{"id":1036943,"date":"2013-07-12T20:00:00","date_gmt":"2013-07-13T00:00:00","guid":{"rendered":"http:\/\/www.immortalitymedicine.tv\/biotechnology\/heterotrophic-growth-of-neochloris-oleoabundans-using-glucose-as-a-carbon-source.php"},"modified":"2024-08-17T16:01:09","modified_gmt":"2024-08-17T20:01:09","slug":"heterotrophic-growth-of-neochloris-oleoabundans-using-glucose-as-a-carbon-source","status":"publish","type":"post","link":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/biotechnology\/heterotrophic-growth-of-neochloris-oleoabundans-using-glucose-as-a-carbon-source.php","title":{"rendered":"Heterotrophic growth of Neochloris oleoabundans using glucose as a carbon source"},"content":{"rendered":"

Background:
\nIn comparison with phototrophic growth, heterotrophic conditions can significantly increase growth rates, final cell number and cell mass in microalgae cultures. Neochloris oleoabundans is a microalga of biotechnological interest that accumulates lipids under phototrophic and nitrogen-limited conditions. Heterotrophic flask culture experiments were conducted to identify carbon sources that can be metabolized by N. oleoabundans, and bioreactor batch and fed-batch (nitrate pulse additions) cultures supplemented with glucose were performed to study the cellular composition of the microalgae under balanced and high C\/N ratios (glucose\/nitrate).
\nResults:
\nN. oleoabundans was able to grow using glucose and cellobiose as sole carbon sources under strict heterotrophic conditions. Under a balanced C\/N ratio of 17 and using bioreactor batch cultures containing 3 g\/L glucose, a maximal cell mass of 1.72 g\/L was found, with protein being the major cell component (44% w\/w). A maximal cell mass of 9.2 g\/L was obtained using batch cultures at a C\/N ratio of 278. Under these conditions, lipid accumulation was promoted (up to 52% w\/w) through N-limitation, resulting in high lipid productivity (528.5 mg\/L\/day). Fed-batch cultures were performed at a C\/N ratio of 278 and with nitrate pulse additions. This condition allowed a maximal cell mass of 14.2 g\/L to be achieved and switched the metabolism to carbohydrate synthesis (up to 54% of dry weight), mainly in the form of starch. It was found that transmembrane transport under these conditions was dependent on a proton-motive force, indicating that glucose is transported by a symporter.
\nConclusions:
\nN. oleoabundans was able to grow under strict heterotrophic culture conditions with glucose or cellobiose as the only carbon source. The glucose used is transported by a symporter system. Batch cultures with a balanced C\/N ratio accumulate proteins as the major cellular component; a high C\/N ratio significantly increased the dry cell mass and resulted in a high lipid content, and a high cell density was achieved using fed-batch cultures promoting carbohydrate accumulation. These results suggest heterotrophic batch cultures of N. oleoabundans as an alternative for the production of proteins or lipids with simple culture strategies and minimal-mineral media supplemented with glucose.Source:
http:\/\/www.biotechnologyforbiofuels.com\/content\/6\/1\/100<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Background: In comparison with phototrophic growth, heterotrophic conditions can significantly increase growth rates, final cell number and cell mass in microalgae cultures. Neochloris oleoabundans is a microalga of biotechnological interest that accumulates lipids under phototrophic and nitrogen-limited conditions. Heterotrophic flask … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"limit_modified_date":"","last_modified_date":"","_lmt_disableupdate":"","_lmt_disable":"","footnotes":""},"categories":[1246860],"tags":[],"class_list":["post-1036943","post","type-post","status-publish","format-standard","hentry","category-biotechnology"],"modified_by":null,"_links":{"self":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/posts\/1036943"}],"collection":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/comments?post=1036943"}],"version-history":[{"count":0,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/posts\/1036943\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/media?parent=1036943"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/categories?post=1036943"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.euvolution.com\/futurist-transhuman-news-blog\/wp-json\/wp\/v2\/tags?post=1036943"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}