Public release date: 18-Mar-2013 [ | E-mail | Share ] <\/p>\n
Contact: Bernhard Hovemann Bernhard.Hovemann@rub.de<\/a> 49-234-322-4235 Ruhr-University Bochum<\/p>\n Bochum, 18.3.2013 <\/p>\n In the fruit fly Drosophila, the functions of the three enzymes Tan, Ebony and Black are closely intertwined - among other things they are involved in neurotransmitter recycling for the visual process. RUB researchers from the Department of Biochemistry showed for the first time that flies cannot see without this recycling. Their analysis of the enzyme Black also raises new questions as to its function. Anna Ziegler, Florian Brsselbach and Bernhard Hovemann report in the Journal of Comparative Neurology\", which chose this topic as cover story. <\/p>\n Tan, Ebony and Black are important for the visual process and the formation of the cuticle <\/p>\n The fruit fly's genes tan, ebony and black contain the construction plans for three enzymes with the same names that work together in hardening the outer shell of the body, the cuticle. The same enzymes also occur in the compound eye of the fly. Researchers therefore assume that Tan, Ebony and Black work together in vision - similar to the way they do in the formation of the cuticle. In fact, flies with mutations of the ebony and tan genes cannot see. A mutation of the black gene, however, has no such effect. Prof. Hovemann's team examined where the enzyme Black appears in the compound eye and the role it plays in vision. <\/p>\n Black and Ebony always occur together <\/p>\n First, the scientists tested where the genes ebony and black are active in the compound eye of the fruit fly and in its extra eyes on the head, the ocelli. They put different types of light-sensitive cells called photoreceptors, under the microscope. The result: both genes are always read together - just like in the cuticle. This suggests that the functions of the enzymes Ebony and Black are closely linked. <\/p>\n Vision requires a continuous flow of the neurotransmitter histamine <\/p>\n When light falls into the compound eye, the photoreceptors release the neurotransmitter histamine. In previous studies, Bochum's biochemists already demonstrated that histamine is recycled via the glial cells surrounding the photoreceptors. There, the enzyme Ebony inactivates the neurotransmitter histamine by binding it to the amino acid -alanine, thus creating -alanyl-histamine. This molecule is transported from the glial cells back into the photoreceptors. Here, -alanine is split off again by the enzyme Tan, and histamine is produced. Previously, it was assumed that the enzyme Black is responsible for producing the -alanine, which is required for the inactivation of histamine. However, if a fly's eye has no functional Black, the visual process still runs normally. Hovemann's team therefore looked into the question of whether there is another supply route for -alanine. They also tested whether the fly eye can get around the recycling of histamine; this would be possible if the photoreceptors could directly reabsorb the released neurotransmitter, without it being inactivated in the glial cells. <\/p>\n <\/p>\n Read more: Public release date: 18-Mar-2013 [ | E-mail | Share ] Contact: Bernhard Hovemann Bernhard.Hovemann@rub.de<\/a> 49-234-322-4235 Ruhr-University Bochum Bochum, 18.3.2013 In the fruit fly Drosophila, the functions of the three enzymes Tan, Ebony and Black are closely intertwined - among other things they are involved in neurotransmitter recycling for the visual process. RUB researchers from the Department of Biochemistry showed for the first time that flies cannot see without this recycling. Their analysis of the enzyme Black also raises new questions as to its function. Continue reading
\nBlind flies without recycling<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"