STUDY QUESTION<\/p>\n
What are the associations between semen apoptotic M540 bodies and other parameters of semen quality and sonographic alterations of the male genital tract in a cohort of infertile subjects?<\/p>\n
SUMMARY ANSWER<\/p>\n
In infertile subjects, semen M450 bodies are highly correlated with ultrasound and clinical signs of testis abnormalities but not with alterations of other parts of the male genital tract, suggesting a testicular origin of M540 bodies.<\/p>\n
WHAT IS KNOWN ALREADY<\/p>\n
We have reported the presence in semen of round anucleate elements, named ‘M540 bodies’, resembling apoptotic bodies as they contain several apoptotic markers.<\/p>\n
STUDY DESIGN AND SIZE<\/p>\n
A consecutive series of 130 males with couple infertility were evaluated, during the same day session, for clinical, scrotal and transrectal color-Doppler ultrasound characteristics, and hormonal and semen parameters, including interleukin 8 (sIL-8) and M540 body levels.<\/p>\n
PARTICIPANTS\/MATERIALS, SETTING METHODS<\/p>\n
Semen parameters were analyzed by WHO recommended procedures. CDU was performed using the ultrasonographic console Hitachi H21. sIL-8 and serum hormones were evaluated by ELISA methods.<\/p>\n
MAIN RESULTS AND THE ROLE OF CHANCE<\/p>\n
The average percentage value of M540 bodies was 24.6 ± 18.3. After adjusting for possible confounders (age, waist, calculated free testosterone and smoking habit), M450 body levels negatively correlated with sperm number\/ejaculate, progressive motility, normal morphology and sIL-8 levels (adj.r<\/I> = –0.455, P<\/I> < 0.0001; adj.r<\/I> = –0.464, P<\/I> < 0.0001; adj.r<\/I> = –0.430, P<\/I> < 0.001; adj.r<\/I> = –0.236, P<\/I> < 0.05, respectively). In a subset of patients with a history of cryptorchidism (n<\/I> = 8), M540 bodies were higher than in non-cryptorchid men (40.5 ± 14.8 versus 23.6 ± 18.2%; P<\/I> < 0.02). A negative correlation was found between M540 and ultrasound testis volume (adj.r<\/I> = –0.241, P<\/I> < 0.05), whereas a positive association was found with testis inhomogeneity [HR = 1.06 (1.02–1.09); P<\/I> = 0.002], hypoechogenicity [HR = 1.05 (1.01–1.08); P<\/I> < 0.02] and FSH levels (adj.r<\/I> = 0.309, P<\/I> < 0.01). No relationships were found with CDU characteristic of the prostate, seminal vesicles, epididymis and vas deferens. In a multivariate model, testis inhomogeneity and history of cryptorchidism were independently associated with M540 body levels (adj.r<\/I> = 0.355, P<\/I> < 0.01 and adj.r<\/I> = 0.223, P<\/I> < 0.05, respectively). Receiver operating characteristic analysis demonstrated that at the threshold of 27%, M540 bodies discriminate subjects with testis inhomogeneity with a sensitivity of 72% and specificity of 73%.<\/p>\n
LIMITATIONS, REASONS FOR CAUTION<\/p>\n
The increased M540 body semen levels in men with a history of cryptorchidism should be confirmed in a larger number of patients.<\/p>\n
WIDER IMPLICATIONS OF THE FINDINGS<\/p>\n
M540 bodies may be considered a semen marker of altered testis function and thus their evaluation may be helpful in the diagnosis of male infertility.<\/p>\n
STUDY FUNDING\/COMPETING INTEREST(S)<\/p>\n
This work was supported by grants from Ministry of University and Scientific Research (Prin project to E.B. and FIRB project to S.M.) and Regione Toscana (to G.F.).<\/p>\n
Source: STUDY QUESTION What are the associations between semen apoptotic M540 bodies and other parameters of semen quality and sonographic alterations of the male genital tract in a cohort of infertile subjects? SUMMARY ANSWER In infertile subjects, semen M450 bodies are … Continue reading
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