BACKGROUND<\/p>\n
The aim of this study was to investigate the role of the chemokine (C-X-C motif) ligand 12\/chemokine (C-X-C motif) receptor 4 (CXCL12\/CXCR4) axis on the crosstalk between human first-trimester trophoblast cells (TCs) and decidual stromal cells (DSCs), to contribute to a better understanding of the molecular mechanisms on the interaction between the mother and embryo during pregnancy.<\/p>\n
METHODS<\/p>\n
CXCR4 on human first-trimester DSC membranes was detected by flow cytometry. The effects of exogenous CXCL12 or TC-conditioned medium (TCM) on proliferation and invasion of DSCs were examined by measuring proliferating cell nuclear antigen (PCNA) and an invasion assay, respectively. Finally, a co-culture model was established to investigate the effect of CXCL12 secreted from TCs on motility of DSCs.<\/p>\n
RESULTS<\/p>\n
The mean (±SEM) percentage of DSCs positive for CXCR4 was 32.32 ± 7.18%. Human recombinant CXCL12 induced an increase in CXCR4 levels on DSCs via binding to CXCR4 (P<\/I> < 0.01) but had no effect on the PCNA expression of DSCs. Moreover, both exogenous CXCL12 and TCM reinforced the invasive ability of DSCs via CXCR4 ligation. A co-culture model further confirmed that the enhanced invasiveness of DSCs in co-culture with TCs was inhibited by anti-CXCR4 or anti-CXCL12 neutralizing antibody (both P<\/I>< 0.01).<\/p>\n
CONCLUSIONS<\/p>\n
Human first-trimester DSCs express membrane CXCR4 and TC-derived CXCL12 promotes CXCR4 expression and invasion of DSCs via ligation with CXCR4. Our data highlight the role of CXCL12\/CXCR4 axis on the co-operation between TCs and DSCs during human first-trimester pregnancy.<\/p>\n
Source: BACKGROUND The aim of this study was to investigate the role of the chemokine (C-X-C motif) ligand 12\/chemokine (C-X-C motif) receptor 4 (CXCL12\/CXCR4) axis on the crosstalk between human first-trimester trophoblast cells (TCs) and decidual stromal cells (DSCs), to contribute … Continue reading
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