analysis in a comprehensive series of patients with breast cancer. Methods: Using a previously … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":57,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"limit_modified_date":"","last_modified_date":"","_lmt_disableupdate":"","_lmt_disable":"","footnotes":""},"categories":[577487],"tags":[],"class_list":["post-245362","post","type-post","status-publish","format-standard","hentry","category-pathology"],"modified_by":null,"_links":{"self":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/posts\/245362"}],"collection":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/users\/57"}],"replies":[{"embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/comments?post=245362"}],"version-history":[{"count":0,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/posts\/245362\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/media?parent=245362"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/categories?post=245362"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/tags?post=245362"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}